Roles of macrophages in the development of allergic conjunctival diseases

• Project/Area Number: 21592259
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Kochi University
• Principal Investigator: FUKUSHIMA Atsuki 高知大学, 教育研究部・医療学系, 教授 (40281737)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: アレルギー / 結膜 / 抗原提示細胞 / FcεRIα / 好塩基球 / T細胞 / マクロファージ / 好酸球 / 免疫染色 / 電子顕微鏡

Research Abstract

Vernal keratoconjunctivitis(VKC) is a severe form of allergic conjunctival diseases and may lead to blindness. VKC is an antigen-specific disease and antigen-presenting cels play crucial roles in its development. During this research period, we found that basophils are exerting antigen-presenting capability in allergic conjunctival diseases.

Research Products

• [Journal Article] Adjuvants determine the contribution of basophils to antigen sensitization in vivo (2011)
  • Author(s): Ishida W, Fukuda K, Sumi T, Ebihara N, Kajisako M, Matsuda H, Yagita H, Fukushima A
  • Journal Title: Immunol Lett Volume: 136 Pages: 49-54
  • Peer Reviewed
• [Journal Article] Participation of CD11b and F4/ 80 molecules in the conjunctival eosinophilia of experimental allergic conjunctivitis (2010)
  • Author(s): Fukushima A, Ishida W, Ojima A, Kajisako M, Sumi T, Yamada J, Tsuru E, Miyazaki J-i, Tominaga A, Yagita H
  • Journal Title: Int Arch Allergy Immunol Volume: 151(2) Pages: 129-136
  • Peer Reviewed
• [Journal Article] Conjunctival macrophages act as antigen-presenting cells in the conjunctiva during the development of experimental allergic conjunctivitis (2010)
  • Author(s): Ishida W, Kajisako M, Takahashi A, Sumi T, van Rooijen N, Fukuda K, Fukushima A
  • Journal Title: Mol Vision Volume: 16 Pages: 1280-1285
  • Peer Reviewed
• [Journal Article] Participation of CD11b and F4/80 molecules in the conjunctival eosinophilia of experimental allergic conjunctivitis. (2010)
  • Author(s): Fukushima A, Ishida W, Ojima A, Kajisako M, Sumi T, Yamada J, Tsuru E, Miyazaki J-i, Tominaga A, Yagita H.
  • Journal Title: Int.Arch.Allergy.Immunol. Volume: 151 Pages: 129-136
  • Peer Reviewed
• [Journal Article] A randomised, placebo-controlled clinical trial of tacrolimus ophthalmic suspension 0.1% in severe allergic conjunctivitis. (2010)
  • Author(s): Ohashi Y, Ebihara N, Fujishima H, Fukushima A, Kumagai N, Nakagawa Y, Nanba K, Okamoto S, Shoji J, Takamura E, Hayashi K
  • Journal Title: J.Ocul.Pharmacol.Ther. Volume: 26 Pages: 165-174
  • Peer Reviewed
• [Journal Article] T cell-related co-stimulatory molecules in the conjunctiva of patients with severe allergic conjunctivitis. (2010)
  • Author(s): Sumi T, Ishida W.Ebihara N, Fukushima A.
  • Journal Title: Cornea Volume: 29 Pages: 622-627
  • Peer Reviewed
• [Journal Article] Aggravation of conjunctival early-phase reaction by staphylococcus aureus enterotoxin B via augmentation of IgE production. (2010)
  • Author(s): Miyazaki D, Ishida W, Tominaga T, Sumi T, Fukushima A
  • Journal Title: Jpn J Ophthalmol Volume: 54 Pages: 476-480
  • Peer Reviewed
• [Journal Article] Evaluation of histamine-induced conjunctival edema in guinea-pigs using image analysis. (2010)
  • Author(s): Takahashi A, Sumi T, Tada K, Mibu, Shii D, Kayasuga A, Fukushima A.
  • Journal Title: Br J Ophthalmol Volume: 94 Pages: 1657-1661
  • Peer Reviewed
• [Journal Article] Conjunctival macrophages act as antigen-presenting cells in the conjunctiva during the development of experimental allergic conjunctivitis. (2010)
  • Author(s): Ishida W, Kajisako M, Takahashi A, Sumi T, van Rooijen N, Fukuda K, Fukushima A
  • Journal Title: Mol Vision Volume: 16 Pages: 1280-1285
  • Peer Reviewed
