Study of evolution of osteoclasts regulating factors in teleost fish

• Project/Area Number: 21592319
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Hokkaido University
• Principal Investigator: DOMON Takanori 北海道大学, 大学院・歯学研究科, 教授 (50217618)
• Co-Investigator(Kenkyū-buntansha): YOSHIMURA Yoshitaka 北海道大学, 大学院・歯学研究科, 助教 (30230816)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 口腔解剖学(含組織学・発生学) / 破骨細胞 / 硬骨魚類 / メダカ / 分離培養 / 走査型電子顕微鏡 / 透過型電子顕微鏡 / TRAP陽性 / TRAP陽性細胞 / 咽頭歯 / 咽頭歯骨

Research Abstract

The origin of osteoclasts resorbing bone remains unknown. Bone tissue is seen on teleost fish in phylogeny, and there old osteoclasts would be seen. The present study established the culture system of isolated Japanese medaka and observed their structure by light-and electron microscopy. The cultured cells showed no resorptive lacuna but etched surfaces on sperm whale dentine. These results may be related to the evolution of regulating fac tors of osteoclasts.

Research Products

• [Journal Article] Dental pulp can be a good candidate for nerve grafting in a xeno-graft model (2012)
  • Author(s): Matsushita K, Wang W, Itoh S, Domon T, Funahashi M, Totsuka Y.
  • Journal Title: J Nuerosci Method Volume: 205 Issue: 2 Pages: 246-251
  • DOI: 10.1016/j.jneumeth.2011.12.026
  • NAID: 120004027243
  • Peer Reviewed
• [Journal Article] Platinum Nanoparticles Suppress Osteoclastogenesis Through Scavenging of Reactive Oxygen Species Produced in RAW264.7 Cells (2011)
  • Author(s): Nomura M, Yoshimura Y, Kikuiri T, Hasegawa T, Taniguchi Y, Deyama Y, Koshiro K, Sano H, Suzuki K, Inoue N.
  • Journal Title: Journal of Pharmacological Sciences Volume: 117 Issue: 4 Pages: 243-252
  • DOI: 10.1254/jphs.11099FP
  • NAID: 10030453660
  • ISSN: 1347-8613, 1347-8648
  • Peer Reviewed
• [Journal Article] Effect of the release from mechanical stress on osteoclastogenesis in RAW 264. 7 cells (2011)
  • Author(s): Shibata K, Yoshimura Y, Kikuiri T, Hasegawa T, Taniguchi Y, Deyama Y, Suzuki K, Iida J.
  • Journal Title: Int J Molecular Medicin Volume: 28 Pages: 73-79
  • DOI: 10.3892/ijmm.2011.675
  • Peer Reviewed
• [Journal Article] Initial process of enamel prism arrangement and its relation to the Hunter-Schreger bands in dog teeth (2010)
  • Author(s): Hanaizumi Y, Yokota R, Domon T, Wakita M, Kozawa Y.
  • Journal Title: Arch Histol Cytol Volume: 73 Pages: 23-26
  • NAID: 10028159953
  • URL: https://www.jstage.jst.go.jp/browse/aohc
  • Peer Reviewed
• [Journal Article] Educational effects in the general education course of Hokkaido University "Science of saliva" using the audience response system, clicker (2010)
  • Author(s): Takahashi S, Inoue K, Funahashi M, Domon T.
  • Journal Title: Hokkaido J Dent Sci Volume: 31 Pages: 75-31
  • NAID: 10028208608
  • URL: http://eprints.lib.hokudai.ac.jp/journals/index.php?jname=318
