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Specific gene expression and its functional analysis in the maturing rat testis.

Research Project

Project/Area Number 21591315
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Pediatrics
Research InstitutionUniversity of Yamanashi

Principal Investigator

OHYAMA Kenji  山梨大学, 医学工学総合研究部, 医学研究員 (80051861)

Co-Investigator(Kenkyū-buntansha) YAMANO Yoshiaki  鳥取大学, 農学部, 教授 (00182593)
OHTA Masanori  山梨大学, 医学工学総合研究部, 医学研究員 (80233146)
SANO Tomoaki  山梨大学, 医学工学総合研究, 医学研究員 (80447705)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
KeywordsSpermatogenesis / Testis / azospermia / sexual maturation / Ttc29 gene / 精子形成 / Ttc29(NYD-SP14) / 抗ミュラー管ホルモン / AMH受容体 / アンドロゲン受容体 / 下垂体摘出ラット / 性成熟 / ゴナドトロピン / 下垂体摘出 / NYD-sp 14 / gonadotropin / testosterone / 癌精巣抗原
Research Abstract

Spermatogenesis is a complex process, and many genes are systematically expressed. We screened 4 genes in maturing rat testis using differential display method. Hrasls5 gene product might function as a tumor suppressor as well as in spermatogenesis, as deduced from its amino acid sequence. RGD1303144 gene was expressed dominantly in spermatocytes. Colon cancer autoantigen gene, Sdccag8, was also localized to the spermatocytes in 7-week-old rat testis, and its protein was detected in the spermatids. Sdccag8 may have a role in the development from spermatocyte to spermatid. In situ hybridization revealed that Ttc29 was expressed primarily in spermatocytes. Ttc29 expression was up-regulated by treatment with FSH. Ttc29 encodes axonemal dynein, which is a component of sperm flagella. These data indicate that axonemal dynein expression starts in spermatocytes and is regulated by FSH

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (29 results)

All 2012 2011 2010 2009 2008 Other

All Journal Article (12 results) (of which Peer Reviewed: 12 results) Presentation (16 results) Remarks (1 results)

  • [Journal Article] Regulation of rat tetratricopeptide repeat domain 29 gene expression by follicle stimulating hormone2012

    • Author(s)
      Ohta M, Ohyama K, AsanoA, Yokota S, Khalid AM, Yamano Y
    • Journal Title

      Biosci Biotechnol Biochem

      Volume: (in press)

    • NAID

      10031066189

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] 日本の性分化疾患の実情2011

    • Author(s)
      大山建司
    • Journal Title

      日本小児科学会雑誌

      Volume: 115 Pages: 1-4

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Aromatase excess syndrome : Identification of cryptic duplications and deletions leading to gain of function of Cyp19Al and assessment of phenotypic determinants2011

    • Author(s)
      Fukami A, et al
    • Journal Title

      J Clin Endocrinol Metab

      Volume: 96

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression of the centrosomal col on cancer autoantigen gene during spe rmatogenesis in the maturing rat test is2010

    • Author(s)
      Kamio T, Asano A, Hosaka YZ, Khalid A M, Yokota S, Ohta M, Ohyama K, Yamano Y
    • Journal Title

      Biotechnol Biochem

      Volume: 74 Pages: 1466-1469

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Expression of the centrosomal colon cancer autoantigen gene during spermatogenesis in the maturing testis2010

    • Author(s)
      Kamio T, et al
    • Journal Title

      Biosci Biotech Biochem

      Volume: 74

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression of the centrosomal colon cancer autoantigen gene during spermatogenesis in maturing rat testis.2010

    • Author(s)
      Kamio T.
    • Journal Title

      Biosci Biotech Biochem

      Volume: 74

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Exposure to exogenous estrogen through intake of commercial milk from pregnant cows.2010

    • Author(s)
      Maruyama K
    • Journal Title

      Pediatr International

      Volume: 52 Pages: 33-38

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Late-onset circulatory dysfunction after thyroid hormone treatment in an extremely low birth weight infant.2010

    • Author(s)
      Yagasaki H.
    • Journal Title

      J Pediatr Endocrinol Metab

      Volume: 23 Pages: 153-158

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression of rat sperm flagellum-movement associated protein genes under 2, 3, 7, 8-tetrachl orodibenzo-p-dioxin treatment2009

    • Author(s)
      Yamano Y, Asano A, Ohta M, Hirata S, Shoda T, Ohyama K
    • Journal Title

      Biotec hnol Biochem

      Volume: 73 Pages: 946-949

    • NAID

      10027540169

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Expression of rat sperm flagellum-movement associated protein genes under 2, 3, 7, 8 tetrachlorodibenzo-p-dioxin treatment.2009

    • Author(s)
      Yamano Y,
    • Journal Title

      Biosci Biotech Biochem 73

    • NAID

      10027540169

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression of stanniocalcin-1 in gastrointestinal tracts of neonatal and matured rats.2009

    • Author(s)
      Kobayashi R,
    • Journal Title

      Biochem Biophys Res Commun 389

      Pages: 478-483

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression of the Ha-ras suppressor family member 5 gene in the maturing rat testis2008

