Standardization of stem cells for practical application

• Project/Area Number: 21592351
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Tokyo Ariake University of Medical and Health Sciences
• Principal Investigator: TERAI Masanori 東京有明医療大学, 保健医療学部, 教授 (70359917)
• Co-Investigator(Kenkyū-buntansha): UMEZAWA Akihiro 独立行政法人国立成育医療研究センター研究所, 再生医療センター生殖・細胞医療研究部, 部長 (70213486) ; 藤本 純一郎 国立成育医療センター, 生殖医療研究部, 副所長 (60175578) ; 秦 順一 国立成育医療センター, 名誉総長 (90051614)
• Project Period (FY): 2009-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
• Keywords: 再生医学 / 病理学 / 遺伝子 / ウイルス / 組織・細胞 / テロメア / iPS細胞 / 間葉系幹細胞 / 細胞・組織

Research Abstract

Human induced pluripotent stem cells (iPSCs) and mesenchymal stem cells have pluripotent ability to differentiate into bone, cartilage, fat, heart, nerve, liver and skeletal muscle. These stem cells derived from autologous cells have high potential value for stem cell-based therapies for incurable or lifestyle-related diseases. We have already reported that telomeres of the established iPSCs derived from human amnion and fetal lung fibroblasts (MRC-5) were significantly longer than those of the parental cells, and that the telomere extension rates varied quite significantly among the clones without chromosomal instability, although the telomeres of other iPSCs derived from MRC-5 became shorter as the number of passages increased, along with chromosomal abnormalities from an early stage. In the future, with a view to clinical application, there will be a need to analyze in detail problems such as chromosomal abnormalities in both mesenchymal stem cells and human iPSCs.

