Epigenetic control of gene expression associated with adult diseases due to fetal nutritional factors

• Project/Area Number: 21591409
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Embryonic/Neonatal medicine
• Research Institution: Tohoku University
• Principal Investigator: MUROTSUKI Jun 東北大学, 大学院・医学系研究科, 非常勤講師 (50239555)
• Co-Investigator(Kenkyū-buntansha): SATO Kazuyo 東北大学, 大学院・医学系研究科, 非常勤講師 (70375020) ; ARAKI Yoshihiko 順天堂大学, 環境医学研究所, 准教授 (70250933)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: DOHaD仮説 / 低タンパク食 / メチル化 / エピジェネティクス / 妊娠マウス / DOHaD / 2013/10/04 / SPHK-1 / epigenetics / metabolic disease / 胎児発育遅延 / 成人病 / 子宮内環境 / マウス

Research Abstract

One likely mechanism of the developmental origins hypothesis is via early nutritional influences on epigenetic gene modification consisting of the presence of a methyl group on the carbon 5 of a cytosine residue. We have tested the hypothesis that maternal low-protein diet altered epigenetic regulation of specific gene of the offspring. Hepatic Oct-4 or Sphk-1 methylation pattern of the genomic DNA is specific in low-protein diet group. Aberrant Oct-4 and Sphk-1 gene expression may cause perturbations in cell differentiation. We suggest that the epigenetic mechanism consisting of DNA methylation underlies the fetal programming theory.

Research Products

• [Journal Article] Association of Women's Birth Weight with Their Blood Pressure during Pregnancy and with the Body Size of Their Babies (2011)
  • Author(s): Tanabe K, Murotsuki J, et al
  • Journal Title: The Tohoku Journal of Experimental Medicine Volume: 224 Issue: 4 Pages: 287-292
  • DOI: 10.1620/tjem.224.287
  • NAID: 10029508069
  • ISSN: 0040-8727, 1349-3329
  • Peer Reviewed
• [Journal Article] Ultrasonic diagnosis of fetal bone and small parts (2011)
  • Author(s): Sekiya T, Murotsuki J, et al
  • Journal Title: Donald School Journal of Ultrasound in Obstetrics and Gynecology Volume: 5 Issue: 1 Pages: 45-55
  • DOI: 10.5005/jp-journals-10009-1176
  • Peer Reviewed
• [Journal Article] Antenatal three-dimensional sonographic features of Roberts syndrome (2011)
  • Author(s): Dulnuan DJ, Murotsuki J, et al
  • Journal Title: Arch Gynecol Obstet Volume: 284 Issue: 1 Pages: 241-244
  • DOI: 10.1007/s00404-011-1910-1
  • Peer Reviewed
• [Journal Article] 四肢骨格の超音波像 (2011)
  • Author(s): 室月淳
  • Journal Title: 臨床婦人科産科 Volume: 64 Pages: 612-617
• [Journal Article] The outcome and prognostic factors of twin. twin transfusion syndrome following fetoscopic laser surgery (2010)
  • Author(s): Haruhiko Sago, Satoshi Hayashi Jun Murotsuki, et al
  • Journal Title: Prenatal Diagnosis Volume: 30 Pages: 1185-1191
  • Peer Reviewed
• [Journal Article] 双胎間輸血症候群に対するレーザー手術による新生児合併症 (2010)
  • Author(s): 室月淳, 左合治彦, 林聡, 加藤有美, 難波由喜子, 伊藤裕司, 村越毅, 他
  • Journal Title: 日本周産期・新生児医学会誌 Volume: 46 Pages: 14-16
  • Peer Reviewed
• [Journal Article] The outcome and prognostic factors of twin-twin transfusion syndrome following fetoscopic laser surgery (2010)
  • Author(s): Haruhiko Sago, Satoshi Hayashi, Jun Murotsuki, et al
  • Journal Title: Prenatal Diagnosis Volume: 30 Pages: 1185-1191
  • Peer Reviewed
• [Journal Article] 胎児の異常所見と疾患 (2009)
  • Author(s): 今井紀昭, 室月淳
  • Journal Title: ペリネイタルケア Volume: 28 Pages: 706-710
• [Journal Article] 胎児の異常所見と疾患 (2009)
  • Author(s): 今井紀昭, 室月淳
  • Journal Title: ペリネイタルケア 28 Pages: 706-710
• [Presentation] 胎児心疾患に対するインターベンション-ISPD2010報告 (2010)
  • Author(s): 室月淳
  • Organizer: 第8回日本胎児治療学会
  • Place of Presentation: 埼玉県川越市
  • Year and Date: 2010-11-12
• [Presentation] 一絨毛膜双胎に生じる病態についての新しい概念と胎児治療 (2009)
  • Author(s): 室月淳
  • Organizer: 第57回日産婦北日本連合地方部会(特別講演)
  • Place of Presentation: 札幌
  • Year and Date: 2009-08-29
• [Presentation] 一絨毛膜双胎に生じる病態についての新しい概念と胎児治療 (2009)
  • Author(s): 室月淳
  • Organizer: 第57回日産婦北日本連合地方部会特別講演
  • Place of Presentation: 札幌
  • Year and Date: 2009-08-29
• [Remarks]
  • URL: http://www.ob-gy.med.tohoku.ac.jp/laboratory/j-murotsuki.html