Protection of the Retina by Rapid Diffusion of Hydrogen : Administration of Hydrogen-Loaded Eye Drops in Retinal Ischemia. Reperfusion Injury

• Project/Area Number: 21592248
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Nippon Medical School
• Principal Investigator: OHARAZAWA Hideaki 日本医科大学, 医学部, 准教授 (20350035)
• Co-Investigator(Kenkyū-buntansha): 大澤 郁朗 日本医科大学, 老人病研究所, 教授 (30343586)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 網膜虚血-再灌 / 水素分子 / 網膜神経保護 / 酸化ストレス / SNAP / peroxynitrite / ex vivo / 水素点眼 / 活性酸素種 / 網膜虚血-再灌流傷害

Research Abstract

This study was conducted to explore the neuroprotective effect of H_2-loaded eye drops on retinal ischemia-reperfusion(I/R) injury in rats. H_2-loaded eye drops reduced the number of retinal apoptotic and oxidative stress marker. positive cells and prevented retinal thinning with an accompanying activation of Muller glia, astrocytes, and microglia. The results suggest that H_2-loaded eye drops are a highly useful neuroprotective and antioxidative therapeutic treatment for acute retinal I/R injury.

Research Products

• [Journal Article] Protection of the retina by rapid diffusion of hydrogen : Administration of hydrogen-loaded eye drops inretinal ischemia-reperfusion injury (2010)
  • Author(s): Oharazawa, H., Igarashi, T., Yokota, T., Fujii, H., Suzuki, H., Machide, M., Takahashi, H., Ohta, S. and Ohsawa, I
  • Journal Title: Invest Ophthalmol Vis Sci. Volume: 51(1) Issue: 1 Pages: 487-492
  • DOI: 10.1167/iovs.09-4089
  • Peer Reviewed
• [Journal Article] Protection of the retina by rapid diffusion of hydrogen : Administration of hydrogen-loaded eye drops inretinal ischemia-reperfusion injury. (2010)
  • Author(s): 小原澤英彰
  • Journal Title: Investigative Ophthalmology & Visual Sciences Vol.51 Pages: 847-892
  • Peer Reviewed
• [Presentation] Rapid diffusion of hydrogen molecule from hydrogen-containing eye drops reduces retinal ischemia-reperfusion injury (2010)
  • Author(s): Oharazawa, H., Igarashi, T., Yokota, T., Takahashi, H., Ohta, S., Ohsawa, I.
  • Organizer: 7^<th> ASMRM・10^<th> J-mit
  • Place of Presentation: Fukuoka
  • Year and Date: 2010-12-16
• [Presentation] ラット網膜虚血再灌流モデルにおける水素点眼による神経保護効果 (2010)
  • Author(s): 小原澤英彰五十嵐勉横田隆藤井博明鈴木久晴町出充高橋浩太田成男大澤郁朗
  • Organizer: 第114回日本眼科学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2010-04-16
• [Presentation] ラット網膜虚血-再還流モデルにおける水素点眼の網膜神経保護効果 (2010)
  • Author(s): 小原澤英彰
  • Organizer: 第114回日本眼科学会総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2010-04-16
• [Presentation] Rapid diffusion of hydrogen protects the retina : Administration to the eye of hydrogen-containing saline in retinal ischemia-reperfusion injury (2010)
  • Author(s): Oharazawa, H., Igarashi, T., Yokota, T., Fujii, H., Suzuki, H., Machide, M., Takahashi, H., Ohta, S., Ohsawa, I.
  • Organizer: OCC 2010 World Congress
  • Place of Presentation: Santa Barbara
  • Year and Date: 2010-03-17
• [Presentation] Rapid diffusion of hydrogen protects the retina : Administration to the eye of hydrogen-containing saline in retinal ischemia-reperfusion injury. (2010)
  • Author(s): 小原澤英彰
  • Organizer: Oxygen Club of California 2010 World Congress
  • Place of Presentation: サンタバーバラ
  • Year and Date: 2010-03-17