Development of novel identification methods of SNPs responsible for minor H antigens and open public internet software tool

• Project/Area Number: 21591256
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Hematology
• Research Institution: Aichi Cancer Center Research Institute
• Principal Investigator: AKATSUKA Yoshiki 愛知県がんセンター(研究所), 腫瘍免疫学部, 客員研究員 (70333391)
• Co-Investigator(Kenkyū-buntansha): KUZUSHIMA Kiyotaka 愛知県がんセンター研究所, 腫瘍免疫学部, 部長 (30311442)
• Research Collaborator: OGAWA Seishi 東京大学, 医学部附属病院, 特任准教授 (60292900)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 同種造血細胞移植 / 白血病 / 腫瘍免疫 / 遺伝子多型 / 国際HapMapプロジェクト / オンラインソフトウエア / バイオテクノロジー / 免疫学 / SNP / HapMap / パイオテクノロジー

Research Abstract

Minor histocompatibility antigens(H) are polymorphic proteins targeted by donor immune cells to cure hematological malignancies following allogeneic hematopoietic cell transplantation. This immune response utilizes rejection reaction due to genetic disparities between individuals. In this study, we developed a software that can efficiently identify single nucleotide polymorphisms(SNPs) responsible for generation of minor H antigens among large number of SNPs and have made the software available via internet as researcher-friendly interactive manners.

