2011 Fiscal Year Final Research Report

The search of graded epigenetic change of oral cancer and Neighboring epithelial dysplasia

Research Project

Project/Area Number	21592519
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Surgical dentistry
Research Institution	Niigata University
Principal Investigator	FUJITA Hajime 新潟大学, 歯学部, 非常勤講師 (60271805)
Co-Investigator(Kenkyū-buntansha)	TAKAGI Ritsuo 新潟大学, 医歯学系, 教授 (20143795) HOSHINA Hideyuki 新潟大学, 医歯学総合病院, 講師 (30173587) NAGATA Masaki 新潟大学, 医歯学総合病院, 講師 (10242439) IKEDA Nobuyuki 新潟大学, 医歯学系, 助教 (70419282)
Project Period (FY)	2009 – 2011
Keywords	口腔扁平上皮癌 / 異型上皮 / エピジェネティクス / DNAメチル化 / 多段階発癌
Research Abstract	We obtained a tissue specimen of normal tissue(N), epithelial dysplasia(D) and cancer(C), and extracted genomic DNA from the sample of two carcinoma of mandibular gingiva. We analyzed DNA methylation using illumina HumanMethylation27 and conducted the statistical analysis of the results. The gene which a methylation level rose to progressively in N group→D group→C group was EPDR1, FOXL1, FLJ21159, HOXB4, PRAC, DAPK1, FLJ46156, WDR8, BARHL2, NFAM1. Conversely, the gene which a methylation level decreased progressively was MEGEA3, GJB7, MFAP2, GPLD1, LOC348645, MEGEA6, FCN2, CCL1, SLC24A2, C9orf84. For the cancellation of the individual difference, it will be necessary to increase cases in future.