Analyzing how defects of Mitochondria DNA affect oocyte maturation.

• Project/Area Number: 21592117
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Osaka Medical College
• Principal Investigator: YAMASHITA Yoshiki 大阪医科大学, 医学部, 講師 (50268207)
• Co-Investigator(Kenkyū-buntansha): OHMICHI Masahide 大阪医科大学, 医学部, 教授 (10283764) ; TERAI Yoshito 大阪医科大学, 医学部, 講師 (90278531) ; TANABE Akiko 大阪医科大学, 医学部, 助教 (70454543) ; HAYASHI Atushi 大阪医科大学, 医学部, 助教 (80411338)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 生殖医学 / ミトコンドリア / 不妊症 / 顆粒膜細胞 / 抗ミュラー管ホルモン(AMH) / AMHレセプタ / 一塩基多型(SNP) / mtDNA4677 / real time PCR / 妊馬血ゴナドトロピン / ラットの顆粒膜細胞 / IVF-ET / hMG / recFSH

Research Abstract

SNP of AMH rather than mitochondria DNA defect might affect the infertility caused by the older age. The influence of AMH-SNP on infertility was not apparent, however, SNP of AMHR(AMHRII-482A> G、、ISV 5-6C> T, ISV10+77A> G) might cause infertility due to ovarian failure involving lower number of retrieved oocyte and fertilization rate. Especially,-482A> G polymorphysm is thought to be associated with diminished ovarian failure.

