Establishment of non-invasive diagnostic methods for allograft dysfunction and real-time monitoring of immunological status in liver transplant

• Project/Area Number: 21591637
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General surgery
• Research Institution: Osaka University
• Principal Investigator: MARUBASHI Shigeru 大阪大学, 医学系研究科, 助教 (20362725)
• Co-Investigator(Kenkyū-buntansha): NAGANO Hiroaki 大阪大学, 医学系研究科, 准教授 (10294050) ; KOBAYASHI Shougo 大阪大学, 医学系研究科, 助教 (30452436) ; TANEMURA Masahiro 大阪大学, 医学系研究科, 助教 (30379250) ; TAKEDA Yu 大阪大学, 医学系研究科, 助教 (90397696)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 肝移植 / 生体材料 / 拒絶反応 / DNAマイクロアレイ / プロテオーム解析 / 整体材料

Research Abstract

We investigated liver biopsy samples and bile samples in clinical liver transplant, applying molecular biological techniques. We found that biomarkers which were associated with acute cellular rejection after liver transplant in the two different OMICS levels ; DNA microarray and proteomic analyses. We then established the non-invasive method for diagnosing acute cellular rejection in liver transplant recipients.

Research Products

• [Journal Article] Significance of alanine aminopeptidase N(APN) in bile in the diagnosis of acute cellular rejection after liver transplantation (2011)
  • Author(s): Kim C, Aono S, Marubashi S, Wada H, Kobayashi S, Eguchi H, Takeda Y, Tanemura M, Okumura N, Takao T, Doki Y, Mori M, Nagano H.
  • Journal Title: Journal of Surgical Research
  • Peer Reviewed
• [Journal Article] Significance of alanine aminopeptidase N (APN) in bile in the diagnosis of acute cellular rejection after liver transplantation (2011)
  • Author(s): Kim S, Aono S, Marubashi S, 他
  • Journal Title: Journal of Surgical Research Volume: (In press)
  • Peer Reviewed
• [Journal Article] Guanylate-binding protein 2 mRNA in peripheral blood leukocytes of liver transplant recipients as a marker for acute cellular rejection (2010)
  • Author(s): Kobayashi S, Nagano H, Marubashi S, Hama N, Eguchi TA, Takeda Y, Tanemura M, Doki Y, Mori M.
  • Journal Title: Transplant International Volume: 23(4) Pages: 390-396
  • Peer Reviewed
• [Journal Article] Differential transcriptome patterns for acute cellular rejection in recipients with recurrent hepatitis C after liver transplantation (2009)
  • Author(s): Asaoka T, Kato T, Marubashi S, Dono K, Hama N, Takahashi H, Kobayashi S, Takeda Y, Takemasa I, Nagano H, Yoshida H, Ruiz P, Tzakis AG, Matsubara K, Monden M, Doki Y, Mori M.
  • Journal Title: Liver Transplantation Volume: 15(12) Pages: 1738-1749
  • Peer Reviewed
• [Journal Article] Differential transcriptome patterns for acute cellular rejection in recipients with recurrent hepatitis C after liver transplantation (2009)
  • Author(s): Asaoka, 他
  • Journal Title: Liver Transplantation 15(12) Pages: 1738-1749
  • Peer Reviewed
• [Journal Article] Guanylate-binding protein 2 mRNA in peripheral blood leukocytes of liver transplant recipients as a marker for acute cellular rejection (2009)
  • Author(s): Kobayashi, 他
  • Journal Title: Transplant International Nov 11(Epub)
  • Peer Reviewed
• [Presentation] iTRAQ法を用いた胆汁中蛋白質の網羅的発現解析の試み (2011)
  • Author(s): 畠野尚典、丸橋繁、他
  • Organizer: 第111回日本外科学会
  • Place of Presentation: 広島市
  • Year and Date: 2011-05-25
• [Presentation] iTRAQ法を用いた胆汁中蛋白質の網羅的発現解析の試み (2011)
  • Author(s): 畠野尚典、丸橋繁
  • Organizer: 第111回日本外科学会
  • Place of Presentation: 東京
  • Year and Date: 2011-05-25
• [Presentation] 臨床肝移植急性拒絶における末梢決IRFとGBP2発現の意義 (2010)
  • Author(s): 小林省吾、永野浩昭、丸橋繁、他
  • Organizer: 第28回日本肝移植研究会
  • Place of Presentation: 広島市
  • Year and Date: 2010-07-01
• [Presentation] 臨床肝移植急性拒絶における末梢決IRFとGBP2発現の意義 (2010)
  • Author(s): 小林省吾、永野浩昭、丸橋繁, 他
  • Organizer: 第28回 日本肝移植研究会
  • Place of Presentation: 広島市
  • Year and Date: 2010-07-01