The efficacy of anti-Aβoligomer antibody to the toxicity of Aβto neuronal cells

• Project/Area Number: 21591524
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Psychiatric science
• Research Institution: University of Miyazaki
• Principal Investigator: HAYASHI Yoshihito 宮崎大学, 医学部, 講師 (20295223)
• Co-Investigator(Kenkyū-buntansha): EBIHARA Kousuke 宮崎大学, 医学部, 助教 (20510720) ; HUNAHASHI Hideki 宮崎大学, 医学部, 助教 (10404435)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: アルツハイマー型認知症 / βアミロイド / オリゴマー / 神経細胞保護

Research Abstract

The most popular type of dementia is Alzheimer's disease(AD). The cause of AD is thought that amyloid beta protein(Aβ) cleaved from amyloid precursor protein(APP) which is expressing on the neuronal cell membranes has neurotoxicity and induce neuronal cell death. Recently some researchers found the soluble oligomer form had toxicity to the synapses and induce cognitive decline. We thought that anti-oligomer antibody has neuroprotective effect. We investigated the mechanisms of neuroprotective effect of anti-oligomer antibody against neuronal cells from Aβtoxicity. We investigated whether or not anti-oligomer antibody reduce the amount of free radical formation when Aβaggregate themselves. The amount of free radical formation was reduced compared with control solution. The viability of PC12 cells was higher when culture medium was added anti-oligomer antibody with Aβ. We found some changes of amount of NMDA receptor in PC12 when added Aβto culture medium with anti-oligomer antibody. We did not confirm whether the effect of anti-oligomer antibody against NMDA receptor was direct or indirect effect. We thought that there are some relations between two properties.