    • Author(s)
      Yamano Y, Asano A, Ohyama K, Ohta M, Nishio R, Morishima I
    • Journal Title

      Biosci Biotechnol Biochem

      Volume: 72 Pages: 1360-1363

    • NAID

      10027528126

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Presentation] ラット精巣における結腸癌抗原遺伝子の発現調節について2011

    • Author(s)
      太田正法
    • Organizer
      日本小児内分泌学会
    • Place of Presentation
      大宮スニックシティ(大宮)
    • Year and Date
      2011-10-07
    • Related Report
      2011 Annual Research Report
  • [Presentation] 性の分化と成熟2011

    • Author(s)
      大山建司
    • Organizer
      北海道内分泌代謝フォーラム
    • Place of Presentation
      札幌コンベンションセンター(札幌)
    • Year and Date
      2011-10-01
    • Related Report
      2011 Annual Research Report
  • [Presentation] 性の分化と成熟2011

    • Author(s)
      大山建司
    • Organizer
      九州小児内分泌学会
    • Place of Presentation
      福岡国際センター(博多)
    • Year and Date
      2011-07-11
    • Related Report
      2011 Annual Research Report
  • [Presentation] エストロゲンによる間脳下垂体性腺系抑制機構の解明2011

    • Author(s)
      深見真紀
    • Organizer
      日本内分泌学会学術集会
    • Place of Presentation
      神戸国際会議場(神戸)
    • Year and Date
      2011-04-22
    • Related Report
      2011 Annual Research Report
  • [Presentation] アロマターゼ過剰症兄弟例の脂肪細胞におけるアロマターゼ発現および活性の検討2011

    • Author(s)
      佐野友昭
    • Organizer
      日本内分泌学会学術集会
    • Place of Presentation
      神戸国際会議場(神戸)
    • Year and Date
      2011-04-22
    • Related Report
      2011 Annual Research Report
  • [Presentation] 精子鞭毛運動関連遺伝子の発現制御に関する検討2011

    • Author(s)
      太田正法
    • Organizer
      日本内分泌学会学術集会
    • Place of Presentation
      神戸国際会議場(神戸)
    • Year and Date
      2011-04-22
    • Related Report
      2011 Annual Research Report
  • [Presentation] 日本における性分化疾患の実態2010

    • Author(s)
      大山建司
    • Organizer
      第44回日本小児内分泌学会
    • Place of Presentation
      大阪
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] Expression of TPR domain con taining NYD-sp14 gene in the maturing rat testis2010

    • Author(s)
      Ohta M
    • Organizer
      14th International Congr ess of Endocrinology
    • Place of Presentation
      Kyoto
    • Related Report
      2011 Final Research Report
  • [Presentation] 精子形成期のラット精巣特異的に発現する結腸癌抗原に関する検討2010

    • Author(s)
      太田正法
    • Organizer
      第15回日本生殖内分泌学会
    • Place of Presentation
      大阪
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] Expression analysis of CT antigen gene in maturing rat testis2010

    • Author(s)
      Yamano Y
    • Organizer
      第33回日本分子生物学会
    • Place of Presentation
      神戸
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] Expression of TPR domain containing NYD-sp14 gene in the maturing rat testis2010

    • Author(s)
      Ohta M
    • Organizer
      14th International Congress of Endocrinology
    • Place of Presentation
      Kyoto
    • Related Report
      2010 Annual Research Report
  • [Presentation] 精子形成期ラット精巣における精子鞭毛関連遺伝子の発現解析2009

    • Author(s)
      山野好章
    • Organizer
      日本第32回分子生物学会
    • Place of Presentation
      横浜
    • Year and Date
      2009-11-02
    • Related Report
      2011 Final Research Report
  • [Presentation] 精子形成期ラット精巣における精子鞭毛関連遺伝子の発現解析2009

    • Author(s)
      山野好章
    • Organizer
      日本分子生物学会
    • Place of Presentation
      横浜
    • Year and Date
      2009-11-02
    • Related Report
      2009 Annual Research Report
  • [Presentation] 精子形成サイクルにおける鞭毛運動関連遺伝子に関する検討2009

    • Author(s)
      太田正法
    • Organizer
      第43回日本小児内分泌学会
    • Place of Presentation
      宇都宮
    • Year and Date
      2009-10-03
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Presentation] ラット精巣における結腸ガン抗原遺伝子の発現解析2009

    • Author(s)
      神尾高志
    • Organizer
      第148回日本獣医学会
    • Place of Presentation
      鳥取
    • Year and Date
      2009-09-05
    • Related Report
      2009 Annual Research Report
  • [Presentation] 精子形成に関与する遺伝子発現に対するゴナドトロピンの影響について2009

    • Author(s)
      太田正法
    • Organizer
      第82回日本内分泌学会
    • Place of Presentation
      前橋
    • Year and Date
      2009-04-23
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Remarks]

    • URL

      http://erdb.yamanashi.ac.jp/rdb/A_DispInfo.Scholar/3_89/41A65B722005B9C6.html

    • Related Report
      2009 Annual Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

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