Selective labeling, imaging, and functional control of living cells

• Project/Area Number: 21651096
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Living organism molecular science
• Research Institution: Osaka University
• Principal Investigator: FUKASE Koichiz 大阪大学, 大学院・理学研究科, 教授 (80192722)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,370,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥900,000 (Direct Cost: ¥900,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,300,000)
• Keywords: 蛍光標識 / 蛍光イメージング / PETイメージング / 複合体糖鎖 / 癌細胞 / 転移 / 複合体 / 糖鎖 / 糖鎖クラスター / デンドリマー / 分子認識 / イメージング / 陽電子断層撮影

Research Abstract

New less-invasive labeling of living cells was established and applied to imaging studies based on 6π-azalectrocyclization reaction. The efficient method for the conjugation of bio-molecules was also developed using 6π-azalectrocyclization. A new antitumor molecule was developed by using template-assisted conjugation.

Research Products

• [Journal Article] Cell surface biotinylation by azaelectrocyclization and versatile approach for living cell labeling, Bioorg (2012)
  • Author(s): Tanaka, K., Yokoi, S., Morimoto, K., Iwata, T., Nakamoto, Y., Nakayama, K., Koyama, K., Fujiwara, T., Fukase, K
  • Journal Title: Med. Chem Volume: 0 Pages: 1865-1868
  • Peer Reviewed
• [Journal Article] Auxiliary-directed oxidation of ursolic acid by' Ru'-porphyrins : chemical modulation of cytotoxicity against tumor cell lines (2012)
  • Author(s): Tanaka, K., Mazumder, K., Siwu, E. R. O., Nozaki, Satoshi, Watanabe, Y., Fukase, K
  • Journal Title: Tetrahedron Lett Volume: 53 Pages: 1756-1759
  • Peer Reviewed
• [Journal Article] Auxiliary-directed oxidation of ursolic acid by 'Ru'-porphyrins : chemical modulation of cytotoxicity against tumor cell lines (2012)
  • Author(s): Tanaka, K., Mazumder, K., Siwu, E.R.O., Nozaki, Satoshi, Watanabe, Y., Fukase, K.
  • Journal Title: Tetrahedron Lett Volume: 53 Pages: 1756-1759
  • DOI: 10.1016/j.tetlet.2012.01.107
  • Peer Reviewed
• [Journal Article] Template-assisted and self-activating clicked peptide as a synthetic mimic of the SH2 domain (2012)
  • Author(s): Tanaka, K., Shirotsuki, S., Kageyama, C., Tahara, T., Nozaki, S., Siwu, E.R.O., Iwata, T., Tamura, S., Douke, S., Murakami, N., Onoe, H., Watanabe, Y., Fukase, K
  • Journal Title: ACS Chem. Biol. Volume: (未定)(in press) Pages: 637-645
  • DOI: 10.1021/cb2003175
  • Peer Reviewed
• [Journal Article] Template-assisted and self-activating clicked peptide as a synthetic mimic of the SH2 domain (2011)
  • Author(s): Tanaka, K., Shirotsuki, S., Kageyama, C., Tahara, T., Nozaki, S., Siwu, E. R. O., Iwata, T., Tamura, S., Douke, S., Murakami, N., Onoe, H., Watanabe, Y., Fukase, K
  • Journal Title: ACS Chem. Biol Volume: 7 Pages: 637-644
  • Peer Reviewed
• [Journal Article] Target-selective and fluorescence" switch-on" protein labeling by 6π-azaelectrocyclization (2011)
  • Author(s): Tanaka, K., Kitadani, M., Fukase, K
  • Journal Title: Org. Biomol. Chem Volume: 9 Pages: 5346-5349
  • Peer Reviewed
• [Journal Article] Exploring a unique reactivity of 6π-azaelectrocyclization to enzyme inhib ition, natural products synthesis, and molecular imaging : an approach to chemical biology by synthetic chemis ts (2011)
  • Author(s): Tanaka, K., Fukase, K., Katsumura, S
  • Journal Title: Synlett Volume: 15 Pages: 2115-2139
  • Peer Reviewed
• [Journal Article] Heterogeneity Effects of Glycan Clusters on Microarrays and Dendrimers (2011)
  • Author(s): K.Tanaka
  • Journal Title: TIGG Volume: 23 Issue: 131 Pages: 155-157
  • DOI: 10.4052/tigg.23.155
  • NAID: 10029583041
  • ISSN: 0915-7352
  • Peer Reviewed
• [Journal Article] Target-Selective and Fluorescence "Switch-On" Protein Labeling by 6y 6π-Azaelectro cyclization (2011)
  • Author(s): Tanaka, K., Kitadani, M., Fukase, K.
  • Journal Title: Org.Biomol.Chem. Volume: 9 Pages: 5346-5349
  • DOI: 10.1039/c1ob05320e
  • Peer Reviewed
• [Journal Article] Exploring A Unique Reactivity of 6π-Azaelectrocyclization to Enzyme Inhibition, Natural Products Synthesis, and Molecular Imaging : An Approach to Chemical Biology by Synthetic Chemists (2011)
  • Author(s): Tanaka, K., Fukase, K., Katsumura, S.
  • Journal Title: SYNLETT Volume: 15 Pages: 2115-2139
  • DOI: 10.1055/s-0030-1261192
  • Peer Reviewed
• [Journal Article] Asymmetric synthesis of^<14> C-labeled L-Proparagylglycine (2010)
  • Author(s): Kurosawa, M., Tanaka, K., and Fukase, K
  • Journal Title: Radioisotopes Volume: 59(12) Pages: 721-726
  • Peer Reviewed
• [Journal Article] Non-invasive imaging of dendrimer-type N-glycan clusters : in vivo dynamics dependence on oligosaccharide structure, Chen, C. W., Paulson, C. J., Watanabe, Y., Fukase, K (2010)
  • Author(s): Tanaka, K., Siwu, R. O. E., Minami, K., Hasegawa, K., Nozaki, S., Kanayama, Y., Koyama, K
  • Journal Title: Angew. Chem. Int. Ed. Volume: 49 Pages: 8195-8200
  • Peer Reviewed
• [Journal Article] New strategy in synthetic biology : from enzyme inhibition and natural products synthesis to PET imaging by 6π-azaelectrocyclization (2010)
  • Author(s): Tanaka, K., Fukase, K., Katsumura, S.
