Inhibition of P. gingivalis infection by the SLPI from gingival epithelial cells
Project/Area Number |
21592340
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Morphological basic dentistry
|
Research Institution | Iwate Medical University |
Principal Investigator |
|
Co-Investigator(Kenkyū-buntansha) |
NEMOTO Yuko (NEMOTO OHARA Yuko) 長崎大学, 大学院・医歯薬学総合研究科, 准教授 (10164667)
|
Co-Investigator(Renkei-kenkyūsha) |
SASAKI Minoru 岩手医科大学, 歯学部, 准教授 (40187133)
SHIMOYAMA Yu 岩手医科大学, 歯学部, 助教 (90453331)
|
Project Period (FY) |
2009 – 2011
|
Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2009: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
|
Keywords | SLPI / 歯肉上皮細胞 / P. gingivalis / 感染抑制 / P.gingivalis |
Research Abstract |
The results indicated that gingival epithelial cells could be a substantial producer of secretory leukocyte protease inhibitor(SLPI) that functions inhibitory to the pathogenic Porphyromonas gingivalis protease in gingival crevices. It was also suggested that the SLPI production could increase in response to P. gingivalis through the stimulation with its pathogenic constituents.
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Report
(4 results)
Research Products
(47 results)
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[Journal Article] Proteome analyses of human macrophages exposed to low cytotoxic IC90 copper(2+) ions2011
Author(s)
Taira, M., Shimoyama, Y., Kagiya, T., Sasaki, M., Nezu, T., Harada, H. and Kimura, S.
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Journal Title
Dent. Mater. J.
Volume: 30
Pages: 293-299
NAID
Related Report
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[Journal Article] Asp-and Glu-specific novel dipeptidyl peptidase 11 of Porphyromonas gingivalis that ensures utilization of proteineous energy sources2011
Author(s)
Ohara-Nemoto, Y., Shimoyama, Y., Kimura, S., Kon, A., Haraga, H., Ono, T. and Nemoto, T. K.
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Journal Title
J. Biol. Chem.
Volume: 286
Pages: 38115-38127
NAID
Related Report
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[Journal Article] Asp- and Glu-specific novel dipeptidyl peptidase 11 of Porphyromonas gingivalis that ensures utilization of proteineous energy sources2011
Author(s)
Ohara-Nemoto, Y., Shimoyama, Y., Kimura, S., Kon, A., Haraga, H., Ono, T., Nemoto, T.K.
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Journal Title
J.Biol.Chem.
Volume: 286
Pages: 38115-38127
NAID
Related Report
Peer Reviewed
-
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[Journal Article] Microscopic observations and inflammatory cytokine productions of human macrophage phagocytising submicron titanium particles2010
Author(s)
Taira, M., Kagiya, T., Harada, H., Sasaki, M., Kimura, S., Narushima T., Nezu, T. and Araki, Y.
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Journal Title
J. Mater. Sci : Mater. Med.
Volume: 21
Pages: 267-275
Related Report
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[Journal Article] Amino acid residues modulating the activities of staphylococcal glutamyl endopeptidases2010
Author(s)
Ono, T., Ohara-Nemoto, Y., Shimoyama, Y., Okawara, H., Kobayakawa, T., Baba, T. T., Kimura, S. and Nemoto, T. K.
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Journal Title
Biol. Chem.
Volume: 391
Pages: 1221-1232
NAID
Related Report
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[Journal Article] Relationship between oral status and prevalence of periodontopathic bacteria on the tongues of elderly individuals2010
Author(s)
Kishi, M., Ohara-Nemoto, Y., Takahashi, M., Kishi, K., Kimura, S. and Yonemitsu, M.
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Journal Title
J. Med. Microbiol.
Volume: 59
Pages: 1354-1359
NAID
Related Report
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[Journal Article] Histological and TEM observation of subcutaneous tissues exposed to particulate copper, nickel and titanium2010
Author(s)
Saitoh, S., Sasaki, K., Nezu, T., Taira, M., Shimoyama, Y., Sasaki, M., Kimura, S. and Ishizeki, K.
