Applications of a new assay for the function of mammalian sperm centrosome
Project/Area Number |
21592079
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Urology
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Research Institution | Chiba University |
Principal Investigator |
ITO Chizuru 千葉大学, 大学院・医学研究院, 講師 (80347054)
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Co-Investigator(Kenkyū-buntansha) |
TOSHIMORI Kiyotaka 千葉大学, 大学院・医学研究院, 教授 (20094097)
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Co-Investigator(Renkei-kenkyūsha) |
TOYAM Yoshiro 千葉大学, 大学院・医学研究院, 特任講師 (70009637)
MAEKAWA Mamiko 千葉大学, 大学院・医学研究院, 助教 (20181571)
YAMATOYA Kenji 千葉大学, 大学院・医学研究院, 特任研究員 (80447309)
KAMIMURA Kyoko 千葉大学, 大学院・医学研究院, 技術専門職員 (20422264)
MUTOU Tooru 千葉大学, 大学院・医学研究院, 技術専門職員 (30422265)
|
Project Period (FY) |
2009 – 2011
|
Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
Keywords | アンドロロジー / 不妊症 / 精子中心体 / 精子機能 |
Research Abstract |
ODF2 is a widespread centrosome scaffold component including sperm centrosome. In order to analyze the function of sperm ODF2, we established ODF2 knockout hetero mice and ODF2-EGFP transgenic (TG) mice. ODF2 hetero male mice were infertile due to the functional failure of sperm. We analyzed the association of ODF2 with other centrosome-associated proteins in mature sperm and developing germ cells using ODF2 KO mice and TG mice.
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Report
(4 results)
Research Products
(131 results)
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[Journal Article] GalNAcβ 1,3-linked paragloboside carries the epitope of a sperm maturation-related glycoprotein that is recognized by the monoclonal antibody MC1212011
Author(s)
Katagiri YU, Sato B, Yamatoya K, Taki T, Goto-Inoue N, Setou M, Okita H, Fujimoto J, Ito C, Toshimori K, Kiyokawa N
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Journal Title
Biochem Biophys Res Commun
Volume: 406(3)
Pages: 326-331
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Peer Reviewed
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[Journal Article] Equatorin : identification and characterization of the epitope of the MN9 antibody in the mouse2009
Author(s)
Yamatoya K, Yoshida K, Ito C, Maekawa M, Yanagida M, Takamori K, Ogawa H, Araki Y, Miyado K, Toyama Y, Toshimori K
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Journal Title
Biol Reprod
Volume: 81(5)
Pages: 889-897
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Peer Reviewed
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[Presentation] Tysnd1 deficiency interferes with mouse peroxisomal functions2011
Author(s)
Mizuno Y, Ninomiya Y, Iseki M, Iwasa H, Akita M, Tsukui T, Ito C, Toshimori K, Shimozawa N, Nishimukai M, Hara H, Maeba R, Okazaki T, Horai Y, Watanabe M, Motegi H, Wakana S, Noda T, Kurochkin Igor V, Mizuno Y, Schoenbach C, Okazaki Y
Organizer
第34回日本分子生物学会年会(ポスター)
Place of Presentation
パシフィコ横浜(神奈川県)
Year and Date
2011-12-15
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[Presentation] 精子の質の検査2010
Author(s)
伊藤千鶴
Organizer
第55回日本生殖医学会総会 学術講演会
Place of Presentation
ホテルクレスト徳島 (徳島県) 招待講演
Year and Date
2010-11-12
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[Presentation] Oocyte-activation ability correlates with presence of perinuclear theca substance MN13, whose organization is associated with acrosome formation2010
Author(s)
Ito C, Yamatoya K, Yoshida K, Akutsu H, Yao R, Kyono K, Suzuki-Toyota F, Toyama Y, Maekawa M, Noda T, Toshimori K.
Organizer
11th International Symposium on Spermatology Okinawa 2010 (Poster)
Place of Presentation
Okinawa convention center, Okinawa (Japan)
Year and Date
2010-06-25
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[Presentation] Further characterization of acrosomal protein Equatorin2010
Author(s)
Yamatoya K, Yoshida K, Ito C, Maekawa M, Yanagida M, Araki Y, Miyado K, Toyama Y, Suzuki-Toyoda F, Toshimori K
Organizer
11th International Symposium on Spermatology Okinawa 2010 (Poster)
Place of Presentation
Okinawa convention center, Okinawa (Japan)
Year and Date
2010-06-25
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[Presentation] 排卵後加齢卵子を分別した透明体除去卵の簡単な調整法2010
Author(s)
大和屋健二, 荒木基行, 古瀬諒二, 伊藤千鶴, 吉田恵一, 前川眞見子, 外山芳郎, 鈴木-豊田二美枝, 年森清隆
Organizer
第115回日本解剖学会総会・全国学術集会岩手県民会館
Place of Presentation
岩手医科大学内丸キャンパス(岩手県)
Year and Date
2010-03-28
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[Presentation] グルコース、アルギニン欠失培地によるマウスES細胞より肝芽細胞様細胞への分化誘導法の開発2010
Author(s)
富澤稔, 外山芳郎, 伊藤千鶴, 年森清隆, 岩瀬克郎, 滝口正樹, 税所宏光, 横須賀収, 篠崎文信, 杉山隆夫, 山本重則, 末石眞, 吉田孝宣
Organizer
第9回日本再生医療学会総会
Place of Presentation
広島国際会議場(広島県)
Year and Date
2010-03-18
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[Presentation] 精子CD9;局在と運命2009
Author(s)
伊藤千鶴, 吉田恵一, 大和屋健二, 前川眞見子, 宮戸健二, 外山芳郎, 鈴木-豊田二美枝, 年森清隆
Organizer
第65回日本顕微鏡学会学術講演会
Place of Presentation
仙台国際センター(宮城県)
Year and Date
2009-05-27
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[Book] 卵子学2011
Author(s)
年森清隆、伊藤千鶴
Total Pages
1210
Publisher
京都大学学術出版
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