The search of graded epigenetic change of oral cancer and Neighboring epithelial dysplasia

• Project/Area Number: 21592519
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Niigata University
• Principal Investigator: FUJITA Hajime 新潟大学, 歯学部, 非常勤講師 (60271805)
• Co-Investigator(Kenkyū-buntansha): TAKAGI Ritsuo 新潟大学, 医歯学系, 教授 (20143795) ; HOSHINA Hideyuki 新潟大学, 医歯学総合病院, 講師 (30173587) ; NAGATA Masaki 新潟大学, 医歯学総合病院, 講師 (10242439) ; IKEDA Nobuyuki 新潟大学, 医歯学系, 助教 (70419282)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 口腔扁平上皮癌 / 異型上皮 / エピジェネティクス / DNAメチル化 / 多段階発癌

Research Abstract

We obtained a tissue specimen of normal tissue(N), epithelial dysplasia(D) and cancer(C), and extracted genomic DNA from the sample of two carcinoma of mandibular gingiva. We analyzed DNA methylation using illumina HumanMethylation27 and conducted the statistical analysis of the results. The gene which a methylation level rose to progressively in N group→D group→C group was EPDR1, FOXL1, FLJ21159, HOXB4, PRAC, DAPK1, FLJ46156, WDR8, BARHL2, NFAM1. Conversely, the gene which a methylation level decreased progressively was MEGEA3, GJB7, MFAP2, GPLD1, LOC348645, MEGEA6, FCN2, CCL1, SLC24A2, C9orf84. For the cancellation of the individual difference, it will be necessary to increase cases in future.

Research Products

• [Journal Article] Assessment of 14 functional gene polymorphisms in Japanese patients with oral lichen planus : a pilot case-control study (2009)
  • Author(s): Fujita H, Kobayashi T, Tai H, Nagata M, Hoshina H, Nishizawa R, Takagi R, Yoshie H
  • Journal Title: Int J Oral Maxillofac Surg Volume: 38(9) Pages: 978-983
  • Peer Reviewed
• [Journal Article] Tetraspanin gene expression levels as potential biomarkers for malignancy of gingival squamous cell carcinoma (2009)
  • Author(s): Hirano C, Nagata M, Noman A, Kitamura N, Ohnishi M, Ohyama T, Suzuki K, Yoshizawa K, Izumi N, Fujita H, Takagi R
  • Journal Title: Int J Cancer Volume: 124 Pages: 2911-2916
  • Peer Reviewed
• [Presentation] 下顎骨エナメル上皮腫・腺様歯原性腫瘍ハイブリッド腫瘍の1例 (2010)
  • Author(s): 藤田一,池田順行,齋藤太郎,高木律男,林孝文,山崎学,朔敬
  • Organizer: 第55回日本口腔外科学会総会
  • Place of Presentation: 千葉市