• [Journal Article] Activation of tumor necrosis factor-related apoptosis-inducing ligand receptor enhances the severity of murine allergic conjunctivitis (2009)
  • Author(s): Sumi T, Ishida W, Okumura K, Yagita H, Fukushima A.
  • Journal Title: Br J Ophthalmol 93 Pages: 110-115
  • Peer Reviewed
• [Journal Article] Image analyses of the kinetic changes of conjunctival hyperemia in histamine-induced conjunctivitis in guinea pigs (2009)
  • Author(s): Fukushima A, Tomita T.
  • Journal Title: Cornea 29 Pages: 694-698
  • Peer Reviewed
• [Journal Article] Systemic overexpression of IFN-γ and IL-5 exacerbates early phase reaction and conjunctival eosinophilia, respectively, in experimental all ergic conjunctivitis (2009)
  • Author(s): Ishida W, Tsuru E, Tominaga A, Miyazaki J-i, Higuchi T, Sakamoto S, Fukushima A.
  • Journal Title: Br J Ophthalmol 93 Pages: 1680-1685
  • Peer Reviewed
• [Journal Article] Treatment with FTY720 during the induction or effector phase suppresses the development of experimental allergic conjunctivitis in mice (2009)
  • Author(s): Sumi T, Fukushima A, Nishino K, Fukuda K, Kumagai N, Nishida T, Ueno H.
  • Journal Title: Cell Biol Int 33 Pages: 534-541
  • Peer Reviewed
• [Journal Article] MHC-matched corneal allograft rejection in an IFN-gamma/IL-17-independent manner in C57BL/6 mice (2009)
  • Author(s): Yamada J, Hamuro J, Fukushima A, Ohteki T, Term K, Iwakura Y, Yagita H, Kinoshita S.
  • Journal Title: Invest Ophthalmol Vis Sci. 50 Pages: 2139-2146
  • Peer Reviewed
• [Journal Article] Ablation of type I hypersensitivity in experimental allergic conjunctivitis by eotaxin-1/CCR3 blockade (2009)
  • Author(s): Miyazaki D, Nakamura T, Ohbayashi M, Kuo CH, Komatsu N, Yakura K, Tominaga T, Inoue Y, Higashi H, Murata M, Takeda S, Fukushima A, Liu FT, Rothenberg ME, Ono SJ.
  • Journal Title: Int Immunol.l 21 Pages: 187-201
  • NAID: 10025384437
  • Peer Reviewed
• [Journal Article] Activation of tumor necrosis factor-related apoptosis-inducing ligand receptor enhances the severity of murine allergic conjunctivitis (2009)
  • Author(s): Fujishima H, Fukagawa K, Takano Y, Tanaka M, Okamoto S, Miyazaki D, Yamada J, Fukushima A, Uchio E.
  • Journal Title: J Ocul Pharmacol Ther 25 Pages: 265-270
  • Peer Reviewed
• [Journal Article] A large prospective observational study of novel cyclosporine 0.1% aqueous ophthalmic solution in thetreatment of severe allergic conjunctivitis (2009)
  • Author(s): Ebihara N, Ohashi Y, Uchio E, Okamoto S, Kumagai N, Shoji J, Takamura E, Nakagawa Y, Nanba K, Fukushima A, Fujishima H.
  • Journal Title: J Ocul Pharinacol Ther 25 Pages: 365-372
  • Peer Reviewed
• [Journal Article] The NF90-NF45 complex functions as a negative regulator in the microRNA processing pathway (2009)
  • Author(s): Sakamoto S, Aoki K, Higuchi T, Todaka H, Morisawa K, Tamaki N, Hatano E, Fukushima A, Taniguchi T, Agata Y.
  • Journal Title: Mol Cell Biol 29 Pages: 3754-3769
  • NAID: 120001756537
  • Peer Reviewed
• [Journal Article] Image analyses of conjunctival hyperemia in guinea pig allergic conjunctivitis (2009)
  • Author(s): Fukushima A, Tomita T.
  • Journal Title: Graefe's Arch Clin Exp Ophthalmol 247 Pages: 1571-1572
  • Peer Reviewed
• [Presentation] Severe allergic keratoconjunctivitis (2010)
  • Author(s): Atsuki Fukushima
  • Organizer: The 2^<nd> Asia Cornea Society Biennial Scientific Meeting
  • Place of Presentation: ウェスティン都ホテル京都
  • Year and Date: 2010-12-03
• [Presentation] アレルギー性結膜炎モデルを用いた病態の解析 (2009)
  • Author(s): 福島敦樹
  • Organizer: 第59回日本アレルギー学会秋季学術大会 ワークショップ
  • Place of Presentation: 秋田ビューホテル
  • Year and Date: 2009-10-29
• [Book] 眼のサイエンス 眼疾患の謎.涙液にあるラクトフェリンやリゾチームはどんな働きをしているのか? (2010)
  • Author(s): 福島敦樹
  • Publisher: 文光堂