Developments of scaffolds and processing technology to maximize the osteogenic potential of cultured periosteal sheets

• Project/Area Number: 21592492
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Niigata University
• Principal Investigator: KAWASE Tomoyuki 新潟大学, 医歯学系, 准教授 (90191999)
• Co-Investigator(Kenkyū-buntansha): OKUDA Kazuhiro 新潟大学, 医歯学系, 准教授 (00169228)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 培養骨膜シート / 骨再生 / 細胞基材 / 細胞プロセッシング

Research Abstract

We aimed to develop the fast processing methodology and novel scaffolds for human periosteal sheets. In general, scaffolds made of synthetic biodegradable polymers are not suitable for cell adhesion. However, our porous PLLA scaffold was capable of well securing periosteal sheets. This scaffold also functioned to facilitate extracellular matrices and cell penetration into deep pore regions. In this project, we have successfully developed several promising scaffolds with biodegradable polymermaterials.

Research Products

• [Journal Article] Bioactivity of freeze-dried platelet-rich plasma in an adsorbed form on a biodegradable polymer material (2012)
  • Author(s): Nakajima Y, Kawase T, Okuda K, Wolff LF, Yoshie H
  • Journal Title: Platelets Volume: (in press)
  • Peer Reviewed
• [Journal Article] A short-term preservation of human cultured periosteal sheets, osteogenic grafting materials, using a comer-cial preservation solution containing epigallocatechin-3-gallate(Thelio-keepR) under chilled conditions (2012)
  • Author(s): Kamiya M, Kawase T, Kobayashi M, Sekine Y, Okuda K, Nagata M, Fuse I, Nakata K, Wolff LF, Yoshie H
  • Journal Title: Biopreserv Biobank Volume: (in press)
  • Peer Reviewed
• [Journal Article] (総説)培養骨膜シート移植を応用した歯周組織再生法 (2012)
  • Author(s): 奥田一博、川瀬知之、中田光、吉江弘正
  • Journal Title: 再生医療(日本再生医療学会雑誌) Volume: 11(1) Pages: 51-56
  • Peer Reviewed
• [Journal Article] A clinical study of the alveolar bone tissue engineering with cultured autogenous periosteal cells : coordinated activation of bone forma-tion and resorption (2012)
  • Author(s): Nagata M, Hoshina H, Li M, Arasawa M, Uematsu K, Ogawa S, Yamada K, Kawase T, Suzuki K, Ogose A, Amizuka N, Fuse I, Okuda K, Uoshima K, Nakata K, Yoshie H, Takagi R
  • Journal Title: Bone Volume: 50(5) Pages: 1123-1129
  • Peer Reviewed
• [Journal Article] An osteogenic grafting complex composed of human periosteal sheet and a porous poly(L-lactic acid) membrane scaffold : Biocompatibility, bio-degradability, and cell fate in vivo (2012)
  • Author(s): Kawase T, Tanaka T, Nishimoto T, Okuda K, Nagata M, Burns DM, Yoshie H
  • Journal Title: J Bioact Compat Polym Volume: 27(2) Pages: 107-121
  • Peer Reviewed
• [Journal Article] 培養骨膜シート移植を応用した歯周組織再生法 (2012)
  • Author(s): 奥田一博、川瀬知之、中田光、吉江弘正
  • Journal Title: 再生医療 Volume: 11 Pages: 51-56
  • Peer Reviewed
• [Journal Article] A clinical study of the alveolar bone tissue engineering with cultured autogenous periosteal cells : coordinated activation of bone formation and resorption (2012)
  • Author(s): Nagata M, Hoshina H, Li M, Arasawa M, Uematsu K, Ogawa S, Yamada K, Kawase T, Suzuki K, Ogose A, Amizuka N, Fuse I, Okuda K, Uoshima K, Nakata K, Yoshie H, Takagi R
  • Journal Title: Bone Volume: 50 Pages: 1123-1129
  • Peer Reviewed
• [Journal Article] A short-term preservation of human cultured periosteal sheets, osteogenic grafting materials, using a commercial preservation solution containing epigallocatechin-3-gallate (Theliokeep^[○!R]) under chilled conditions (2012)
  • Author(s): Kamiya M, Kawase T, Kobayashi M, Sekine Y, Okuda K, Nagata M, Fuse I, Nakata K, Wolff LF, Yoshie H
  • Journal Title: Biopreserv Biobank Volume: (in press)