  • Journal Title: The Chemical Record Volume: 10 Pages: 119-139
  • Peer Reviewed
• [Journal Article] A combined 6π-azaelectrocyclization/ staudinger approach to protein & cell engineering : non-invasive tumor targeting by N-Glycan-engineered lymphocytes (2010)
  • Author(s): Tanaka, K., Minami, K., Tahara, T., Siwu, E. R. O., Koyama, K., Nozaki, S., Onoe, H., Watanabe, Y., Fukase, K.
  • Journal Title: J. Carbohydr. Chem. Volume: 29 Pages: 118-132
  • Peer Reviewed
• [Journal Article] Electrocyclization-based labeling allows efficient in Vivo imaging of cellular trafficking (2010)
  • Author(s): Tanaka, K., Minami, K., Tahara, T., Fujii, Y., Siwu, E. R. O., Nozaki, S.; Onoe, H., Yokoi, S., Koyama, K., Watanabe, Y., Fukase, K.
  • Journal Title: ChemMedChem Pages: 841-845
  • Peer Reviewed
• [Journal Article] Asymmetric synthesis of ^<14>C-labeled L-Proparagylglycine (2010)
  • Author(s): Kurosawa, M., Fukase, K., et al.
  • Journal Title: Radioisotopes Volume: 59(12) Pages: 17-22
  • Peer Reviewed
• [Journal Article] Noninvasive imaging of dendrimer-type N-glycan clusters : in vivo dynamics dependence on oligosaccharide structure. (2010)
  • Author(s): Tanaka, K., Fukase, K., et al.
  • Journal Title: Angew.Chem.Int.Ed. Volume: 49 Pages: 8195-8200
  • Peer Reviewed
• [Journal Article] New strategy in synthetic biology : from enzyme inhibition and natural products synthesis to PET imaging by 6II-azaelectrocyclization (2010)
  • Author(s): Tanaka, K., Fukase, K., et al.
  • Journal Title: The Chemical Record Volume: 10 Pages: 119-139
  • Peer Reviewed
• [Journal Article] A combined 6p-azaelectrocyclization/staudinger approach to protein & cell engineering : non-invasive tumor targeting by N-Glycan-engineered lymphocytes (2010)
  • Author(s): Tanaka, K., Fukase, K., et al.
  • Journal Title: J.Carbohydr.Chem. Volume: 29 Pages: 118-132
  • Peer Reviewed
• [Journal Article] Electrocyclization-based labeling allows efficient in Vivo imaging of cellular trafficking (2010)
  • Author(s): Tanaka, K., Fukase, K., et al.
  • Journal Title: ChemMedChem Volume: 5 Pages: 841-845
  • Peer Reviewed
• [Journal Article] Library-directed Solution-and Solid-phase Synthesis of 2, 4-Disubstituted Pyridines : One-pot Approach through 6. pi.-Azaelectrocyclization (2009)
  • Author(s): Sakaguchi, T., Kobayashi, T., Hatano, S., Tsuchikawa, H., Fukase, K., Tanaka, K., and Katsumura, S.
  • Journal Title: Chemistry-An Asian Journal Volume: 4(10) Pages: 1573-1577
  • Peer Reviewed
• [Journal Article] Library-directed Solution- and Solid-phase Synthesis of 2, 4-Disubstituted Pyridines : One-pot Approach through 6.pi.-Azaelectrocyclization (2009)
  • Author(s): Sakaguchi, T.
  • Journal Title: Chemistry-An Asian Journal 4(10) Pages: 1573-1577
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): 田中克典, 深瀬浩一
  • Journal Title: 複合糖質の化学と最新応用技術(鎖のインビボバイオイメージング) Pages: 235-244
• [Journal Article] (2009)
  • Author(s): 田中克典, 深瀬浩一
  • Journal Title: 第三の生命鎖「糖鎖」を活用・制御するケミカルバイオロジー,月刊BIO INDUSTRY(シーエムシー出版.) Pages: 29-36
• [Presentation] 固相上での閉環メタセシス/鋳型誘起反応を駆使した癌ターゲティングペプチドの創成 (2012)
  • Author(s): 岩田隆幸、田原強、野崎聡、渡辺恭良、田中克典、深瀬浩一
  • Organizer: 日本化学会第92春季年会(2012)
  • Place of Presentation: 慶應義塾大学(神奈川県)
• [Presentation] 固相上での閉環メタセシス/鋳型誘起反応を駆使した癌ターゲティングペプチドの創成 (2012)
  • Author(s): 岩田隆幸、田原強、野崎聡、渡辺恭良、田中克典、深瀬浩一
  • Organizer: 日本化学会第92春季年会
  • Place of Presentation: 神奈川県、慶應義塾大学
• [Presentation] リン酸化タンパク質認識ペプチドの創製とその抗腫瘍作用 (2011)
  • Author(s): 深瀬浩一
  • Organizer: 第14回ペプチドフォーラム
  • Place of Presentation: 鹿児島大学理学部
  • Year and Date: 2011-12-16
• [Presentation] 固相上での閉環メタセシスとHuisgen環化反応を経た人工抗癌ペプチドの効率合成と評価 (2011)
  • Author(s): 岩田隆幸、田原強、野崎聡、渡辺恭良、田中克典、深瀬浩一
  • Organizer: 第31回有機合成若手セミナー「明日の有機合成を担う人のために」
  • Place of Presentation: 京都大学吉田キャンパス
  • Year and Date: 2011-11-16
• [Presentation] 固相上での閉環メタセシスとHuisgen環化反応を経た人工抗癌ペプチドの効率合成と評価 (2011)
  • Author(s): 岩田隆幸、田原強、野崎聡、渡辺恭良、田中克典、深瀬浩一
  • Organizer: 回有機合成若手セミナー「明日の有機合成を担う人のために」
  • Place of Presentation: 京都大学
  • Year and Date: 2011-11-16
• [Presentation] 有機合成と生体イメージングを用いて糖鎖の生物機能を解明する (2011)
  • Author(s): 深瀬浩一
  • Organizer: 第4回生体分子科学シンポジウム(第2回ケミカルバイオロジー研究所シンポジウム)
  • Place of Presentation: 大阪府立大学理学系研究科
  • Year and Date: 2011-03-04
• [Presentation] 有機合成と生体イメージングを用いて糖鎖の生物機能を解明する (2011)
  • Author(s): 深瀬浩一
  • Organizer: 第4回生体分子科学シンポジウム
  • Place of Presentation: 大阪府立大学理学系研究科(招待講演)
  • Year and Date: 2011-03-04
• [Presentation] Glyco-imag ing for glycan dynamics study (2011)
  • Author(s): K. Fukase
  • Organizer: The 71st Okazaki Conference" New perspectives o n molecular science of glycoconjugates"
