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The role of Aquaporins in normal gall bladder epithelium and gall bladder cancer

Research Project

Project/Area Number 21591770
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionUniversity of Toyama

Principal Investigator

TSUKADA Kazuhiro  富山大学, 大学院・医学薬学研究部(医学), 教授 (90171967)

Co-Investigator(Kenkyū-buntansha) SHIMADA Yutaka  富山大学, 大学院・医学薬学研究部(医学), 准教授 (30216072)
OKUMURA Tomoyuki  富山大学, 大学院・医学薬学研究部(医学), 助教 (10533523)
YOSHIOKA Isaku  富山大学, 大学病院, 助教 (30436430)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords胆道外科学 / 予後 / 組織アレイ / 発癌 / 胆嚢癌 / AQP-5 / miRNA / RT-PCR
Research Abstract

We examined AQP expression of gall bladder cancer by tissue microarray. In gall bladder cancer, expression of AQP-1 and AQP-5 was observed in 41% and 59% respectively. AQP-5 expression had inverse association with depth of tumor and positive association with gemcitabine sensitivity. Thus, the patients with positive AQP-5 expression had favorable prognosis. SiRNA treatment for AQP-5 positive gall bladder cancer cell line(NOZ-1) inhibited the growth, invasion and mobility of the cells. We identified several miRNAs that can predict Gemcitabine sensitivity of NOZ-1 by microarray analysis. We also identified the target proteins of detected miRNA. Our results suggest that AQP-5 is promising target of gall bladder cancer treatment.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (20 results)

All 2012 2011 2010 2009

All Journal Article (8 results) (of which Peer Reviewed: 4 results) Presentation (10 results) Book (2 results)

  • [Journal Article] Prognostic significance of aquaporins inhuman biliary tract carcinoma2012

    • Author(s)
      Sekine S、Shimada Y, Nagata T, Moriyama M, Omura T, Watanabe T, Hori R, Yoshioka I, Okumura T, Sawada S, Fukuoka J, Tsukada K
    • Journal Title

      Oncology Reports

    • DOI

      10.3892/or.2012.1747

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Prognostic significance of aquaporins inhuman biliary tract carcinoma2012

    • Author(s)
      Sekine S
    • Journal Title

      Oncology Reports

      Volume: (In press)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Involvement of aquaporin-5 in differentiation of human gastric cancerCells2009

    • Author(s)
      Watanabe T, Fujii T, Oya T, Horikawa N, Tabuchi Y, Takahashi Y, Mori M, Takeguchi N, Tsukada K, Sakai H
    • Journal Title

      J Physiol Sci

      Volume: 59 Pages: 113-122

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] 手術の王道II-肝・胆・膵・脾の手術胆嚢癌手術-必要最小限手術の選択2009

    • Author(s)
      塚田一博嶋田裕, 坂東正
    • Journal Title

      消化器外科

      Volume: 31 Pages: 1985-1991

    • Related Report
      2011 Final Research Report
  • [Journal Article] 関根慎一消化器癌の診断・治療胆嚢癌診断と外科治療の選択2009

    • Author(s)
      塚田一博、.嶋田裕, 長田拓哉, 澤田成朗, 森田誠市, 奥村知之, 吉岡伊作, 松井恒志, 大澤宗士
    • Journal Title

      消化器外科

      Volume: 32 Pages: 869-873

    • Related Report
      2011 Final Research Report
  • [Journal Article] Involvement of aquaporin-5 in differentiation of human gastric cancer cells2009

    • Author(s)
      Watanabe T, et al
    • Journal Title

      J Physiol Sci 59

      Pages: 113-122

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 手術の王道II-肝・胆・膵・脾の手術 胆嚢癌手術-必要最小限手術の選択2009

    • Author(s)
      塚田一博, 他
    • Journal Title

      消化器外科 31

      Pages: 1985-1991

    • Related Report
      2009 Annual Research Report
  • [Journal Article] 消化器癌の診断・治療 胆嚢癌 診断と外科治療の選択2009

    • Author(s)
      塚田一博
    • Journal Title

      消化器外科 32

      Pages: 869-873

    • Related Report
      2009 Annual Research Report
  • [Presentation] 胆嚢癌におけるAquaporin発現と腫瘍進展について2011