  • Peer Reviewed
• [Journal Article] An osteogenic grafting complex composed of human periosteal sheet and a porous poly(L-lactic acid) membrane scaffold : Biocom-patibility, biodegradability, and cell fate in vivo (2012)
  • Author(s): Kawase T, Tanaka T, Nishimoto T, Okuda K, Nagata M, Burns DM, Yoshie H
  • Journal Title: J Bioact Compat Polym Volume: 27 Pages: 107-121
  • Peer Reviewed
• [Journal Article] Improved adhesion of human cultured periosteal sheets to a porous poly(L-lactic acid) membrane scaffold without the aid of exogenous adhesion biomolecules (2011)
  • Author(s): Kawase T, Tanaka T, Nishimoto T, Okuda K, Nagata M, Burns DM, Yoshie H
  • Journal Title: J Biomed Mater Res A Volume: 98(7) Pages: 100-113
  • Peer Reviewed
• [Journal Article] Nondestructive microstructural analysis of porous bioceramics by microfocus X-ray computed tomography(μCT): A proposed protocol for standardized evaluation of porosity and interconnectivity between macro-pores (2011)
  • Author(s): Nakayama H, Burns DM, Kawase T
  • Journal Title: J Nondest Eval Volume: 30(2) Pages: 71-80
  • Peer Reviewed
• [Journal Article] Manual cryopreservation of human alveolar periosteal tissue segments : Effects of pre-culture on recovery rate (2011)
  • Author(s): Kawase T, Kogami H, Nagata M, Uematsu K, Okuda K, Burns DM, Yoshie H
  • Journal Title: Cryobiol Volume: 62(3) Pages: 202-209
  • Peer Reviewed
• [Journal Article] In-vivo near-infrared optical imaging of growing osteosar-coma cell lesions xenografted in mice : Dual-channel quantitative evaluation of volume and mineralization (2011)
  • Author(s): Nakayama H, Kawase T, Okuda K, Wolff LF, Yoshie H
  • Journal Title: Acta Radiol Volume: 52(9) Pages: 978-988
  • Peer Reviewed
• [Journal Article] Nondestructive microstructural analysis of porous bioceramics by microfocus X-ray computed tomography (μCT) : A proposed protocol for standardized evaluation of porosity and intercomnectivity between macro-pores (2011)
  • Author(s): Nakayama H, Burns DM, Kawase T
  • Journal Title: J Nondest Eval Volume: 30 Pages: 71-80
  • Peer Reviewed
• [Journal Article] Manual cryopreservation of human alveolar periosteal tissue segments : Effects of pre-culture on recovery rate (2011)
  • Author(s): Kawase T, Kogami H, Nagata M, Uematsu K, Okuda K, Burns DM, Yoshie H
  • Journal Title: Cryobiology Volume: 62 Pages: 202-209
  • Peer Reviewed
• [Journal Article] In-vivo near-infrared optical imaging of growing osteosarcoma cell lesions xenografted in mice : Dual-channel quantitative evaluation of volume and mineralization (2011)
  • Author(s): Nakayama H, Kawase T, Okuda K, Wolff LF, Yoshie H
  • Journal Title: Acta Radiol Volume: 52 Pages: 978-988
  • Peer Reviewed
• [Journal Article] Manual cryopreservation of human alveolar periosteal tissue segments : Effects ofpre-culture on recovery rate. (2011)
  • Author(s): Kawase T, Kogami H, Nagata M, Uematsu K, Okuda K, Burns DM, Yoshie H.
  • Journal Title: Cryobiology Volume: in press
  • Peer Reviewed
• [Journal Article] Nondestructive microstructural analysis of porous bioceramics by microfocusX-ray computed tomography (micro-CT) : A proposed protocol for standardizedevaluation of porosity and interconnectivity between macro-pores. (2011)
  • Author(s): Nakayama H, Burns DM, Kawase T
  • Journal Title: J Nondest Eval Volume: in press
  • Peer Reviewed
• [Journal Article] Improved adhesion of human cultured periosteal sheets to a porous poly(L-lactic acid) membrane scaffold without the aid of exogenous adhesion biomolecules. (2011)
  • Author(s): Kawase T, Tanaka T, Nishimoto T, Okuda K, Nagata M, Burns DM, Yoshie H.