Research Products

• [Journal Article] Investigation of telomere length dynamics in induced pluripotent stem cells using quantitative fluorescence in situ hybridization (2013)
  • Author(s): Terai M, Izumiyama-Shimomura N, Aida J, Ishikawa N, Kuroiwa M, Poon SS, Arai T, Toyoda M, Akutsu H, Umezawa A, Takubo K
  • Journal Title: Tissue & Cell Volume: 45号6巻 Pages: 407-413
  • Peer Reviewed
• [Journal Article] Standardization of Stem Cells for Practical Application (2013)
  • Author(s): Terai M
  • Journal Title: Annals of Clinical Pathology Volume: 1号1巻 Pages: 1002-1002
  • Peer Reviewed
• [Journal Article] Association of telomere shortening in myocardium with heart weight gain and cause of death (2013)
  • Author(s): Terai M, Izumiyama-Shimomura N, Aida J, Ishikawa N, Sawabe M, Arai T, Fujiwara M, Ishii A, Nakamura K, Takubo K
  • Journal Title: Scientific reports Volume: 3号 Pages: 2401-2401
  • Peer Reviewed
• [Journal Article] Telomere shortening in the esophagus of Japanese alcoholics : relationships with chromoendoscopic findings, ALDH2 and ADH1B genotypes and smoking history (2013)
  • Author(s): Aida J, Yokoyama A, Shimomura N, Nakamura K, Ishikawa N, Terai M, Poon S, Matsuura M, Fujiwara M, Sawabe M, Arai T, Takubo K
  • Journal Title: PloS one Volume: 8号
  • Peer Reviewed
• [Journal Article] Investigation of telomere length dynamics in induced pluripotent stem cells using quantitative fluorescence in situ hybridization. (2013)
  • Author(s): Terai M, Izumiyama-Shimomura N, Aida J, Ishikawa N, Kuroiwa M, Poon SS, Arai T, Toyoda M, Akutsu H, Umezawa A, Takubo K.
  • Journal Title: Tissue & Cell Volume: 45(6) Issue: 6 Pages: 407-413
  • DOI: 10.1016/j.tice.2013.07.003
  • Peer Reviewed
• [Journal Article] Standardization of Stem Cells for Practical Application (2013)
  • Author(s): TERAI Masanori
  • Journal Title: Annals of Clinical Pathology Volume: 1(1) Pages: 1002-1002
  • Peer Reviewed
• [Journal Article] Association of telomere shortening in myocardium with heart weight gain and cause of death. (2013)
  • Author(s): Terai M, Izumiyama-Shimomura N, Aida J, Ishikawa N, Sawabe M, Arai T, Fujiwara M, Ishii A, Nakamura K, Takubo K
  • Journal Title: Scientific reports Volume: 3 Issue: 1 Pages: 2401-2401
  • DOI: 10.1038/srep02401
  • Peer Reviewed
• [Journal Article] Telomere shortening in the esophagus of Japanese alcoholics: relationships with chromoendoscopic findings, ALDH2 and ADH1B genotypes and smoking history. (2013)
  • Author(s): Aida J, Yokoyama A, Shimomura N, Nakamura K, Ishikawa N, Terai M, Poon S, Matsuura M, Fujiwara M, Sawabe M, Arai T, Takubo K
  • Journal Title: PloS one Volume: 8 Issue: 5 Pages: 1-7
  • DOI: 10.1371/journal.pone.0063860
  • Peer Reviewed
• [Journal Article] Dystrophin conferral using human endothelium expressing HLA-E in the non-immunosuppressive murine model of Duchenne muscular dystrophy (2011)
  • Author(s): Cui CH, Miyoshi S, Tsuji H, Makino H, Kanzaki S, Kami D, Terai M, Suzuki H, Umezawa A
  • Journal Title: Human molecular genetics Volume: 20巻 Pages: 235-244
  • Peer Reviewed
• [Journal Article] Dystrophin conferral using human endothelium expressing HLA-E in the non-inmuno suppressive murine model of Ducheme muscular dystrophy (2011)
  • Author(s): Cui CH, Terai M, Umezawa A
  • Journal Title: Human Molecular Genetics Volume: 20 Pages: 235-244
  • Peer Reviewed
• [Journal Article] Dystrophin conferral using human endothelium expressing HLA-E in the non-immuno suppressive murine model of Duchenne muscular dystrophy. (2011)
  • Author(s): Cui CH, Terai M.Omezawa A
  • Journal Title: Human Molecular Genetics Volume: 20 Pages: 235-244
  • Peer Reviewed
• [Journal Article] 再生医療の将来と産婦人科 5.間葉系幹細胞の老化 (2009)
  • Author(s): 寺井政憲, 梅澤明弘
