Magnesium enhances adherence and cartilage formation of synovial mesenchymal stem cells through integrins

• Project/Area Number: 21591914
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: SEKIYA Ichiro 東京医科歯科大学, 大学院・医歯学総合研究科, 寄附講座教員 (10345291)
• Co-Investigator(Renkei-kenkyūsha): MUNETA Takeshi 東京医科歯科大学, 医歯学総合研究科, 教授 (50190864)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 間葉幹細胞 / 滑膜 / 軟骨 / マグネシウム / インテグリン

Research Abstract

We previously reported that more than 60% of synovial mesenchymal stem cells(MSCs) placed on osteochondral defects adhered to the defect within 10 minutes and promoted cartilage regeneration. In this study, we investigated whether magnesium enhanced adherence and chondrogenesis of synovial MSC through integrins. Magnesium increased adhesion of human synovial MSCs to collagen and promoted synthesis of cartilage matrix during in vitro chondrogenesis of synovial MSCs. In vivo studies in rabbits showed that magnesium promoted adherence at 1 day and cartilage formation of synovial MCSs at 2 and 4 weeks. Magnesium enhanced adherence of synovial MSCs and promoted synthesis of cartilage matrix at an early phase.

Research Products

• [Journal Article] 滑膜間葉幹細胞の役割と低侵襲な軟骨再生への応用 (2012)
  • Author(s): 関矢一郎、宗田大
  • Journal Title: 雑誌整形外科 Volume: Vol.63, No.3 Pages: 228-228
• [Journal Article] 変形性膝関節症をめぐる進歩 (2012)
  • Author(s): 関矢一郎、宗田大
  • Journal Title: 滑膜由来の幹細胞による再生医療 Volume: Vol.2, No.1 Pages: 159-165
• [Journal Article] 滑膜由来の幹細胞による再生医療 (2012)
  • Author(s): 関矢一郎、宗田大
  • Journal Title: Bone Joint Nerve Volume: Vol.2, No.1 Pages: 159-165
• [Journal Article] 滑膜幹細胞を用いた関節軟骨再生 (2011)
  • Author(s): 関矢一郎、宗田大
  • Journal Title: 治療 Volume: Vol.93, No.8 Pages: 1784-1793
• [Journal Article] 滑膜間葉幹細胞を用いた関節軟骨再生 (2011)
  • Author(s): 関矢一郎
  • Journal Title: クリニカルカルシウム Volume: Vol.21 No.6 Pages: 83-93
• [Journal Article] 軟骨再生の概要と応用の可能性 (2011)
  • Author(s): 関矢一郎
  • Journal Title: 医事新報 Volume: No.4548 Pages: 59-61
• [Journal Article] 再生医学のいま基礎研究から臨床への展開に向けて (2011)
  • Author(s): 関矢一郎、宗田大
  • Journal Title: 治療 Volume: Vol.93, No.8 Pages: 1784-1793
• [Journal Article] 軟骨再生の概要と応用の可能性 (2011)
  • Author(s): 関矢一郎
  • Journal Title: 日本医事新報 Volume: No.4548 Pages: 59-61
• [Journal Article] Magnesium enhances adherence andcartilage formation of synovialmesenchymal stem cells through integrins (2010)
  • Author(s): Shimaya M, Muneta T, Ichinose S, Tsuji K, Sekiya I
  • Journal Title: Osteoarthritis Cartilage Volume: Oct ; 18(10) Pages: 1300-9
  • Peer Reviewed
• [Journal Article] Magnesium enhances adherence and cartilage formation ofsynovial mesenchymal stem cells through integrins. (2010)
  • Author(s): Shimaya M, Muneta T, Ichinose S, Tsuji K, Sekiya I.
  • Journal Title: Osteoarthritis and Cartilage Volume: 10 Pages: 1300-1309
  • Peer Reviewed
• [Presentation] Cartilage regeneration with synovial stemcells (2012)
  • Author(s): 関矢一郎
  • Organizer: The 9th International Symposium for Orthopaedic Sports Medicine
  • Place of Presentation: Taiwan
  • Year and Date: 2012-03-24
• [Presentation] Cartilage regeneration with synovial stem cells (2012)
  • Author(s): 関矢一郎
  • Organizer: The 9th International Symposium for Orthopaedic Sports Medicine
  • Place of Presentation: Keelung, Taiwan
  • Year and Date: 2012-03-24
• [Presentation] 滑膜幹細胞による軟骨再生 (2012)
  • Author(s): 関矢一郎
  • Organizer: 再生医療カンファレンス
  • Place of Presentation: 東京大学
  • Year and Date: 2012-02-16
• [Presentation] 滑膜幹細胞による軟骨再生 (2012)
  • Author(s): 関矢一郎
  • Organizer: 東京大学再生医療カンファレンス
  • Place of Presentation: 東京大学
  • Year and Date: 2012-02-16
• [Presentation] 幹細胞による軟骨再生-現状と展望- (2011)
  • Author(s): 関矢一郎
  • Organizer: 日本臨床スポーツ医学会シンポジウム
  • Place of Presentation: 青森
  • Year and Date: 2011-11-06
• [Presentation] 滑膜幹細胞の役割と低侵襲な軟骨再生への応用 (2011)
  • Author(s): 関矢一郎
  • Organizer: 日本整形外科学会第26回基礎学術集会
  • Place of Presentation: ランチョンセミナー
  • Year and Date: 2011-10-20
• [Presentation] 滑膜幹細胞の役割と低侵襲な軟骨再生への応用 (2011)
  • Author(s): 関矢一郎
  • Organizer: 日本整形外科学会第26回基礎学術集会ランチョンセミナー
  • Place of Presentation: 前橋
  • Year and Date: 2011-10-20
• [Presentation] Roles of stem cells in synovial fluid andclinical applications of stem cells fromsynovium (2011)
  • Author(s): 関矢一郎
  • Organizer: Singapore Orthopaedic Association
  • Year and Date: 2011-10-13
