Development of new neuro-protective and normo-thrermic hibernation therapy using intra-cisternal infusion of magnesium sulfate solution

• Project/Area Number: 21591857
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cerebral neurosurgery
• Research Institution: Juntendo University
• Principal Investigator: MORI Kentaro 順天堂大学, 医学部, 教授 (30200364)
• Co-Investigator(Kenkyū-buntansha): YAMAMOTO Takuji 順天堂大学, 医学部, 先任准教授 (50255684) ; NAKAO Yasuaki 順天堂大学, 医学部, 准教授 (50271276)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2009: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: マグネシウム / 脳保護法 / 遅延性脳神経細胞死 / 海馬 / 脳循環代謝 / 遅発性脳神経細胞死 / 脳保護作用 / 脳血流量

Research Abstract

During mammalian hibernation, magnesium ion(Mg^<2+>) concentration in the brain increases. Based on this fact, we developed the new neuro-protective therapy using cisternal or intra-cerebral magnesium sulfate solution infusion. We demonstrated this therapy suppressed the delayed neuronal death in the hippocampal neurons after the global ischemia. We also showed this neuro-protective effect was not mediated by the suppression of the cerebral blood flow and metabolism.

Research Products

• [Journal Article] Effect of intrathecal magnesium sulfate solution injection via a microcatheter in the cisterna magna on cerebral vasospasm in the canine subarachnoid haemorrhage model (2012)
  • Author(s): Mori K, Yamamoto T, Miyazaki M, Hara Y, Aiko Y, Koike N, Sakamoto S, Nakao Y, Esaki T
  • Journal Title: Br J Neurosurg Volume: 26 Pages: 64-68
  • Peer Reviewed
• [Journal Article] Effect of intrathecal magnesium sulfate solution injection via a microcatheter in the cisterna magna on cerebral vasospasm in the canine subarachnoid haemorrhage model (2012)
  • Author(s): Mori K, Yamamoto T, Miyazaki M, Hara Y, Aiko Y, Koike N, Skamoto S, Nakao Y, Esaki T
  • Journal Title: Br J Neurosurgery Volume: 26 Pages: 64-68
  • Peer Reviewed
• [Journal Article] Optimal cerebrospinal fluid magnesium ion concentration for vasodilatory effect and duration after intracisternal injection of magnesium sulfate solution in the canine subarachnoid hemorrhage model (2011)
  • Author(s): Mori K, Yamamoto T, Miyazaki M, Hara Y, Aiko Y, Koike N, Sakamoto S, Nakao Y, Esaki
  • Journal Title: J Neurosurg Volume: 114 Pages: 1168-1175
  • Peer Reviewed
• [Journal Article] Temporal profile of the effects of intracisternal injection of magnesium sulfate solution on vasodilation of spastic cerebral arteries and CSF magnesium ion concentration in the canine SAH model (2011)
  • Author(s): Mori K, Miyazaki M, Hara Y, Aiko Y, Yamamoto T, Nakao Y, Esaki T
  • Journal Title: Acta Neurochir Suppl Volume: 110 Pages: 39-42
  • Peer Reviewed
• [Journal Article] Optimal cerebrospinal fluid magnesium ion concentration for vasodilatory effect and duration after intracisternal injection of magnesium sulfate solution in the canine subarachnoid hemorrhage model (2011)
  • Author(s): Mori K, Yamamoto T, Miyazaki M, Hara Y, Aiko Y, Koike N, Sakamoto S, Nakako Y, Esaki T
  • Journal Title: J Neurosurgery Volume: 114 Pages: 1168-1175
  • Peer Reviewed
• [Journal Article] Optimal cerebrospinal fluid magnesium ion concentration for vasodilatory effect and duration after Intracisternal injection of magnesium sulfa-te solution in a canine subarachnoid hemorrhage model. (2011)
  • Author(s): Mori K, Yamamoto T, Nakao Y, et al
  • Journal Title: J of Neurosurgery Volume: VOL.112(In press)
  • Peer Reviewed
• [Journal Article] Initial clinical experience of vasodilatory effect of intra-cisternal infusion of magnesium sulfate for the treatment of cerebral vasospasm after aneurismal subarachnoid hemorrhage (2009)
  • Author(s): Mori K, Yamamoto T, Nakao Y, Osada H, Hara Y, Oyama K, Esaki T
  • Journal Title: Neurol Med Chir Volume: 49 Pages: 139-144
  • Peer Reviewed
• [Journal Article] Novel vasodilatory effect of intra-cisternal injection of magnesium sulfate solution on spastic cerebral arteries in the canine two-hemorrhage model of subarachnoid hemorrhage (2009)
  • Author(s): Mori K, Miyazaki M, Hara Y, Aiko Y, Yamamoto T, Nakao Y
