Functional analysis of prostate cancer spheroid forming related genes and its clinical application

• Project/Area Number: 21592034
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Urology
• Research Institution: Yokohama National University
• Principal Investigator: WATANABE Masatoshi 横浜国立大学, 工学研究院, 教授 (90273383)
• Co-Investigator(Kenkyū-buntansha): SHIRAISHI Taizo 三重大学, 医学系研究科, 教授 (30162762)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 前立腺癌 / スフェロイド / siRNA / 前立腺がん

Research Abstract

Some genes including the Plk2were extracted as a candidate gene related with spheroid formation. We analyzed effects of the Plk2on the spheroid characterization and anticancer drug resistance in DU-145 spheroids by suppressing plk2with RNAi. The results indicate that the Plk2may be involved in suppression of tumor growth and acquisition of anticancer drug resistance.

Research Products

• [Journal Article] Application of the Magnetic Nanoparticles to Gene Delivery (2011)
  • Author(s): D. Kami, S. Takeda, Y. Itakura, S. Gojo, M. Watanabe, and M. Toyoda
  • Journal Title: Int. J. Mol. Sci Volume: 12 Issue: 6 Pages: 3705-3722
  • DOI: 10.3390/ijms12063705
  • Peer Reviewed
• [Journal Article] Efficient transfection method using deacylated polyethylenimine-coated magnetic nanoparticles (2011)
  • Author(s): D. Kami, S. Takeda, M. Hatsune, M. Toyoda, Y. Itakura, S. Gojo, S. Kyo, A. Umezawa, M. Watanabe
  • Journal Title: J. Artif. Organs Volume: 14 Issue: 3 Pages: 215-222
  • DOI: 10.1007/s10047-011-0568-6
  • NAID: 10030338996
  • Peer Reviewed
• [Journal Article] 各種ナノ粒子の細胞への影響:細胞特異性とその応用 (2011)
  • Author(s): 一町直樹, 栗岡大輔, 河井一明, 葛西宏, 松本幹治, 渡邉昌俊
  • Journal Title: 粉体工学会誌 Volume: 48 Pages: 145-151
  • NAID: 10027915910
  • Peer Reviewed
• [Journal Article] 前立腺癌化学療法における磁性体ナノ粒子の併用効果の基礎研究 (2011)
  • Author(s): 一町直樹, 佐藤明子, 栗岡大輔, 米田操, 広川佳史, 白石泰三, 渡邉昌俊
  • Volume: 24
  • Pages: 1267-1269
• [Journal Article] Multicellular spheroid culture models : Applications in prostate cancer research and therapeutic (2011)
  • Author(s): D. Kurioka, A. Takagi, M. Yoneda, Y. Hirokawa, T. Shiraishi, M. Watanabe
  • Journal Title: Journal of Cancer Science and Therapy Volume: 3(3) Issue: 03 Pages: 60-65
  • DOI: 10.4172/1948-5956.1000059
  • Peer Reviewed
• [Journal Article] 前立腺癌化学療法における磁性体ナノ粒子の併用効果の基礎研究 (2011)
  • Author(s): 一町直樹, 佐藤明子, 栗岡大輔, 米田操, 広川佳史, 白石泰三, 渡邉昌俊
  • Journal Title: 泌尿器外科 Volume: 24 Pages: 1267-1269
• [Journal Article] Multicellular spheroid culture models : Applications in prostate cancerresearch and therapeutic. (2011)
  • Author(s): D.Kurioka, A.Takagi, M.Yoneda, Y.Hirokawa, T.Shiraishi, M.Watanabe.
  • Journal Title: Journal of Cancer Science and Therapy Volume: 3 Pages: 60-65
  • Peer Reviewed
• [Journal Article] Editorial Comment on Article(Maspin expression in renal cell carcinoma and its correlation with clinicopathologic parameters) (2010)
  • Author(s): M. Watanabe
  • Journal Title: Urology Volume: 76 Issue: 3 Pages: 3-3
  • DOI: 10.1016/j.urology.2010.01.074
  • Peer Reviewed