  • Peer Reviewed
• [Journal Article] Effect of estrogen on PMCA 2 and 4 in human fibroblast-like synovial cells and mouse macrophage-like cells (2010)
  • Author(s): EL-Beillard N, Galal N, Deyama Y, Yoshimura Y, Suzuki K, Tei K, Totsuka Y.
  • Journal Title: Endocrine J Volume: 57 Pages: 93-97
  • NAID: 10029583516
  • URL: http://mol.medicalonline.jp/library/archive/select?jo=cq6endoc
  • Peer Reviewed
• [Journal Article] Initial process of enamel prism arrangement and its relation to the Hunter-Schreger bands in dog teeth. (2010)
  • Author(s): Hanaizumi Y, Yokota R, Domon T, Wakita M, Kozawa Y.
  • Journal Title: Archives of Histology and Cytology Volume: 73 Pages: 23-36
  • NAID: 10028159953
  • Peer Reviewed
• [Journal Article] Effect of the release from mechanical stress on osteoclastogenesis in RAW264.7 cells. (2010)
  • Author(s): Shibata K, Yoshimura Y, Kikuiri T, Hoasgawa T, Taniguchi Y, Deyama Y, Suzuki K, Iida J.
  • Journal Title: International Journal of Molecular Medicine Volume: (印刷中,印刷中)
  • Peer Reviewed
• [Journal Article] Type XVII collagen is a key player in tooth enamel formation (2009)
  • Author(s): Asaka T, Akiyama M, Domon T, Nishie M, Natsuga K, Fujita Y, Abe R, Kitagawa Y, Shimizu H.
  • Journal Title: Am J Pathol Volume: 171 Issue: 1 Pages: 91-100
  • DOI: 10.2353/ajpath.2009.080573
  • Peer Reviewed
• [Journal Article] Mechanical stress up-regulates RANKL expression via the VEGF autocrine pathway in osteo-blastic MC3T3-E1 cells (2009)
  • Author(s): Nakai T, Yoshimura Y, Deyama Y, Suzuki K, Iida J.
  • Journal Title: Molecular Medicine Reports Volume: 2 Pages: 229-234
  • DOI: 10.3892/mmr_00000088
  • Peer Reviewed
• [Journal Article] Effect of mineral trioxide aggregate on rat clonal dental pulp cells : expression of cyclooxygenase-2 mRNA and inflammation-released protein via nuclear factor Kappa B signaling system (2009)
  • Author(s): Minamikawa H, Deyama Y, Nakamura K, Yoshimura Y, Kaga M, Suzuki K, Yawaka Y.
  • Journal Title: J Endodontics Volume: 35 Issue: 6 Pages: 834-846
  • DOI: 10.1016/j.joen.2009.03.008
  • Peer Reviewed
• [Journal Article] Up-regulation of the G3PDH housekeeping gene by estrogen (2009)
  • Author(s): Galal N, El-Beialy W, Deyama Y, Yoshimura Y, Tei K, Suzuki K, Totsuka Y.
  • Journal Title: Molecular Medicine reports Volume: 2 Issue: 1 Pages: 111-114
  • DOI: 10.3892/mmr_00000226
  • Peer Reviewed
• [Journal Article] Type XVII collagen is a key player in tooth enamel formation. (2009)
  • Author(s): Asaka T, Akiyama M, Domon T, Nishie W, Natsuga K, Fujita Y, Abe R, Kitagawa Y, Shimizu H
  • Journal Title: American Journal of Pathology 174 Pages: 91-100
  • Peer Reviewed
• [Journal Article] 骨芽細胞葉細胞のMg^<2+>依存性ATPase活性の性質 (2009)
  • Author(s): 齋藤豪紀、出山義昭、吉村善隆、戸塚靖則、鈴木邦明
  • Journal Title: 北海道歯学雑誌 30 Pages: 61-68
  • Peer Reviewed