  • Place of Presentation: Osakazaki Conference Center, NINS, Japan
• [Presentation] 標的リジンとの電子環状反応に起因するドミノ反応型蛍光回復標識法 (2011)
  • Author(s): 北谷方嵩、田中克典、深瀬浩一
  • Organizer: 第5回バイオ関連化学シンポジウム
  • Place of Presentation: つくば国際会議場「エポカルつくば」
• [Presentation] 鋳型誘起合成によるGrb2-SH2ドメインイディオタイプの創製と生体内シグナルの制御 (2011)
  • Author(s): 岩田隆幸, 白坏早苗, 景山知佳, 田原強, 野崎聡, 渡辺恭良, 田中克典, 深瀬浩一
  • Organizer: 天然物化学談話会
  • Place of Presentation: 熱川ハイツ(静岡県)
• [Presentation] 細胞のグライコイメージング (2011)
  • Author(s): 深瀬浩一
  • Organizer: 第6回日本分子イメージング学会
  • Place of Presentation: 神戸国際会議場、糖タンパク質、糖鎖クラスター
• [Presentation] New functional analysis of glycans based on chemical synthesis and bio-imaging (2011)
  • Author(s): Koichi Fukase
  • Organizer: Frontiers of Chemical Biology at Peking University Shenzhen Graduate School, the School of Chemical B iology and Biotechnology
  • Place of Presentation: China
• [Presentation] 標的指向型ライブラリー構築と生命科学への貢献 (2011)
  • Author(s): 深瀬浩一
  • Organizer: 日本化学会第91回春季年会
  • Place of Presentation: 神奈川大学
• [Presentation] Glyco-imaging for glycan dynamics study (2011)
  • Author(s): K.Fukase
  • Organizer: The 71st Okazaki Conference "New perspectives on molecular science of glycoconjugates"
  • Place of Presentation: 愛知県、Osakazaki Conference Center(招待講演)
• [Presentation] 鋳型誘起合成によるGrb2-SH2ドメインイディオタイプの創製と生体内シグナルの制御 (2011)
  • Author(s): 岩田隆幸, 白坏早苗, 景山知佳, 田原強, 野崎聡, 渡辺恭良, 田中克典, 深瀬浩一
  • Organizer: 第46回天然物化学談話会
  • Place of Presentation: 静岡県、熱川ハイツ
• [Presentation] 糖タンパク質、糖鎖クラスター、細胞のグライコイメージング (2011)
  • Author(s): 深瀬浩一
  • Organizer: 第6回日本分子イメージング学会
  • Place of Presentation: 神戸国際会議場(招待講演)
• [Presentation] New functional analysis of glycans based on chemical synthesis and bio-imaging (2011)
  • Author(s): Koichi Fukase
  • Organizer: Frontiers of Chemical Biology
  • Place of Presentation: Peking University, China
• [Presentation] Isolation and Cytotoxicity of Ursolic Acid Derivatives from Bangladeshi Medicinal Plants, Saurauja roxburghii (2011)
  • Author(s): M.Kishor, K.Fukase
  • Organizer: 日本化学会第91回春季年会
  • Place of Presentation: 神奈川大学
• [Presentation] Synthesis of Polylysine-Based N-Glycans Dendrimers as Noninvasive Imaging Tracers (2010)
  • Author(s): Katsunori Tanaka, Eric R. O. Siwu, Tohru Watanabe, and Koichi Fukase
  • Organizer: 5^<th> International Peptide Symposium
  • Place of Presentation: Kyoto International Conference Center
  • Year and Date: 2010-12-06
• [Presentation] Self-Activating" & Induced-Fit" Approach to Peptidyl Mimic of Grb2-SH2 Domain (2010)
  • Author(s): K. Tanaka, T. Iwata, S. Shirotsuki, C. Kageyama, T. Tahara, S Nozaki, E. R. O. Siwu, S. Tamura, S. Douke, N. Murakami, H. Onoe, Y. Watanabe, K. Fukase
  • Organizer: 5^<th> International Peptide Symposium
  • Place of Presentation: Kyoto International Conference Center
  • Year and Date: 2010-12-06
• [Presentation] Synthesis of Polylysine-Based N-Glycan Dendrimers as Noninvasive Imaging Tracers (2010)
  • Author(s): K., Tanaka, K.Fukase
  • Organizer: 5^<th> International Peptide Symposium
  • Place of Presentation: 国立京都国際会館(京都府)
  • Year and Date: 2010-12-06
• [Presentation] 非侵襲的イメージングを用いたタンパク質、細胞、およびデンドリマー上のN-結合型糖鎖の機能解析-N-結合型糖鎖による非侵襲的イメージングとターゲティング (2010)
  • Author(s): 田中克典・深瀬浩一
  • Organizer: 第59回高分子学会
  • Place of Presentation: 北海道大学
  • Year and Date: 2010-09-15
• [Presentation] 非侵襲的イメージングを用いたタンパク質、細胞、およびデンドリマー上のN-結合型糖鎖の機能解析-N-結合型糖鎖による非侵襲的イメージングとターゲティング- (2010)
  • Author(s): 田中克典、深瀬浩一
  • Organizer: 第59回高分子学会
  • Place of Presentation: 北海道大学
  • Year and Date: 2010-09-15
• [Presentation] Azaelectrocyclization-Based Labeling & Engineering Allow Non-Invasive Imaging of Cellular Trafficking and Tumor Targeting by Lymphocytes (2010)
  • Author(s): Katsunori Tanaka, Kaori Minami, Tsuyoshi Tahara, Yohei Fujii, Eric, R. O. Siwu, Satoshi Nozaki, Hirotaka Onoe, Satomi Yokoi, Koichi Koyama, Yasuyoshi Watanabe, and Koichi Fukase
  • Organizer: World Molecular Imaging Congress(WMIC)
  • Place of Presentation: Kyoto International Conference Center
  • Year and Date: 2010-09-09
• [Presentation] Azaelectrocyclization-Based Labeling & Engineering Allow Non-Invasive Imaging of Cellular Trafficking and Tumor Targeting by Lymphocytes (2010)
  • Author(s): K.Tanaka, K.Fukase
  • Organizer: World Molecular Imaging Congress (WMIC)
  • Place of Presentation: 京都国際会館(京都府)
  • Year and Date: 2010-09-09
• [Presentation] 基質隣接効果による初めての"head-to-head"イミン二量化反応:縮環1,2-ジアゼチジンの直接的[2+2]合成 (2010)
  • Author(s): 広崎真司、深瀬浩一
  • Organizer: 第37回有機反応懇談会
  • Place of Presentation: 京都大学宇治キャンパス
  • Year and Date: 2010-08-27
• [Presentation] リン酸化タンパク質を鋳型とした選択的ペプチド連結反応の開発と細胞内シグナル伝達を制御する機能性分子創成への応用 (2010)
  • Author(s): 岩田隆幸、白坏早苗、景山知佳、田原強、野崎聡、渡辺恭良、田中克典、深瀬浩一
