Involvement of a novel signaling molecule, PRIP in the regulation of bone metabolism through reproduction system

• Project/Area Number: 21592365
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional basic dentistry
• Research Institution: Kyushu University
• Principal Investigator: MATSUDA Miho 九州大学, 歯学研究院, 助教 (40291520)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 生殖・ホルモン・骨代謝 / 骨代謝 / 骨密度 / PRIP / 破骨細胞 / 骨芽細胞 / ホルモン

Research Abstract

This study revealed the following :(i) PRIP was involved in female reproductive system, especially in gonadotropins secretion and ovarian follicle maturation.(ii) The bone mineral density and trabecular bone volume were higher in PRIP-gene deficient mice due to enhanced bone formation and decreased bone resorption, indicating that PRIP is implicated in the negative regulation of bone formation.

Research Products

• [Journal Article] Involvement of PRIP, phospholipase C-related, but catalytically inactive protein (2011)
  • Author(s): Tsutsumi, K., Matsuda, M., Kotani, M., Murakami, A., Mizokami, A., Takahashi, I., Terada, Y., Kanematsu, T., Fukami, K., Takenawa, T., Jimi, E. and Hirata, M.
  • Journal Title: in bone formation. J. Biol. Chem Volume: 286 Pages: 31032-31042
  • Peer Reviewed
• [Journal Article] Involvement of PRIP, Phospholipase C-related, but Catalytically Inactive Protein, in Bone Formation (2011)
  • Author(s): Koshiro Tsutsumi, Miho Matsuda, Eijiro Jimi, Masato Hirata, 他
  • Journal Title: Journal of Biological Chemistry Volume: 286 Pages: 31032-31042
  • Peer Reviewed
• [Journal Article] Dual signaling pathways involved in arterial tone regulation by extracellular UTP in the rat (2011)
  • Author(s): Megumi Sugihara, Hiromitsu Morita, Miho Matsuda, Masato Hirata, 他
  • Journal Title: Journal of Pharmacological Science Volume: 115 Pages: 293-308
  • Peer Reviewed
• [Journal Article] Obstruction enhances rho-kinase pathway and diminishes protein kinse C pathway in carbachol-induced calcium sensitization in α-toxin permeabilized Guinea pig detrusor smooth muscle (2011)
  • Author(s): Nouval Shahab, Shunichi Kajioka,, Miho Matsuda, Seiji Naito, 他
  • Journal Title: Neurourology and Urodynamics Volume: 31 Pages: 593-599
  • Peer Reviewed
• [Journal Article] Assay of dense-core vesicle exocytosis using permeabilized PC12 cells (2010)
  • Author(s): Gao, J., Takeuchi, H., Umebayashi, H., Zhang, Z., Matsuda, M. and Hirata, M.
  • Journal Title: Adv. Enz. Regul Volume: 50 Pages: 237-246
  • Peer Reviewed
• [Journal Article] Assay of dense-core vesicle exocytosis using permeabilized PC12 cells (2010)
  • Author(s): Jing Gao, Hiroshi Takeuchi, Hisanori Umebayashi, Zhao Zhang, M Matsuda, Masato Hirata
  • Journal Title: Advances in Enzyme Regulation Volume: 50 Pages: 237-246
  • Peer Reviewed
• [Journal Article] Involvement of phospholipase C-related inactive protein in the mouse reproductive system through the regulation of gonadotropin levels (2009)
  • Author(s): Matsuda, M, Tsutsumi, K., Kanematsu, T., Fukami, K., Terada, Y., Takenawa, T., Nakayama, K. I., Hirata, M.