Research Products

• [Journal Article] HapMap SNP Scanner : an online program to mine SNPs responsible for cell phenotype (2012)
  • Author(s): Yamamura T, Hikita J, Bleakley M, Hirosawa T, Sato-Otsubo A, Torikai H, Hamajima T, Nannya Y, Demachi-Okamura A, Maruya E, Saji H, Yamamoto Y, Takahashi T, Emi N, Morishima Y, Kodera Y, Kuzushima K, Riddell SR, Ogawa S, Akatsuka Y.
  • Journal Title: Tissue Antigens Volume: (in press) Issue: 2 Pages: 119-125
  • DOI: 10.1111/j.1399-0039.2012.01883.x
  • Peer Reviewed
• [Journal Article] Glypican-3 could be an effective target for immunotherapy combined with chemotherapy against ovarian clear cell carcinoma (2011)
  • Author(s): Suzuki S, Yoshikawa T, Hirosawa T, Shibata K, Kikkawa F, Akatsuka Y, Nakatsura T.
  • Journal Title: Cancer Sci Volume: 102 Issue: 9 Pages: 1622-9
  • DOI: 10.1111/j.1349-7006.2011.02003.x
  • NAID: 10030242806
  • Peer Reviewed
• [Journal Article] Mismatched human leukocyte antigen class II-restricted CD8^+cytotoxic T cells may mediate selective graft-versus-leukemia effects following allogeneic hematopoietic cell transplantation (2011)
  • Author(s): Hirosawa T, Torikai H, Yanagisawa M, Kamei M, Imahashi N, Demachi-Okamura A, Tanimoto M, Shiraishi K, Ito M, Miyamura K, Shibata K, Kikkawa F, Morishima Y, Takahashi T, Emi N, Kuzushima K, Akatsuka Y.
  • Journal Title: Cancer Sci Volume: 102 Issue: 7 Pages: 1281-6
  • DOI: 10.1111/j.1349-7006.2011.01949.x
  • NAID: 10029295003
  • Peer Reviewed
• [Journal Article] Relapse of leukemia with loss of mismatched HLA resulting from uniparental disomy after haploidentical hematopoietic stem cell transplantation (2010)
  • Author(s): Villalobos IB, Takahashi Y, Akatsuka Y, Muramatsu H, Nishio N, Hama A, Yagasaki H, Saji H, Kato M, Ogawa S, Kojima S.
  • Journal Title: Blood Volume: 115 Issue: 15 Pages: 3158-61
  • DOI: 10.1182/blood-2009-11-254284
  • Peer Reviewed
• [Journal Article] Therapy of relapsed leukemia after allogeneic hematopoietic cell transplantation with T cells specific for minor histocompatibility antigens (2010)
  • Author(s): Warren EH, Fujii N, Akatsuka Y, Chaney CN, Mito JK, Loeb KR, Gooley TA, Brown ML, Koo KK, Rosinski KV, Ogawa S, Matsubara A, Appelbaum FR, Riddell SR.
  • Journal Title: Blood Volume: 115(19) Issue: 19 Pages: 3869-78
  • DOI: 10.1182/blood-2009-10-248997
  • Peer Reviewed
• [Journal Article] Modified ELISPOT assay may predict T-cell hyporesponsiveness to non-inherited maternal antigens (2010)
  • Author(s): Taniguchi K, Shimazaki C, Ochiai N, Maruya E, Akatsuka Y, Ashihara E, Maekawa T, Taniwaki M, Saji H.
  • Journal Title: Int J Lab Hematol Volume: 32 Issue: 1p1
  • DOI: 10.1111/j.1751-553x.2008.01121.x
  • Peer Reviewed
• [Journal Article] Relapse of leukemia with loss of mismatched HLA resulting from uniparental disomy after haploidentical hematopoietic stem cell transplantation. (2010)
  • Author(s): Villalobos IB, et al.
  • Journal Title: Blood Volume: 115 Pages: 3158-3161
  • Peer Reviewed
• [Journal Article] 同種造血幹細胞移植とマイナー組織適合性抗原 (2010)
  • Author(s): 小川誠司, ら
  • Journal Title: 医学のあゆみ Volume: 233 Pages: 1217-1222
• [Journal Article] Japan Marrow Donation Program(JMDP). Exploration of the genetic basis of GVHD by genetic association studies (2009)
  • Author(s): Ogawa S, Matsubara A, Onizuka M, Kashiwase K, Sanada M, Kato M, Nannya Y, Akatsuka Y, Satake M, Takita J, Chiba S, Saji H, Maruya E, Inoko H, Morishima Y, Kodera Y, Takehiko S
  • Journal Title: Biol Blood Marrow Transplant Volume: 15 Issue: 1 Pages: 39-41
  • DOI: 10.1016/j.bbmt.2008.11.020
• [Journal Article] マイナー抗原を標的とした造血器腫瘍に対する免疫療法. (2009)
  • Author(s): 赤塚美樹
  • Journal Title: 血液フロンティア 19 Pages: 559-566
• [Journal Article] Therapy of relapsed leukemia after allogeneic hematopoietic cell transplant with T cells specific for minor histocompatibility antigens.
  • Author(s): Warren EH
  • Journal Title: BLOOD (In press)
  • Peer Reviewed
• [Presentation] An online tool to scan single nucleotide polymorphisms for identification of novel minor antigens (2011)
  • Author(s): katsuka Y
  • Organizer: 第73回日本血液学会学術集会
  • Place of Presentation: 名古屋国際会議場,名古屋市
  • Year and Date: 2011-10-14
• [Presentation] An online tool to scan single nucleotide polymorphisms for identification of novel minor antigens (2011)
  • Author(s): Akatsuka Y, ら
  • Organizer: 第73回日本血液学会学術集会