  • Journal Title: J Biomed Mater Res A Volume: in press
  • Peer Reviewed
• [Journal Article] Colla-gen-coated poly(L-lactide-co-ε-caprolactone) film : A promising scaffold for cultured periosteal sheets (2010)
  • Author(s): Kawase T, Yamanaka K, Suda Y, Kaneko T, Okuda K, Kogami H, Nakayama H, Nagata M, Wolff LF, Yoshie H
  • Journal Title: J Periodontol Volume: 81 Pages: 1653-1662
  • Peer Reviewed
• [Journal Article] (総説)歯周組織再生を促す生理活性物質と細胞療法に関するトランスレーショナルリサーチ (2010)
  • Author(s): 川瀬知之
  • Journal Title: 日本歯周病学会誌 Volume: 52(1) Pages: 3-11
  • Peer Reviewed
• [Journal Article] Human periosteum-derived cells combined with superporous hydroxyl-apatite blocks used as an osteogenic bone substitute for periodontal regenerative therapy : Animal implant-tation study using nude mice (2010)
  • Author(s): Kawase T, Okuda K, Kogami H, Nakayama H, Nagata M, Sato T, Wolff LF, Yoshie H
  • Journal Title: J Periodontol Volume: 81(3) Pages: 420-427
  • Peer Reviewed
• [Journal Article] Osteogenic activity of human periosteal sheets cultured on salmon collagen-coated ePTFE meshes (2010)
  • Author(s): Kawase T, Okuda K, Kogami H, Nakayama H, Nagata M, Yoshie H
  • Journal Title: J Mater Sci Mater Med Volume: 21(2) Pages: 731-739
  • Peer Reviewed
• [Journal Article] Evaluation by bone scinti-graphy of osteogenic activity of commercial bioceramics(porousβ-TCP and HAp particles) subcutane-ously implanted in rats (2010)
  • Author(s): Nakayama H, Kawase T, Okuda K, Kogami H, Inoue H, Oda T, Hayama K, Tsuchimo-chi M, Wolff LW
  • Journal Title: J Biomater Appl Volume: 24(8) Pages: 751-768
  • Peer Reviewed
• [Journal Article] Collagen-coated poly(L-lactide-co-ε-caprolactone) film : A promising scaffold forcultured periosteal sheets. (2010)
  • Author(s): Kawase T, Yamanaka K, Suda Y, Kaneko T, Okuda K, Kogami H, Nakayama H, Nagata M, Wolff LF, Yoshie H.
  • Journal Title: J Periodontol Volume: 81 Pages: 1653-1662
  • Peer Reviewed
• [Journal Article] 歯周組織再生を促す生理活性物質と細胞療法に関するトランスレーショナルリサーチ (2010)
  • Author(s): 川瀬知之
  • Journal Title: 日本歯周病学会誌 Volume: 52 Pages: 3-11
  • NAID: 10027096014
  • Peer Reviewed
• [Journal Article] Osteogenic activity of human periosteal sheets cultured on salmon collagen-coated ePTFE meses. (2010)
  • Author(s): Kawase T, Okuda K, Kogami H, Nakayama H, Nagata M, Yoshie H.
  • Journal Title: J Mater Sci Mater Med 21 Pages: 731-739
  • Peer Reviewed
• [Journal Article] Human periosteum-derived cells combined with superporous hydroxyapatite blocks used asan osteogenic bone substitute for periodontal regenerative therapy : Animal implantation study using nude mice. (2010)
  • Author(s): Kawase T, Okuda K, Kogami H, Nakayama H, Nagata M, Sato T, Wolff LF, Yoshie H.
  • Journal Title: J Periodontol 81 Pages: 420-427
  • Peer Reviewed
• [Journal Article] Characterization of human cultured periosteal sheets expressing bone-forming potential : in vitro and in vivo animal studies (2009)
  • Author(s): Kawase T, Okuda K, Kogami H, Nakayama H, Nagata M, Nakata K, Yoshie H
  • Journal Title: J Tissue Eng Reg Med Volume: 3 Pages: 218-229
  • Peer Reviewed
• [Journal Article] (総説)歯肉細胞シート・骨膜シートを用いた歯周再生治療 (2009)
  • Author(s): 吉江弘正、奥田一博、川瀬知之
  • Journal Title: 日本口腔外科学会雑誌 Volume: 55(9) Pages: 432-439
  • Peer Reviewed
• [Journal Article] X線マイクロCTによる生体活性セラミックス多孔体の微小構造解析 (2009)
  • Author(s): 中山均、川瀬知之
  • Journal Title: 歯科放射線 Volume: 49(3) Pages: 33-40
  • NAID: 10027301009
  • Peer Reviewed
• [Journal Article] Evaluation by bone scintigraphy of osteogenic activity of commercial bioceramics (porousβ-TCP and HAp particles) subcutaneously implanted in rats.
  • Author(s): Nakayama H, Kawase T, Okuda K, Kogami H, Inoue H, Oda T, Hayama K, Tsuchimochi M, Wolff LW.