  • Organizer: 第37回有機反応懇談会
  • Place of Presentation: 京都大学宇治キャンパスおうばくプラザきはだホール
  • Year and Date: 2010-08-02
• [Presentation] リン酸化タンパク質を鋳型とした選択的ペプチド連結反応の開発と細胞内シグナル伝達を制御する機能性分子創成への応用 (2010)
  • Author(s): 岩田隆幸、深瀬浩一
  • Organizer: 第37回有機反応懇談会
  • Place of Presentation: 京都大学宇治キャンパス
  • Year and Date: 2010-08-02
• [Presentation] Azaelectrocyclization-Based Labelling and Bioconjugation of Proteins and Living Cells : Application to Imaging of N-Glycans and Lymphocyte (2010)
  • Author(s): Koichi Fukase
  • Organizer: The 4th HiPep-Okinawa International Workshp
  • Place of Presentation: Okinawa Industry Support Center
  • Year and Date: 2010-07-09
• [Presentation] Azaelectrocyclization-Based Labelling and Bioconjugation of Proteins and Living Cells : Application to Imaging of N-Glycans and Lymphocytes (2010)
  • Author(s): K.Fukase
  • Organizer: The 4th HiPep-Okinawa International Workshop
  • Place of Presentation: Okinawa Industry Support Center(招待講演)
  • Year and Date: 2010-07-09
• [Presentation] in vivo生体分子科学を目指した生物活性プローブの創成:糖鎖のPETイメージングプローブとSH2ドメインミミック (2010)
  • Author(s): 深瀬浩一
  • Organizer: 第16回数理医学研究会
  • Place of Presentation: 大阪大学基礎工学研究科
  • Year and Date: 2010-03-05
• [Presentation] in vivo生体分子科学を目指した生物活性プローブの創成:糖鎖のPETイメー ジングプローブとSH2ドメインミミック (2010)
  • Author(s): 深瀬浩一
  • Organizer: 第16回数理医学研究会
  • Place of Presentation: 近畿大学(大阪)
  • Year and Date: 2010-03-05
• [Presentation] タンパク質、糖鎖の迅速標識法とPETへの応用 (2010)
  • Author(s): 深瀬浩一
  • Organizer: 保健医療分野における基礎研究推進事業「TGF-β活性化反応を標的とした肝疾患の新規診断法、治療・予防法の開発」研究班シンポジウム
  • Place of Presentation: 千里ライフサイエンスセンター(大阪)
  • Year and Date: 2010-02-20
• [Presentation] タンパク質、糖鎖の迅速標識法とPETへの応用 (2010)
  • Author(s): 深瀬浩一
  • Organizer: 保健医療分野における基礎研究推進事業「TGFβ活性化反応を標的とした肝疾患の新規診断法、治療・予防法の開発」研究班シンポジウム
  • Place of Presentation: 千里ライフサイェンスセンター(大阪)
  • Year and Date: 2010-02-20
• [Presentation] Synthetic approach for in vivo functional studies of glycans : Applica tion of PET imaging (2010)
  • Author(s): Koichi Fukase
  • Organizer: The 2010 International Chemical Congress of Pacific Basin Societies(Pacifichem 2010)
  • Place of Presentation: Honolulu, USA
• [Presentation] Azaelectrocyclization-Based Labeling and Engineering of Proteins & Living Cells : Application to Non-invasive Imaging of N-Glycans (2010)
  • Author(s): K. Tanaka and K. Fukase
  • Organizer: The 2010 International Chemical Congress of Pacific Basin Societies(Pacifichem 2010)
  • Place of Presentation: Honolulu, USA
• [Presentation] Synthetic Approach for Elucidating Glycan Codes (2010)
  • Author(s): Koichi Fukase, Yukari Fujimoto, Katsunori Tanaka
  • Organizer: The 5th International Conference on Cutting-Edge Organic Chemistry in Asia(ICCEOCA-5)/ The 1st New phase International Conference on Cutting-Edge Organic Chemistry in Asia(NICCEOCA-1)
  • Place of Presentation: Ambassador Hotel at Hsinchu, Taiwan
• [Presentation] Synthetic Approach for Elucidation of Biological Relevance of Glycans (2010)
  • Author(s): Koichi Fukase
  • Organizer: The Sixth International Symposium on Integrated Synthesis(ISIS-6)
  • Place of Presentation: Seaside Hotel Maiko Villa Kobe
• [Presentation] Synthesis of Polylysine-Based Glycodendrimer Through Self-Activating Click Reaction、Tohru Watanabe, Eric Richard Okitavianus (2010)
  • Author(s): Yuka Nakamoto, Katsunori Tanaka, Koichi Fukase
  • Organizer: The Sixth International Symposiu m on Integrated Synthesis(ISIS-6)
  • Place of Presentation: Seaside Hotel Maiko Villa Kobe
• [Presentation] アザ電子環状反応を基盤とするタンパク質および細胞表層の高速標識化とN-結合型糖鎖の"生きている動物内"でのイメージング (2010)
  • Author(s): 田中克典、南香莉、Eric R. O. Siwu、増山達郎、小山幸一、横井里美、長谷川功紀、田原強、水間広、野崎聡、金山洋介、和田康弘、尾上浩隆、渡辺恭良、深瀬浩一
  • Organizer: 第52回天然有機化合物討論会
  • Place of Presentation: グランシップ(静岡)
• [Presentation] 高速6π-アザ電子環状反応による高速アミノ基標識キット、STELLA+の開発 (2010)
  • Author(s): 田中克典、横井里美、中山郁理、小山幸一、長谷川功紀、田原強、渡辺恭良、深瀬浩一
  • Organizer: 第4回バイオ関連化学シンポジウム
  • Place of Presentation: 大阪大学
• [Presentation] Noninvasive PET & Fluorescence Imaging of N-Glycans : Sialic Acid-Dependent In Vivo Dynamics of Proteins, Glycoclusters and Living Cell s (2010)
  • Author(s): Katsunori Tanaka and Koichi Fukase
  • Organizer: Sialoglyco 2010
  • Place of Presentation: Potsdam, Germany
• [Presentation] Synthetic Approach toward Understanding In Vivo Functions of Glycans (2010)
  • Author(s): Koichi Fukase
  • Organizer: The 25th International Carbohydrate Symposium(ICS2010)
  • Place of Presentation: Makuhari Messe(Chiba)
• [Presentation] Synthetic Study and Biological Investigation of New N-Glycan in、Mouse Brain (2010)
  • Author(s): G. M. Bao, K. Tanaka, K. Ikenaka, K. Fukas e
