Mechanism of premetastatic niche formation and promotion of metastasis by cancer mesenchymal cells

• Project/Area Number: 21591655
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General surgery
• Research Institution: Hokkaido University
• Principal Investigator: HIDA Yasuhiro 北海道大学, 北海道大学病院, 講師 (30399919)
• Co-Investigator(Kenkyū-buntansha): HIDA Kyoko 北海道大学, 大学院・歯学研究科, 特任准教授 (40399952) ; KONDO Satoshi 北海道大学, 大学院・医学研究科, 教授 (30215454)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 癌 / 転移 / 血管新生 / 血管内皮細胞 / ニッシェ / ニッシュ

Research Abstract

Promotion of lung metastasis by interaction between tumor and tumor endothelial cells
In this study, it was shown that tumor endothelial cells promote invasion and metastasis of tumor cells using cells labeled with fluorescein proteins. Tumor cells inoculated to mice with tumor endothelial cells circulated in peripheral blood and metastasized to lungs more than control conditions. The mechanism are enhanced migration of tumor cells towards tumor endothelial cells and transendothelial migration.

Research Products

• [Journal Article] CXCR7: A novel tumor endothelial marker in renal cell carcinoma (2012)
  • Author(s): Maishi N, Ohga N, Hida Y, Akiyama K, Shinohara N, Nonomura K, Shindoh M, Hida K. Kitayama K, Osawa T, Onodera Y
  • Journal Title: Pathol Int Volume: 65(5) Issue: 5 Pages: 309-17
  • DOI: 10.1111/j.1440-1827.2012.02792.x
  • NAID: 10031185206
  • Peer Reviewed
• [Journal Article] Tumor-Derived Microvesicles Induce Proangiogenic Phenotype in Endothelial Cells via Endocytosis (2012)
  • Author(s): Kawamoto T, Ohga N, Akiyama K, Hirata N, Kitahara S, Maishi N, Osawa T, Yamamoto K, Kondoh M, Shindoh M, Hida Y, Hida K.
  • Journal Title: PLoS One Volume: 7(3)
  • NAID: 120005946706
  • Peer Reviewed
• [Journal Article] Prostacyclin receptor in tumor endothelial cells promotes angiogenesis in an autocrine manner (2012)
  • Author(s): Osawa T, Ohga N, Hida Y, Kitayama K, Akiyama K, Onodera Y, Fujie M, Shinohara N, Shindoh M, Nonomura K, Hida K
  • Journal Title: Cancer Sci Volume: 103(6) Issue: 6 Pages: 1038-1044
  • DOI: 10.1111/j.1349-7006.2012.02261.x
  • Peer Reviewed
• [Journal Article] Biglycan is a aspecific marker and an autocrine angiogenic factor of tumour endothelial cells (2012)
  • Author(s): Yamamoto K, Ohga N, Hida Y, Maishi N, Kawamoto T, Kitayama K, Akiyama K, Osawa T, Kondoh M, Matsuda K, Onodera Y, Fujie M, Kaga K, Hirano S, Shinohara N, Shindoh M, Hida K.
  • Journal Title: Br J Cancer Volume: 106(6) Issue: 6 Pages: 1214-23
  • DOI: 10.1038/bjc.2012.59
  • Peer Reviewed
• [Journal Article] Differential expressions of matrix metalloproteinases, a disintegrin and metalloproteinases, and a disintegrin and metalloproteinases with thrombospondin motifs and their endogenous inhibitors among histologic subtypes of lung cancers (2012)
  • Author(s): Hida Y, Hamada JI.
  • Journal Title: Anticancer Agents Med Chem
  • Peer Reviewed
• [Journal Article] Tumor endothelial cells acquire drug resistance by MDR1 up-regulation via VEGF signaling in tumor microenvironment (2012)
  • Author(s): Akiyama K, Ohga N, Hida Y, Kawamoto T, Sadamoto Y, Ishikawa S, Maishi N, Akino T, Kondoh M, Matsuda A, Inoue N, Shindoh M, Hida K.
  • Journal Title: Am J Pathol Volume: 180(3) Pages: 1283-93
  • Peer Reviewed
• [Journal Article] Heterogeneity of tumor endothelial cells : comparison between tumor endothelial cells isolated from high-and low-metastatic tumors (2012)
  • Author(s): Ohga N, Ishikawa S, Maishi N, Akiyama K, Hida Y, Kawamoto T, Sadamoto Y, Osawa T, Yamamoto K, Kondoh M, Ohmura H, Shinohara N, Nonomura K, Shindoh M, Hida K.
  • Journal Title: Am J Pathol Volume: 180(3) Pages: 1297-307
  • Peer Reviewed
• [Journal Article] Cyclooxygenase-2 inhibition causes antiangiogenic effects on tumor endothelial and vascular progenitor cells (2012)
  • Author(s): Muraki C, Ohga N, Hida Y, Nishihara H, Kato Y, Tsuchiya K, Matsuda K, Totsuka Y, Shindoh M, Hida K.
  • Journal Title: Int J Cancer Volume: 130(1) Issue: 1 Pages: 59-70