  • Journal Title: J Biomater Appl (in press)
  • Peer Reviewed
• [Presentation] 幹細胞用培地は骨膜シートのポテンシャル向上に貢献するか? (2011)
  • Author(s): 上松晃也、川瀬知之、永田昌毅、奥田一博、吉江弘正、高木律夫
  • Organizer: 第54回秋季日本歯周病学会学術大会
  • Place of Presentation: 広島大、下関
  • Year and Date: 2011-09-24
• [Presentation] 幹細胞用培地は骨膜シートのポテンシャル向上に貢献するか? (2011)
  • Author(s): 上松晃也、川瀬知之、永田昌毅、奥田一博、吉江弘正、高木律夫
  • Organizer: 第54回秋季日本歯周病学会学術大会
  • Place of Presentation: 下関市(海峡メッセ下関)
  • Year and Date: 2011-09-24
• [Presentation] 骨再生のための生体吸収性ポリマーブレンド多孔質膜の作製および特性評価. (2011)
  • Author(s): 高屋朋彰、川瀬知之、田中孝明
  • Organizer: 化学工学会第76年会
  • Place of Presentation: 東京農工大
  • Year and Date: 2011-03-22
• [Presentation] 培養骨膜シートを用いた歯周組織再生療法の3年予後. (2011)
  • Author(s): 奥田一博、川瀬知之、梶 昌美、中田 光、吉江弘正
  • Organizer: 第10回日本再生医療学会
  • Place of Presentation: 京大プラザホテル
  • Year and Date: 2011-03-02
• [Presentation] 骨膜シートによる歯周組織・顎骨の再生療法. (2011)
  • Author(s): 奥田一博、川瀬知之、梶 昌美、中田 光、吉江弘正
  • Organizer: 第10回日本再生医療学会
  • Place of Presentation: 京王プラザホテル
  • Year and Date: 2011-03-02
• [Presentation] マイコプラズマ抗原刺激によるヒト骨膜細胞への影響. (2011)
  • Author(s): 元井奈都紀、梶昌美、布施一郎、川瀬知之、滝澤淳、森尾友宏、中田光
  • Organizer: 第10回日本再生医療学会
  • Place of Presentation: 京王プラザホテル
  • Year and Date: 2011-03-02
• [Presentation] 培養骨膜シートと基材の複合化による厚みのある骨原性移植物の開発. (2011)
  • Author(s): 川瀬知之
  • Organizer: 第10回日本再生医療学会
  • Place of Presentation: 京王プラザホテル
  • Year and Date: 2011-03-01
• [Presentation] Tissue engineered cultured periosteal sheet application to periodontal regeneration : three year results (2011)
  • Author(s): Okuda K, Kawase T, Nagata M, Nakata K, Wolff LF, Yoshie H
  • Organizer: The 97th Annual Meeting of the Amer Acad Periodontol
  • Place of Presentation: Miami Beach, FL, USA
• [Presentation] Freeze-dried platelet-rich plasma(PRP)-coated polyglactin mesh : a promising alternative for wound care and periodontal regenerative therapy (2011)
  • Author(s): Kawase T, Nakajima Y, Okuda K, Wolff LF, Yoshie H
  • Organizer: The 97th Annual Meeting of the Amer Acad Periodontol
  • Place of Presentation: Miami Beach, FL, USA
• [Presentation] 生体吸収性ポリエステル多孔質膜を足場とする骨膜細胞シートの培養 (2011)
  • Author(s): 多田晋一郎、高屋朋彰、川瀬知之、田中孝明
  • Organizer: 化学工学会第43回秋季大会
  • Place of Presentation: 名古屋工業大学、名古屋
• [Presentation] A wound-dressing coated with freeze-dried platelet-rich plasma : Its potent positive effects on wound-healing (2011)
  • Author(s): Nakajima Y, Kawase T, Okuda K, Wolff LF, Yoshie H
  • Organizer: Tissue Eng Regen Med Int Soc-Asia Pacific Chap 2011
  • Place of Presentation: Singapore, Singapore
• [Presentation] Angiogenic effects of human platelet-rich fibrin(PRF) in chicken chorioallantoic membranes (2011)
  • Author(s): Kobayashi M, Kawase T, Okuda K, Wolff LF, Yoshie H
  • Organizer: Tissue Eng Regen Med Int Soc-Asia Pacific Chap 2011
  • Place of Presentation: Singapore, Singapore
