| Project/Area Number |
21591952
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Oita University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIOKA Hidekatsu 大分大学, 医学部, 教授 (00222430)
HAMANAKA Ryouzi 大分大学, 医学部, 助教 (60274750)
SUMIYOSHI Hideaki 東海大学, 医学部, 研究員 (60343357)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | コラーゲン / 転写因子 / 発現調節 / 骨 / 軟骨 / 転写 / プロモーター |
|---|
| Research Abstract |
The current study characterized the transcriptional regulation of the mouse type XXVII collagen gene(Col27a1). The transcriptional activity was seen from-568 to-445 in the downstream promoter(for Exon1), and EMSA indicated that nuclear protein bound to a region, from-504 to-465. However, no element, such as a chondrocyte-specific enhancer was detected within that region of Col27a1 gene. Finally, an in situ hybridization analysis was performed to demonstrate the expression pattern of the two alternative splicing transcripts. The transcript with Exon1' was expressed in cartilage similar to that of type II collagen, whereas the other with Exon1 was mainly expressed in skin.
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