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Testis-specific production and its physiological significance of PACAP

Research Project

Project/Area Number 21591182
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Endocrinology
Research InstitutionKagoshima University

Principal Investigator

MIYATA Atsuro  鹿児島大学, 医歯学総合研究科, 教授 (60183969)

Co-Investigator(Kenkyū-buntansha) INOUE Kazuhiko  鹿児島大学, 医歯学総合研究科, 助教 (30363641)
Co-Investigator(Renkei-kenkyūsha) KANGAWA Kenji  国立循環器病センター研究所, 所長 (00112417)
SHIODA Seiji  昭和大学, 医学部, 教授 (80102375)
HANDA Hiroshi  東京工業大学, 生命理工学研究, 教授 (80107432)
Research Collaborator TOMINAGA Aiko  鹿児島大学, 医歯学総合研究科, 大学院生
SAITO Hiroki  鹿児島大学, 医歯学総合研究科, 大学院生
TAKENOUCHI Rina  鹿児島大学, 医歯学総合研究科, 大学院生
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords生殖内分泌学 / PACAP / 神経ペプチド / 遺伝子発現 / PAC1 / 下垂体 / アデニル酸シクラーゼ / 精巣 / 転写因子 / 生殖系細胞
Research Abstract

Pleiotrophic neuropeptide, pituitary adenylate cyclase activating polypeptide(PACAP) is abundantly localized in testis. It was reported that gene expression of PACAP and its specific receptor PAC1 are periodically changed according to stage of spermatogenesis, suggesting its involvement in gonadal function. We attempted to clarify the testis specific regulatory mechanism of PACAP gene expression, and identified the promoter region responsible for testicular specific expression in 5' upstream region of human testicular specific exon. In addition, we also identified PARP-1 and TIAR as a protein which specifically bind this region. Knock down of them with specific siRNA changed the testis specific promoter activity, suggesting that these proteins might be involved in testis specific function of PACAP.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (50 results)

All 2012 2011 2010 2009 Other

All Journal Article (13 results) (of which Peer Reviewed: 11 results) Presentation (37 results)

  • [Journal Article] N-arachidonoyl glycine induces macrophage apoptosis via GPR182012

    • Author(s)
      Takenouchi R, Inoue K, Kambe Y, Miyata A
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 418 Issue: 2 Pages: 366-71

    • DOI

      10.1016/j.bbrc.2012.01.027

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Role of mitochondrial activation in PACAP-dependent neurite outgrowth2012

    • Author(s)
      Kambe Y, Miyata A
    • Journal Title

      J.Mol.Neurosci.

      Volume: (in press) Issue: 3 Pages: 550-557

    • DOI

      10.1007/s12031-012-9754-0

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of the testis-specific promoter region in the human pituitary adenylate cyclase-activating polypeptide(PACAP) gene2011

    • Author(s)
      Tominaga A, Sugawara H, Futagawa T, Inoue K, Sasaki K, Minamino N, Hatakeyama M, Handa H, Miyata A.
    • Journal Title

      Genes to Cells

      Volume: 15(6) Pages: 595-606

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Regio-and stereoselective oxidatin of propranolol enantiomers by human CYP2D6, cynomolgus monkey CYP2D17 and marmoset CYP2D192011

    • Author(s)
      Narimatsu S, Makata T, Miyata A, Hanioka N, 12人中11番目
    • Journal Title

      Chemico-Biological Interactions

      Volume: 189 Issue: 3 Pages: 146-152

    • DOI

      10.1016/j.cbi.2010.12.014

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] VIP/PACAPと関連疾患2011

    • Author(s)
      宮田篤郎、國師恵美子
    • Journal Title

      医薬ジャーナル

      Volume: 47 Pages: 111-115

    • Related Report
      2011 Annual Research Report
  • [Journal Article] Alternative Splicing of the Pituitary Adenylate Cyclase-activating Polypetide(PACAP) Receptor Contributes to Function of PACAP-272010