• [Presentation] Roles of stem cells in synovial fluid and clinical applications of stem cells from synovium (2011)
  • Author(s): 関矢一郎
  • Organizer: Singapore Orthopaedic Association
  • Place of Presentation: Singapore
  • Year and Date: 2011-10-13
• [Presentation] 滑膜幹細胞による軟骨・半月板再生 (2011)
  • Author(s): 関矢一郎
  • Organizer: 信州運動器診療フォーラム
  • Place of Presentation: 松本
  • Year and Date: 2011-10-01
• [Presentation] Mesenchymal stem cells derived form synovium : their properties and clinical application for cartilage regeneration (2011)
  • Author(s): 関矢一郎
  • Place of Presentation: Seoul
  • Year and Date: 2011-09-30
• [Presentation] 滑膜幹細胞の視点から膝関節疾患の病態と構造体の再生を考える (2011)
  • Author(s): 関矢一郎
  • Organizer: 第10回鹿児島サイトカイン制御療法研究会
  • Year and Date: 2011-09-21
• [Presentation] Mesenchymal stem cells derived form synovium : their properties and clinicalapplication for cartilage regeneration (2011)
  • Author(s): 関矢一郎
  • Organizer: International Summer Program 2011 Tokyo Medical and Dental
  • Place of Presentation: University
  • Year and Date: 2011-08-30
• [Presentation] Mesenchymal stem cells derived form synovium : their properties and clinical application for cartilage regeneration (2011)
  • Author(s): 関矢一郎
  • Organizer: International Summer Program
  • Place of Presentation: Tokyo Medical and Dental University
  • Year and Date: 2011-08-30
• [Presentation] 関節液中の幹細胞と関節内組織損傷 (2011)
  • Author(s): 関矢一郎
  • Organizer: 日本骨代謝学会シンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 2011-07-30
• [Presentation] 滑膜間葉幹細胞による軟骨再生 (2011)
  • Author(s): 関矢一郎
  • Organizer: 日本骨代謝学会シンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 2011-07-28
• [Presentation] Mesenchymal stem cells derived form synovium : their properties and clinicalapplication for cartilage regeneration (2011)
  • Author(s): 関矢一郎
  • Organizer: The 3rd International Cartilage and Osteoarthritis Symposium
  • Place of Presentation: Korea
  • Year and Date: 2011-07-04
• [Presentation] Mesenchymal stem cells derived form synovium : their properties and clinical application for cartilage regeneration (2011)
  • Author(s): 関矢一郎
  • Organizer: The 3rd International Cartilage and Osteoarthritis Symposium
  • Place of Presentation: Suwon, Korea
  • Year and Date: 2011-07-04
• [Presentation] 滑膜幹細胞を関節鏡視下で移植する軟骨再生医療 (2011)
  • Author(s): 関矢一郎
  • Organizer: 東京整形外科画像診断研究会
  • Place of Presentation: 神田
  • Year and Date: 2011-06-11
• [Presentation] 滑膜幹細胞を関節鏡視下で移植する軟骨再生医療 (2011)
  • Author(s): 関矢一郎
  • Organizer: 東京整形外科画像診断研究会
  • Place of Presentation: 東京神田
  • Year and Date: 2011-06-11
• [Presentation] 滑膜間葉幹細胞を用いる低侵襲軟骨再生医療におけるマグネシウムの細胞接着促進効果 (2011)
  • Author(s): 関矢一郎
  • Organizer: 日本軟骨代謝学会シンポジウム
  • Place of Presentation: 博多
  • Year and Date: 2011-03-05
• [Presentation] 滑膜間葉幹細胞を用いる低侵襲軟骨再生医療におけるマグネシウムの細胞接着促進効果 (2011)
  • Author(s): 関矢一郎
  • Organizer: 日本軟骨代謝学会
  • Place of Presentation: 福岡
  • Year and Date: 2011-03-05
• [Presentation] 滑膜幹細胞による軟骨再生医療 (2011)
  • Author(s): 関矢一郎
  • Organizer: 日本再生医療学会シンポジウム
  • Place of Presentation: 新宿
  • Year and Date: 2011-03-02
• [Presentation] Cartilage regeneration with synovial stemcells (2011)
  • Author(s): 関矢一郎
  • Organizer: The International Symposium of Global Center of Excellence ProgramClinical Researches and Experiences for Cartilage Regeneration Therapy
  • Place of Presentation: Tokyo Medical and Dental University
  • Year and Date: 2011-02-03
• [Presentation] Mesenchymal stem cells derived form synovium : their properties and clinicalapplication for cartilage regeneration (2011)
  • Author(s): 関矢一郎
  • Organizer: Morehouse college
  • Place of Presentation: Atlanta
  • Year and Date: 2011-01-08
• [Presentation] Magnesium enhances adherence and cartilage formation of synovial mesenchymal stem cells through integrins (2010)
  • Author(s): 島谷雅之
  • Organizer: Orthopaedic Research Society
  • Place of Presentation: New Orleans, USA
  • Year and Date: 2010-03-06
• [Presentation] マグネシウムはインテグリンを介して滑膜間葉幹細胞の軟骨欠損部への接着と軟骨基質産生を促進する (2009)
  • Author(s): 島谷雅之
  • Organizer: 日本整形外科学会基礎学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2009-11-05
• [Book] 再生医療叢書第6巻骨格系 (2012)
  • Author(s): 関矢一郎、宗田大
  • Publisher: 朝倉書店
• [Remarks]
  • URL: http://www.terumozaidan.or.jp/labo/class/index.html