  • Journal Title: Biol Reprod Volume: 81 Pages: 681-689
  • Peer Reviewed
• [Journal Article] Involvement of Phospholipase C-related inactive protein in the Mouse Reproductive System through the Regulation of Gonadotropin Levels (2009)
  • Author(s): M Matsuda, 他7名
  • Journal Title: Biology of Reproduction 81 Pages: 681-689
  • Peer Reviewed
• [Presentation] 新規情報伝達分子PRIPノックアウトマウスにおける生殖制御の異常 (2011)
  • Author(s): 松田美穂、平田雅人
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-13
• [Presentation] 新規情報伝達分子PRIPノックアウトマウスにおける生殖制御の異常 (2011)
  • Author(s): Miho Matsuda, Masato Hirata
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜)
  • Year and Date: 2011-12-13
• [Presentation] PRIP is implicated in the negative regulation of bone formation (2011)
  • Author(s): 小谷美穂、堤康史郎、松田美穂、平田雅人
  • Organizer: International Symposium, New Aspects of Phospholipid Biology and Medicine 2011
  • Place of Presentation: 福岡
  • Year and Date: 2011-11-15
• [Presentation] PRIP is implicated in the negative regulation of bone formation (2011)
  • Author(s): Miho Kotani, Koshiro Tsutsumi, Miho Matsuda, Masato Hirata
  • Organizer: International Symposium, New Aspects of Phospholipid Biology and Medicine 2011
  • Place of Presentation: ホテルルイガンズ会議場(福岡)
  • Year and Date: 2011-11-15
• [Presentation] PRIPはautophagyを抑制的に制御する (2011)
  • Author(s): 梅林久範、松田美穂、兼松隆
  • Organizer: 第53回歯科基礎医学会学術大会
  • Place of Presentation: 岐阜
  • Year and Date: 2011-09-30
• [Presentation] PRIP遺伝子欠損マウスで観察された骨の表現型の解析 (2011)
  • Author(s): 堤康史郎、松田美穂、小谷美穂、平田雅人(他)
  • Organizer: 第53回歯科基礎医学会学術大会
  • Place of Presentation: 岐阜
  • Year and Date: 2011-09-30
• [Presentation] PRIP遺伝子欠損マウスで観察された骨の表現型の解析 (2011)
  • Author(s): 堤康史郎、松田美穂、小谷美穂、溝上顕子、自見英治郎、平田雅人
  • Organizer: 第53回歯科基礎医学会学術大会
  • Place of Presentation: 長良川国際会議場(岐阜)
  • Year and Date: 2011-09-30
• [Presentation] PRIPはautophagyを抑制的に制御する (2011)
  • Author(s): 梅林久範、松田美穂、溝上顕子、竹内弘、平田雅人、兼松隆
  • Organizer: 第53回歯科基礎医学会学術大会
  • Place of Presentation: 長良川国際会議場(岐阜)
  • Year and Date: 2011-09-30
• [Presentation] 骨形成制御機構におけるPRIPの機能解析 (2011)
  • Author(s): 小谷美穂、堤康史郎、松田美穂、平田雅人
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 国立京都国際会館(京都)
  • Year and Date: 2011-09-22
• [Presentation] Involvement of PRIP in the regulation of bone metabolism (2010)
  • Author(s): 」松田美穂、平田雅人
  • Organizer: 第83回日本生化学会・第33回日本分子生物学会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-09
• [Presentation] 新規情報伝達分子PRIPの性周期制御における機能 (2010)
  • Author(s): 松田美穂、平田雅人
  • Organizer: 第83回日本生化学会・第33回日本分子生物学会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-09
• [Presentation] The role of PRIP in the regulation of autophagy (2010)
  • Author(s): 梅林久範、松田美穂、兼松隆、平田雅人
  • Organizer: 第12回IUBMB & 第21回FAOBMB(国際生化学分子生物学連合カンファレンス)
  • Place of Presentation: メルボルン
  • Year and Date: 2010-09-30
• [Presentation] 「Regulatory role of PRIP in the bone formation (2010)
  • Author(s): 堤康史郎、松田美穂、自見英治郎、平田雅人
  • Organizer: 第12回IUBMB & 第21回FAOBMB(国際生化学分子生物学連合カンファレンス)
  • Place of Presentation: メルボルン
  • Year and Date: 2010-09-30
• [Presentation] Pituitary and ovarian function in PRIP knockout mice (2010)
