MECHANISMS OF BLADDER EP1THELIUM INDUCE PAIN SENSATION

• Project/Area Number: 21600001
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 疼痛学
• Research Institution: Akita University
• Principal Investigator: KAWATANI Masahito 秋田大学, 大学院・医学系研究, 教授 (00177700)
• Co-Investigator(Kenkyū-buntansha): MIYAI Kazumasa 秋田大学, 大学院・医学系研究科, 准教授 (60283933) ; ITO Tomoko 秋田大学, 大学院・医学系研究科, 教授 (50241675) ; YOSHIZUMI Masaru 秋田大学, 大学院・医学系研究科, 助教 (70553379)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 膀胱痛 / TRPV1 / ATP / 上皮細胞 / 膀胱 / 痛み / パルスレーザー照射 / 炎症 / 頻尿 / ノルアドレナリン / 脊髄 / 排尿中枢 / プロスタグランジン / 求心性神経線維

Research Abstract

ATP secretion from the urinary bladder was observed pressure dependently (from 5cmH_2O to 45cmH_2O). Stimulation of either TRPV1 channel, EP1 receptor (Prostaglandin E_2 receptor) or muscarinic receptor increased ATP secretion which releasing Ca^<2+> from endoplastic reticulum. Activation of adenylate cyclase also increased ATP secretion which independent from previous mechanism. Store-operated Ca^<2+> entry suppressed distention-induced ATP release from urothelim. These could be important for continuous ATP release during the storage of the urine in the urinary bladder.