• [Presentation] An osteogenic grafting complex composed of human periosteal sheet and its scaffolding material, a porous poly(L-lactic acid) membrane : biocompatibility, biodegradation, and cell fate in vivo (2011)
  • Author(s): Kawase T, Tanaka T, Nishimoto T, Okuda K, Nagata M, Burns DM, Yoshie H
  • Organizer: Tissue Eng Regen Med Int Soc-Asia Pacific Chap 2011
  • Place of Presentation: Singapore, Singapore
• [Presentation] 培養骨膜シートを用いた歯周組織再生療法の3年予後 (2011)
  • Author(s): 奥田一博、川瀬知之、山宮かの子、吉江弘正
  • Organizer: 第54回春季日本歯周病学会学術大会
  • Place of Presentation: 福岡歯科大、福岡
• [Presentation] 治療用ヒト培養骨膜シートの凍結保存法 (2011)
  • Author(s): 川瀬知之、小神浩幸、永田昌毅、上松晃也、奥田一博、吉江弘正
  • Organizer: 第54回春季日本歯周病学会学術大会
  • Place of Presentation: 福岡歯科大、福岡
• [Presentation] 骨膜シートによる歯周組織・顎骨の再生療法 (2011)
  • Author(s): 吉江弘正、奥田一博、川瀬知之、永田昌毅
  • Organizer: シンポジウム「再生と再建」第28回日本医学会総会
  • Place of Presentation: 東京
• [Presentation] 骨再生のための生体吸収性ポリマーブレンド多孔質膜の作製および特性評価 (2011)
  • Author(s): 高屋朋彰、川瀬知之、田中孝明
  • Organizer: 化学工学会第76年会
  • Place of Presentation: 東京農工大、東京
• [Presentation] 培養骨膜シートを用いた歯周組織再生療法の3年予後 (2011)
  • Author(s): 奥田一博、川瀬知之、梶昌美、中田光、吉江弘正
  • Organizer: 第10回日本再生医療学会
  • Place of Presentation: 東京女子医大、東京
• [Presentation] 骨膜シートによる歯周組織・顎骨の再生療法.シンポジウム「歯周組織はどこまで再生するか」 (2011)
  • Author(s): 吉江弘正、奥田一博、川瀬知之、永田昌毅、中田光
  • Organizer: 第10回日本再生医療学会
  • Place of Presentation: 東京女子医大、東京
• [Presentation] 培養骨膜シートと基材の複合化による厚みのある骨原性移植物の開発.シンポジウム「細胞3次元構築を目指した組織工学」 (2011)
  • Author(s): 川瀬知之
  • Organizer: 第10回日本再生医療学会
  • Place of Presentation: 東京女子医大、東京
• [Presentation] Tissue engineered cultured periosteal sheet application to periodontal regeneration : three year results (2011)
  • Author(s): Okuda K, Kawase T, Nagata M, Nakata K, Wolff LF, Yoshie H
  • Organizer: The 97th Annual Meeting of the American Academy of Periodontology
  • Place of Presentation: Miami Beach, FL, USA (Miami Beach Convention Center)
• [Presentation] Freeze-dried platelet-rich plasma (PRP)-coated polyglactin mesh : a promising alternative for wound care and periodontal regenerative therapy (2011)
  • Author(s): Kawase T, Nakajima Y, Okuda K, Wolff LF, Yoshie H
  • Organizer: The 97th Annual Meeting of the American Academy of Periodontology
  • Place of Presentation: Miami Beach, FL, USA (Miami Beach Convention Center)
• [Presentation] 生体吸収性ポリエステル多孔質膜を足場とする骨膜細胞シートの培養 (2011)
  • Author(s): 多田晋一郎、高屋朋彰、川瀬知之、田中孝明
  • Organizer: 化学工学会第43回秋季大会
  • Place of Presentation: 名古屋市(名古屋工業大学)
• [Presentation] A wound-dressing coated with freeze-dried platelet-rich plasma : Its potent positive effects on wound-healing (2011)
  • Author(s): Nakajima Y, Kawase T, Okuda K, Wolff LF, Yoshie H
  • Organizer: Tissue Engineering and Regenerative Medicine International Society-Asia Pacific Chapter 2011
  • Place of Presentation: Singapore (Waterfront Conference Centre)
• [Presentation] Angiogenic effects of human platelet-rich fibrin (PRF) in chicken chorioallantoic membranes (2011)