    • Author(s)
      Ushiyama M, Ikeda R, Yoshida M, Mori K, Kangawa K, Sugawara H, Inoue K, Yamada K, Miyata A.
    • Journal Title

      Journal of Molecular Neuroscience

      Volume: 42(3) Pages: 341-348

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Alternative Splicing of the Pituitary Adenylate Cyclase-activating Poly petide(PACAP)Receptor Contributes to Function of PACAP-27.2010

    • Author(s)
      Ushiyama M, Ikeda R, Miyata A, (9人中9番目)
    • Journal Title

      Journal of Molecular Neuroscience

      Volume: 42(3) Pages: 341-348

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The effects of the phosphodiesterase inhibitor olprinone on global cerebral ischemia.2010

    • Author(s)
      Okayama N, Miyata A, Kanmura Y, (9人中8番目)
    • Journal Title

      Anesthesia and Analgesia

      Volume: 110(3) Pages: 888-894

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of the testis-specific promoter region in the human pituitary adenylate cyclase-activating polypeptide(PACAP)gene.2010

    • Author(s)
      Tominaga A, Inoue K, Miyata A, (9人中9番目)
    • Journal Title

      Genes to Cells

      Volume: 15(6) Pages: 595-606

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] PACAP/VIP受容体の構造と機能の多様性2010

    • Author(s)
      宮田篤郎, 三浦綾子
    • Journal Title

      医学のあゆみ

      Volume: 233 Pages: 928-933

    • Related Report
      2010 Annual Research Report
  • [Journal Article] Characterization of the testis-specific promoter region in the human pituitary adenylate cyclase-activating polypeptide(PACAP)gene2010

    • Author(s)
      Tominaga Aiko
    • Journal Title

      Genes to Cells (印刷中)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] cAMP activation by PACAP/VIP stimulates IL-6 release and inhibits osteoblastic differentiation through VPAC2 receptor in osteoblastic MC3T3 cells2009

    • Author(s)
      Nagata Azusa
    • Journal Title

      Journal of Cell Physiology 221

      Pages: 75-83

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Regulatory mechanism of PAC1 gene expression via Sp1 by Nerve Growth Factor in PC12 cells

    • Author(s)
      Miura A, Odahara N, Tominaga A, Inoue K, Kambe Y, Kurihara T, Miyata A.
    • Journal Title

      FEBS Letters

      Volume: (in press)

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Presentation] N-arachidonoyl glycine (NAGly)により誘導されるマクロファージのアポトーシスはGPR18を介している2012

    • Author(s)
      竹ノ内里奈、井上和彦、神戸悠輝、栗原崇、宮田篤郎
    • Organizer
      日本薬理学会年会
    • Place of Presentation
      京都市
    • Related Report
      2011 Annual Research Report
  • [Presentation] Lysophosphatidylserine P promotes the Secreion of Growth Hormone in Rat Pituitary Cells via G protein-coupled Receptor2011

    • Author(s)
      R.Takenouchi, K.Inoue, E.Kokushi, M.Yamato, Y.Kambe, T.Kurihara, A.Miyata
    • Organizer
      GPCR Satellite Symposium of International Symposium on VIP-PACAP and Related Peptides
    • Place of Presentation
      イスラエル・エイラト
    • Year and Date
      2011-12-13
    • Related Report
      2011 Annual Research Report
  • [Presentation] リゾフォスファチジルセリンのラット下垂体由来細胞における成長ホルモン分泌への促進効果に関する研究2011

    • Author(s)
      國師恵美子、竹ノ内里奈、大和麻耶、井上和彦、神戸悠輝、宮田篤郎
    • Organizer
      薬物活性シンポジウム
    • Place of Presentation
      福岡市
    • Year and Date
      2011-11-21
    • Related Report
      2011 Annual Research Report
  • [Presentation] カゼインキナーゼ1δ/ε活性化は炎症性疼痛情報伝達に寄与する2011

