Autophagy-inducing activity of anesthetics against various malignant cells
| Project/Area Number |
21591987
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | Meikai University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SAKAGAMI Hiroshi 明海大学, 歯学部, 教授 (50138484)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 麻酔 / 蘇生学 / オートファジー / アポトーシス / ネクローシス / 癌細胞 / dexmedetomidine / clonidine / adrenaline / がん細胞 / シダゾラム / ジブカイン / リドカイン / アドレナリン |
|---|
| Research Abstract |
Anesthetics have been administered to malignant tumors and surrounding tissues during the surgery, their effects on oral tissues is not well understood. In the present studies, the cytotoxicity of 1) local anesthetics, such as dibucaine 2) intravenous anesthetics, such as midazolam and 3) adrenergic agonists, such as dexmedetomidine against oral tumor and normal cells was compared among each group. Tumor-specificity index was determined by the ratio of mean 50% cytotoxic concentration against normal cells to that for tumor cells. Apoptosis induction was monitored by internucleosomal DNA fragmentation and caspase-3,-8, and-9 activation. Fine cell structure was observed under transmission electron microscopy. Dibucaine, midazolam and dexmedetomodine did not induce apoptosis in oral squamous cell carcinoma(OSCC) cells although they induced apoptosis in HL-60(human promyelocytic leukemia). Autophagy inhibitors did not reduce the cytotoxicity induced by these drugs. The necrosis may be involved in the induction of antitumor activity by local anesthetics, intravenous anesthetics and adrenergic agonists in OSCC cells. However, further study is needed for another tumor cell lines
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Report
(4 results)
Research Products
(75 results)
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[Journal Article] 亜酸化窒素セボフルラン全身麻酔下ペンタゾシン静脈内投与がBIS値に及ぼす影響について2012
Author(s)
小貫和之, 小貫典子, 長尾泰好, 小宅宏史, 吉川秀明, 森一将, 龍田恒康, 竹島浩, 嶋田淳, 長谷川彰彦, 長坂浩
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Journal Title
明海歯学
Volume: 41巻
Pages: 44-8
Related Report
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[Journal Article] 亜酸化窒素・セボフルラン全身麻酔下でのペンタゾシンがauditory evoked potentialとbispectral indexに及ぼす影響2011
Author(s)
小貫和之, 小宅宏史, 山西優一郎, 内田茂則, 今村敏克, 塚本真規, 吉川秀明, 田島徹, 嶋田淳, 長谷川彰彦, 長坂浩
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Journal Title
日本歯科麻酔学会雑誌
Volume: 39巻
Pages: 628-628
NAID
Related Report
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[Journal Article] BLS(Basic life support)講習会における関心度,モチベーション,技術講習状況についての調査-歯科医療従事受講者と歯科以外の医療従事受講者の比較検討-2011
Author(s)
竹内菊子, 大木良蔵, 大野聖加, 今村敏克, 小貫和之, 中島丘, 高平修二, 長谷川彰彦, 町野守, 嶋田淳, 長坂浩
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Journal Title
明海歯学
Volume: 40巻
Pages: 55-61
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[Book] 歯科麻酔学7版2011
Author(s)
長坂浩, 金子譲, 福島和昭, 原田純, 嶋田昌彦, 一戸達也, 丹羽均
Publisher
医歯薬出版
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