The investication of factors related to odontoblast diffenetiation and morphology preservation, and analysis of expression

• Project/Area Number: 21592491
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Tsurumi University
• Principal Investigator: FUJIHARA Hisako 鶴見大学, 歯学部, 助教 (80396746)
• Co-Investigator(Kenkyū-buntansha): HOSHI Kazuto 東京大学, 医学部・附属病院, 特任准教授 (30344451) ; IINO Mitsuyoshi 山形大学, 医学部・附属病院, 教授 (50212717) ; CHIKAZU Daichi 東京医科大学, 医学部・附属病院, 教授 (30343122) ; FUJIHARA Yuko 東京大学, 医学(系)研究科(研究院), 特任助教 (50466744)
• Co-Investigator(Renkei-kenkyūsha): TAKATO Tsuyoshi 東京大学, 医学部・附属病院, 教授 (90171454) ; MASUTANI Mitsuko 国立がん研究センター研究所, ゲノム安定性研究分野, 分野長 (60238904)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 再生歯学 / ポリADP-リボシル化反応 / 再生医療 / ノックアウトマウス / ポリADP-リボシル化 / 象牙芽細胞 / 歯髄 / ポリADP-リボース合成酵素

Research Abstract

In this report, Parp-1 was suggested to involve in odontoblast/osteoblast differentiation from mesenchymal stem cell(MSC) and factors related to morphology preservation. Through the time course analysis of mice teeth, dysplasia and malformation inside the teeth were found based on the two factors. Parp-1 deficiency and aging. In vitro differentiation inducement from MSCs, odontoblast and osteoblast differentiation was not observed under the inhibition of Parp activity.

