Study for biofilm formed by Streptococcus pneumoniae and the treatment strategy
| Project/Area Number |
21591305
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Infectious disease medicine
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| Research Institution | Kurume University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
QIN Liang 久留米大学, 医学部, 助教 (40461404)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 肺炎球菌 / バイオフィルム / 難治性感染症 |
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| Research Abstract |
There is still limited information about biofilm formed by Streptococcus pneumoniae, as well as the treatment of biofilm diseases is also controversial. To evaluate the characteristics of S. pneumoniae biofilm, we conducted the following study. A new system was designed to investigate the formation of S. pneumoniae biofilm, which includes newly defined biofilm culture condition, biofilm microtiter assay, and the utilities of con focal laser scanning microscopy(CLSM) and scanning electron microscope(SEM). We detected the varieties of S. pneumoniae biofilm existed in the strains with different serotypes and antimicrobial susceptibilities. We also investigated the in vitro expression of genes relevant to antimicrobial resistance, pathogenicity and adherence in the planktonic or biofilm condition, with or without antibiotic treatment(meropenem) for TIGR4 strain. As a result, the higher expression of genes relevant to antimicrobial resistance and pathogenicity was detected in the biofilm condition after meropenem treated. In this study, we designed a new biofilm observation system, and our data indicated that S. pneumoniae is capable to produce biofilm, and many factors such as serotypes, and antimicrobial susceptibilities especially when S. pneumonia is under antibiotics treatment may affect the biofilm formation. All these findings are supposed to be a part of new evidences to establish an effective biofilm diseases treatment strategy.
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Report
(4 results)
Research Products
(134 results)
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[Journal Article] Clinical study concerning the relationship between community-acquired pneumonia and viral infection in northern Thailand2011
Author(s)
Hara K, Yahara K, Gotoh K, Nakazono Y, Kashiwagi T, Imamura Y, Hamada N, Khositsakulchai W, Sanchai T, Khantawa B, Tharavichitkul P, Maneekarn N, Sirisanthana T, and Watanabe H.
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Journal Title
Inter Med
Volume: 50
Pages: 991-998
NAID
Related Report
Peer Reviewed
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[Journal Article] Nosocomial bacteremia caused by biofilmforming Bacillus cereus and Bacillus thuringiensis2009
Author(s)
Kuroki R, Kawakami K, Qin L, Kaji C, Watanabe K, Kimura Y, Ishiguro C, Tanimura S, Tsuchiya Y, Hamaguchi I, Sakakura M, Sakabe S, Tsuji K, Inoue M, and Watanabe H.
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Journal Title
Intern Med
Volume: 48
Pages: 791-796
NAID
Related Report
Peer Reviewed
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[Journal Article] Nosocomial bacteremia caused by biofilm-forming Bacillus cereus and Bacillus thuringiensis2009
Author(s)
Kuroki R, Kawakami K, Qin L, Kaji C, Watanabe K, Kimura Y, Ishiguro C, Tanimura S, Tsuchiya Y, Hamaguchi I, Sakakura M, Sakabe S, Tsuji K, Inoue M, Watanabe H.
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Journal Title
Internal Medicine 48
Pages: 791-796
NAID
Related Report
Peer Reviewed
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[Journal Article]2009
Author(s)
秦亮、渡邊浩
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Journal Title
呼吸器科「インフルエンザ菌 特集-耐性菌感染症」(科学評論社)
Pages: 19-24
Related Report
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[Journal Article]2009
Author(s)
秦亮、渡邊浩
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Journal Title
肺炎球菌ワクチンの使い方。呼吸器感染症のすべて-私の治療のコツ-(南江堂)
Pages: 172-173
Related Report
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[Journal Article]2009
Author(s)
原好勇、渡邊浩
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Journal Title
臨床検査「ヒト・メタニューモウイルス感染症」(医学書院)
Pages: 92-96
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[Presentation] Infection control for the pandemic(H1N1)2009 in Kurume university hospital2010
Author(s)
Watanabe H, Kashiwagi T, Hara K, Masunaga K, Kawano S, Miura M, Iwahashi J, Qin L, Imamura Y, Hamada N.
Organizer
Meeting of the global outbreak alert and response network(GOARN)partners from the western pacific region
Place of Presentation
Siem Reap, Cambodia
Year and Date
2010-03-23
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