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Journal Title
J. Oral Tissue Engin.
Volume: 8
Pages: 102-106
NAID
Related Report
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[Journal Article] Microscopic observations and inflammatory cytokine productions of human macrophage phagocytising submicron titanium particles.2010
Author(s)
Taira, M., Kagiya, T., Harada, H., Sasaki, M, Kimura, S., Narushima, T., Nezu, T., Araki, Y.
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Journal Title
J.Mater.Sci.Mater.Med.
Volume: 21
Pages: 267-275
Related Report
Peer Reviewed
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[Journal Article] Amino acid residues modulating the activities of staphylococcal glutamyl endopeptidases.2010
Author(s)
Ono, T., Ohara-Nemoto, Y., Shimoyama, Y. Okawara, H., Kobayakawa, T., Baba, T.T., Kimura, S., Nemoto, T.K.
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Journal Title
Biol.Chem.
Volume: 391
Pages: 1221-1232
NAID
Related Report
Peer Reviewed
-
[Journal Article] Relationship between oral status and prevalence of periodontopathic bacteria on the tongues of elderly individuals.2010
Author(s)
Kishi, M., Ohara-Nemoto, Y., Takahashi, M., Kishi, K, Kimura, S., Yonemitsu, M.
-
Journal Title
J.Med.Microbiol.
Volume: 59
Pages: 1354-1359
NAID
Related Report
Peer Reviewed
-
[Journal Article] Histological and TEM observation of subcutaneous tissues exposed to particulate copper, nickel and titanium.2010
Author(s)
Saitoh, S., Sasaki, K., Nezu, T., Taira, M., Shimoyama, Y., Sasaki, M., Kimura, S., Ishizeki, K.
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Journal Title
J.Oral Tissue Engin.
Volume: 8
Pages: 102-106
NAID
Related Report
Peer Reviewed
-
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[Journal Article] Relationship of quantitative salivary levels of Streptococcus mutans and S. sobrinus in mothers to caries status and colonization of mutans streptococci in plaque in their 2.5-year-old children2009
Author(s)
Kishi, M., Abe, A., Kishi, K., Ohara-Nemoto, Y., Kimura, S. and Yonemitsu, M.
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Journal Title
Community Dent. Oral Epidemiol.
Volume: 37
Pages: 241-249
Related Report
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[Journal Article] Gene expression analyses of human macrophage phagocytizing sub-μtitanium particles by allergy DNA chip(Genopal^<TM>)2009
Author(s)
Taira, M., Nezu, T., Sasaki, M., Kimura, S., Kagiya, T., Harada, H., Narushima T. and Araki, Y.
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Journal Title
Biomed. Mater. Eng
Volume: 19
Pages: 63-70
Related Report
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[Journal Article] DNA microarray analyses of the effects of LPS-stimulation and IC50 nickel ions on gene expressions of mouse macrophage-like cell line RAW2642009
Author(s)
Taira, M., Sasaki, M., Sasaki, K., Saitoh, S., Nezu, T., Kimura, S. and Araki, Y.
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Journal Title
Nano Biomed.
Volume: 1
Pages: 59-69
NAID
Related Report
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[Journal Article] First-stage genome-wide gene expression analyses of human mesenchymal stem cells exposed to IC50 Ni(2+) ions.2009
Author(s)
Taira, M., Kagiya, T., Sasaki, M., Sasaki, K., Saitoh, S., Nezu, T., Harada, H., Kimura, S. and Araki, Y.
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Journal Title
J. Oral Tissue Engin.
Volume: 7
Pages: 107-120
NAID
Related Report
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[Journal Article] Relationship of quantitative salivary levels of S.mutans and S.sobrinus in mothers to caries status and colonization of mutans streptococci in plaque in their 2.5-year-old children.2009
Author(s)
Kishi, M., Abe, A., Kishi, K., Ohara-Nemoto, Y., Kimura, S., Yonemitsu, M.
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Journal Title
Community Dent. Oral Epidemiol. 37
Pages: 241-249
Related Report
Peer Reviewed
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[Journal Article] Microscopic observations and inflammatory cytokine productions of human macrophage phagocytising submicron titanium particles.2009
Author(s)
Taira, M., Kagiya, T., Harada, H., Sasaki, M., Kimura, S., Narushima T., Nezu, T., Araki, Y.