Research Products

• [Journal Article] The regulation of distention-induced ATP release from urotheliumby the adenylyl cyclase-cyclic AMP pathway (2012)
  • Author(s): Matsumoto-Miyai K, Kawatani M
  • Journal Title: Biomedical Res Volume: (in press)
• [Journal Article] 膀胱上皮からのATP分泌における貯蔵量作動性Ca^<2+>流入とCa^<2+>抑制性アデニル酸シクラーゼの役割 (2012)
  • Author(s): 宮井-松本和政, 河谷正仁
  • Journal Title: 排尿プラクティス Volume: 20 Pages: 16-23
• [Journal Article] The regulation of distention-induced ATP release from urotheliumby the adenylyl cyclase-cyclic AMP pathway (2012)
  • Author(s): Matsumoto-Miyai K
  • Journal Title: Biomedical Res Volume: (in press)
  • Peer Reviewed
• [Journal Article] Store-operated Ca^<2+> entry suppresses distention-induced ATP release from urothelium (2011)
  • Author(s): Matsumoto-Miyai K, Kagase A, Yamada E, Yoshizumi M, Murakami M, Ohba M, Kawatani M
  • Journal Title: Am J Physiol Volume: 300
• [Journal Article] Pulse diode laser irradiation (830 nm) of lumbosacral spinal rootsdimnished hyperreflexia-induced by acetic acid or prostaglandin E2 infusion in rat urinary bladder (2011)
  • Author(s): Ito T, Ishikawa T, Matsumoto-Miyai K, Kawatani M
  • Journal Title: Luts Volume: 3 Pages: 69-75
• [Journal Article] Store-operated Ca^<2+> entry suppresses distention- induced ATP release from urothelium. Am J Physiol, 300, F716-720 (2011)
  • Author(s): Matsumoto-Miyai K
  • Journal Title: Am.J.Physiol.Renal Physiol Volume: 300 Issue: 3 Pages: F716-F720
  • DOI: 10.1152/ajprenal.00512.2010
  • Peer Reviewed
• [Journal Article] Pulse diode laser irradiation (830nm) of lumbosacral spinal roots diminished hyperreflexia_induced by acetic acid or PGE_2 infusion in rat urinary bladder (2011)
  • Author(s): Ito Tomoko
  • Journal Title: Luts Volume: 3 Issue: 2 Pages: 69-75
  • DOI: 10.1111/j.1757-5672.2010.00085.x
  • Peer Reviewed
• [Journal Article] Store-operated Ca^(2+) entry suppresses distention- induced ATP release from urothelium. (2011)
  • Author(s): Matsumoto-Miyai K
  • Journal Title: Am J Physiol Volume: 300
  • Peer Reviewed
• [Journal Article] Role of supraspinal and spinal α1-adrenergic receptor subtypes in micturition reflex in conscious rats (2010)
  • Author(s): Yoshizumi M, Matsumoto-Miyai K, Yonezawa A, Kawatani M
  • Journal Title: Am J Physiol Volume: 299
• [Journal Article] 排尿障害-排尿生理 (2010)
  • Author(s): 河谷正仁
  • Journal Title: 脳21 Volume: 13 Pages: 230-232
• [Journal Article] 排尿障害-膀胱上皮から分泌されるATPの排尿機能への役割 (2010)
  • Author(s): 松本-宮井和政, 河谷正仁
  • Journal Title: 脳21 Volume: 13 Pages: 247-252
• [Journal Article] カルシウムイオンによる膀胱上皮からのATP分泌の制御機構 (2010)
  • Author(s): 松本-宮井和政, 河谷正仁
  • Journal Title: 自律神経 Volume: 47 Pages: 432-435
  • NAID: 10027374791
• [Journal Article] Role of supraspinal and spinal α1-adrenergic receptor subtypes in micturition reflex in conscious rats. (2010)
  • Author(s): Yoshizumi M
  • Journal Title: Am J Physiol Volume: 299
  • Peer Reviewed
• [Journal Article] Urothelial α_<1D> receptor is predominantly involved in the adrenergic facilitation of micturition reflex (2009)
  • Author(s): Wang X, Matsumoto-Miyai K, Yoshizumi M, Ito T, Yanase H, Momota Y, Tsujimoto G, Tanoue K, Nimura T, Kawatani M
  • Journal Title: LUTS 1
• [Journal Article] Extracellular Ca^<2+> regulates the stimulus-elicited ATP release from urothelium (2009)
  • Author(s): Matsumoto-Miyai K, Kagase A, Murakawa Y, Momota Y, Kawatani M
  • Journal Title: Autonomic Neuroscience: Basic and Clinical Volume: 150 Pages: 94-99
• [Journal Article] Urothelial α_1D receptor is predominantly involved in the adrenergic facilitation of micturition reflex. (2009)
  • Author(s): Wang, X
  • Journal Title: LUTS 1
  • Peer Reviewed
• [Journal Article] Extracellular Ca^<2+> regulates the stimulus-elicited ATP release from urothelium. (2009)
  • Author(s): Matsumoto-Miyai, K
  • Journal Title: Autonomic Neuroscience : Basic and Clinica 150 Pages: 94-99
  • Peer Reviewed
• [Presentation] Regulation of distention-elicited urothelial ATP release by 5-hydroxytriptamine (2011)
  • Author(s): Kazumasa Matsumoto-Miyai
  • Organizer: Society for Neuroscience 41^<st> Annual Meeting
  • Place of Presentation: ワシントンDC、米国
  • Year and Date: 2011-11-15
• [Presentation] セロトニンによる膀胱上皮からのATP分泌の制御 (2011)
  • Author(s): 松本-宮井和政
  • Organizer: 第18回日本排尿機能学会
  • Place of Presentation: 福井市
  • Year and Date: 2011-09-16
• [Presentation] 低出力レーザーによる鎮痛発生メカニズム (2011)
  • Author(s): 河谷正仁
  • Organizer: 第23回秋田疼痛研究会
  • Place of Presentation: 秋田市
  • Year and Date: 2011-06-23