  • Organizer: The 25th International Carbohydrate Symposium(ICS2010)
  • Place of Presentation: Makuhari Messe(Chiba)
• [Presentation] Chemistry-based Protein & Cell-Surface Engineering by N-Glycans (2010)
  • Author(s): K. Tanaka, K. Minami, E. R. Siwu, K. Fukase
  • Organizer: The 25th International Carbohydrate Symposium(ICS2010)
  • Place of Presentation: Makuhari Messe(Chiba)
• [Presentation] Remarkable Structural Dependency of N-Glycan Clusters on In、Vivo Dynamics (2010)
  • Author(s): E. R. Siwu, K. Tanaka, K. Minami, K. Hasegaw a, S. Nozaki, Y. Kanayama, K. Koyama, W. C. Chen, J. Paulson, Y. Watanabe, K. Fukase
  • Organizer: The 25th International Carbohydrate Symposium(ICS2010)
  • Place of Presentation: Makuhari Messe(Chiba)
• [Presentation] 糖鎖機能研究会・・・分子レベルでの解明を目指して、イメージングを利用した糖鎖の新しい機能探索 (2010)
  • Author(s): 深瀬浩一、田中克典、Eric Siwu、南香莉、渡辺恭良、長谷川功紀、野崎聡、金山洋介、小山幸一、三善英知、中川勉、里村慎二
  • Organizer: 平成22年度生理学研究所研究会
  • Place of Presentation: 生理学研究所(愛知県)
• [Presentation] New Synthetic Approach for Phosphopeptide Recognition Module That Shows Antitumor Action to Specific Cancer (2010)
  • Author(s): Koichi Fukase, Katsunori Tanaka, Sanae Shir otsuki, Chika Kageyama, Takayuki Iwata, Tsuyoshi Tahara, Satoshi Nozaki, Akiyoshi Watanabe
  • Organizer: 1st Asian Chemical Biology Conference
  • Place of Presentation: Seoul National University, Seoul, Korea
• [Presentation] Induced-fit型クリックペプチドを用いたSH2ドメインイディオタイプ創製への新規合成化学的アプローチ (2010)
  • Author(s): 田中克典, 白坏早苗, 景山知佳, 田原強, 野崎聡, 渡辺恭良, 深瀬浩一
  • Organizer: 日本ケミカルバイオロジー学会第5回年会
  • Place of Presentation: 慶応大学〔神奈川県)
• [Presentation] Synthetic Approach for In Vivo Functional Studies of Glycans (2010)
  • Author(s): Koichi Fukase
  • Organizer: The 21st French-Japanese Symposium on Medicinal and Fine Chemistry(FJS2010)
  • Place of Presentation: Kyoto Garden Palace
• [Presentation] Unprecedented[ 2+ 2]-Condensation of Imines : Simulataneous C-C & N-N Bond Formations to 1, 2-Diazetidenes (2010)
  • Author(s): Katsunori Tanaka, Shinji Hirosaki, Takayuki Iwata, Eric, R. O. Siwu, Koichi Fukase
  • Organizer: The 21st French-Japanese Symposium on Medicinal and Fine Chemistry(FJS2010)
  • Place of Presentation: Kyoto Garden Palace
• [Presentation] Non-Invasive In Vivo Imaging of N-Glycans (2010)
  • Author(s): Katsunori Tanaka and Koic hi Fukase
  • Organizer: International Symposium on Organelle Networks : Interface among Infection-Immunity
  • Place of Presentation: Cell Biology and Glycobio logy, Senri Life Science Center(Osaka)
• [Presentation] 6π-電子環状反応を用いたタンパク質への糖鎖エンジニアリング、南香莉、SIWU, Eric R. O. (2010)
  • Author(s): 田中克典、深瀬浩一
  • Organizer: 日本化学会第90回春季年会
  • Place of Presentation: 近畿大学(大阪)
• [Presentation] イメージングを活用した糖鎖のin vivo生体分子科学 (2010)
  • Author(s): 深瀬浩一
  • Organizer: 第12回生命化学研究会
  • Place of Presentation: 芦原温泉清風荘(福井)
• [Presentation] In Vivo Imaging of N-Glycan Dendrimers by Fluorescence and Positron Emission Tomography(PET) (2010)
  • Author(s): Katsunori Tanaka, Eric R. O. Siwu, Kaori Minami, Koki Hasegawa, Satoshi Nozaki, Yousuke Kanayama, Yasuyoshi Watanabe, Koichi Koyama, Koichi Fukase
  • Organizer: Combinatorial Chemistry and Chemical Biology toward A New Paradigm for Drug Discovery(CCCB)
  • Place of Presentation: Senri Life Sc ience Center(Osaka)
• [Presentation] Synthetic approach for in vivo functional studies of glycans : Application of PET imaging (2010)
  • Author(s): K.Fukase
  • Organizer: The 2010 International Chemical Congress of Pacific Basin Societies (Pacifichem 2010)
  • Place of Presentation: Honolulu, USA(招待講演)
• [Presentation] Unprecedented [2+2]-Condensation of Imines : Remarkable Substituent Effects and Synthetic Applications (2010)
  • Author(s): K.Tanaka, K.Fukase
  • Organizer: The 2010 International Chemical Congress of Pacific Basin Societies (Pacifichem 2010)
  • Place of Presentation: Honolulu, USA
• [Presentation] Synthetic Approach for Elucidating Glycan Codes (2010)
  • Author(s): K.Fukase
  • Organizer: The 5th International Conference on Cutting-Edge Organic Chemistry in Asia (ICCEOCA-5)/The 1st New phase International Conference on Cutting-Edge Organic Chemistry in Asia (NICCEOCA-1)
  • Place of Presentation: Hsinchu, Taiwan(招待講演)
• [Presentation] Synthetic Approach for Elucidation of Biological Relevance of Glycans (2010)
  • Author(s): K.Fukase
  • Organizer: The Sixth International Symposium on Integrated Synthesis (ISIS-6)
  • Place of Presentation: Seaside Hotel Maiko Villa Kobe(招待講演)
• [Presentation] Synthesis of Polylysine-Based Glycodendrimer Through Self-Activating Click Reaction (2010)
  • Author(s): T.Watanabe, K.Fukase