Research Products

• [Journal Article] 認知症患者の行動・心理症状(BPSD)に対する抑肝散長期投与の安産性および有効性の検討 (2012)
  • Author(s): 岡原一徳、石田康、林要人、土屋利紀
  • Journal Title: Dementia Japan Volume: 26 Pages: 196-205
  • Peer Reviewed
• [Journal Article] アルツハイマー型認知症の周辺症状(BPSD)に対する抑肝散の効果-日常診療下における検討:入院患者における部分集団解析- (2010)
  • Author(s): 林要人, 石田康, 井上輝彦, 宇田川充隆, 竹内康三, 木上幸一郎, 二宮嘉正, 河野次郎, 鮫島哲郎, 川原岳, 後藤勇, 首藤謙二, 倉山茂樹, 中村純五, 岡原一徳, 三山吉夫
  • Journal Title: Geriatric Medicine(老年医学) Volume: 48巻6号 Pages: 831-838
  • Peer Reviewed
• [Journal Article] Treatment of behavioral and psychological symptoms of Alzheimer-type dementia with Yokukansan in clinical practice (2010)
  • Author(s): Hayashi Y, Ishida Y, Inoue T, Udagawa M, Takeuchi K, Yoshimuta H, Kiue K, Ninomiya Y, Kawano J, Sameshima T, Kawahara T, Goto I, Shudo K, Kurayama S, Nakamura J, Okahara K, Mitsuyama Y
  • Journal Title: Prog Neuropsychopharmacol Biol Psychiatry Volume: 34-3 Pages: 541-5
  • Peer Reviewed
• [Journal Article] Effects of Yokukansan on behavioral and psychological symptoms of dementia in regular treatment for Alzheimer's disease (2010)
  • Author(s): Okahara K, Ishida Y, Hayashi Y, Inoue T, Tsuruta K, Takeuchi K, Yoshimuta H, Kiue K, Ninomiya Y, Kawano J, Yoshida K, Noda S, Tomita S, Fujimoto M, Hosomi J, Mitsuyama Y
  • Journal Title: Prog Neuropsychopharmacol Biol Psychiatry. Volume: 34-3 Pages: 532-6
  • Peer Reviewed
• [Journal Article] Treatment of behavioral and psychological symptoms of Alzheimer-type dementia with Yokukansan in clinical practice. (2010)
  • Author(s): Yoshihito Hayashi, et al
  • Journal Title: Prog Neuropsychopharmacol Biol Psychiatry Volume: 34 Pages: 541-545
  • Peer Reviewed
• [Journal Article] アルツハイマー型認知症の周辺症状(BPSD)に対する抑肝散の効果 (2010)
  • Author(s): 林要人, 他
  • Journal Title: Geriatric Medicine(老年医学) Volume: 48 Pages: 831-838
  • Peer Reviewed
• [Journal Article] アルツハイマー型認知症患者の行動および心理症状に対する抑肝散の効果 (2009)
  • Author(s): 林要人, 石田康, 岡原一徳, 三山吉夫
  • Journal Title: 脳21 Volume: 12巻4号 Pages: 452-455
  • Peer Reviewed
• [Journal Article] Analgesic effect of milnacipran is associated with c-Fos expression in the anterior cingulate cortex in the rat neuropathic pain model (2009)
  • Author(s): Takeda R, Watanabe Y, Ikeda T, Abe H, Ebihara K, Matsuo H, Nonaka H, Hashiguchi H, Nishimori T, Ishida Y
  • Journal Title: Neurosci Res Volume: 64-4 Pages: 380-384
  • Peer Reviewed
• [Journal Article] アルツハイマー型認知症患者の行動および心理症状に対する抑肝散の効果-日常診療下における検討- (2009)
  • Author(s): 林要人, 他
  • Journal Title: 脳21 12 Pages: 452-455
  • Peer Reviewed
• [Presentation] An open label study to ascertain the effect of a traditional Japanese medicine yokukansan, short-term treatment on the BPSD in patients with AD (2011)
  • Author(s): Yoshihito Hayashi
  • Organizer: アメリカ精神医学会
  • Place of Presentation: アメリカ合衆国ハワイ
  • Year and Date: 2011-05-16
• [Presentation] An open label study to ascertain the effect of a traditional Japanese medicine yokukansan, short-term treatment on the BPSD in patients with AD (2011)
  • Author(s): Hayashi Y, Ishida Y, Okahara K, Mitsuyama Y
  • Organizer: アメリカ精神医学会
  • Place of Presentation: ハワイ
• [Presentation] Effect of aripiprazole on methamphetamine-induced disruption of latent inhibition in rats (2010)
  • Author(s): Matsuo H, Abe H, Ebihara K, Takeda R, Nishimori T, Ishida Y
  • Organizer: 第40回北米神経科学学会
  • Place of Presentation: サンディゴ
• [Presentation] A randomized, controlled comparative open label study of a traditional Japanese medicine, yokukansan, on the behavioral and psychological symptoms of dementia in patients with Alzheimer's disease (2009)
  • Author(s): Yoshihito Hayashi, Y Mitsuyama, Y Ishida
  • Organizer: International Psychogeriatric Association 14^<th> International Congress
  • Place of Presentation: カナダ モントリオール国際会議場
  • Year and Date: 2009-09-04
• [Presentation] A multicenter open label study to investigate the effect of a traditional Japanese medicine, yokukansan, on the behavioral and psychological symptoms of dementia in patients with Alzheimer's disease (2009)
  • Author(s): Yoshihito Hayashi, Y Mitsuyama, Y Ishida
  • Organizer: International Psychogeriatric Association 14^<th> International Congress
  • Place of Presentation: カナダ モントリオール国際会議場
  • Year and Date: 2009-09-04
• [Presentation] A multicenter open label study to investigate the effect of a traditional Japanese medicine, yokukansan, on the behavioral and psychological symptoms of dementia in patients with Alzheimer's disease (2009)
  • Author(s): Hayashi Y, Ishida Y, Okahara K, Mitsuyama Y
  • Organizer: 国際老年精神医学会
  • Place of Presentation: カナダモントリオール
• [Presentation] A randomized, controlled comparative open label study of a traditional Japanese medicine, yokukansan, on the behavioral and psychological symptoms of dementia in patients with Alzheimer's disease (2009)
  • Author(s): Hayashi Y, Ishida Y, Okahara K, Mitsuyama Y
  • Organizer: 国際老年精神医学会
  • Place of Presentation: カナダモントリオール
• [Presentation] オレキシンAによる脳内自己刺激閾値の上昇はCRF受容体阻害によって抑制される (2009)
  • Author(s): 名古屋畑敏道, 蛯原功介, 伊達紫, 石田康, 中原大一郎
  • Organizer: 第32回日本神経科学大会