    • Author(s)
      栗原崇、櫻井絵里、朝田俊秀、吉村恵、宮田篤郎、田邊勉
    • Organizer
      日本薬理学会西南部会
    • Place of Presentation
      福岡市
    • Year and Date
      2011-11-20
    • Related Report
      2011 Annual Research Report
  • [Presentation] Functional analysis of N-arachidonoyl Glycine as anovel anti-inflammatory factor in microglia2011

    • Author(s)
      Rina Takenouchi, Kazuhiko Inoue, Maya Yamato, Atsuro Miyata, Annual Meeting of Society for Nueroscience
    • Organizer
      Annual Meeting of Society for Nueroscience
    • Place of Presentation
      米国・ワシントン
    • Year and Date
      2011-11-14
    • Related Report
      2011 Annual Research Report
  • [Presentation] Neuro2a細胞におけるERストレスによるPAC1遺伝子の負の発現調節機序2011

    • Author(s)
      三浦綾子、井上和彦、神戸悠輝、栗原崇、宮田篤郎
    • Organizer
      日本生化学会
    • Place of Presentation
      京都市
    • Year and Date
      2011-09-24
    • Related Report
      2011 Annual Research Report
  • [Presentation] Pituitary adenylate cyclase activating polypeptide in pain transmission2011

    • Author(s)
      宮田篤郎
    • Organizer
      日中合同薬理シンポジウム
    • Place of Presentation
      中国・ウルムチ
    • Related Report
      2011 Final Research Report
  • [Presentation] Pituitary adenylate cyclase activating polypeptide in pain transmission2011

    • Author(s)
      宮田篤郎
    • Organizer
      日中合同薬理シンポジウム
    • Place of Presentation
      中国・ウルムチ(招待講演)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Involvement of mitochondria in PACAPPAC1 dependent neurite outgrowth2011

    • Author(s)
      Yuki Kambe, Kazuhiko Inoue, Takashi Kurihara, Atsuro Miyata
    • Organizer
      10th International Symposium on VIP-PACAP and related peptides
    • Place of Presentation
      イスラエル・エイラト
    • Related Report
      2011 Annual Research Report
  • [Presentation] Sp1 is involved in not only positively but also negatively expression of PAC1 gene2011

    • Author(s)
      Ayako Miura, Yuki Kambe, Kazuhiko Inoue, Takashi Kurihara, Atsuro Miyata
    • Organizer
      10th International Symposium on VIP-PACAP and related peptides
    • Place of Presentation
      イスラエル・エイラト
    • Related Report
      2011 Annual Research Report
  • [Presentation] Lipid biofactor signaling with GPCRs in immune system : anti-inflammatry role of N-arachidonylglycine via GPR182011

    • Author(s)
      Atsuro MIyata
    • Organizer
      Japan-Israel Joint Symposium on G-protein Coupled Receptors
    • Place of Presentation
      イスラエル・エイラト(招待講演)
    • Related Report
      2011 Annual Research Report
  • [Presentation] 中枢神経への高脂肪食の影響に関与する受容体の解析2010

    • Author(s)
      新川純平、神戸悠輝、山田勝士、宮田篤郎, (7人中7番目)
    • Organizer
      日本薬理学会西南部会
    • Place of Presentation
      鹿児島
    • Year and Date
      2010-11-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] PC12細胞におけるERストレスによるPAC1遺伝子の負の発現調節機序2010

    • Author(s)
      三浦綾子、富永愛子、井上和彦、宮田篤郎, (6人中6番目)
    • Organizer
      日本薬理学会西南部会
    • Place of Presentation
      鹿児島
    • Year and Date
      2010-11-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] PACAPの神経特異的発現調節と機能の多様性の解明:臨床への応用展開を目指して2010

    • Author(s)
      宮田篤郎
    • Organizer
      トランスレーショナルリサーチ推進センターセミナー
    • Place of Presentation
      福井
    • Year and Date
      2010-02-15
    • Related Report
      2011 Final Research Report
  • [Presentation] PACAPの神経特異的発現調節と機能の多様性の解明:臨床への応用展開を目指して2010