  • DOI: 10.1002/ijc.25976
  • Peer Reviewed
• [Journal Article] Altered angiogenesis in the tumor microenvironment (2011)
  • Author(s): Hida K, Kawamoto T, Ohga N, Akiyama K, Hida Y, Shindoh M.
  • Journal Title: Pathol Int Volume: 61(11) Issue: 11 Pages: 630-7
  • DOI: 10.1111/j.1440-1827.2011.02726.x
  • NAID: 10031185133
  • Peer Reviewed
• [Journal Article] Imatinib mesylate inhibits the proliferation-stimulating effect of human lung cancer-associated stromal fibroblasts on lung cancer cells (2010)
  • Author(s): Kinoshita K, Nakagawa K, Hamada J, Hida Y, Tada M, Kondo S, Moriuchi T.
  • Journal Title: Int J Oncol Volume: 37(4) Pages: 869-77
  • Peer Reviewed
• [Journal Article] Adrenomedullin antagonist suppresses tumor formation in renal cell carcinoma through inhibitory effects on tumor endothelial cells and endothelial progenitor mobilization (2010)
  • Author(s): Tsuchiya K, Hida K, Hida Y, Muraki C, Ohga N, Akino T, Kondo T, Miseki T, Nakagawa K, Shindoh M, Harabayashi T, Shinohara N, Nonomura K, Kobayashi M.
  • Journal Title: Int J Oncol Volume: 36(6) Pages: 1379-86
  • NAID: 120003370695
  • Peer Reviewed
• [Journal Article] Isolation and culture of microvascular endothelial cells from murine inguinal and epididymal adipose tissues (2010)
  • Author(s): Kajimoto K, Hossen MN, Hida K, Ohga N, Akita H, Hyodo M, Hida Y, Harashima H.
  • Journal Title: J Immunol Methods Volume: 357(1-2) Pages: 43-50
  • NAID: 120002248665
  • Peer Reviewed
• [Journal Article] Isolated tumor endothelial cells maintain specific character during long-term culture (2010)
  • Author(s): Matsuda K, Ohga N, Hida Y, Muraki C, Tsuchiya K, Kurosu T, Akino T, Shih SC, Totsuka Y, Klagsbrun M, Shindoh M, Hida K.
  • Journal Title: Biochem Biophys Res Commun Volume: 394(4) Pages: 947-54
  • Peer Reviewed
• [Presentation] Biglycan is a specific marker and an autocrine angiogenic factor of tumor endothelial cells (2012)
  • Author(s): Yamamoto K., Ohga N., Hida Y., Maishi N., Kawamoto T., Kitayama K., Akiyama K., Osawa T., Kondoh M., Kaga K., Hirano S., Shinohara N., Shindoh M., Hida K.
  • Organizer: AACR ANNUAL MEETING 2012
  • Place of Presentation: Chicago, Illinois
  • Year and Date: 2012-04-02
• [Presentation] 腫瘍血管内皮細胞とがん転移との相互作用解析 (2011)
  • Author(s): 間石奈湖、大賀則孝、樋田泰浩、大場雄介、浜田淳一、秋山廣輔、山本和幸、大澤崇宏、近藤美弥子、川本泰輔、進藤正信、井上農夫男、樋田京子
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場(名古屋)
  • Year and Date: 2011-10-05
• [Presentation] 腫瘍血管内皮におけるBiglycanの機能解析 (2011)
  • Author(s): 山本和幸、大賀則孝、樋田泰浩、北山和子、間石奈湖、大澤崇宏、川本泰輔、近藤美弥子、秋山廣輔、加賀基知三、平野聡、進藤正信、樋田京子
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場(名古屋)
  • Year and Date: 2011-10-03
• [Presentation] 腫瘍血管内皮細胞とがん転移との相互作用解析 (2011)
  • Author(s): 間石奈湖、大賀則孝、樋田泰浩、大場雄介、浜田淳一、秋山廣輔、山本和幸、大澤崇宏、近藤美弥子、川本泰輔、井上農夫男、樋田京子
  • Organizer: 第20回日本がん転移学会学術集会・総会
  • Place of Presentation: アクトシティ浜松(浜松)
  • Year and Date: 2011-06-30
• [Presentation] Analysis of interaction between tumor endothelial cells and tumor cells (2011)
  • Author(s): Maishi N., Ohga N., Hida Y., Ohba Y., Kawamoto T., Akiyama K., Kitayama K., Kondoh M., Ohsawa T., Yamamoto K., Inoue N., Shindoh M., Hida K.
  • Organizer: AACR 102^<nd> ANNUAL MEETING 2011
  • Place of Presentation: Orlando, Florida
• [Presentation] Analysis of interaction between tumor endothelial cells and tumor cells (2011)
  • Author(s): Maishi N
  • Organizer: AACR 102nd ANNUAL MEETING 2011
  • Place of Presentation: Orlando, Florida
• [Presentation] Analysis ofinteraction between tumor endothelial cells and tumor cells (2010)
  • Author(s): 間石奈湖、大賀則孝、樋田泰浩、大場雄介、秋山廣輔、北山和子、近藤美弥子、川本泰輔、大澤崇宏、山本和幸、井上農夫男、進藤正信、樋田京子
  • Organizer: 第18回日本血管生物医学会学術集会
  • Place of Presentation: 梅田スカイビル(大阪)
• [Presentation] 腫瘍血管内皮細胞と腫瘍細胞との相互作用解析 (2010)
  • Author(s): 間石奈湖、大賀則孝、黒須拓郎、秋山廣輔、石川修平、近藤美弥子、川本泰輔、樋田泰浩、井上農夫男、樋田京子
  • Organizer: 第19回日本がん転移学会学術集会・総会
  • Place of Presentation: 金沢市文化ホール(金沢)
• [Book] 血管生物医学事典 (2011)
  • Author(s): 樋田京子:佐藤靖史、森田育男、高倉伸幸、小室一成監修
  • Publisher: 朝倉書店
• [Remarks]
  • URL: http://www.den.hokudai.ac.jp/vascular-biology/publications.html