Induction of tooth and craniofacial tissue from undifferentiated presumptive ectodermal Xenopus cell by aggregation culture method.

• Project/Area Number: 21592527
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Hiroshima University
• Principal Investigator: FUKUI Yasuto 広島大学, 大学院・医歯薬学総合研究科, 助教 (90363085)
• Co-Investigator(Kenkyū-buntansha): OKAMOTO Tetsuji 広島大学, 大学院・医歯薬学総合研究科, 教授 (00169153) ; TORATANI Shigeaki 広島大学, 病院, 講師 (90172220)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: アニマルキャップ / 歯牙誘導 / 顎骨誘導

Research Abstract

We used ectodermal Xenopus cell by aggregation culture method. Pas/ Alcian blue stained positive cartilage tissue was observed in the aggregate. Next, we implanted the aggregate into the abdominal region of Xenopus embryo. We observed PAS/ Alcian blue positive and Amelogenin positive tooth germ-like tissue in transplanted tissue after the host transformation. More, we implanted the aggregate to the embryo(St23) was exicised region of lower jaw, we were not able to observe Pas/ Alcian blue stained positive cartilage tissue.

Research Products

• [Journal Article] 広島大学病院顎・口腔外科における過去14年間の顎矯正手術の臨床統計的検討 (2011)
  • Author(s): 虎谷茂昭、福井康人、岡本哲治ら
  • Journal Title: 広島大学歯学雑誌 Volume: 43 Pages: 9197-9197
  • NAID: 40019200332
  • Peer Reviewed
• [Journal Article] 広島大学病院顎・口腔外科における過去14年間の顎矯正手術の臨床統計的検討 (2011)
  • Author(s): 虎谷茂昭、谷亮治、福井康人, ら
  • Journal Title: 広島大学歯学雑誌 Volume: 43 Pages: 91-97
  • NAID: 40019200332
  • Peer Reviewed
• [Journal Article] オトガイ部知覚異常を初発症状とした側頭下窩に進展した筋肉内脂肪腫の1例 (2009)
  • Author(s): 福井康人、虎谷茂昭、他4名
  • Journal Title: 日本口腔外科学会雑誌 Volume: 55巻 Pages: 580-584
  • NAID: 10027091398
  • Peer Reviewed
• [Journal Article] オトガイ部知覚異常を初発症状とした側頭下窩に進展した筋肉内脂肪腫の1例 (2009)
  • Author(s): 福井康人, 虎谷茂昭, 他4名
  • Journal Title: 日本口腔外科学会雑誌 55 Pages: 580-584
  • NAID: 10027091398
  • Peer Reviewed
• [Presentation] 口蓋に生じた多形腺腫由来筋上皮癌の一例 (2011)
  • Author(s): 福井康人、虎谷茂昭、岡本哲治ら
  • Organizer: 第56回日本口腔外科学会総会・学術大会
  • Place of Presentation: 大阪
  • Year and Date: 2011-10-21
• [Presentation] ガンマナイフが著効した難治性三叉神経痛の一例 (2009)
  • Author(s): 福井康人、吉岡幸男、岡本哲治ら
  • Organizer: 日本口腔科学会学術集会
  • Place of Presentation: 浜松
  • Year and Date: 2009-04-17
• [Presentation] ガンマナイフが著効した難治性三叉神経痛の一例 (2009)
  • Author(s): 福井康人, 吉岡幸男, 他2名
  • Organizer: 日本口腔科学会学術集会
  • Place of Presentation: アクトシティ浜松
  • Year and Date: 2009-04-17