• [Presentation] マウス中足骨に見られる前破骨細胞の三次元的特徴 (2011)
  • Author(s): 土門卓文
  • Organizer: 日本解剖学会第57回東北・北海道連合支部学術集会
  • Place of Presentation: 岩手大学教育学部講堂(盛岡)
  • Year and Date: 2011-09-10
• [Presentation] マウス中足骨に見られる前破骨細胞の三次元的特徴 (2011)
  • Author(s): 土門卓文
  • Organizer: 日本解剖学会第57回東北・北海道連合支部学術集会
  • Place of Presentation: 盛岡市、岩手大学教育学部講堂
  • Year and Date: 2011-09-10
• [Presentation] The reason of multinucleation of osteoclast-A Personal Perspective (2011)
  • Author(s): Domon T
  • Organizer: 平成20年度日本大学松戸歯学部への私立大学戦略的研究基盤支援事業(2008-2012)歯の形態形成研究班発表会
  • Place of Presentation: 東京都千代田区九段南日本犬学会館
  • Year and Date: 2011-02-26
• [Presentation] The reason of multinucleation of osteoclast A Personal Perspective- (2011)
  • Author(s): Domon T
  • Organizer: 平成20年度日本大学松戸歯学部への私立大学戦略的研究基盤支援事業(2008-2012)歯の形態形成研究班発表会
  • Place of Presentation: 日大会館(東京)
  • Year and Date: 2011-02-25
• [Presentation] 生理的歯根吸収時における破歯細胞死の微細構造 (2010)
  • Author(s): 土門卓文
  • Organizer: 日本解剖学会第56回東北・北海道連合支部学術集会
  • Place of Presentation: 旭川市旭川医科大学医学部講堂
  • Year and Date: 2010-09-25
• [Presentation] 脊椎動物の顎・顎関節の進化とヒト耳小骨との関わり (2010)
  • Author(s): 土門卓文
  • Organizer: 滝川市三師会学術講演会
  • Place of Presentation: ホテル末広(滝川)
  • Year and Date: 2010-09-17
• [Presentation] 解剖学の教育と研究から学んだこと (2010)
  • Author(s): 土門卓文
  • Organizer: 北海道大学歯学部同窓会平成22年度第2回札幌支部学術講演会
  • Place of Presentation: ホテルサンルート札幌(札幌)
  • Year and Date: 2010-05-08
• [Presentation] 脊椎動物の顎の進化ヤツメウナギからヒトまで (2009)
  • Author(s): 土門卓文
  • Organizer: 北海道大学大学院歯学研究科・歯学部市民公開講座
  • Place of Presentation: 北海道大学学術交流会館(札幌)
  • Year and Date: 2009-09-27
• [Presentation] 破骨細胞の多核の理由は何か (2009)
  • Author(s): 土門卓文
  • Organizer: 平成21年度北海道歯学会秋期学術大会
  • Place of Presentation: 北海道大学歯学部講堂(札幌)
  • Year and Date: 2009-09-24
• [Presentation] 破骨細胞の多核の理由は何か (2009)
  • Author(s): 土門卓文
  • Organizer: 平成21年度北海道歯学会秋期学術大会
  • Place of Presentation: 札幌市 北海道大学歯学部講堂
  • Year and Date: 2009-09-24
• [Presentation] ヒト乳歯歯根膜由来線維芽細胞からの血管新生誘導 (2009)
  • Author(s): 長谷川智一、帖左直幸、浅川剛吉、吉村善隆、石崎明、田中光郎
  • Organizer: 第47回日本小児歯科学会大会
  • Place of Presentation: 吹田市 大阪大学コンベンションセンター
  • Year and Date: 2009-05-15
• [Presentation] Apoptpsis of odontoclasts and osteoclasts under physiological conditions (2009)
  • Author(s): Domon T
  • Organizer: 平成20年度私立大学戦略的研究基盤支援事業研究集会
  • Place of Presentation: 日大会館(東京)
  • Year and Date: 2009-02-20
• [Presentation] 生理的歯根吸収時における破歯細胞の細胞死の微細構造
  • Author(s): 土門卓文
  • Organizer: 日本解剖学会第56回東北・北海道連合支部学術集会
  • Place of Presentation: 旭川医科大学看護学部講堂(旭川)
• [Book] 頸部、ネッター頭頸部・口腔顎顔面の臨床解剖学アトラス (2012)
  • Author(s): 土門卓文
  • Total Pages: 622
  • Publisher: 医歯薬出版
• [Book] ネックー頭頸部・口腔額顔面の臨床解剖学アトラス、前田健康監訳 (2012)
  • Author(s): 土門卓文(分担)
  • Publisher: 医歯薬出版
• [Book] 骨格系・生殖器、歯科衛生士教本人体の構造と機能 (2010)
  • Author(s): 土門卓文
  • Total Pages: 251
  • Publisher: 医歯薬出版
• [Remarks]
  • URL: http://www.den.hokudai.ac.jp/anatomy1/ana1.html