Role of matrix proteins on the differentiation and activity of hard tissue forming cells and calcification during tooth formation.

• Project/Area Number: 21592329
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Hiroshima University
• Principal Investigator: UCHIDA Takashi 広島大学, 大学院・医歯薬学総合研究科, 教授 (50150305)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 歯の発生 / エナメル蛋白 / アメロブラスチン / 歯根形成 / 細胞増殖 / 細胞分化 / エナメルタンパク / マラッセ上皮遺残 / ヘルトビッヒ上皮鞘 / アメロゲニン / エナメリン / エナメル器 / セメント質 / 象牙質 / 歯胚

Research Abstract

This study clarified that ameloblastin, one of the enamel matrix protein, suppressed mitotic activity and raised expression of amelogenin and enamelin of the ameloblat-linage cell in vitro, and was essential for normal mitosis and differentiation of cells of Hertwig' s epithelial root sheath during root formation in vivo. Moreover, ameloblastin is supposed to be one of the candidate molecules of inducing differentiation of odontoblasts and cementocytes.

Research Products

• [Journal Article] Fine structural and immunohistochemicaldetection of collar enamel in the teeth of Polypterus senegalus, an actinopterygianfish (2012)
  • Author(s): Sasagawa I., Yokosuka H., Ishiyama M., Mikami M., Shimokawa H. Uchida T.
  • Journal Title: Cell Tissue Res. Volume: 347(2) Pages: 369-381
  • Peer Reviewed
• [Journal Article] Histological and immunohistochemicalanalyses of molar tooth germ inenamelin-deficient mouse. (2011)
  • Author(s): Sawada T. Sekiguchi H., Uchida T., Yamashita H., Shintani S., Yanagisawa T.
  • Journal Title: Acta Histochem Volume: 113(5) Pages: 542-546
  • Peer Reviewed
• [Journal Article] Ameloblastin regulates osteogenicdifferentiation by inhibiting Src kinasevia cross talk between integrin beta1 andCD63. (2011)
  • Author(s): Iizuka S., Kudo Y., Yoshida M., Tsunematsu T., Yoshiko Y., Uchida T., Ogawa I., Miyauchi M., Takata T.
  • Journal Title: Mol Cell Biol Volume: 31(4) Pages: 783-792
  • Peer Reviewed
• [Journal Article] Ameloblastin regulates osteogenic differentiation by inhibiting Src kinase via cross talk between integrin beta1 and CD63. (2011)
  • Author(s): Iizuka S, et al.
  • Journal Title: Mol Cell Biol Volume: 31 Pages: 783-792
  • Peer Reviewed
• [Journal Article] Syntheticameloblastin peptide stimulatesdifferentiation of human periodontalligament cells (2010)
  • Author(s): Kitagawa M, Kitagawa S, Nagasaki A, Miyauchi M, Uchida T, Takata T.
  • Journal Title: Arch Oral Biol Volume: 56(4) Pages: 374-379
  • Peer Reviewed