  • Author(s): Kobayashi M, Kawase T, Okuda K, Wolff LF, Yoshie H
  • Organizer: Tissue Engineering and Regenerative Medicine International Society-Asia Pacific Chapter 2011
  • Place of Presentation: Singapore (Waterfront Conference Centre)
• [Presentation] An osteogenic grafting coraplex composed of human periosteal sheet and its scaffolding material, a porous poly(L-lactic acid) membrane : biocompatibility, biodegradation, and cell fate in vivo (2011)
  • Author(s): Kawase T, Tanaka T, Nishimoto T, Okuda K, Nagata M, Burns DM, Yoshie H
  • Organizer: Tissue Engineering and Regenerative Medicine International Society-Asia Pacific Chapter 2011
  • Place of Presentation: Singapore (Waterfront Conference Centre)
• [Presentation] 培養骨膜シートと基材の複合化による厚みのある骨原性移植物の開発 (2011)
  • Author(s): 川瀬知之
  • Organizer: 第44回日本整形外科学会骨・軟骨腫瘍学術集会
  • Place of Presentation: 京都市(国立京都国際会館)(招待講演)
• [Presentation] 培養骨膜シートを用いた歯周組織再生療法の3年予後 (2011)
  • Author(s): 奥田一博、川瀬知之、山宮かの子、吉江弘正
  • Organizer: 第54回春季日本歯周病学会学術大会
  • Place of Presentation: 福岡市(福岡国際会議場)
• [Presentation] 治療用ヒト培養骨膜シートの凍結保存法 (2011)
  • Author(s): 川瀬知之、小神浩幸、永田昌毅、上松晃也、奥田一博、吉江弘正
  • Organizer: 第54回春季日本歯周病学会学術大会
  • Place of Presentation: 福岡市(福岡国際会議場)
• [Presentation] 骨膜シートによる歯周組織・顎骨の再生療法 (2011)
  • Author(s): 吉江弘正、奥田一博、川瀬知之、永田昌毅
  • Organizer: 第28回日本医学会総会
  • Place of Presentation: (招待講演)(震災のためWEB開催に変更)
• [Presentation] 体活性セラミックスの特性評価に関する標準化(1)気孔構造の分析方法。 (2010)
  • Author(s): 名井陽、川崎健史、森本健司、服部昌晃、浅岡伸之、井上晃、井村浩一、宮路史明、植松敬三、橋本雅美、川瀬知之、菊池正紀
  • Organizer: 第32回日本バイオマテリアル学会
  • Place of Presentation: グランドプリンスホテル広島
  • Year and Date: 2010-11-30
• [Presentation] 多血小板フィブリン(PRF)の創傷治癒に及ぼす効果 (2010)
  • Author(s): 中島悠、川瀬知之、奥田一博、吉江弘正
  • Organizer: 第53回秋季日本歯周病学会学術大会
  • Place of Presentation: 徳島大、高松
  • Year and Date: 2010-09-19
• [Presentation] 多血小板フィブリン(PRF)の創傷治癒に及ぼす効果. (2010)
  • Author(s): 中島悠、川瀬知之、奥田一博、吉江弘正
  • Organizer: 第53回秋季日本歯周病学会学術大会
  • Place of Presentation: サンポートホール高松&かがわ国際会議場
  • Year and Date: 2010-09-19
• [Presentation] 創傷治癒・組織再生に有効な生体活性成分を含む基材 (2010)
  • Author(s): 川瀬知之
  • Organizer: 新技術説明会
  • Place of Presentation: 新潟大学、東京
  • Year and Date: 2010-07-30
• [Presentation] 骨膜片採取から移植治療用培養骨膜シート形成に至る過程での細胞動態の分析. (2010)
  • Author(s): 奥田一博、川瀬知之、小神浩幸、永田昌毅、吉江弘正
  • Organizer: 第53回春季日本歯周病学会学術大会
  • Place of Presentation: 盛岡市民文化ホール
  • Year and Date: 2010-05-14
• [Presentation] 異所性骨形成モデルにおけるNIR(近赤外線)検査の応用の試み (2010)
  • Author(s): 中山均、川瀬知之、小神浩幸、奥田一博、吉江弘正.
  • Organizer: 第9回日本再生医療学会
  • Place of Presentation: 広島市
  • Year and Date: 2010-03-19
• [Presentation] 培養自家骨膜シートをもちいた歯周組織再生療法に関する基礎的研究 (2010)
  • Author(s): 川瀬知之
  • Organizer: 第9回日本再生医療学会
  • Place of Presentation: 広島市
  • Year and Date: 2010-03-18
• [Presentation] ポリ乳酸カプロラクトン重合体フィルムのヒト骨膜シート培養・移植への応用 (2010)
  • Author(s): 奥田一博、川瀬知之、山中克之、須田洋子、金子正、小神浩幸、中山均、永田昌毅、吉江弘正.
