Exploration of pathogenetic mechanism for hair diseases through dermal fibroblasts

• Project/Area Number: 21591427
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Dermatology
• Research Institution: Hirosaki University
• Principal Investigator: NAKANO Hajime 弘前大学, 大学院・医学研究科, 准教授 (90281922)
• Co-Investigator(Kenkyū-buntansha): MATSUZAKI Yasushi 弘前大学, 医学部附属病院, 講師 (50322946) ; SAWAMUERA Daisuke 弘前大学, 大学院・医学研究科, 教授 (60196334)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: ヒロサキヘアレスラット / 乏毛 / 繊維芽細胞 / 細胞周期 / 脱毛疾患 / 線維芽細胞

Research Abstract

Primary culture of the dermal fibroblasts derived from Hirosaki hairless rat (HHR-DF) develops multinuclear large fibroblasts after multiple culture passages. These cells have low proliferation ability. Several growth stimuli including calf serum, TGF-beta, and bFGF failed to promote cell proliferation of HHR-DF. Artificial skin defect on the dorsal skin of HHR showed retarded wound healing. These results suggest that HHR-DF has an impaired sensitivity to growth stimuli.

Research Products

• [Journal Article] Buschke-Ollendorff syndrome associated with hypertrophic scar formation : a possible role for LEMD3 mutation (2012)
  • Author(s): Korekawa A, Nakano H, Toyomaki Y, Takiyoshi N, Rokunohe D, Akasaka E, Nakajima K, Sawamura D
  • Journal Title: Br J Dermatol Volume: 166 Issue: 4 Pages: 900-903
  • DOI: 10.1111/j.1365-2133.2011.10691.x
  • Peer Reviewed
• [Journal Article] Diffuse and focal palmoplantar keratoderma can be caused by a keratin 6c mutation (2011)
  • Author(s): Akasaka E, Nakano H, Nakano A, Toyomaki Y, Takiyoshi N, Rokunohe D, Nishikawa Y, Korekawa A, Matsuzaki Y, Mitsuhashi Y, Sawamura D
  • Journal Title: Br J Dermatol Volume: 165(6) Issue: 6 Pages: 1290-1292
  • DOI: 10.1111/j.1365-2133.2011.10552.x
  • Peer Reviewed
• [Journal Article] Vorner type palmoplantar keratoderma : novel KRT9 mutation associated with knuckle pad-like lesions and recurrent mutation causing digital mutilation (2011)
  • Author(s): Umegaki N, Nakano H, Tamai K, Mitsuhashi Y, Akasaka E, Sawamura D, Katayama I
  • Journal Title: Br J Dermatol Volume: 165(1) Issue: 1 Pages: 199-201
  • DOI: 10.1111/j.1365-2133.2011.10317.x
  • Peer Reviewed
• [Journal Article] Vorner type palmoplantar keratoderma : novel KRT9 mutation associated with knuckle pad-like lesions and recurrent mutation causing digital mutilation (2011)
  • Author(s): Umegaki, et al.
  • Journal Title: Br J Dermatol Volume: 165 Pages: 199-201
  • Peer Reviewed
• [Journal Article] Leukemia cutis is an early sign of blast transformation that may mimic a common skin disease : sycosis-like eruptions in chronic myelomonocytic leukemia (2010)
  • Author(s): Takeuchi S, Akasaka E, Rokunohe D, Kayo SJ, Matsuzaki Y, Ooshima H, Nakano H, Sawamura D
  • Journal Title: J Dermatol Volume: 37 Issue: 10 Pages: 916-918
  • DOI: 10.1111/j.1346-8138.2010.00901.x
  • NAID: 10027572457
  • Peer Reviewed
• [Journal Article] Leukemia cutis is an early sign of blast transformation that may mimic a common skin disease : sycosis-like eruptions in chronic myelomonocvtic leukemia. (2010)
  • Author(s): Sonoko Takeuchi, et al.
  • Journal Title: J Dermatol Volume: 37 Pages: 916-918
  • Peer Reviewed
• [Journal Article] Characterization of Hair Follicles in Hirosaki Hairless Rats with Deletion of Basic Hair Keratin Genes. Enlarged Medulla, Loss of Cuticle and Long Catagen (2009)
  • Author(s): Akita M, Nanashima N, Yamada T, Nakano H, Shimizu T, Fan Y, Tsuchida S
  • Journal Title: Hirosaki Medical Journal Volume: 60 Pages: 1-11
  • NAID: 110007079174
  • URL: http://ci.nii.ac.jp/naid/110007079174
  • Peer Reviewed
• [Journal Article] Characterization of Hair Follicles in Hirosaki Hairless Rats with Deletion of Basic Hair Keratin Genes. Enlarged Medulla, Loss of Cuticle and Long Catagen (2009)
  • Author(s): Miki Akita
  • Journal Title: Hirosaki Medical Journal 60 Pages: 1-11
  • Peer Reviewed
• [Presentation] ヘアケラチン遺伝子の欠損がある弘前ヘアレスラット(HHR)の毛構造の変化、毛周期の変化 (2009)
  • Author(s): 秋田美季
  • Organizer: 第108回日本皮膚科学会総会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2009-04-25
• [Presentation] ヘアケラチン遺伝子の欠損がある弘前ヘアレスラットの毛構造の変化、毛周期の変化 (2009)
  • Author(s): 秋田美季
  • Organizer: 第108回日本皮膚科学会総会
  • Place of Presentation: 福岡市
  • Year and Date: 2009-04-25
• [Book] 顔の皮膚病最前線(宮地良樹編集) (2010)
  • Author(s): 中野創
  • Total Pages: 4
  • Publisher: メディカルレビュー社