• [Presentation] 膀胱壁過伸展による膀胱上皮からのATP分泌機構 (2011)
  • Author(s): 松本-宮井和政
  • Organizer: 第23回秋田疼痛研究会
  • Place of Presentation: 秋田市
  • Year and Date: 2011-06-23
• [Presentation] セロトニンによる膀胱上皮からのATP分泌の制御 (2011)
  • Author(s): 松本-宮井和政, 善積克, 河谷正仁
  • Organizer: 第18回日本排尿機能学会
  • Place of Presentation: 福井
• [Presentation] 低出力レーザーによる鎮痛発生メカニズム (2011)
  • Author(s): 河谷正仁
  • Organizer: 第23回秋田疼痛研究会
  • Place of Presentation: 秋田
• [Presentation] 膀胱壁過伸展による膀胱上皮からのATP分泌機構 (2011)
  • Author(s): 松本-宮井和政, 善積克, 加賀瀬藍, 山田依里佳, 仁村俊枝, 畠山玲子, 河谷正仁
  • Organizer: 第23回秋田疼痛研究会
  • Place of Presentation: 秋田
• [Presentation] Regulation of distention-elicited urothelial ATP release by 5-hydroxytriptamine (2011)
  • Author(s): Matsumoto-Miyai K, Yoshizumi M, Yamada E, Kawatani M
  • Organizer: Society for Neuroscience Society for Neuroscience 41st Annual Meeting
  • Place of Presentation: Washington DC,USA
• [Presentation] The involvement of Ca^<2+> inhibitable adenylyl cyclase in the distention-elicited urothelial ATP (2010)
  • Author(s): Matsumoto-Miyai, K
  • Organizer: Society for Neuroscience 40^<th> Annual Meeting
  • Place of Presentation: San Diego, California, USA.
  • Year and Date: 2010-11-17
• [Presentation] 膀胱上皮からのATP分泌におけるCa^<2+>抑制性アデニル酸シクラーゼの役割 (2010)
  • Author(s): 松本-宮井和政
  • Organizer: 第17回日本排尿機能学会
  • Place of Presentation: 甲府市
  • Year and Date: 2010-09-30
• [Presentation] 膀胱上皮からのATP分泌の制御機構 (2010)
  • Author(s): 松本-宮井和政
  • Organizer: 第17回泌尿生殖器における信号物質の局在と役割に関
  • Place of Presentation: 秋田市
  • Year and Date: 2010-09-11
• [Presentation] 膀胱上皮からのATP分泌を制御するCa^<2+>の役割 (2010)
  • Author(s): 松本-宮井和政
  • Organizer: 第52回日本平滑筋学会総会
  • Place of Presentation: 仙台市
  • Year and Date: 2010-07-01
• [Presentation] 光の標的:炎症とTRPV1 (2010)
  • Author(s): 河谷正仁
  • Organizer: 第22回日本レーザー治療学会
  • Place of Presentation: 横浜市
  • Year and Date: 2010-06-27
• [Presentation] (2010)膀胱上皮からのATP分泌を制御するCa^<2+>の役割 (2010)
  • Author(s): 松本-宮井和政, 善積克, 河谷正仁
  • Organizer: (シンポジウム)第52回日本平滑筋学会総会
  • Place of Presentation: 仙台
• [Presentation] (2010)膀胱上皮からのATP分泌におけるCa^<2+>抑制性アデニル酸シクラーゼの役割 (2010)
  • Author(s): 松本-宮井和政, 善積克, 河谷正仁
  • Organizer: 第17回日本排尿機能学会
  • Place of Presentation: 甲府
• [Presentation] The involvement of Ca^<2+> inhibitable adenylyl cyclase in the distention-elicited urothelial ATP release (2010)
  • Author(s): Matsumoto-Miyai K, Yoshizumi M, Yamada E, Kawatani M
  • Organizer: Society for Neuroscience 40^<th> Annual Meeting
  • Place of Presentation: San Diego, California, USA
• [Presentation] Role of supraspinal and spinal α_1-adrenergic receptor subtypes in the micturition reflex in conscious rat. (2009)
  • Author(s): Yoshizumi, M
  • Organizer: International Continence Society 39^<th> Annual Meeting
  • Place of Presentation: San Francisco, USA
  • Year and Date: 2009-10-01
• [Presentation] The store-operated Ca^<2+> entry negatively regulates the distention-elicited ATP release from urothelium (2009)
  • Author(s): Matsumoto-Miyai K, Kagase A, Muraka Y, Yoshizumi M, Kawatani M
  • Organizer: International Continence Society 39^<th> Annual Meeting
  • Place of Presentation: San Francisco, USA
• [Presentation] Role of supraspinal and spinal α_1-adrenergic receptor subtypes in the micturition reflex in conscious rats (2009)
  • Author(s): Yoshizumi M, Matsumoto-Miyai K, Yonezawa, A, Kawatani M
  • Organizer: International Continence Society 39^<th> Annual Meeting
  • Place of Presentation: San Francisco, USA
• [Presentation] The Ca^<2+>-regulated mechanism of the distention-elicitted ATP release from urothelium (2009)
  • Author(s): Matsumoto-Miyai K, Yoshizumi M, Kagase A, Kawatani M
  • Organizer: Society for Neuroscience 39^<th> Annual Meeting
  • Place of Presentation: Chicago, USA
• [Presentation] Role of supraspinal and spinalα_1-adrenergic receptor subtypes in micturition reflex in conscious rats (2009)
  • Author(s): Yoshizumi M, Matsumoto-Miyai K, Yonezawa A, Kawatani M
  • Organizer: Society for Neuroscience 39^<th> Annual Meeting
  • Place of Presentation: Chicago, USA
• [Presentation] 膀胱上皮細胞からのATP分泌機構におけるCa^<2+>の関与 (2009)
  • Author(s): 松本-宮井和政, 善積克, 河谷正仁
  • Organizer: 第16回日本排尿機能学会
  • Place of Presentation: 福岡
• [Presentation] 覚醒ラットにおける排尿機能に対する中枢α_1-アドレナリン受容体サブタイプの役割 (2009)
  • Author(s): 善積克, 松本-宮井和政, 米澤章彦, 河谷正仁
  • Organizer: 第16回日本排尿機能学会
  • Place of Presentation: 福岡
• [Presentation] (2009)カルシウムイオンによる膀胱上皮細胞からのATP分泌の制御機構 (2009)
  • Author(s): 松本-宮井和政, 善積克, 加賀瀬藍, 河谷正仁
  • Organizer: 第62回日本自律神経学会
  • Place of Presentation: 和歌山