The clinical significance of ING2 expression in colorectal cancr

• Project/Area Number: 21591728
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Fukushima Medical University (2013); Saitama Medical University (2009-2012)
• Principal Investigator: KUMAMOTO Kensuke 福島県立医科大学, 医学部, 講師 (60457778)
• Co-Investigator(Kenkyū-buntansha): TAKENOSHITA Seiichi 福島県立医科大学, 医学部, 教授 (10167489)
• Project Period (FY): 2009-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2013: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000); Fiscal Year 2012: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000); Fiscal Year 2011: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000); Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
• Keywords: 大腸癌 / ING2 / P53 / p53

Research Abstract

The biological significance of the ING2 gene expression in colon cancer will be clarified by in vitro experiments using colon cancer cell lines. The aim of this study is to develop the molecules target treatment in clinical practice. In immunohistochemical analysis of ING2 with 130 colorectal cancer, the strong staining was observed in 78 (60%) cases. Overexpression of ING2 induced MMP13 mRNA expression using a colon cancer cell line, HCT116, into which adenoviral ING2 expression vector was transfected, in cDNA microarray analysis. ING2 overexpressed cells acquired the ability of invasion, leading to suggest that enhanced ING2 expression in colorectal cancer might be associated with cellular invasion and metastasis. Weak expression of ING2 was associated with the effect of preoperative chemoradiotherapy for advanced lower rectal cancer. Therefore ING2 could be the useful marker for the effect of preoperative chemoradiotherapy for advanced lower rectal cancer.

Research Products

• [Journal Article] Polymorphisms of GSTP1, ERCC2 and TS-3'UTR are associated with the clinical outcome of mFOLFOX6 in colorectal cancer patients. (2013)
  • Author(s): Kumamoto K, Ishibashi K, Okada N, Tajima Y, Kuwabara K, Kumagai Y, Baba H, Haga N, Ishida H.
  • Journal Title: Oncology Letters Volume: 6 Pages: 648-654
  • Peer Reviewed
• [Journal Article] 大腸癌-最新の研究動向- VIII.大腸癌の治療戦略化学療法FOLFOX療法の治療効果予測因子の探索(TS、ERCC1など) (2011)
  • Author(s): 隈元謙介、石橋敬一郎、石田秀行
  • Journal Title: 日本臨牀 Volume: 69巻 Pages: 494-499
• [Journal Article] 特集消化器癌における分子標的治療4.大腸癌における分子標的治療a)切除不能大腸癌における分子標的治療 (2011)
  • Author(s): 隈元謙介、石橋敬一郎、石田秀行
  • Journal Title: 外科 Volume: 73巻 Pages: 243-251
• [Journal Article] Targeted disruption of Ing2 results in defective spermatogenesis and development of soft-tissue sarcomas (2010)
  • Author(s): Saito M、Kumamoto K、Robles AI、Horikawa I、Furusato B、Okamura S、Goto A、Yamashita T、Nagashima M、Lee TL、Baxendale VJ、Rennert OM、Takenoshita S、Yokota J、Sesterhenn IA、Trivers GE、Hussain SP、Harris CC
  • Journal Title: PLoS One Volume: 5巻
  • Peer Reviewed
• [Journal Article] Targeted disruption of Ing2 results in defective spermatogenesis and development of soft-tissue sarcomas. (2010)
  • Author(s): Saito M, Kumamoto K, et al.
  • Journal Title: PLoS One Volume: 5 Pages: 15541-15541
  • Peer Reviewed
• [Journal Article] ING2 (2009)
  • Author(s): 隈元謙介
  • Journal Title: G.I. Research Volume: 17巻 Pages: 87-88
• [Journal Article] ING2 is upregulated in colon cancer and increases invasion by enhanced MMP13 expression (2009)
  • Author(s): Kumamoto K、Fujita K、Kurotani R、Saito M、Unoki M、Hagiwara N、Shiga H、Bowman ED、Yanaihara N、Okamura S、Nagashima M、Miyamoto K、Takenoshita S、Yokota J、Harris CC
  • Journal Title: Int J Cancer Volume: 125巻 Pages: 1306-1315
  • Peer Reviewed
• [Journal Article] Reviewing the currentclassification of inhibitor of growth family proteins (2009)
  • Author(s): Unoki M、Kumamoto K、Takenoshita S、Harris CC
  • Journal Title: Cancer Sci Volume: 100巻 Pages: 1173-1179
  • NAID: 10027191680
  • Peer Reviewed
• [Journal Article] ING2 (2009)
  • Author(s): 隈元謙介
  • Journal Title: G.I.Research 17 Pages: 87-88
• [Journal Article] ING2 is upregulated in colon cancer and increases invasion by enhanced MMP13 expression (2009)
  • Author(s): 隈元謙介, et al.
  • Journal Title: Int J Cancer 125 Pages: 1306-1315
  • Peer Reviewed
• [Presentation] 食道癌に対する放射線化学療法の効果予測因子としてのInhibitor of Growth2(ING2) (2011)
  • Author(s): 中村泉, 隈元謙介, ほか
  • Organizer: 日本癌治療学会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2011-10-28
• [Presentation] 当科におけるStageIV大腸癌の治療成績の検討 (2011)
  • Author(s): 隈元謙介、石橋敬一郎、天野邦彦、桑原公亀、大澤智徳、岡田典倫、岩間毅夫、石田秀行
  • Organizer: 第66回日本大腸肛門病学会学術集会
• [Presentation] 大腸癌におけるTS,ERCC1遺伝子発現とFOLFOX治療効果の検討 (2011)
  • Author(s): 隈元謙介, 石橋敬一郎, 岡田典倫, 田島雄介, 幡野哲, 天野邦彦, 桑原公亀, 大澤智徳, 芳賀紀裕, 石田秀行
  • Organizer: 第111回日本外科学会定期学術集会
  • Place of Presentation: (誌上開催)
• [Presentation] PD1-14下部直腸癌に対する術前化学放射線療法の有用性の検討と治療予測因子の探索 (2009)
  • Author(s): 隈元謙介、門馬智之、鈴木聡、中村泉、大木進司、竹之下誠一
  • Organizer: 第64回日本大腸肛門病学会学術集会