    • Author(s)
      宮田篤郎
    • Organizer
      トランスレーショナルリサーチ推進センターセミナー
    • Place of Presentation
      福井大学
    • Year and Date
      2010-02-15
    • Related Report
      2009 Annual Research Report
  • [Presentation] 多機能神経ペプチドPACAPの精巣特異的機能発現2010

    • Author(s)
      宮田篤郎、富永愛子、佐々木一樹、南野直人(6人中1番目)
    • Organizer
      第28回内分泌代謝学サマーセミナー
    • Place of Presentation
      長崎
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] Characterization of The testis specific promoter region in humam pituitary adenylate cyclase-activating polypeptide(PACAP) gene.2010

    • Author(s)
      Miyata A, Tominaga A, Inoue K, Minamino N, (6人中1番目)
    • Organizer
      ENDO EXPO 2010
    • Place of Presentation
      San Diego, USA
    • Related Report
      2010 Annual Research Report
  • [Presentation] 痛覚伝達におけるPACAP特異的受容体PAC 1の役割2010

    • Author(s)
      宮田篤郎、大納哲也、坂本光、井上和彦, (6人中1番目)
    • Organizer
      第7回 GPCR研究会
    • Place of Presentation
      東京
    • Related Report
      2010 Annual Research Report
  • [Presentation] PC12細胞において神経保護作用を示すPAC 1遺伝子発現におけるNGFの関与2010

    • Author(s)
      三浦綾子、富永愛子、神戸悠輝、井上和彦、宮田篤郎
    • Organizer
      第7回 GPCR研究会
    • Place of Presentation
      東京
    • Related Report
      2010 Annual Research Report
  • [Presentation] マクロファージにおけるN-arachidonyl glycine(NAGly)の性状解析2010

    • Author(s)
      竹ノ内里奈、井上和彦、神戸悠輝、宮田篤郎
    • Organizer
      第7回 GPCR研究会
    • Place of Presentation
      東京
    • Related Report
      2010 Annual Research Report
  • [Presentation] From mitochondria to nucleus, Pgc-1 translocation may be one of the key factors for neurite extension in N2a cells2010

    • Author(s)
      Kambe Y, Miyata A
    • Organizer
      Annual Meeting of Neuroscience
    • Place of Presentation
      San Diego, USA
    • Related Report
      2010 Annual Research Report
  • [Presentation] Regulatory mechanism of PAC 1 gene expression by nerve growth factor (NGF) in PC12 cells2010

    • Author(s)
      Miura A, Tominaga A, Inoue K, Kambe Y, Miyata A
    • Organizer
      Annual Meeting of Neuroscience
    • Place of Presentation
      San Diego, USA
    • Related Report
      2010 Annual Research Report
  • [Presentation] Implication of pituitary adenylate cyclase-activating polypeptide (PACAP) in diabethic neuropathy of streptozotocin (STZ) treatedmice2010

    • Author(s)
      Miyata A, Ikeda K, Inoue K, Kurihara T, Shimizu T
    • Organizer
      20^<th>Japan-Korea Joint Seminar on Pharmacology
    • Place of Presentation
      Kagoshima
    • Related Report
      2010 Annual Research Report
  • [Presentation] PC12細胞におけるPAC1遺伝子の正と負の調節機序について2010

    • Author(s)
      三浦綾子、富永愛子、井上和彦、宮田篤郎, (6人中6番目)
    • Organizer
      日本分子生物学会年会・日本生化学大会
    • Place of Presentation
      神戸
    • Related Report
      2010 Annual Research Report
  • [Presentation] 共役リノール酸受容体の同定2010

    • Author(s)
      井上和彦、桐原みらい、竹之内里奈、宮田篤郎, (6人中6番目)
    • Organizer
      日本分子生物学会年会・日本生化学大会
    • Place of Presentation
      神戸
    • Related Report
      2010 Annual Research Report
  • [Presentation] カニクイザル及びマーモセットCYP2B酵素の構造と機能2010