    • Author(s)
      関根慎一、澤田成朗、森山亮仁、大村哲也、大澤宗士、橋本伊佐也、北條荘三、渡邊智子、吉岡伊作、松井恒志、堀亮太、吉田徹、長田拓哉、魚谷英之、嶋田裕、福岡順也、塚田一博
    • Organizer
      第9回日本消化器外科学会大会
    • Place of Presentation
      福岡
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] The effects of gemcitabine in human gallbladder carcinoma cell lines2011

    • Author(s)
      Sekine S、Sawada S. Moriyama M. Omura T. Yoshioka I. Matsui K. Okumura T. Nagata T. Shimada Y. Tsukada K
    • Organizer
      Asian Pacific HPBA Congress 2011
    • Place of Presentation
      メルボルン
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] 胆嚢疾患におけるAquaporin5の発現について2010

    • Author(s)
      関根慎一、嶋田裕,森山亮仁,小島博文,大澤宗士,北條荘三,渡邊智子,吉岡伊作,松井恒志,堀亮太,奥村知之,澤田成朗,吉田徹,長田拓哉,魚谷英之,塚田一博
    • Organizer
      第8回日本消化器外科学会大会
    • Place of Presentation
      横浜
    • Year and Date
      2010-10-15
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] 組織アレイによる消化器癌における網羅的Aauaporin発現解析2010

    • Author(s)
      嶋田裕、奥村知之,大澤宗士,関根慎一,森山亮仁,小島博文,北條荘三,山口哲也,渡邊智子,松井恒志,吉岡伊作,澤田成朗,吉田徹,長田拓哉,魚谷英之,塚田一博
    • Organizer
      第96回日本消化器病学会
    • Place of Presentation
      新潟
    • Year and Date
      2010-04-22
    • Related Report
      2011 Final Research Report
  • [Presentation] 組織アレイによる消化器癌における網羅的Aauapohn発現解析2010

    • Author(s)
      嶋田裕
    • Organizer
      第96回日本消化器病学会
    • Place of Presentation
      新潟
    • Year and Date
      2010-04-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] Prognostic significance of Aquapolin(AQP) expression in gastric and colorectal cancer2010

    • Author(s)
      Osawa S、Shimada Y., Sekine S., Sawada S., Kashima S., Fukuoka J., Okumura T., Tsukada K
    • Organizer
      American Association of Cancer Research
    • Place of Presentation
      Washington
    • Year and Date
      2010-04-19
    • Related Report
      2011 Final Research Report
  • [Presentation] Prognostic significance of Aquapolin (AQP) expression in gastric and colorectal cancer.2010

    • Author(s)
      Osawa S
    • Organizer
      American Association of Cancer Research
    • Place of Presentation
      Washington
    • Year and Date
      2010-04-19
    • Related Report
      2010 Annual Research Report
  • [Presentation] 胆道癌・膵癌におけるAquaporin 1,4,5,8発現の解析2010

    • Author(s)
      関根慎一
    • Organizer
      第110回日本外科学会定期学術集会
    • Place of Presentation
      名古屋
    • Year and Date
      2010-04-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] ヒト胆嚢癌におけるAquaporinの発現解析2010

    • Author(s)
      関根慎一、嶋田裕,大澤宗士,北條荘三,奥村知之,澤田成朗,長田拓哉,塚田一博
    • Organizer
      第69回日本癌学会学術総会
    • Place of Presentation
      大阪
    • Related Report
      2011 Final Research Report
  • [Presentation] ヒト胃癌におけるAquaporin-5の発現及び臨床経過についての検討2009

    • Author(s)
      渡邊智子、酒井秀紀,塚田一博,嶋田裕,廣川慎一郎,魚谷英之,長田拓哉,田澤賢一,吉田徹,澤田成朗,森田誠市
    • Organizer
      第109回日本外科学会定期学術集会
    • Place of Presentation
      福岡
    • Year and Date
      2009-04-03
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Book] Digestive Surgery胆・膵外科手術2010

    • Author(s)
      塚田一博、奥村知之,吉岡伊作
    • Publisher
      メディカルビュー社
    • Related Report
      2011 Final Research Report
  • [Book] 「Digestive Surgery胆・膵外科手術」胆嚢癌:胆嚢床切除~S4a・S5切除,胆管切除.2010

    • Author(s)
      塚田一博
    • Total Pages
      16
    • Publisher
      メジカルビュー社
    • Related Report
      2010 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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