  • Organizer: 第9回日本再生医療学会
  • Place of Presentation: 広島市
  • Year and Date: 2010-03-18
• [Presentation] Clinical and histologic evaluation of tissue-engineered cultured periosteum application for bone regeneration (2010)
  • Author(s): Okuda K, Yoshie H, Kawase T, Kogami H, Nakayama H, Nagata M, Takagi R
  • Organizer: The 96th Annual Meeting of the Amer Acad Periodontol
  • Place of Presentation: Honolulu, HI, USA
• [Presentation] Non-invasive evaluation of the osteogenic activity of alveolar bone-derived human periosteal sheets in animal implantation models by in vivo near-infrared(NIR) fluorescence optical imaging (2010)
  • Author(s): Kawase T, Nakayama H, Okuda K, Kogami H, Nagata M, Wolff LF, Yoshie H
  • Organizer: The 96th Annual Meeting of the Amer Acad Periodontol
  • Place of Presentation: Honolulu, HI, USA
• [Presentation] 培養細胞による非晶質水酸アパタイト骨充填材の製造法 (2010)
  • Author(s): 川瀬知之
  • Organizer: イノベーション・ジャパン2010-大学見本市
  • Place of Presentation: JST, NEDO、東京
• [Presentation] A porous poly(L-lactic acid) membrane designed for culturing human perios-teal sheets as an osteogenic grafting material (2010)
  • Author(s): Kawase T, Tanaka T, Nishimoto T, Okuda K, Nagata M, Burns DM, Yoshie H
  • Organizer: Tissue Eng Regen Med Int Soc-Asia Pacific Chap 2010
  • Place of Presentation: Sydney, Australia
• [Presentation] 骨膜片採取から移植治療用培養骨膜シート形成に至る過程での細胞動態の分析 (2010)
  • Author(s): 奥田一博、川瀬知之、小神浩幸、永田昌毅、吉江弘正
  • Organizer: 第53回春季日本歯周病学会学術大会
  • Place of Presentation: 岩手医大、盛岡
• [Presentation] 歯周組織再生を促す生理活性物質と細胞療法に関するトランスレーショナルリサーチ (2010)
  • Author(s): 川瀬知之
  • Place of Presentation: 岩手医大、盛岡
• [Presentation] 異所性骨形成モデルにおけるNIR(近赤外線)検査の応用の試み (2010)
  • Author(s): 中山均、川瀬知之、小神浩幸、奥田一博、吉江弘正
  • Organizer: 第9回日本再生医療学会
  • Place of Presentation: 広島大、広島
• [Presentation] 培養自家骨膜シートをもちいた歯周組織再生療法に関する基礎的研究.シンポジウム「歯科領域の再生医療」 (2010)
  • Author(s): 川瀬知之
  • Organizer: 第9回日本再生医療学会
  • Place of Presentation: 広島大、広島
• [Presentation] Clinical and histolgic evaluation of tissue-engineered cultured periosteumapplication for bone regeneration. (2010)
  • Author(s): Okuda K, Yoshie H, Kawase T, Kogami H, Nakayama H, Nagata M, Takagi R.
  • Organizer: The 96th Annual Meeting of the American Academy ofPeriodontology
  • Place of Presentation: Honolulu Convention Center(Hawaii, USA)
• [Presentation] Non-invasive evaluation of the osteogenic activity of alveolar bone-derived humanperiosteal sheets in animal implantation models by in vivo near-infrared(NIR)fluorescence optical imaging. (2010)
  • Author(s): Kawase T, Nakayama H, Okuda K, Kogami H, Nagata M, Wolff LF, Yoshie H.
  • Organizer: The 96th Annual Meeting of the American Academy ofPeriodontology
  • Place of Presentation: Honolulu Convention Center(Hawaii, USA)
• [Presentation] Aporous poly(L-lactic acid)membrane designed for culturing human periosteal sheets as an osteogenic grafting material. (2010)
  • Author(s): Kawase T, Tanaka T, Nishimoto T, Okuda K, Nagata M, Burns DM, Yoshie H.
  • Organizer: Termis-AP 2010(Tissue Engineering and Regenerative Medicine International Society-Asia Pacific Meeting 2011)
  • Place of Presentation: Sheraton on the park Sydney(Australia)
• [Presentation] 歯周組織再生を促す生理活性物質と細胞療法に関するトランスレーショナルリサーチ (2009)
  • Author(s): 川瀬知之
  • Organizer: 第52回日本歯周病学会秋季学術大会
  • Place of Presentation: 九州大、宮崎
  • Year and Date: 2009-10-11
• [Presentation] インプラント症例を対象とした培養自家骨膜による歯槽骨再生 (2009)
  • Author(s): 永田昌毅、川瀬知之、吉江弘正、奥田一博、中田光、高木律男
  • Organizer: 第52回日本歯周病学会秋季学術大会
  • Place of Presentation: 九州大、宮崎
  • Year and Date: 2009-10-11
• [Presentation] インプラント症例を対象とした培養自家骨膜による歯槽骨再生 (2009)
  • Author(s): 永田昌毅、川瀬知之、吉江弘正、奥田一博、中田光、高木律男.
  • Organizer: 第52回日本歯周病学会秋季学術大会
  • Place of Presentation: 宮崎市
  • Year and Date: 2009-10-11
• [Presentation] 歯科インプラントを目的とした培養自家骨膜併用による歯槽骨再生 (2009)
  • Author(s): 永田昌毅、星名秀行、川瀬知之、荒澤恵、山田一穂、嵐山貴徳、中田光、高木律男
  • Organizer: 日本形成外科学会関東支部第80回新潟地方会
  • Place of Presentation: 新潟大歯、新潟
  • Year and Date: 2009-07-13
• [Presentation] ハイドロキシアパタイト多孔体を足場にした培養骨による異所性骨形成 -非侵襲的近赤外蛍光イメージング評価法の可能性- (2009)
  • Author(s): 川瀬知之、中山均、小神浩幸、奥田一博、吉江弘正.
