Testing the utility of rationally engineered recombinant collagen-like proteins for applications of bone regeneration with bone morphogenetic protein in tissue engineering.

• Project/Area Number: 21592520
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Nagoya University
• Principal Investigator: HATTORI Hisashi 名古屋大学, 医学部附属病院, 講師 (60332699)
• Co-Investigator(Kenkyū-buntansha): ITOH Kenji 名古屋大学, 医学部附属病院, 助教 (50402623) ; KINOSHITA Kazuhiko 名古屋大学, 医学部附属病院, 医員 (40467296) ; UEDA Minoru 名古屋大学, 大学院・医学系研究科, 教授 (00151803)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: リコンビナント / cDNAカセット / コラーゲン / 骨形成蛋白(BMP) / 骨再生 / スキャフォールド / 異所性骨化

Research Abstract

We mapped the D4 domain of human collagen I as most critical for binding site of bone morphogenetic protein(BMP) and used this information to genetically engineer a collagen-like protein consisting of tandem repeats of the D4 domain(mD4 collagen). We proved recombinant human mD4 collagen-like protein had a potential for an efficient scaffold as same as other scaffolds materials for engineering of bone when combined with BMP.

Research Products

• [Journal Article] Effects of self-assembling peptidehydrogel scaffold on bone regeneration with recombinant human bone morphogeneticprotein-2 (2011)
  • Author(s): Ikeno M, Hibi H, Kinoshita K, Hattori H, Ueda M
  • Journal Title: Oral & Craniofac Tis Eng Volume: Vol.1(2) Pages: 91-97
  • Peer Reviewed
• [Journal Article] Effects of the permeability of shields with autologous bone grafts on bone augmentation (2011)
  • Author(s): Ikeno M, Hibi H, Kinoshita K, Hattori H, Ueda M
  • Journal Title: Oral & Craniofac Tis Eng Volume: Vol.1(3) Pages: 198-204
  • Peer Reviewed
• [Journal Article] Effects of self-assembling peptide hydrogel scaffold on bone regeneration with recombinant human bone morphogenetic protein-2 (2011)
  • Author(s): Ikeno, et al
  • Journal Title: Oral & Craniofacial Tissue ENGINEERING Volume: Vol.1(2) Pages: 91-97
  • Peer Reviewed
• [Journal Article] Effects of the permeability of shields with autologous bone grafts on bone augmentation (2011)
  • Author(s): Ikeno, et al
  • Journal Title: Oral & Craniofacial Tissue ENGINEEEING Volume: Vol.1(3) Pages: 198-204
  • Peer Reviewed
• [Presentation] Testing the utility of rationally engineered recombinant collagen-like proteins for applications of bone regeneration in tissue engineering (2010)
  • Author(s): 服部宇
  • Organizer: International Society for Cellular ther-apy Europe Meeting
  • Place of Presentation: Belgira-te, Italy
  • Year and Date: 2010-09-11
• [Presentation] Testing the utility of rationally engineered recombinant collagen-like proteins for applications of bone regeneration in tissue engineering (2010)
  • Author(s): 服部宇
  • Organizer: International Society for Cellular therapy Europe Meeting
  • Place of Presentation: Belgirate, Italy
  • Year and Date: 2010-09-11
• [Presentation] Testing the utility of rationally engineered recombinant collagen-like proteins for applications of bone regeneration in tissue engineering (2009)
  • Author(s): 服部宇
  • Organizer: World conference on regenerative medicine
  • Place of Presentation: Leipzig, Germany
  • Year and Date: 2009-11-01
• [Presentation] 機能性リコンビナントコラーゲンの基礎的研究第2報スキャフォールドとしての検討 (2009)
  • Author(s): 服部宇
  • Organizer: 第54回日本口腔外科学会総会
  • Place of Presentation: 札幌
  • Year and Date: 2009-10-10
• [Presentation] 機能性リコンビナントコラーゲンの基礎的研究 第2報スキャフォールドとしての検討 (2009)
  • Author(s): 服部宇
  • Organizer: 第54回日本口腔外科学会総会
  • Place of Presentation: 札幌
  • Year and Date: 2009-10-10
• [Presentation] Ectopic bone for mation inducedby implantation of stem cells by sonichedgehog gene-transduction with recombinant collagen-like protein scaffolds (2009)
  • Author(s): 服部宇
  • Organizer: Termis 2^<nd> World Congress
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2009-09-02
• [Presentation] Ectopic bone formation induced by implantation of stem cells by soiched-gehog gene-transduction with recombinant collagen-like protein scaffolds (2009)
  • Author(s): 服部宇
  • Organizer: Termis 2^<nd> World Congress
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2009-09-02