Establishment of a new evaluation method of post-transplant immune tolerance by donor-specific regulatory T cell analysis

• Project/Area Number: 21591634
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General surgery
• Research Institution: Nagoya University
• Principal Investigator: HANEDA Masataka 名古屋大学, 大学院・医学系研究科, 講座講師 (50436995)
• Co-Investigator(Kenkyū-buntansha): KOBAYASHI Takaaki 名古屋大学, 医学系研究科, 講座教授 (70314010) ; IWASAKI Kenta 名古屋大学, 医学系研究科, 講座助教 (10508881)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: ドナー特異性 / allo reactive T細胞 / 制御性T細胞 / Anergy / clonal deletion / ELISpot assay / 移植免疫 / トレランス / anergy / Clonal deletion / TCR repertoire解析 / T cell cloning

Research Abstract

We performed analysis by using the improved ELISpot assay for monitoring the donor specific allo reactive T cells. The numbers of allo reactive T cells were not altered in one year after kidney transplantation. We also analyzed about the relationship between rejection and Treg cells number in the peripheral blood. Once the expression of Foxp3 is lowered after transplantation, one year after the transplant was recovered up to the value before transplantation. Acute rejection does not occur more than one year after, because Treg, rather than clonal deletion and anergy, may be due to immune regulation.