• [Journal Article] Pathological findings at radical prostectomy in Japanese prospective active surveillance cohort (2010)
  • Author(s): M. Sugimoto, T. Shiraishi, H. Tsunemori, T. Demura, Y. Saito, T. Kamoto, Y. Kakehi
  • Journal Title: Jpn J. Clin Oncol Volume: 40 Issue: 10 Pages: 973-979
  • DOI: 10.1093/jjco/hyq082
  • Peer Reviewed
• [Journal Article] Clinicopathological Research Group for Localized Prostate Cancer Investigators. Discrepancy between local and central pathological review of radical prostatectomy specimens. (2010)
  • Author(s): K. Kuroiwa, T. Shiraishi, O. Ogawa, M. Usami, Y. Hirano, S. Naito
  • Journal Title: J Urol Volume: 183 Pages: 952-957
  • Peer Reviewed
• [Journal Article] 長期経過中に多彩な合併症を呈したMcCune-Albright syndromeの1例 (2010)
  • Author(s): 荒木里香, 三崎盛治, 町野由佳, 中山茂穂, 渡辺昌俊, 渡辺典子, 成瀬光栄
  • Journal Title: ホルモンと臨床 Volume: 58 Pages: 192-197
• [Journal Article] Editorial Comment on Article (Maspin expression in renal cell carcinoma and its correlation with clinicopathologic parameters).(Invited) (2010)
  • Author(s): M.Watanabe.
  • Journal Title: Urology Volume: 76
  • Peer Reviewed
• [Journal Article] RhoB enhances migration and MMP1 expression of prostate cancer DU145 (2009)
  • Author(s): M. Yoneda, Y. S. Hirokawa, A. Ohashi, K. Uchida, D. Kami, M. Watanabe, T. Yokoi, T. Shiraishi, S. Wakusawa
  • Journal Title: Exp. Mol. Pathol Volume: 88 Issue: 1 Pages: 90-95
  • DOI: 10.1016/j.yexmp.2009.09.010
  • Peer Reviewed
• [Journal Article] The chromosomal constitution of postmitotic neurons, assessed by neuronal nuclear transfer into oocytes and in ES cell lines derived from them (2009)
  • Author(s): T. Osada, N. Kakazu, M. Watanabe, H. Yamane, T. Yagi
  • Journal Title: Cytogenet Genome Res Volume: 125 Issue: 3 Pages: 201-212
  • DOI: 10.1159/000230004
  • Peer Reviewed
• [Journal Article] ナノ粒子メディシン (2009)
  • Author(s): 渡邉昌俊
  • Journal Title: 医学のあゆみ Volume: 230 Pages: 493-493
• [Journal Article] RhoB enhances migration and MMP1 expression of prostate cancer DU145 (2009)
  • Author(s): M.Yoneda, Y.S.Hirokawa, A.Ohashi, K.Uchida, D.Kami, M.Watanabe, T.Yokoi, T.Shiraishi, S.Wakusawa
  • Journal Title: Exp.Mol.Pathol. 88 Pages: 90-95
  • Peer Reviewed
• [Journal Article] The chromosomal constitution of postmitotic neurons, assessed by neuronal nuclear transfer into oocytes and in ES cell lines derived from them (2009)
  • Author(s): T.Osada, N.Kakazu, M.Watanabe, H.Yamane, T.Yagi
  • Journal Title: Cytogenet Genome Res. 125 Pages: 201-212
  • Peer Reviewed
• [Journal Article] ナノ粒子メディシン はじめに (2009)
  • Author(s): 渡邉昌俊
  • Journal Title: 医学のあゆみ 230 Pages: 493-493
• [Presentation] 前立腺がんスフェロイドの抗癌剤抵抗性へのplk2遺伝子の関与について (2011)
  • Author(s): 菅原健太郎、讃良茂浩、米田操、広川佳史、白石泰三、高木陽光、渡邉昌俊
  • Organizer: 第70回日本癌学会
  • Place of Presentation: 名古屋国際会議場,名古屋市
  • Year and Date: 2011-10-05
• [Presentation] 前立腺がんスフェロイドの抗癌剤抵抗性へのplk2遺伝子の関与について (2011)
  • Author(s): 菅原健太郎、讃良茂浩、米田操、広川佳史、白石泰三、高木陽光、渡邉昌俊
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場、名古屋
  • Year and Date: 2011-10-05