  • Organizer: The Sixth International Symposium on Integrated Synthesis (ISIS-6)
  • Place of Presentation: Seaside Hotel Maiko Villa Kobe
• [Presentation] 共役イミン誘導体の初めての[2+2]反応によるワンポット1,2-ジアゼチジン合成 (2010)
  • Author(s): 田中克典、深瀬浩一
  • Organizer: 第40回複素環化学討論会
  • Place of Presentation: 東北大学(宮城県)
• [Presentation] アザ電子環状反応を基盤とするタンパク質および細胞表層の高速標識化とN-結合型糖鎖の"生きている動物内"でのイメージング (2010)
  • Author(s): 田中克典、深瀬浩一
  • Organizer: 第52回天然有機化合物討論会
  • Place of Presentation: グランシップ(静岡県)
• [Presentation] Noninvasive PET & Fluorescence Imaging of N-Glycans : Sialic Acid-Dependent In Vivo Dynamics of Proteins, Glycoclusters and Living Cells (2010)
  • Author(s): K.Tanaka, K.Fukase
  • Organizer: Sialoglyco 2010
  • Place of Presentation: Potsdam, Germany
• [Presentation] Synthetic Approach toward Understanding In Vivo Functions of Glycans (2010)
  • Author(s): K.Fukase
  • Organizer: The 25th International Carbohydrate Symposium (ICS 2010)
  • Place of Presentation: 幕張メッセ(千葉県)(招待講演)
• [Presentation] Synthetic Study and Biological Investigation of New N-Glycan in Mouse Brain (2010)
  • Author(s): G.M.Bao, K.Fukase
  • Organizer: The 25th International Carbohydrate Symposium (ICS 2010)
  • Place of Presentation: 幕張メッセ(千葉県)
• [Presentation] Chemistry-based Protein & Cell-Surface Engineering by N-Glycans (2010)
  • Author(s): K.Tanaka, K.Fukase
  • Organizer: The 25th International Carbohydrate Symposium (ICS 2010)
  • Place of Presentation: 幕張メッセ(千葉県)
• [Presentation] Remarkable Structural Dependency of N-Glycan Clusters on In Vivo Dynamics (2010)
  • Author(s): E.R.Siwu, K.Fukase
  • Organizer: The 25th International Carbohydrate Symposium (ICS 2010)
  • Place of Presentation: 幕張メッセ(千葉県)
• [Presentation] イメージングを利用した糖鎖の新しい機能探索 (2010)
  • Author(s): 深瀬浩一
  • Organizer: 平成22年度生理学研究所研究会
  • Place of Presentation: 生理学研究所(愛知県)(招待講演)
• [Presentation] Synthesis of new probes for chemical glycobiology : application to PET and fluorescent imaging of glycoproteins, glycoclusters, and living cells (2010)
  • Author(s): K.Fukase
  • Organizer: 1st Asian Chemical Biology Conference
  • Place of Presentation: Seoul, Korea
• [Presentation] Synthetic Approach for In Vivo Functional Studies of Glycans (2010)
  • Author(s): K.Fukase
  • Organizer: The 21st French-Japanese Symposium on Medicinal and Fine Chemistry (FJS2010)
  • Place of Presentation: Kyoto Garden Palace(招待講演)
• [Presentation] Unprecedented [2+2]-Condensation of Imines : Simulataneous C-C & N-N Bond Formations to 1,2-Diazetidenes (2010)
  • Author(s): K.Tanaka, K.Fukase
  • Organizer: The 21st French-Japanese Symposium on Medicinal and Fine Chemistry (FJS2010)
  • Place of Presentation: Kyoto Garden Palace
• [Presentation] Induced-fit型クリックペプチドを用いたSH2ドメインイディオタイプ創製への新規合成化学的アプローチ (2010)
  • Author(s): 田中克典、深瀬浩一
  • Organizer: 日本ケミカルバイオロジー学会 第5回年会
  • Place of Presentation: 慶応大学 日吉キャンパス(神奈川県)
• [Presentation] Non-Invasive In Vivo Imaging of N-Glycans (2010)
  • Author(s): K.Tanaka, K.Fukase
  • Organizer: International Symposium on Organelle Networks : Interface among Infection-Immunity, Cell Biology and Glycobiology
  • Place of Presentation: 千里ライフサイエンスセンター(大阪)
• [Presentation] 6II-電子環状反応を用いたタンパク質への糖鎖エンジニアリング (2010)
  • Author(s): 南香莉
  • Organizer: 日本化学会第90回春季年会
  • Place of Presentation: 近畿大学(大阪)
• [Presentation] イミン誘導体の形式的[2+2]反応と合成的展開 (2010)
  • Author(s): 田中克典
  • Organizer: 日本化学会第90回春季年会
  • Place of Presentation: 近畿大学(大阪)
• [Presentation] 有機合成で生物機能に迫る:マイクロフロー合成と固相合成による糖鎖構築と糖鎖の新機能解析 (2009)
  • Author(s): 深瀬浩一
  • Organizer: 第34回有機反応懇談会
  • Place of Presentation: 関西学院大学
  • Year and Date: 2009-08-03
• [Presentation] タンパク質、および細胞上での革新的有機合成反応の開発:6p-アザ電子環状反応を用いたシンセティックバイオロジー (2009)
  • Author(s): Eric R.O.Siwu
  • Organizer: 第34回有機反応懇談会
  • Place of Presentation: 関西学院大学、三田(兵庫)
  • Year and Date: 2009-08-03
• [Presentation] Nanomolar-Scale Lysine Labeling by 6p-Azaelectrocyclization : Biomolecule-and Whole Cell-Based In Vivo Imaging and Tumor Targeting (2009)
  • Author(s): K. Tanaka, K. Minami, E. R. O. Siwu, T. Tahara, S. Nozaki, Y. Watanabe, K. Koyama, S. Yokoi, and K. Fukase
  • Organizer: Combinatorial Chemistry and Chemical Biology toward A New Paradigm for Drug Discovery(CCCB)
  • Place of Presentation: Senr i Life Science Center(Osaka)
• [Presentation] In vivo imaging of glycoproteins and glycoclusters (2009)
  • Author(s): Koichi Fukase, Katsunori Tanaka, Tatsuro Masuyama, Kaori Minami, Eric R. O. Siwu, Koki Hasegawa, Tsuyoshi Tahara, Hiroshi Mizuma, Yasuhiro Wada, Yasuyoshi Watanabe
  • Organizer: 2nd Switzerland. Japan Biomolecular Chemistry Symposium
  • Place of Presentation: Tokyo Univ.
