| Project/Area Number |
21592663
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Social dentistry
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| Research Institution | Nihon University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KOMURO Toshinobu 日本大学, 歯学部, 教授 (50139200)
IZAWA Hikaru 日本大学, 歯学部, 助教 (30514103)
MARUYAMA Sayaka 日本大学, 歯学部, 助教 (30366190)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Keywords | 歯科法医学 / DNA多型解析 / 蛍光消光現象 / ABO式遺伝子型検査 / QProbe / Rh式遺伝子 / 性別判定 / RHCE遺伝子 / SNPタイピング / Melting curve |
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| Research Abstract |
We used "quenching phenomenon" that a fluorescence degree decreased when a QProbe hybridize to a target gene, the fluorescence is quenched via the interaction between the dye and guanine base, and the detection typed SNP by the possible QP(Quenching Probe/Primer) method at the same time of the plural gene and examined the blood genotyping method.
Furthermore, we tried ABO blood group genotyping and sex determination, ABO blood group genotyping and Rh-D and RH-type blood genotyping by multiplex PCR and established those laboratory procedure.
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