  • Organizer: 第52回日本歯周病学会春季学術大会
  • Place of Presentation: 岡山市
  • Year and Date: 2009-05-15
• [Presentation] The human cultured periosteal sheet for peri-odontal regeneration : A salmon collagen-coated mesh, a functional potent scaffold, to upregulate the osteogenic potential (2009)
  • Author(s): Okuda K, Yoshie H, Kawase T, Kogami H, Nakayama H, Nagata M
  • Organizer: The 95th Annual Meeting of the Amer Acad Periodontol
  • Place of Presentation: Boston, MA, USA
• [Presentation] The human cultured periosteal sheet for periodontal regeneration : A salmon collagen-coatedmesh, a functional potent scaffold, to upregulate the osteogenic potential. (2009)
  • Author(s): Okuda K, Yoshie H, Kawase T, Kongami H, Nakayama H, Nagata M.
  • Organizer: The 95th Annual Meeting of the American Academy of Periodontology
  • Place of Presentation: Boston, MA, USA
• [Book] 凍結乾燥PRPコート創傷被覆材の開発.第6章医薬品応用,『化粧品・医薬品における次世代経皮吸収製剤』 (2011)
  • Author(s): 川瀬知之,杉林堅次
  • Publisher: 株式会社シーエムシー
• [Book] 化粧品・医薬品における次世代経皮吸収製剤 (2011)
  • Author(s): 川瀬知之
  • Publisher: 株式会社シーエムシー出版
• [Book] 第3章細胞の利用2.前駆細胞、幹細胞10)培養骨膜シート移植を応用した歯周組織再生治療.遺伝子医学MOOK13患者までとどいている再生誘導治療-バイオマテリアル、生体シグナル因子、細胞を利用した患者のための再生医療の実際- (2009)
  • Author(s): 奥田一博、川瀬知之、山宮かの子、吉江弘正、田畑泰彦
  • Publisher: 株式会社メディカルドゥ
• [Patent(Industrial Property Rights)] フィブリンゲル圧延装置 (2012)
  • Inventor(s): 山内慶次郎、阿部和幸、川瀬知之
  • Industrial Property Rights Holder: YSEC株式会社、新潟大学
  • Filing Date: 2012-01-13
  • Overseas
• [Patent(Industrial Property Rights)] フィブリンゲル圧延装置 (2012)
  • Inventor(s): 山内慶次郎、阿部和幸、川瀬知之
  • Industrial Property Rights Holder: YSEC株式会社、新潟大学
  • Filing Date: 2012-01-13
  • Overseas
• [Patent(Industrial Property Rights)] フィブリンゲル圧延装置 (2011)
  • Inventor(s): 山内慶次郎、阿部和幸、川瀬知之
  • Industrial Property Rights Holder: YSEC株式会社、新潟大学
  • Acquisition Date: 2011-07-01
• [Patent(Industrial Property Rights)] フィブリンゲル圧延装置 (2011)
  • Inventor(s): 山内慶次郎、阿部和幸、川瀬知之
  • Industrial Property Rights Holder: YSEC株式会社、新潟大学
  • Filing Date: 2011-07-01
  • Acquisition Date: 2011-01-28
• [Patent(Industrial Property Rights)] 培養細胞が産生する石灰化物による骨形成・再生 (2010)
  • Inventor(s): 川瀬知之
  • Industrial Property Rights Holder: 新潟大学
  • Filing Date: 2010-03-05
  • Overseas
• [Patent(Industrial Property Rights)] ヒト骨膜培養方法 (2010)
  • Inventor(s): 川瀬知之、奥田一博
  • Industrial Property Rights Holder: 新潟大学
  • Industrial Property Number: 2009-529083
  • Filing Date: 2010-01-26
  • Overseas
• [Patent(Industrial Property Rights)] 培養細胞が産生する石灰化物による骨形成・再生 (2010)
  • Inventor(s): 川瀬知之
  • Industrial Property Rights Holder: 新潟大学
  • Filing Date: 2010-03-05
  • Overseas
• [Patent(Industrial Property Rights)] 再生治療用材料 (2009)
  • Inventor(s): 川瀬知之、奥田一博、中島悠
  • Industrial Property Rights Holder: 新潟大学
  • Industrial Property Number: 2009-281382
  • Filing Date: 2009-12-11
• [Patent(Industrial Property Rights)] 再生医療用材料 (2009)
  • Inventor(s): 川瀬知之、奥田一博、中島悠
  • Industrial Property Rights Holder: 新潟大学
  • Industrial Property Number: 2009-281382
  • Filing Date: 2009-12-11