Research Products

• [Journal Article] Comparative Study on Signal Transduction in Endothelial Cells After Anti-A/B and Human Leukocyte Antigen Antibody Reaction : Implication of Accommodation (2012)
  • Author(s): Iwasaki K, Miwa Y, Ogawa H, Yazaki S, Iwamoto M, Furusawa T, Onishi A, Kuzuya T, Haneda M, Watarai Y, Uchida K, Kobayashi T.
  • Journal Title: Transplantation Volume: 93(4) Pages: 390-397
  • Peer Reviewed
• [Journal Article] Comparative Study on Signal Transduction in Endothelial Cells After Anti-A/B and Human Leukocyte Antigen Antibody Reaction : Implication of Accommodation (2012)
  • Author(s): 岩崎研太、三輪裕子、羽根田正隆、小林孝彰
  • Journal Title: Transplantation Volume: 93 Pages: 390-397
  • Peer Reviewed
• [Journal Article] Clinical significance of regulatory T-cell-related gene expression in peripheral blood after renaltrans plantation (2011)
  • Author(s): Iwase H, Kobayashi T, Kodera Y, Miwa Y, Kuzuya T, Iwasaki K, Haneda M, Katayama A, Takeda A, Morozumi K, Watarai Y, Uchida K, Nakao A.
  • Journal Title: Transplantation Volume: 91(2) Pages: 191-8
  • Peer Reviewed
• [Journal Article] Significance of HLA class I antibody-induced antioxidant gene expression for end othe lial cell protection against complement attack (2010)
  • Author(s): Iwasaki K, Miwa Y, Haneda M, Uchida K, Nakao A, Kobayashi T.
  • Journal Title: Biochem Biophys Res Commun Volume: 391(2) Pages: 1210-5
  • Peer Reviewed
• [Journal Article] Potential value of human throm bomodul in and DAF expression for coagulation control in pig-to-human xenotrans plantation (2010)
  • Author(s): Miwa Y, Yamamoto K, Onishi A, Iwamoto M, Yazaki S, Haneda M, Iwasaki K, Liu D, Ogawa H, Nagasaka T, Uchida K, Nakao A, Kadomatsu K, Kobayashi T.
  • Journal Title: Xenotransplantation Volume: 17(1) Pages: 26-37
  • Peer Reviewed
• [Journal Article] Potential value of human thrombomodulin and DAF expression for coagulation control in pig-to-human xenotransplantation. (2010)
  • Author(s): 三輪裕子、羽根田正隆、岩崎研太、小林孝彰
  • Journal Title: Xenotransplantation. Volume: 17 Pages: 26-37
  • Peer Reviewed
• [Journal Article] Clinical significance of regulatory T-cell-related gene expression in peripheral blood after renal transplantation. (2010)
  • Author(s): 岩瀬勇人、岩崎研太、羽根田正隆、小林孝彰
  • Journal Title: Transplantation Volume: 91 Pages: 191-198
  • Peer Reviewed
• [Journal Article] Significance of HLA class I antibody-induced antioxidant gene expression for endothelial cell protection against complement attack. (2010)
  • Author(s): 岩崎研太、羽根田正隆、小林孝彰
  • Journal Title: Biochemical and Biophysical Research Communications 391 Pages: 1210-1215
  • Peer Reviewed
• [Journal Article] Stimulation index for PCNA mRNA in peripheral blood as immune function monitoring after renaltrans plantation (2009)
  • Author(s): Niwa M, Miwa Y, Kuzuya T, Iwasaki K, Haneda M, Ueki T, Katayama A, Hiramitsu T, Goto N, Nagasaka T, Watarai Y, Uchida K, Nakao A, Kobayashi T.
  • Journal Title: Transplantation Volume: 87(9) Pages: 1411-4
  • Peer Reviewed
• [Journal Article] Pretransplant pharmacodynamic analysis of immuno suppressive agents using CFSE-basedT-cell proliferation assay (2009)
  • Author(s): Kurata Y, Kato M, Kuzuya T, Miwa Y, Iwasaki K, Haneda M, Amioka K, Watarai Y, Uchida K, Nakao A, Kobayashi T.
  • Journal Title: Clin Pharmacol Ther Volume: 86(3) Pages: 285-9
  • Peer Reviewed
• [Journal Article] Stimulation index for PCNA mRNA in peripheral blood as immune function monitoring after renal transplantation. (2009)
  • Author(s): 丹羽操、岩崎研太、羽根田正隆, 小林孝彰
  • Journal Title: Transplantation 87 Pages: 1411-1414
  • Peer Reviewed
• [Journal Article] Pretransplant pharmacodynamic analysis of immunosuppressive agents using CFSE-based T-cell proliferation assay. (2009)
  • Author(s): 倉田洋子、岩崎研太、羽根田正隆, 小林孝彰
  • Journal Title: Clinical Pharmacology & Therapeutics 86 Pages: 285-289
  • Peer Reviewed
• [Presentation] 免疫抑制薬の抗体産生抑制効果に対する薬力学解析の試み (2011)
  • Author(s): 大脇麻未、羽根田正隆、倉田洋子、葛谷孝文、網岡克雄、山田清文、岩崎研太、小林孝彰
  • Organizer: 第47回日本移植学会
  • Place of Presentation: 仙台国際センター(宮城県)
  • Year and Date: 2011-10-05
• [Presentation] 免疫抑制薬の抗体産生抑制効果に対する薬力学解析の試み (2011)
  • Author(s): 大脇麻未・羽根田正隆・小林孝彰
  • Organizer: 日本移植学会
  • Place of Presentation: 仙台国際センター(宮城県)
  • Year and Date: 2011-10-05
• [Presentation] 腎移植後の免疫モニタリングとしての末梢血Foxp3mRNA定量の意義 (2010)
  • Author(s): 岩瀬勇人、小林孝彰、岩崎研太、羽根田正隆、片山昭男、武田朝美、両角國男、渡井至彦、打田和治、小寺泰弘、中尾昭公
  • Organizer: 第46回日本移植学会
  • Place of Presentation: みやこめっせ(京都府)
  • Year and Date: 2010-10-22
• [Presentation] ELIspot assayを用いた腎臓移植後のドナー特異的allo reactive T細胞の解析 (2010)
  • Author(s): 羽根田正隆、岩崎研太、小林孝彰
  • Organizer: 第46回日本移植学会総会
  • Place of Presentation: 京都
  • Year and Date: 2010-10-21
• [Presentation] ELISpot assayを用いた腎臓移植後のドナー特異的allo reactive T細胞の解析 (2010)
  • Author(s): 羽根田正隆、岩崎研太、小林孝彰、葛谷孝文、渡井至彦、武田朝美、両角國男、打田和治
  • Organizer: 第46回日本移植学会
  • Place of Presentation: みやこめっせ(京都府)
  • Year and Date: 2010-10-20
• [Presentation] 腎移植後の末梢血Foxp3mRNAモニタリングの臨床意義 (2010)
  • Author(s): 岩瀬勇人、小林孝彰、岩崎研太、羽根田正隆、片山昭男、武田朝美、渡井至彦、打田和治、小寺泰弘、中尾昭公
  • Organizer: 第110回日本外科学会定期学術集会
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2010-04-08
• [Presentation] Potential Value of Foxp3 and Regulatory T cell-Related Gene Expression Monitoring in Peripheral Blood After Renal Transplantation International Congress of The Transplantation Society (2010)
  • Author(s): Iwase, H.; Kobayashi, T.; Iwasaki, K.; Haneda, M.; Katayama, A.; Takeda, A.; Morozumi, K.; Watarai, Y.; Uchida, K.; Kodera, Y.; Nakao, A
  • Organizer: Vancouver(Canada)
• [Presentation] Im mune Function Monitoring of PCNA and P21 m RNA Expression in Stimulated Peripheral Blood after Renal Transplantation (2010)
  • Author(s): Niwa, M.; Miwa, Y.; Haneda, M.; Iwasaki, K.; Katayama, A.; Hiramitsu, T.; Goto, N.; Nagasaka, T.; Watarai, Y.; Uchida, K.; Kobayashi, T.
  • Organizer: AMERICAN TRANSPLANTCONGRESS 2010
  • Place of Presentation: SanDiego(USA)
• [Presentation] 腎移植における免疫寛容とFoxp3 (2009)
  • Author(s): 岩瀬勇人、小林孝彰、羽根田正隆、岩崎研太、片山昭男、渡井至彦、打田和治、中尾昭公
  • Organizer: 第45回日本移植学会
  • Place of Presentation: 京王プラザ(東京都)
  • Year and Date: 2009-09-17
• [Presentation] 腎臓移植後のドナー特異的allo reactiveT細胞の動態解析と病理所見の関連性について (2009)
  • Author(s): 羽根田正隆、岩崎研太、小林孝彰、葛谷孝文、渡井至彦、武田朝美、両角國男、打田和治
  • Organizer: 第45回日本移植学会
  • Place of Presentation: 京王プラザ(東京都)
  • Year and Date: 2009-09-17
• [Presentation] 腎臓移植後のドナー特異的allo reactive T細胞の動態解析と病理所見の関連性について (2009)
  • Author(s): 羽根田正隆、岩崎研太、小林孝彰
  • Organizer: 日本移植学会
  • Place of Presentation: 京王プラザ(東京都)
  • Year and Date: 2009-09-17
• [Presentation] Se miquan titative Analysis of Foxp3 (2009)
  • Author(s): Iwase, H.; Kobayashi, T.; Iwasaki, K.; Haneda, M.; Kuzuya, T.; Katayama, A.; Uchida, K.; Kodera, Y.; Nakao, A.
  • Organizer: ESOTParis(France)
• [Remarks]
  • URL: http://www.med.nagoya-u.ac.jp/tx-immunology/