Magnesium enhances adherence and cartilage formation of synovial mesenchymal stem cells through integrins
| Project/Area Number |
21591914
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
SEKIYA Ichiro 東京医科歯科大学, 大学院・医歯学総合研究科, 寄附講座教員 (10345291)
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| Co-Investigator(Renkei-kenkyūsha) |
MUNETA Takeshi 東京医科歯科大学, 医歯学総合研究科, 教授 (50190864)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 間葉幹細胞 / 滑膜 / 軟骨 / マグネシウム / インテグリン |
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| Research Abstract |
We previously reported that more than 60% of synovial mesenchymal stem cells(MSCs) placed on osteochondral defects adhered to the defect within 10 minutes and promoted cartilage regeneration. In this study, we investigated whether magnesium enhanced adherence and chondrogenesis of synovial MSC through integrins. Magnesium increased adhesion of human synovial MSCs to collagen and promoted synthesis of cartilage matrix during in vitro chondrogenesis of synovial MSCs. In vivo studies in rabbits showed that magnesium promoted adherence at 1 day and cartilage formation of synovial MCSs at 2 and 4 weeks. Magnesium enhanced adherence of synovial MSCs and promoted synthesis of cartilage matrix at an early phase.
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Report
(4 results)
Research Products
(39 results)
