| Project/Area Number |
21591987
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | Meikai University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SAKAGAMI Hiroshi 明海大学, 歯学部, 教授 (50138484)
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| Project Period (FY) |
2009 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 麻酔 / 蘇生学 / オートファジー / アポトーシス / ネクローシス / 癌細胞 / dexmedetomidine / clonidine / adrenaline / がん細胞 / シダゾラム / ジブカイン / リドカイン / アドレナリン |
|---|
| Research Abstract |
Anesthetics have been administered to malignant tumors and surrounding tissues during the surgery, their effects on oral tissues is not well understood. In the present studies, the cytotoxicity of 1) local anesthetics, such as dibucaine 2) intravenous anesthetics, such as midazolam and 3) adrenergic agonists, such as dexmedetomidine against oral tumor and normal cells was compared among each group. Tumor-specificity index was determined by the ratio of mean 50% cytotoxic concentration against normal cells to that for tumor cells. Apoptosis induction was monitored by internucleosomal DNA fragmentation and caspase-3,-8, and-9 activation. Fine cell structure was observed under transmission electron microscopy. Dibucaine, midazolam and dexmedetomodine did not induce apoptosis in oral squamous cell carcinoma(OSCC) cells although they induced apoptosis in HL-60(human promyelocytic leukemia). Autophagy inhibitors did not reduce the cytotoxicity induced by these drugs. The necrosis may be involved in the induction of antitumor activity by local anesthetics, intravenous anesthetics and adrenergic agonists in OSCC cells. However, further study is needed for another tumor cell lines
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