Research Products

• [Journal Article] Cyclooxygenase-2 activity is important in craniofacial fracture repair (2010)
  • Author(s): Chikazu D, Fujikawa Y, Fujihara H, Suenaga H, Saijo H, Ohkubo K, Ogasawara T, Mori Y, Iino M, Takato T
  • Journal Title: Int J Oral Maxillofac Surg Volume: 40(3) Pages: 322-326
• [Journal Article] Improvement in wound healing by a novel synthetic collagen-gel dressing in genetically diabetic mice (2010)
  • Author(s): Chikazu D, Taguchi T, Koyama H, Hikiji H, Fujihara H, Suenaga H, Saijo H, Mori Y, Seto I, Iino M, Takato T
  • Journal Title: Asian J Oral Maxillofac Surg Volume: 22(2) Pages: 61-67
• [Journal Article] Metastasis of hepatocellular carcinoma in to the mandible with radiographic findings mimicking a radicular cyst : a case report (2010)
  • Author(s): Fujihara H, Chikazu D, Saijo H, Suenaga H, Mori Y, Iino M, Hamada Y, Takato T
  • Journal Title: J Endodontics Volume: 36(9) Pages: 1593-1596
• [Journal Article] Evaluation and analysis of formation of bone at the palate in patients with cleft lip and palate after palatoplasty based on computed tomograms and three-dimensional data (2010)
  • Author(s): Saijo H, Mori Y, Fujihara H, Kanno Y, Chikazu D, Ohkubo K, Hikiji H, Iino M, Yonehara Y, Takato T
  • Journal Title: Scand J Plast Surg Hand Surg Volume: 44 Pages: 21-25
• [Journal Article] Evaluation and analysis of formation of bone at the palate in patients with cleft lip and palate after palatoplasty based on computed tomograms and three-dimensional data. (2010)
  • Author(s): Saiio H, Mori Y, Fujihara H, et.al.
  • Journal Title: J Plast Surg Hand Surg. Volume: 44 Pages: 21-25
  • Peer Reviewed
• [Journal Article] Metastasis of hepatocellular carcinoma in to the mandible with radiographic findings mimicking a radicular cyst : a case report. (2010)
  • Author(s): Fujihara H, Chikazu D, et.al.
  • Journal Title: J Endodontics Volume: 36(9) Pages: 1593-1596
  • Peer Reviewed
• [Journal Article] Improvement in wound healing by a novel synthetic collagen-gel dressing in geneticaly diabetic mice. (2010)
  • Author(s): Chikazu D, Taguchi T, Koyama H, Hikiji H, Fujihara H, et.al.
  • Journal Title: Asian J Oral Maxillofac Surg Volume: 22(2) Pages: 61-67
  • Peer Reviewed
• [Journal Article] Dermoid cyst with fistula on the dorsum of the dorsum of the tongue (2009)
  • Author(s): Mori Y, Saijo H, Fujihara H, Tanaka Y, Maeda Y, Hayashi N, Chikazu D, Iino M, Takato T
  • Journal Title: Asian J Oral Maxillofac Surg Volume: 21 Pages: 51-53
• [Journal Article] Poly(ADP-ribose) glycohydrolase deficiency sensitizes mouse ES cells to particular types of DNA damaging agents (2009)
  • Author(s): Fujihara H, Ogino H, Maeda D, Shirai H, Nozaki T, Kamada N, Jishage K, Tanuma S, Takato T, Nakagama H, Sugimura T, Masutani M
  • Journal Title: Curr Cancer Drug Target Volume: 9(8) Pages: 953-962
• [Journal Article] Poly(ADP-ribose) glycohydrolase deficiency sensitizez mouse ES cells particular types of DNA damaging agents. (2009)
  • Author(s): Fujihara H., Ogino H., et.al.
  • Journal Title: Current Cancer Drug Target 9(8) Pages: 953-962
  • Peer Reviewed
• [Journal Article] 口外法により下顎枝舌側部に再発したエナメル上皮腫を切除した1例。 (2009)
  • Author(s): 近津大地、藤原久子, 他4名
  • Journal Title: 日本口腔外科学会誌 55(5) Pages: 241-245
  • NAID: 10027090393
  • Peer Reviewed
• [Journal Article] Solitary fibrous tumor of the buccal space resected in combination with coronoidectomy
  • Author(s): Yamada H, Hamada Y, Fujihara H, Fukami K, Mishima K, Nakaoka K, Kumagai K, Imamura E
  • Journal Title: Oral Surg Oral Med Oral Pathol Oral Radiol Endod Volume: (in press)
• [Journal Article] Solitary fibrous tumor of the buccal space resected in combination with coronoidectomy
  • Author(s): Yamada H, Hamada Y, Fujihara H, et.al
  • Journal Title: Oral Surg Oral Med Oral Pathol Oral Radiol Endod Volume: (In press)
  • Peer Reviewed
• [Journal Article] Dermoid cyst with fistula formation on the dorsum of the tongue -Acase report.
  • Author(s): Mori Y., Saijo H., Fujihara H., et.al.
  • Journal Title: Asian Journal of Oral and Maxillofacial Surgery (In press)
  • Peer Reviewed
• [Presentation] 口腔ケア委員会の活動 (2012)
  • Author(s): 藤原久子
  • Organizer: 第11回相模原協同病院院内学術集会
  • Place of Presentation: 神奈川県相模原市相模原協同病院
  • Year and Date: 2012-03-10
• [Presentation] 咬筋内に生じた脂肪腫の1例 (2011)
  • Author(s): 圓谷郷, 山田浩之, 藤原久子, 堀内俊克, 橘竜佑, 里村一人, 濱田良樹
  • Organizer: 第191回日本口腔外科学会関東地方会
  • Place of Presentation: 横浜
  • Year and Date: 2011-07-11
• [Presentation] 相模原協同病院病棟看護師における口腔ケアに対する意識調査 (2011)
  • Author(s): 藤原久子, 中島敏文, 大久保多佳子, 小俣舞子, 濱田良樹
  • Organizer: 第8回日本口腔ケア学会・学術総会
  • Place of Presentation: 東京
• [Presentation] 下顎歯肉に発症した筋線維腫の一例 (2010)
  • Author(s): 田中庸子, 山田浩之, 斎藤知之, 藤原久子, 川口浩司, 濱田良樹
  • Organizer: 第189回日本口腔外科学会関東地方会
  • Place of Presentation: 栃木
  • Year and Date: 2010-06-19
• [Presentation] Involvement of poly(ADP-ribose) polymerase-1(Parp-1) in odontoblast differentiation in aging process (2010)
  • Author(s): Fujihara H., Tsutsumi M., Kusuoka O., Hamada Y., Sugimura T., Masutani M
  • Organizer: 18th International Conference on ADP-ribose metabolism
  • Place of Presentation: Zurich
• [Presentation] Involvement of poly(ADP-ribose) polymerase-1(Parp-1) in odontogenic function (2010)
  • Author(s): Fujihara H., Hamada Y., Tsutsumi M., Kusuoka O., Sugimura T., Masutani M
  • Organizer: 88th IADR General Session
  • Place of Presentation: Barcelona
• [Presentation] 口腔悪性腫瘍との鑑別を要したパラコクシジオイデス症の1例 (2010)
  • Author(s): 藤原久子, 山田浩之, 寺田知加, 神谷洋子, 田中庸子, 浅田洸一, 里村一人, 濱田良樹
  • Organizer: 第20回日本口腔粘膜学会総会・学術集
  • Place of Presentation: 大阪
• [Presentation] Involvement of poly(ADP-ribose) polymerase-1 (Parp-1) in odontogenic function. (2010)
  • Author(s): Fuiihara H., Hamada Y., et al.
  • Organizer: 88^<th> IADR General Session
  • Place of Presentation: Barcelona
• [Presentation] Involvement of poly(ADP-ribose) polymerase-1 (Parp-1) in odontoblast differentiation in aging process. (2010)
  • Author(s): Fujihara H., Masutani, et al.
  • Organizer: 18^<th> International Conference on ADP-ribose metabolism
  • Place of Presentation: Zurich
• [Presentation] 腫瘍内の出血が基点と考えられた嚢胞形成多形性腺腫の1例 (2009)
  • Author(s): 中野永美子, 藤原久子, 堀江彰久, 中谷安典, 山崎陽子, 田中庸子, 新井剛, 山田浩之, 川口浩司, 濱田良樹
  • Organizer: 鶴見大学歯学会70回例会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-19
• [Presentation] 大きな嚢胞を形成し診断に苦慮した多形性腺腫の1例 (2009)
  • Author(s): 中野永美子, 藤原久子, 堀江彰久, 中谷安典, 山崎陽子, 田中庸子, 新井剛, 山田浩之, 川口浩司, 濱田良樹
  • Organizer: 第43回日本口腔科学会関東地方部会
  • Place of Presentation: 東京
  • Year and Date: 2009-10-03
• [Presentation] 大きな襄胞を形成し診断に苦慮した多形性腺腫の1例 (2009)
  • Author(s): 中野永美子、藤原久子, 他
  • Organizer: 第43回 日本口腔科学会関東地方部会
  • Place of Presentation: 東京
  • Year and Date: 2009-10-03