• [Presentation] 有機合成で生物機能に迫る:マイクロフロー合成と固相合成による糖鎖構築と糖鎖の新機能解析 (2009)
  • Author(s): 深瀬浩一
  • Organizer: 第29回日本糖質学会年会
  • Place of Presentation: 世界生活文化センター(高山)
• [Presentation] Molecular Probe Method for Studying the Biofunctional Role of Glycoconjugates (2009)
  • Author(s): Koichi Fukase
  • Organizer: The 25th Naito Conference
  • Place of Presentation: Chemical Biology II-An Emerging Filed Inspir ed by Natural Product Chemistry-, Sapporo
• [Presentation] In Vivo Imaging of N-Glycan Dendrimers by Fluorescence and Positron Emission Tomography(PET) (2009)
  • Author(s): Eric R. O. Siwu, K. Minami, K. Hasegawa, S. Nozaki, Y. Kanayama, K. Koyama, Y. Watanabe, K. Tanaka, K. Fukase
  • Organizer: The 25th Naito Conference
  • Place of Presentation: Chemical Biology II-An Emerging Filed Inspir ed by Natural Product Chemistry-、Sapporo
• [Presentation] Fast Labeling of Lysines via 6π-Azaelectrocyclization : Application to Pet Imaging of Glycoproteins and Glycociusters (2009)
  • Author(s): K. Fukase, K. Tanaka, T. Masuyama, K. Minami, E. R. O. Siwu, K. Hasegawa, T. Tahara, H. Mizuma, Y. Wada, Y. Watanabe
  • Organizer: 15th European Carbohydrate Symposium(eurocarb15)
  • Place of Presentation: Vienna, Austria
• [Presentation] 糖鎖の合成とin vivo生体分子科学 (2009)
  • Author(s): 深瀬浩一
  • Organizer: 第44回天然物化学談話会
  • Place of Presentation: つくばグランドホテル
• [Presentation] Chemical Glycobiology of Self/ Non-self Recognition (2009)
  • Author(s): K. Fukase
  • Organizer: Gordon Research Conferences
  • Place of Presentation: Carbohydrates, Tilton School, Tilton, NH
• [Presentation] アスパラギン結合型糖タンパク質糖鎖クラスターの蛍光およびPETイメージング (2009)
  • Author(s): Eric R. O. Siwu1、南香莉、長谷川功紀、野崎聡、金山洋介、渡辺恭良、小山幸一、田中克典、深瀬浩一
  • Organizer: 第4回年会、神戸市産業振興センター
  • Place of Presentation: 日本ケミカルバイオロジー学会
• [Presentation] ポスタータイトル:高速6π-アザ電子環状反応を用いた細胞表層への化学的標識と糖鎖エンジニアリング:細胞のインビボイメージング (2009)
  • Author(s): 南香莉、Eric. R. O. Siwu、田原強、野崎聡、渡辺恭良、小山幸一、田中克典、深瀬浩一
  • Organizer: 第4回年会、神戸市産業振興センター
  • Place of Presentation: 日本ケミカルバイオロジー学会
• [Presentation] Nanomolar-Scale Lysine Labeling by 6□-Azaelectrocyclization : Biomolecule- and Whole Cell-Based In Vivo Imaging and Tumor Targeting (2009)
  • Author(s): K.Minami
  • Organizer: Combinatorial Chemistry and Chemical Biology toward A New Paradigm for Drug Discovery(CCCB)
  • Place of Presentation: Senri Life Science Center(Osaka)
• [Presentation] In Vivo Imaging of N-Glycan Dendrimers by Fluorescence and Positron Emission Tomography(PET) (2009)
  • Author(s): Eric R.O.Siwu
  • Organizer: Combinatorial Chemistry and Chemical Biology toward A New Paradigm for Drug Discovery(CCCB)
  • Place of Presentation: Senri Life Science Center(Osaka)
• [Presentation] In vivo imaging of glycoproteins and glycoclusters (2009)
  • Author(s): Koichi Fukase
  • Organizer: 2nd Switzerland-Japan Biomolecular Chemistry Symposium
  • Place of Presentation: Tokyo Univ.(Tokyo)
• [Presentation] In Vivo Imaging of N-Glycan Dendrimers by Fluorescence and Positron Emission Tomography(PET) (2009)
  • Author(s): Eric R.O.Siwu
  • Organizer: 第29回日本糖質学会年会
  • Place of Presentation: 世界生活文化センター(高山)
• [Presentation] Molecular Probe Method for Studying the Biofunctional Role of Glycoconjugates (2009)
  • Author(s): Koichi Fukase
  • Organizer: The 25th Naito Conference, Chemical Biology II-An Emerging Filed Inspired by Natural Product Chemistry
  • Place of Presentation: Chateraise Gateaux Kingdom(Sapporo)
• [Presentation] Fast Labeling of Lysines Via 6π-Azaelectrocyclization : Application to Pet Imaging of Glycoproteins and Glycociusters (2009)