  • Journal Title: 産科と婦人科 Volume: 76号10巻 Pages: 1196-1202
• [Journal Article] CCN3 and bone marrow cells (2009)
  • Author(s): Katsube K, Ichikawa S, Katsuki Y, Kihara T, Terai M, Lau LF, Tamamura Y, Takeda S, Umezawa A, Sakamoto K, Yamaguchi A
  • Journal Title: Journal of cell communication and signaling Volume: 3巻 Pages: 135-145
  • Peer Reviewed
• [Journal Article] 間葉系幹細胞の老化 (2009)
  • Author(s): 寺井政憲、梅澤明弘
  • Journal Title: 産科と婦人科 76-10 Pages: 1196-1202
• [Journal Article] CCN3 and bone marrow cells. (2009)
  • Author(s): Katsube K, Ichikawa S, Katsuki Y, Kihara T, Terai M, Lau LF, Tamamura Y, Takeda S, Umezawa A, Sakamoto K, Yamaguchi A.
  • Journal Title: Journal of Cell Communication and Signaling 3-2 Pages: 135-145
  • Peer Reviewed
• [Presentation] 心疾患による心重量増加と心筋のテロメア短縮の関連について (2014)
  • Author(s): 寺井政憲、仲村賢一、泉山-下村七生貴、石川直、相田順子、田久保海誉
  • Organizer: 第13回日本再生医療学会総会
  • Place of Presentation: 京都
  • Year and Date: 2014-03-05
• [Presentation] iPS細胞のテロメアは長くなるか、短くなるか? (2014)
  • Author(s): 寺井政憲、仲村賢一、泉山-下村七生貴、石川直、豊田雅士、相田順子、田久保海誉
  • Organizer: 第13回日本再生医療学会総会
  • Place of Presentation: 京都
  • Year and Date: 2014-03-04
• [Presentation] iPS細胞のテロメアは長くなるか、短くなるか? (2013)
  • Author(s): 寺井政憲、仲村賢一、泉山-下村七生貴、石川直、豊田雅士、相田順子、田久保海誉
  • Organizer: 第25回高遠分子細胞生物学シンポジウム
  • Place of Presentation: 比叡山延暦寺会館
  • Year and Date: 2013-08-29
• [Presentation] iPS細胞化によるリプログラミング過程でのテロメア動態のQ-FISH法による解析 (2013)
  • Author(s): 石川直、仲村賢一、泉山-下村七生貴、相田順子、寺井政憲、豊田雅士、田久保海誉
  • Organizer: 第102回日本病理学会総会
  • Place of Presentation: 札幌
  • Year and Date: 2013-06-07
• [Presentation] Telomeres of human induced pluripotent stem cells are elongated during reprogramming, but show inter-clonal differences in extension rate (2013)
  • Author(s): Ishikawa N, Nakamura K, Shimomura-Izumiyama N, Terai M, Toyoda M and Takubo K
  • Organizer: CiRA International Symposium 2013
  • Place of Presentation: 京都
  • Year and Date: 2013-03-11
• [Presentation] Chromosomal instability could be induced by telomere shortening although established iPSCs maintain telomerase activity (2013)
  • Author(s): Terai M, Ishikawa N, Toyoda M, Aida J and Takubo K
  • Organizer: CiRA International Symposium 2013
  • Place of Presentation: 京都
  • Year and Date: 2013-03-11
• [Presentation] 間葉系幹細胞は再生医療の場で主役となりうる貴重な細胞ソースである (2009)
  • Author(s): 寺井政憲
  • Organizer: 第30回日本アフェレシス学会
  • Place of Presentation: 札幌
  • Year and Date: 2009-09-11
  • Invited
• [Presentation] 間葉系幹細胞は再生医療の場で主役となりうる貴重な細胞ソースである (2009)
  • Author(s): 寺井政憲
  • Organizer: 第30回日本アフェレシス学会
  • Place of Presentation: 京王プラザホテル札幌北海道札幌市
  • Year and Date: 2009-09-11
• [Presentation] 心疾患による心重量増加と心筋のテロメア短縮の関連について
  • Author(s): 寺井政憲、仲村賢一、泉山-下村七生貴、石川直、相田順子、 田久保海誉
  • Organizer: 第13回日本再生医療学会総会
  • Place of Presentation: 京都
• [Presentation] iPS細胞のテロメアは長くなるか、短くなるか？
  • Author(s): 寺井政憲、仲村賢一、泉山-下村七生貴、石川直、豊田雅士、 相田順子、田久保海誉
  • Organizer: 第13回日本再生医療学会総会
  • Place of Presentation: 京都
• [Presentation] iPS細胞のテロメアは長くなるか、短くなるか？
  • Author(s): 寺井政憲、仲村賢一、泉山-下村七生貴、石川直、豊田雅士、 相田順子、田久保海誉
  • Organizer: 第25回高遠分子細胞生物学シンポジウム
  • Place of Presentation: 比叡山延暦寺会館
• [Presentation] Chromosomal instability could be induced by telomere shortening although established iPSCs maintain telomerase activity.
  • Author(s): Masanori Terai, Naoshi Ishikawa, Masashi Toyoda, Ken-ichi Nakamura, and Kaiyo Takubo
  • Organizer: CiRA International Symposium 2013
  • Place of Presentation: 京都
• [Presentation] Telomeres of human induced pluripotent stem cells are elongated during reprogramming, but show inter-clonal differences in extension rate
  • Author(s): Naoshi Ishikawa, Ken-ichi Nakamura, Naotaka Shimomura-Izumiyama, Masanori Terai, Masashi Toyoda, and Kaiyo Takubo
  • Organizer: CiRA International Symposium 2013
  • Place of Presentation: 京都