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Journal Title
J.Mater.Sci : Mater. Med. 21
Pages: 267-275
Related Report
Peer Reviewed
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[Presentation] The pathogenic involvement of the fibronectin binding protein, Fbp62, in Streptococcus anginosus infection2011
Author(s)
Sasaki, M., Kodama, Y., Shimoyama, Y., Agato, S., Kimura, S.
Organizer
International Union of Microbiological Societies 2011 Congress (IUMS 2011)
Place of Presentation
Sapporo, Japan
Year and Date
2011-09-07
Related Report
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[Presentation] Auto-catalytic propeptide processing facilitates the final hetero-catalytic maturation of glutamyl endopeptidase GluV82011
Author(s)
Ohara-Nemoto, Y., Rouf, S. M. A., Shimoyama, Y., Kimura, S., Ono, T. and Nemoto, T. K.
Organizer
111th General Meeting of the American Society for Microbiology
Place of Presentation
New Orleans, USA
Related Report
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[Presentation] Fbp62, fibronectin binding protein of Streptococcus anginosus involves the virulence in mouse infection2011
Author(s)
Sasaki, M., Kodama, Y., Shimoyama, Y., Ishikawa, T. and Kimura, S.
Organizer
10th World Congress of Inflammation
Place of Presentation
Paris, France
Related Report
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[Presentation] The pathogenic involvement of the fibronectin binding protein, Fbp62, in Streptococcus anginosus infection.2011
Author(s)
Sasaki, M., Kodama, Y., Shimoyama, Y., Agato, S. and Kimura, S.
Organizer
International Union of Microbiological Societies 2011 Congress(IUMS 2011)
Place of Presentation
Sapporo, Japan
Related Report
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[Presentation] Prediction of colonization of periodontopathogens in plaque by VSC measurement.2010
Author(s)
Kishi, M., Ohara-Nemoto, Y., Kimura, S., Aizawa, F., Kishi, K., Takahashi, M., Yonemitsu, M.
Organizer
88th General Session & Exhibition of the IADR
Place of Presentation
Barcelona, Spain
Year and Date
2010-07-17
Related Report
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[Presentation] SLPI production from gingival epithelial cells against Porphyromonas gingivalis infection.2010
Author(s)
Ishikawa, T., Ohara-Nemoto, Y., Shimoyama, Y., Sasaki, N., Kimura, S.
Organizer
88th General Session & Exhibition of the IADR
Place of Presentation
Barcelona, Spain
Year and Date
2010-07-16
Related Report
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[Presentation] Structural chemistry of Staphylococcus aureus glutamyl endopeptidase.2010
Author(s)
Ohara-Nemoto, Y., Rouf, S.M.A., Shimoyama, Y., Kimura, S., Nemoto, T.K.
Organizer
88th General Session & Exhibition of the IADR
Place of Presentation
Barcelona, Spain
Year and Date
2010-07-15
Related Report
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[Presentation] Adhesion mechanism of Streptococcus anginosus to mucosal epithelial cells.2010
Author(s)
Kodama, Y., Sasaki, M., Shimoyama, Y., Tajika, S., Kimura, S.
Organizer
20th European Congress of Clinical Microbiology and Infectious Diseases (20th ECCMID 2010)
Place of Presentation
Vienna, Austria
Year and Date
2010-04-13
Related Report
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[Presentation] Prediction of colonization of periodontopathogens in plaque by VSC measurement2010
Author(s)
Kishi, M., Ohara-Nemoto, Y., Kimura, S., Aizawa, F., Kishi, K., Takahashi, M. and Yonemitsu, M.
Organizer
88th General Session & Exhibition of the IADR
Place of Presentation
Barcelona, Spain
Related Report
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[Presentation] Maturation processing of glutamyl endopeptidase of Staphylococcus epidermidis.2009
Author(s)
Ohara-Nemoto, Y., Ono, T.Shimoyama, Y., Kimura, S., Nemoto, T.K.
Organizer
109th General Meeting of the American Society for Microbiology
Place of Presentation
Philadelphia, PA, USA
Year and Date
2009-05-20
Related Report
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