  • Place of Presentation: 国際会議場(名古屋)(ポスター#PS1-262)
  • Year and Date: 2011-10-14
• [Presentation] マイナー組織適合抗原をコードする一塩基多型のオンライン検索ツール (2011)
  • Author(s): 赤塚美樹
  • Organizer: 第70回日本癌学会総会
  • Place of Presentation: 名古屋国際会議場名古屋市
  • Year and Date: 2011-10-03
• [Presentation] マイナー組織適合抗原をコードする一塩基多型のオンライン検索ツール (2011)
  • Author(s): 赤塚美樹, ら
  • Organizer: 第70回日本癌学会総会
  • Place of Presentation: 国際会議場(名古屋)
  • Year and Date: 2011-10-03
• [Presentation] 同種移植後再発予防・治療を目的としたマイナー抗原ワクチン臨床試験 (2011)
  • Author(s): 赤塚美樹
  • Organizer: 第15回日本がん免疫学会総会
  • Place of Presentation: 千里ライフサイエンスセンター,大阪市
  • Year and Date: 2011-06-30
• [Presentation] 同種移植後再発予防・治療を目的としたマイナー抗原ワクチン臨床試験(中間報告) (2011)
  • Author(s): 赤塚美樹, ら
  • Organizer: 第15回日本がん免疫学会総会
  • Place of Presentation: 千里ライフサイエンスセンタービル(大阪)(口演#39)
  • Year and Date: 2011-06-30
• [Presentation] マイナー組織適合抗原の重要性 (2011)
  • Author(s): 赤塚美樹
  • Organizer: 第33回日本造血細胞移植学会・シンポジウム
  • Place of Presentation: ひめぎんホール(愛媛県)
  • Year and Date: 2011-03-09
• [Presentation] Characterization And Clinical Application of Minor Histocompatibility Antigens (2011)
  • Author(s): Akatsuka Y
  • Organizer: The 15th Annual Winter Meeting of the Korean Society of Blood and Marrow Transplantation
  • Place of Presentation: Muju Resort、Muju, Korea
  • Year and Date: 2011-02-25
• [Presentation] Characterization And Clinical Application of Minor Histocompatibility Antigens. (2011)
  • Author(s): Akatsuka Y, et al.
  • Organizer: The 15th Annual Winter Meeting of the Korean Society of Blood and Marrow Transplantation
  • Place of Presentation: Muju Resort (Muju, Korea)
  • Year and Date: 2011-02-25
• [Presentation] Development of an Online Tool to Scan Single Nucleotide Polymorphisms for Identification of Novel Minor Histocompatibility Antigens (2011)
  • Author(s): Yamamura T, et al
  • Organizer: 第17回BMT Tandem Meetings
  • Place of Presentation: Hawaii Convention Center, Honolulu
  • Year and Date: 2011-02-19
• [Presentation] Development of an Online Tool to Scan Single Nucleotide Polymorphisms for Identification of Novel Minor Histo-compatibility Antigens. (2011)
  • Author(s): Yamamura T, et al.
  • Organizer: 第17回BMT Tandem Meetings
  • Place of Presentation: Hawaii Convention Center (Honolulu, USA)
  • Year and Date: 2011-02-19
• [Presentation] Identification of novel minor histocompatibility antigens using HAPMAP EBV-LCL panels transduced with restricting HLA cDNA retrovirally (2010)
  • Author(s): Akatsuka Y
  • Organizer: 第16回日本遺伝子治療学会総会
  • Place of Presentation: 栃木県総合文化センター,宇都宮市
  • Year and Date: 2010-07-01
• [Presentation] Identification of novel minor histocompatibility antigens using HAPMAP EBV-LCL panels transduced with restricting HLA cDNA retrovirally. (2010)
  • Author(s): Akatsuka Y, et al.
  • Organizer: 第16回日本遺伝子治療学会総会
  • Place of Presentation: 栃木県総合文化センター(栃木県)
  • Year and Date: 2010-07-01
• [Presentation] HLAクラスII不適合骨髄移植患者より樹立した不一致クラスII拘束性アロCD8+CTLクローンの解析 (2009)
  • Author(s): 鳥飼宏基
  • Organizer: 第71回日本血液学会学術集会
  • Place of Presentation: 国立京都国際会館、京都市
  • Year and Date: 2009-10-25
• [Presentation] HLAクラスII不適合骨髄移植患者より樹立した不一致クラスII拘束性アロCD8+CTLクローンの解析 (2009)
  • Author(s): 鳥飼宏基
  • Organizer: 第71回日本血液学会学術集会
  • Place of Presentation: 京都(京都国際会議場)
  • Year and Date: 2009-10-25
• [Presentation] Clinical significance of CD8+CTL clone recognizing mismatched HLA class II (2009)
  • Author(s): Torikai H
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜,横浜市
  • Year and Date: 2009-10-02
• [Presentation] Clinical significance of CD8+ CTL clone recognizing mismatched HLA class II. (2009)
  • Author(s): Torikai H
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: 横浜(パシフィコ横浜)
  • Year and Date: 2009-10-02
• [Presentation] 移植後再発白血病に対するマイナー抗原特異的CTL養子免疫療法後に重症肺GVHDの合併を来した標的抗原の同定 (2009)
  • Author(s): 赤塚美樹
  • Organizer: 第13回日本がん免疫学会総会
  • Place of Presentation: 北九州国際会議場,北九州市
  • Year and Date: 2009-06-24
• [Presentation] 移植後再発白血病に対するマイナー抗原特異的CTL養子免疫療法後に重症肺GVHDの合併を来した標的抗原の同定 (2009)
  • Author(s): 赤塚美樹
  • Organizer: 第13回日本がん免疫学会総会
  • Place of Presentation: 北九州市(北九州国際会議場)
  • Year and Date: 2009-06-24
• [Remarks]
  • URL: http://hapmap-scanner.fujita-hu.ac.jp/
• [Remarks]
  • URL: http://hapmap-scanner.fujita-hu.ac.jp/
• [Remarks] (報告時点では限定公開中)論文受理後に対話型ソフトウエアを公開予定
  • URL: http://hapmap-scanner.fujita-hu.ac.jp/hms_ceu.html
• [Remarks] 論文受理後に対話型ソフトウエアを公開予定