  • Journal Title: J Neurosurg Volume: 110 Pages: 73-78
  • Peer Reviewed
• [Journal Article] Novel vasodilatory effect of intra-cisternal injection of magnesium sulfate solution on spastic cerebral arteries in the canine two-hemorrhage model of subarachnoid hemorrhage. (2009)
  • Author(s): Mori K, Miyazaki M, Hara Y, Aiko Y, Yamamoto T, Nakao Y
  • Journal Title: J Neurosurgery VOL.110 Pages: 73-78
  • Peer Reviewed
• [Journal Article] Initial clinical experience of vasodilatory effect of intra-cisternal infusion of magnesium sulfate for the treatment of cerebral vasospasm after aneurismal subarachnoid hemorrhage. (2009)
  • Author(s): Mori K, Yamamoto T, Nakao Y, Osada H, Hara Y, Oyama K, Esaki T
  • Journal Title: Neurol Med Chir(Tokyo) VOL.49 Pages: 139-144
  • Peer Reviewed
• [Presentation] 硫酸マグネシウム溶液の脳保護作用がSAH後のearly brain injuryによる脳虚血損傷を軽減する可能性について (2012)
  • Author(s): 森健太郎、山本拓史、中尾保秋、江崎孝徳
  • Organizer: 第28回スパズムシンポジウム
  • Place of Presentation: 福岡
  • Year and Date: 2012-04-26
• [Presentation] 脳脊髄液中のマグネシウムイオン濃度がくも膜下出血後の脳血管レン縮に及ぼす影響 (2012)
  • Author(s): 森健太郎
  • Organizer: 第89回日本整理学会大会
  • Place of Presentation: 松本
  • Year and Date: 2012-03-29
• [Presentation] 脳槽内硫酸マグネシウム投与によるレン縮血管拡張作用の至適脳脊髄液Mg ion濃度と作用時間の定量的検討 (2011)
  • Author(s): 森健太郎、山本拓史、中尾保秋、江崎孝徳
  • Organizer: 第27回スパズムシンポジウム
  • Place of Presentation: 横浜
  • Year and Date: 2011-09-10
• [Presentation] 脳槽内硫酸マグネシウム溶液投与によるレン縮血管拡張作用の至適脳脊髄液Mg ion濃度と作用時間の定量的検討 (2011)
  • Author(s): 森健太郎、山本拓史、中尾保秋、江崎孝徳
  • Organizer: 第27回スパズム
  • Place of Presentation: 京都
  • Year and Date: 2011-07-29
• [Presentation] Intracisternal magnesium injection therapy(Experimental data for its clinical application) (2011)
  • Author(s): Mori K, Yamamoto T, Hasegawa H, Nakao Y, Esaki T
  • Organizer: 11th International Conference of Neurovascular Events after SAH(Vasospasm 2011)
  • Place of Presentation: Cincinnati
  • Year and Date: 2011-07-21
• [Presentation] Intracisternal magnesium injection therapy (Experimental data for its clinical application) (2011)
  • Author(s): Mori K, Yamamoto T, Hasegawa H, Nakao Y, Esaki T
  • Organizer: 11^<th> International Conference of Neurovascular Events after SAH (Vasospasm 2011)
  • Place of Presentation: Cincinnati
  • Year and Date: 2011-07-21
• [Presentation] Intracisternal infusion of magnesium sulfate after aneurysmal subarachnoid hemorrhage : What is the evidence? (2011)
  • Author(s): Mori K
  • Organizer: International STROKE Conference 2011
  • Place of Presentation: Los Angeles
  • Year and Date: 2011-02-10
• [Presentation] Intracisternal infusion of magnesium sulphate after aneurismal subarac-hnoid hemorrhage ; what is the evidence? (2011)
  • Author(s): Mori K
  • Organizer: International stroke conference
  • Place of Presentation: Los Angeles
  • Year and Date: 2011-02-10
• [Presentation] 硫酸マグネシウム(MgSO4)溶液脳槽内投与によるコイル塞栓術後の脳血管攣縮治療法の開発 (2010)
  • Author(s): 森健太郎、山本拓史、中尾保秋、江崎孝徳
  • Organizer: 第26回スパズム・シンポジウム
  • Place of Presentation: 盛岡
  • Year and Date: 2010-04-15
• [Presentation] Temporal profile of the effects of intracisternal injection of magnesium sulfate solution on vasodilation of spastic cerebral arteries and CSF magnesium ion concentration in the canine SAH model (2009)
  • Author(s): Mori K
  • Organizer: 10^<th> international Conference on Cerebral Vasospasm
  • Place of Presentation: Chongqing, China
  • Year and Date: 2009-10-09
• [Presentation] 硫酸マグネシウム溶液の脳槽内持続注入による症候性脳血管レン縮の治療 (2009)
  • Author(s): 森健太郎、山本拓史、中尾保秋
  • Organizer: 第25回スパズム・シンポジウム
  • Place of Presentation: 島根
  • Year and Date: 2009-03-20
• [Presentation] 犬およびRatクモ膜下出血モデルを用いた脳槽内マグネシウム(Mg)投与による脳血管レン縮改善効果 (2008)
  • Author(s): 森健太郎、宮崎正弘、原保和、愛甲泰久
  • Organizer: 第24回スパズムシンポジウム
  • Place of Presentation: 京都
  • Year and Date: 2008-03-20
• [Presentation] Intra-cisternal infusion of magnesium solution reverses the reduced cerebral blood flow induced by experimental subarachnoid hemorrhage in the rat (2007)
  • Author(s): Kentaro Mori, Masahiro Miyazaki, Junko Iwata
  • Organizer: Brain' 07 & BrainPET'
  • Place of Presentation: 大阪
  • Year and Date: 2007-05-23