  • Author(s): 松田美穂、平田雅人
  • Organizer: 第12回IUBMB & 第21回FAOBMB(国際生化学分子生物学連合カンファレンス)
  • Place of Presentation: メルボルン
  • Year and Date: 2010-09-29
• [Presentation] Regulatory role of PRIP in the bone formation (2010)
  • Author(s): Koshiro Tsutsumi, Miho Matsuda, Eijiro Jimi, Masato Hirata
  • Organizer: 第12回IUBMBならびに第21回EAOBMB(国際生化学分子生物学連合カンファレンス)
  • Place of Presentation: Melbourne (Australia)
  • Year and Date: 2010-09-29
• [Presentation] Pituitary and ovarian function in PRIP knockout mice (2010)
  • Author(s): Miho Matsuda, Masato Hirata
  • Organizer: 第12回IUBMBならびに第21回FAOBMB(国際生化学分子生物学連合カンファレンス)
  • Place of Presentation: Melbourne (Australia)
  • Year and Date: 2010-09-28
• [Presentation] PRIPは骨形成の制御に関与する (2010)
  • Author(s): 」堤康史郎、松田美穂、平田雅人
  • Organizer: PRIPは骨形成の制御に関与第52回歯科基礎医学会学術大会・総会
  • Place of Presentation: 東京
  • Year and Date: 2010-09-21
• [Presentation] Involvement of PRIP in the regulation of bone metabolism (2009)
  • Author(s): 松田美穂
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-10
• [Presentation] 骨代謝制御における新規情報伝達分子PRIPの関与 (2009)
  • Author(s): 松田美穂, 堤康史郎, 久木田敏夫, 平田雅人
  • Organizer: 分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-10
• [Presentation] Involvement of PRIP in the reproduction system through the regulation of gonadotropin levels (2009)
  • Author(s): 」松田美穂、平田雅人
  • Organizer: 第6回Japan-Korea Conference on Cellular Signaling for Young Scientists
  • Place of Presentation: 佐世保
  • Year and Date: 2009-11-24
• [Presentation] Involvement of PRIP in the reproductive system through the regulation of gonadotropin levels (2009)
  • Author(s): M Matsuda, Koushirou Tsutsumi, Takashi Kanematsu, Masato Hirata
  • Organizer: The 6^<th> Japan-Korea conference on cellular signaling for young scientists(細胞シグナリングに関する第6回日韓若手交流セミナー)
  • Place of Presentation: 佐世保
  • Year and Date: 2009-11-24
• [Presentation] Involvement of PRIP in the regulation of bone metabolism (2009)
  • Author(s): 堤康史郎、松田美穂、平田雅人
  • Organizer: 第32回日本生化学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-10
• [Presentation] 「PRIPの骨代謝における機能解析 (2009)
  • Author(s): 堤康史郎、松田美穂、平田雅人
  • Organizer: 第51回歯科基礎医学会学術大会・総会
  • Place of Presentation: 最優秀ポスター賞受賞
  • Year and Date: 2009-09-10
• [Presentation] Involvement of a novel inositol 1, 4, 5-trisphosphate binding protein, PRIP, in the reproductive system through the regulation of gonadotropin levels (2009)
  • Author(s): 松田美穂、平田雅人
  • Organizer: 第21回IUBMB & 第12回FAOBMB(国際生化学分子生物学連合カンファレンス)
  • Place of Presentation: 上海
  • Year and Date: 2009-08-06
• [Presentation] Involvement of a novel inositol 1,4,5-trisphosphate binding protein, PRIP, in the reproductive system through the regulation of gonadotropin levels (2009)
  • Author(s): M Matsuda, Koushirou Tsutsumi, Takashi Kanematsu, Masato Hirata
  • Organizer: 第21回IUBMBならびに第12回FAOBMB(国際生化学分子生物学連合カンファレンス)
  • Place of Presentation: 上海(中国)
  • Year and Date: 2009-08-05
• [Presentation] 骨形成制御機構におけるPRIPの機能解析
  • Author(s): 小谷美穂、堤康史郎、松田美穂、平田雅人
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都