  • Author(s): Koichi Fukase
  • Organizer: 15th European Carbohydrate Symposium(eurocarb15)
  • Place of Presentation: Vienna, Austria
• [Presentation] In Vivo Imaging of N-Glycan Dendrimers by Fluorescence and Positron Emission Tomography(PET) (2009)
  • Author(s): Eric R.O.Siwu
  • Organizer: 15th European Carbohydrate Symposium(eurocarb15)
  • Place of Presentation: Vienna, Austria
• [Presentation] 糖鎖の合成とin vivo生体分子科学 (2009)
  • Author(s): 第44回天然物化学談話会
  • Organizer: 第44回天然物化学談話会
  • Place of Presentation: つくばグランドホテル(つくば)
• [Presentation] Chemical Glycobiology of Self/Non-self Recognition (2009)
  • Author(s): Koichi Fukase
  • Organizer: Gordon Research Conferences, Carbohydrates
  • Place of Presentation: Tilton, NH
• [Presentation] アスパラギン結合型糖タンパク質糖鎖クラスターの蛍光およびPETイメージング (2009)
  • Author(s): Eric R.O.Siwu1
  • Organizer: 日本ケミカルバイオロジー学会第4回年会
  • Place of Presentation: 神戸市産業振興センター(神戸)
• [Presentation] ポスタータイトル:高速6π-アザ電子環状反応を用いた細胞表層への化学的標識と糖鎖エンジニアリング:細胞のインビボイメージング (2009)
  • Author(s): 南香莉
  • Organizer: 日本ケミカルバイオロジー学会第4回年会
  • Place of Presentation: 神戸市産業振興センター(神戸)
• [Book] Chemical Approach to A Whole Body Imaging of Sialo-N-linked Glycans, In Topics in Current Chemistry volumes, SialoGlyco Chemistry and Biology (2011)
  • Author(s): K. Tanaka, K. Fukase
  • Publisher: Springer
• [Book] 新規標識反応を基盤とする糖鎖プローブの開発とインビボイメージイング,蛍光イメージング/ MRIプローブの開発 (2011)
  • Author(s): 深瀬浩一, 田中克典
  • Publisher: シーエムシー出版
• [Book] ペプチド・タンパク質、細胞の革新的標識法とPETによる動態解析への応用,次世代バイオ医薬品の製剤設計と開発戦略 (2011)
  • Author(s): 田中克典, 深瀬浩一
  • Publisher: シーエムシー出版
• [Book] Chemical Approach to A Whole Body Imaging of Sialo-N-linked Glycans, In Topics in Current Chemistry volumes in SialoGlyco Chemistry and Biology (2011)
  • Author(s): K.Tanaka, K.Fukase
  • Publisher: Springer(in press)
• [Book] 新規標識反応を基盤とする糖鎖プローブの開発とインビボイメージングin蛍光イメーシング・MRIプローブの開発と最新動向 (2011)
  • Author(s): 深瀬浩一, 田中克典
  • Publisher: シーエムシー出版
• [Book] ペプチド・タンパク質、細胞の革新的標識法とPETによる動態解析への応用in次世代バイオ医薬品の製剤設計と開発戦略 (2011)
  • Author(s): 田中克典, 深瀬浩一
  • Publisher: シーエムシー出版
• [Book] Self and non-self recognition with bacterial and animal glycans, surveys by synthetic chemistry, Methods in Enzymology (2010)
  • Author(s): Fujimoto, Y., Tanaka, K., Shimoyama, A., Fukase, K.
  • Total Pages: 478
• [Book] 糖鎖の侵襲的イメージング,「創薬研究への分子イメージング応用」 (2010)
  • Author(s): 田中克典, 深瀬浩一
  • Publisher: メディカルドゥ
• [Book] 糖鎖関連物質の非侵略的インビボイメージングによる診断法開発への展望,『生体の科学』「糖鎖のかかわる病気」 (2010)
  • Author(s): 田中克典, 深瀬浩一
  • Publisher: (財)金原一郎記念医学医療振興財団/医学書院
• [Book] 糖鎖を用いた生体内イメージングとターゲティング, In Vivo Imaging and Targeting by Oligosaccharides and Related Molecules,第三の生命鎖「糖鎖」を活用・制御するケミカルバイオロジー、月刊バイオインダストリー (2010)
  • Author(s): 田中克典, 深瀬浩一
  • Publisher: シーエムシー出版
• [Book] Methods in Enzymology (2010)
  • Author(s): Fujimoto, Y., Fukase, K.
  • Publisher: Glycomics
• [Book] 創薬研究への分子イメージング応用 (2010)
  • Author(s): 田中克典、深瀬浩一
  • Publisher: メディカルドゥ
• [Book] 『生体の科学』「糖鎖のかかわる病気」 (2010)
  • Author(s): 田中克典, 深瀬浩一
  • Publisher: (財)金原一郎記念医学医療振興財団/医学書院
• [Book] 第三の生命鎖「糖鎖」を活用・制御するケミカルバイオロジー、月刊バイオインダストリー (2010)
  • Author(s): 田中克典、深瀬浩一
  • Publisher: シーエムシー出版
• [Book] 『生体の科学』「糖鎖のかかわる病気」 (2010)
  • Author(s): 田中克典, 深瀬浩一
  • Publisher: (財)金原一郎記念医学医療振興財団/医学書院(In press)
• [Book] 糖鎖のインビボバイオイメージング,複合糖質の化学と最新応用技術 (2009)
  • Author(s): 田中克典, 深瀬浩一
  • Publisher: 株式会社シーエムシー出版
• [Remarks]
  • URL: http://www.chem.sci.osaka-u.ac.jp/lab/fukase/
• [Remarks]
  • URL: http://www.chem.sci.osaka-u.ac.jp/lab/fukase/