    • Author(s)
      真弓慶、津村泰匡、宮田篤郎、成松鎮雄, (7人中6番目)
    • Organizer
      日本分子生物学会年会・日本生化学大会
    • Place of Presentation
      神戸
    • Related Report
      2010 Annual Research Report
  • [Presentation] Implication of pituitary adenylate cyclase-activating polypeptide (PACAP) in diabethic neuropathy of streptozotocin (STZ) treated mice2010

    • Author(s)
      Miyata A, Ikeda K, Inoue K, Kurihara T, Shimizu T
    • Organizer
      5^<th> International Peptide Symposium
    • Place of Presentation
      Kyoto
    • Related Report
      2010 Annual Research Report
  • [Presentation] PC12細胞において神経保護作用を示すPAC1受容体遺伝子発現におけるNGFの関与2010

    • Author(s)
      三浦綾子
    • Organizer
      第83回日本薬理学会年会
    • Place of Presentation
      大阪
    • Related Report
      2009 Annual Research Report
  • [Presentation] 共益リノール酸(conjugated linoleic acid ; CLA)は長鎖脂肪酸受容体に結合するのか?2010

    • Author(s)
      桐原みらい
    • Organizer
      第83回日本薬理学会年会
    • Place of Presentation
      大阪
    • Related Report
      2009 Annual Research Report
  • [Presentation] マクロファージにおけるリポアミノ酸によるアポトーシス誘導2009

    • Author(s)
      竹ノ内里奈
    • Organizer
      第62回日本薬理学会西南部会
    • Place of Presentation
      松山
    • Year and Date
      2009-11-27
    • Related Report
      2009 Annual Research Report
  • [Presentation] Alternative splicing of the pituitary adenylate cyclase activating polypeptide(PACAP)receptor contribute to function of PACAP-272009

    • Author(s)
      Ushiyama Mina
    • Organizer
      8th International Symposium on VIP, PACAP and Related Peptides
    • Place of Presentation
      鹿児島市
    • Related Report
      2009 Annual Research Report
  • [Presentation] Regulatory mechanism of PAC1 gene expression by nerve growth factor(NGF)in PC12 cells2009

    • Author(s)
      三浦綾子
    • Organizer
      8th International Symposium on VIP, PACAP and Related Peptides
    • Place of Presentation
      鹿児島市
    • Related Report
      2009 Annual Research Report
  • [Presentation] The alternative regulation of pituitary adenylate cyclase activating poly peptide(PACAP)gene expression by neural-restrictive silencer2009

    • Author(s)
      Sugawara Hideki
    • Organizer
      8th International Symposium on VIP, PACAP and Related Peptides
    • Place of Presentation
      鹿児島市
    • Related Report
      2009 Annual Research Report
  • [Presentation] Involvement of pituitary adenylate cyclase activating polypeptide(PACAP)in diabetic neuropathy of streptozotocin(STZ)treated mice2009

    • Author(s)
      Ikeda Keigo
    • Organizer
      8th International Symposium on VIP, PACAP and Related Peptides
    • Place of Presentation
      鹿児島市
    • Related Report
      2009 Annual Research Report
  • [Presentation] Neuromedin B stimulates Proliferation of Rat Calvarial Osteoblasts through ERK Signaling Pathway2009

    • Author(s)
      Saito HIroki
    • Organizer
      Satellte Symposium of 9th International Symposium on VIP, PACAP and Related Peptides
    • Place of Presentation
      Yakushima, Kagoshima
    • Related Report
      2009 Annual Research Report
  • [Presentation] 遊離脂肪酸受容体の新機能 ~敗血症における役割~2009

    • Author(s)
      井上和彦
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      神戸
    • Related Report
      2009 Annual Research Report
  • [Presentation] リポアミノ酸によるアポトーシス誘導機序の解明2009

    • Author(s)
      竹ノ内里奈
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      神戸
    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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