Research Products

• [Journal Article] MTX could be First-Line Therapy Even in Cases where hCG Level is Greater than 5,000 IU/ml (2012)
  • Author(s): Yoshiki Yamashita, Sousuke Katoh, Yoko Yoshida, Satoe Fujiwara, Sachiko Kawabe, Mika Hayashi, Atsushi Hayashi, Yoshito Terai, Masahide Ohmichi
  • Journal Title: Ectopic pregnancy Modern Diagnosis and Management Pages: 209-216
  • Peer Reviewed
• [Journal Article] Does advanced-stage endometriosis affect the gene expression of estrogen and progesterone receptors in granulose cells? (2011)
  • Author(s): Karita M, Yamashita Y, Hayashi A, Yoshida Y, Hayashi M, Yamamoto H, Tanabe A, Terai Y and Ohmichi M.
  • Journal Title: Fertil Steril Volume: 95 Pages: 889-894
• [Journal Article] Impact of platinum-based chemotherapy on the progression of atherosclerosis (2011)
  • Author(s): Sekijima T, Tanabe A, . Maruoka R, Fujishiro N, Fujiwara S, Yuguchi Y, Yamashita Y, Terai Y and Ohmichi M.
  • Journal Title: Climacteric Volume: 14 Pages: 31-40
• [Journal Article] Prognostic effect of epidermal growth factor receptor gene mutations and the aberrant phosphorylation of Akt and ERK in ovarian cancer (2011)
  • Author(s): Yoshimichi Tanaka, Yoshito Terai, Akiko Tanabe, Hiroshi Sasaki, Tatsuharu Sekijima, Satoe Fujiwara, Yoshiki Yamashita, Masanori Kanemura, Masatsugu Ueda, Michio Sugita, Wilbur A. Franklin and Masahide Ohmichi
  • Journal Title: Cancer Biol Ther Volume: 11 Pages: 50-57
• [Journal Article] Requirement of evading apoptosis for HIF-1α-induced malignant progression in mouse cells (2011)
  • Author(s): Hayashi M, Yoo YY, Christensen J, Huang LE
  • Journal Title: Cell Cycle Volume: 10 Pages: 2364-2372
• [Journal Article] MTX could be First-Line Therapy Even in Cases where hCG Level is Greater than 5,000 IU/ml (2011)
  • Author(s): Yoshiki Yamashita, Sousuke Katoh, Yoko Yoshida, Satoe Fujiwara, Sachiko Kawabe, Mika Hayashi, Atsushi Hayashi, Yoshito Terai and Masahide Ohmichi
  • Journal Title: Ectopic Pregnancy-Modern Diagnosis and Management.
• [Journal Article] Does advanced-stage endometriosis affect the gene expression of estrogen and progesterone receptors in granulose cells? (2011)
  • Author(s): Karita M, Yamashita Y, Hayashi A, Yoshida Y, Hayashi M, Yamamoto H, Tanabe A, Terai Y, Ohmichi M
  • Journal Title: Fertility and Sterility Volume: 95 Pages: 889-894
  • Peer Reviewed
• [Journal Article] Remarkable Lethal G-to-A Mutations in vif-Proficient HIV-1 Provirus by Individual APOBEC3 Proteins in Humanized Mice (2010)
  • Author(s): Kei Sato, Taisuke Izumi, Naoko Misawa, Tomoko Kobayashi, Yoshiki Yamashita, Masahide Ohmichi, Mamoru Ito, Akifumi Takaori-Kondo, and Yshio Koyanagi
  • Journal Title: J Virol Volume: 4 Pages: 9546-9556
• [Journal Article] Topotecan as a molecular targeting agent which blocks the Akt and VEGF cascade in platinum-resistant ovarian cancers (2010)
  • Author(s): Tsunetoh S, Terai Y, Sasaki H, Tanabe A, Tanaka Y, Sekijima T, Fujioka S, Kawaguchi H, Kanemura M, Yamashita Y, Ohmichi M
  • Journal Title: Cancer Biol Ther Volume: 10 Pages: 1137-114
• [Journal Article] A Case of Concomitant Pregnancy Following Supplement of Mestranol and Norethisterone to Control the Menstrual Cycle (2010)
  • Author(s): Tomohito Tanaka, Yoshiki Yamashita, Sousuke Kato, Takashi Kato, Yoshito Terai and Masahide Ohmichi
  • Journal Title: Bull OMC Volume: 56 Pages: 91-94
• [Journal Article] Mature cystic teratoma of the fallopian tube (2010)
  • Author(s): Satoe Fujiwara, Yoshiki Yamashita, Yoko Yoshida, Yoshito Terai, Kiyoji Okuda, Masahide Ohmichi
  • Journal Title: Fertil Steril Volume: 92 Pages: 2708-2709
• [Journal Article] Vascular endothelial growth factor is a promising therapeutic target for the treatment of clear cell carcinoma of the ovary (2010)
  • Author(s): Mabuchi S, Kawase C, Altomare DA, Morishige K, Hayashi M, Sawada K, Ito K, Terai Y, Nishio Y, Klein-Szanto AJ, Burger RA, Ohmichi M, Testa JR, Kimura T.
  • Journal Title: Mol Cancer Ther Volume: 9 Pages: 2411-2422
• [Journal Article] Effectiveness of laparoscopic surgery against antibiotics-resistant pelvic inflammatory disease (2009)
  • Author(s): Yamashita Y., Okuda K., Hayashi A., Terai Y., Ohmichi M.
  • Journal Title: Bull of OMC Volume: 15 Pages: 1-7
  • NAID: 130001223243
• [Journal Article] A case of an MTX-resistant ectopic pregnancy where Dactinomycin was effective as a second-linechemotherapy (2009)
  • Author(s): Fujioka S., Yamashita Y., Asano M., Hayashi A., Ohmichi M
  • Journal Title: Fertil Steril Volume: 91
• [Journal Article] Long-term follow-up of neoadjuvant intraarterial chemotherapy using an original four-lumen double-balloon(4L-DB) catheter for locally advanced uterine cervical cancer (2009)
  • Author(s): Terai Y, Kanemura M, Sasaki H, Tsunetoh S, Tanaka Y, Yamashita Y, Yamamoto K, Sasaki H, Yamashita Y, Kanemura, Ohmichi M.
  • Journal Title: M, Int J Clin Oncol Volume: 14 Pages: 56-62
  • NAID: 10025147392
• [Journal Article] Successful pregnancy after conservative surgery for stage IA endometrial cancer in a young woman (2009)
  • Author(s): Yasuda M, Terai Y, Sekijima T, Sasaki H, Yamashita Y, Kanemura M, Ohmichi M
  • Journal Title: Fertil Steril Volume: 91
• [Journal Article] A case of a methotrexate-resistant ectopic pregnancy in which dactinomycin was effective as a second-line chemotherapy (2009)
  • Author(s): Fujioka S, Yamashita Y, Kawabe S, Kamegai H, Terai Y, Ohmichi M
  • Journal Title: Fertil Steril Volume: 91
• [Journal Article] mTOR is a promising therapeutic target both in cisplatin-sensitive and cisplatin-resistant clear cell carcinoma of the ovary (2009)
  • Author(s): Mabuchi S, Kawase C, Altomare DA, Morishige K, Sawada K, Hayashi M, Tsujimoto M, Yamoto M, Klein-Szanto AJ, Schilder RJ, Ohmichi M, Testa J R, Kimura T.
  • Journal Title: Clin Cancer Res Volume: 15 Pages: 5404-5413
• [Presentation] 体外受精治療下黄体化顆粒膜細胞におけるBMP15,GDF9 mRNAの発現の臨床的検討 (2012)
  • Author(s): 川辺紗智子、吉田陽子、山下能毅、林篤史、林美佳、林正美、大道正英
  • Organizer: 日本産婦人科学会
  • Place of Presentation: 神戸
  • Year and Date: 2012-04-14
• [Presentation] 子宮内膜症合併不妊症の黄体化顆粒膜細胞におけるAMHおよびAMHR mRNA発現への影響 (2012)
  • Organizer: 日本産婦人科学会
  • Place of Presentation: 神戸
• [Presentation] 体外受精治療下黄体化顆粒膜細胞におけるBMP15, GDF9 mRNAの発現の臨床的検討 (2012)
  • Organizer: 日本産婦人科学会
  • Place of Presentation: 神戸
• [Presentation] 子宮内膜症症例の顆粒膜細胞におけるアンドロゲンレセプター(AR)の発現とメチル化の有無の検討 (2011)
  • Author(s): 林美佳、川辺紗智子、吉田陽子、山下能毅、林篤史、林美佳、林正美、大道正英
  • Organizer: 生殖医学会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-08
• [Presentation] 子宮内膜症症例の顆粒膜細胞におけるアンドロゲンレセプター(AR)の発現とメチル化の有無の検討 (2011)
  • Organizer: 生殖医学学会
  • Place of Presentation: 横浜