The development of new treatment for improvement of microcirculation in cirrhotic liver
| Project/Area Number |
21591755
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
MAEHARA Yoshihiko 九州大学, 医学研究院, 教授 (80165662)
TOMIKAWA Morimasa 九州大学, 大学病院, 准教授 (60325454)
MURATA Masaharu 九州大学, 医学研究院, 准教授 (30304744)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 肝硬変 / 肝微小循環 / Rho kinase, eNOS / ET-1 / Nitric oxide (NO / ナノカプセル / rho-kinase / VEGF / 脾臓 / 門脈圧亢進症 / Rho kinase阻害剤 / Shear response factor |
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| Research Abstract |
Study 1. RhoA and Rho kinase expressions were significantly over expressed in cirrhotic liver accompanied with decrease in eNOS activity. Splenectomy significantly reduce portal vein pressure and improved disturbance of microcirculation in the cirrotic liver. In the liver of cirrhotic rat, Splenectomy also decreased serum ET-1 levels. The ET-1 level was strongly related RhoA activation, and decrease of eNOS phosphorylation. ET-1 derived from the spleen increase intrahepatic resistance by down-regulating Rho signaling in liver cirrhosis.
Study 2. Splenectomy induced thrombocytosis, and increased serotonin content in cirrhotic liver. Stimulation of liver regeneration was indicated by the following parameters : hepatocyte ratio to the entire liver area, Ki67-positive hepatocyte count, and expression of phosphorylated extracellular
signal-regulated kinases. This enhancement of liver regenerationwas negated by ketanserin. Our results showed that splenectomy promoted liver regeneration by increasing serotonin content in liver even under cirrhotic conditions.
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Report
(4 results)
Research Products
(51 results)
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[Journal Article] Liver regeneration is promoted by increasing serotonin content in rat liver with secondary biliary cirrhosis2011
Author(s)
Nagao Y, Akahoshi T, Kamori M, Uehara H, Hashimoto N, Kinjo N, Shirabe K, Taketomi A, Tomikawa M, Hashizume M, Maehara Y
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Journal Title
Hepatol Res
Volume: 41(8)
Pages: 784-94
NAID
Related Report
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[Journal Article] Modulation of CD4^+ T cell responses following splenectomy in hepatitis C virus-related liver cirrhosis2011
Author(s)
12. Hashimoto N, Shimoda S, Kawanaka H, Tsuneyama K, Uehara H, Akahoshi T, Kinjo N, Taketomi A, Shirabe K, Akashi K, Lleo A, Ansari AA, Gershwin ME, Maehara Y
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Journal Title
ClinExpImmunol
Volume: 165(2)
Pages: 243-50
Related Report
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[Journal Article] Impact of antithrombin III concentrates on portal vein thrombosis after splenectomy in patients with liver cirrhosis and hypersplenism2010
Author(s)
6. Kawanaka H, Akahoshi T, Kinjo N, Konishi K, Yoshida D, Anegawa G, Yamaguchi S, Uehara H, Hashimoto N, Tsutsumi N, Tomikawa M, Maehara Y
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Journal Title
Ann Surg
Volume: 251(1)
Pages: 76-83
Related Report
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[Journal Article] Effect of thrombopoietin on platelet counts and liver regeneration after partial hepatectomy in a rat model2009
Author(s)
Shimabukuro R, Kawanaka H, Tomikawa M, Akahoshi T, Konishi K, Yoshida D, Anegawa G, Uehara H, Hashimoto N, Hashizume M, Maehara Y.
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Journal Title
Surg Today
Volume: 39(12)
Pages: 1054-9
NAID
Related Report
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