• [Presentation] 腫瘍と腫瘍微小環境 (2011)
  • Author(s): 渡邉昌俊
  • Organizer: 化学工学会第43回秋季大会シンポジウム
  • Place of Presentation: 名古屋工業大学,名古屋
  • Year and Date: 2011-09-15
• [Presentation] 腫瘍と腫瘍微小環境 (2011)
  • Author(s): 渡邉昌俊
  • Organizer: 化学工学会第43回秋季大会シンポジウム
  • Place of Presentation: 名古屋工業大学、名古屋
  • Year and Date: 2011-09-15
• [Presentation] A prostate cancerspheroid related gene and chemotherapy (2011)
  • Author(s): M. Watanabe, A. Takagi, Y. Hirokawa, T. Shiraishi
  • Organizer: 102nd Annual Meeting of AACR
  • Place of Presentation: Orland, Florida
  • Year and Date: 2011-04-05
• [Presentation] A prostate cancer spheroid related gene and chemotherapy (2011)
  • Author(s): M.Watanabe, A.Takagi, Y.Hirokawa, T.Shiraishi
  • Organizer: 102^<nd> Annual Meeting of American Association for Cancer Research
  • Place of Presentation: Orland, Florida, USA
  • Year and Date: 2011-04-05
• [Presentation] Tumor microenvironment and prostate cancer : Effects of adipocytes on prostate cancer development and progression (2011)
  • Author(s): M. Watanabe, D. Kurioka, Y. Hamanaka, H. Ishiguro, H. Uemura, Y. Kubota, T. Shiraishi
  • Organizer: An AACR International Conference on New Horizons in Cancer Research
  • Place of Presentation: Delhi, India
• [Presentation] ヒト前立腺癌細胞の3次元培養における抗癌剤抵抗性の獲得機構について (2011)
  • Author(s): 渡邉昌俊,広川佳史,白石泰三
  • Organizer: 第100回日本病理学会総会
  • Place of Presentation: パシフィコ横浜,横浜
• [Presentation] 1炭素代謝に関わる遺伝子多型と前立腺癌のリスクについて (2010)
  • Author(s): 遮邉畳俊, 広川佳史, 加藤貴彦, 鈴木啓悦, 市川智彦, 杉村芳樹, 白石泰三
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2010-09-23
• [Presentation] 前立腺がんスフェロイド形成にかかわる遺伝子の解析 (2010)
  • Author(s): 米田操, 逡邉.昌俊, 高木陽光, 広川佳史, 白石泰三
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2010-09-22
• [Presentation] 前立腺癌における1炭素単位転移機構関連酵素遺伝子多型の解析 (2010)
  • Author(s): 渡邉昌俊,中野洋,村田哲也,白石泰三
  • Organizer: 第57回日本臨床検査医学会学術集会
  • Place of Presentation: 京王プラザホテル,東京
  • Year and Date: 2010-09-11
• [Presentation] 前立腺癌における1炭素単位転移機構関連酵素遺伝子多型の解析 (2010)
  • Author(s): 逡邉昌俊, 中野洋, 村田哲也, 白石泰三.
  • Organizer: 第57回日本臨床検査医学会学術集会
  • Place of Presentation: 京王プラザホテル
  • Year and Date: 2010-09-11
• [Presentation] 前立腺がんスフェロイド形成にかかわる遺伝子の解析 (2010)
  • Author(s): 米田操,渡邉昌俊,高木陽光,広川佳史,白石泰三
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪国際会議場,大阪
• [Presentation] 1炭素代謝に関わる遺伝子多型と前立腺癌のリスクについて (2010)
  • Author(s): 渡邉昌俊,広川佳史,加藤貴彦,鈴木啓悦,市川智彦,杉村芳樹,白石泰三
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪国際会議場,大阪
• [Presentation] 前立腺癌スフェロイドの解析 (2009)
  • Author(s): 森田城次, 上大介, 米田操, 広川佳史, 白石泰三, 渡邉昌俊
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-10-02
• [Presentation] 前立腺癌スフェロイドの解析 (2009)
  • Author(s): 森田城次,上大介,米田操,広川佳史,白石泰三,渡邉昌俊
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜,横浜
• [Presentation] ヒト前立腺癌細胞の3次元培養における抗癌剤抵抗性の獲得とその機構について (2009)
  • Author(s): 渡邉昌俊,上大介,広川佳史,白石泰三
  • Organizer: 第98回に本病理学会総会
  • Place of Presentation: 京都国際会館,京都
• [Presentation] ヒト前立腺癌細胞と脂肪細胞との相互作用について (2009)
  • Author(s): 上大介,渡邉昌俊,長嶋洋治,青木一郎
  • Organizer: 第98回に本病理学会総会
  • Place of Presentation: 京都国際会館,京都