| Project/Area Number |
21592347
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
|
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| Research Institution | Tsurumi University |
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Principal Investigator |
INOUE Hiroko 鶴見大学, 歯学部, 准教授 (50367306)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
SAITO Ichiro 鶴見大学, 歯学部, 教授 (60147634)
MISHIMA Kenji 昭和大学, 歯学部, 教授 (50275343)
RYO Kofuchi 鶴見大学, 歯学部, 講師 (10298268)
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| Project Period (FY) |
2009 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | EBウイルス / シェーグレン症候群 / TLR / 唾液腺 / BAFF |
|---|
| Research Abstract |
The salivary gland epithelial cells, HSY cells, were transfected with CD21-HA plasmid. EBV positive-B cell line, Akata, was cocultured with CD21-HSY cells for establishment of EBV positive salivary gland epithelial cells. The expression plasmids, TLR3, TLR8, and TLR9-HA, were prepared. To study of BAFF transcription, BAFF promoter region was flanked with luciferase coding region, BAFF-Luc. BALB/c mice were immunized with nuclear extract from Akata, and will be evaluated with ELISA for SSB/La, which was established with recombinant SSB/La-GST protein.
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