Induction of tooth and craniofacial tissue from undifferentiated presumptive ectodermal Xenopus cell by aggregation culture method.
| Project/Area Number |
21592527
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Surgical dentistry
|
|---|
| Research Institution | Hiroshima University |
|---|
Principal Investigator |
FUKUI Yasuto 広島大学, 大学院・医歯薬学総合研究科, 助教 (90363085)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
OKAMOTO Tetsuji 広島大学, 大学院・医歯薬学総合研究科, 教授 (00169153)
TORATANI Shigeaki 広島大学, 病院, 講師 (90172220)
|
|---|
| Project Period (FY) |
2009 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
|
|---|
| Keywords | アニマルキャップ / 歯牙誘導 / 顎骨誘導 |
|---|
| Research Abstract |
We used ectodermal Xenopus cell by aggregation culture method. Pas/ Alcian blue stained positive cartilage tissue was observed in the aggregate. Next, we implanted the aggregate into the abdominal region of Xenopus embryo. We observed PAS/ Alcian blue positive and Amelogenin positive tooth germ-like tissue in transplanted tissue after the host transformation. More, we implanted the aggregate to the embryo(St23) was exicised region of lower jaw, we were not able to observe Pas/ Alcian blue stained positive cartilage tissue.
|
Report
(4 results)
Research Products
(7 results)
