Establishment of human bone marrow mesenchymal stem cells(hMSCs) culture with serum free medium and evaluation of spinal cord regeneration
| Project/Area Number |
21659356
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Showa University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SHIODA Seiji 昭和大学, 医学部, 教授 (80102375)
OHTAKI Hirokazu 昭和大学, 医学部, 助教 (20349062)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2009: ¥800,000 (Direct Cost: ¥800,000)
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| Keywords | 骨髄幹細胞 / 神経再生 / 脊髄損傷 / 細胞工学 / 骨髄間葉系幹細胞 |
|---|
| Research Abstract |
Although basic research of MSCs has been studied using fetal calf serum-based medium, it is difficult to apply human therapy. Serum-free medium(SFM) might be suitable to use allogenic transplantation of hMSCs in the future. In the present study, we compared characteristics of hMSCs in different media and determined effect and retention of hMSCs after spinal cord injury(SCI) of mice. although SFM decreased adherent, but increased proliferation. Moreover, SFM increased adipocyte differentiation. Transplanted hMSCs improved SCI and migrated toward injury site 6 days after injection.
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Report
(4 results)
Research Products
(120 results)
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[Journal Article] Regulation of Oxidative Stress by Pituitary Adenylate Cyclase-Activating Polypeptide(PACAP) Mediated by PACAP Receptor2010
Author(s)
Ohtaki H, Satoh A, Nakamachi T, Yofu S, Dohi K, Mori H, Ohara K, Miyamoto K, Hashimoto H, Shintani N, Baba A, Matsunaga M, Shioda S.
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Journal Title
J Mol Neurosci
Volume: vol.42
Pages: 397-403
Related Report
Peer Reviewed
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[Journal Article] Regulation of Oxidative Stress by Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) Mediated by PACAP Receptor.2010
Author(s)
Ohtaki H, Satoh A, Nakamachi T, Yofu S, Dohi K, Mori H, Ohara K, Miyamoto K, Hashimoto H, Shintani N, Baba A, Matsunaga M, Shioda S.
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Journal Title
J Mol Neurosci
Volume: 42
Pages: 397-403
Related Report
Peer Reviewed
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[Journal Article] solation of peptide transport system-6 from brain endothelial cells : therapeutic effects with antisense inhibition in Alzheimer and stroke models2009
Author(s)
Dogrukol-Ak D, Kumar VB, Ryerse JS, Farr SA, Verma S, Nonaka N, Nakamachi T, Ohtaki H, Niehoff ML, Edwards JC, Shioda S, Morley JE, Banks WA
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Journal Title
J Cereb Blood Flow Metab
Volume: vol.29
Pages: 411-422
Related Report
Peer Reviewed
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[Journal Article] Isolation of peptide transport system-6 from brain endothelial cells : therapeutic effects with antisense inhibition in Alzheimer and stroke models2009
Author(s)
Dogrukol-Ak D, Kumar VB, Ryerse JS, Farr SA, Verma S, Nonaka N, Nakamachi T, Ohtaki H, Niehoff ML, Edwards JC, Shioda S, Morley JE, Banks WA
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Journal Title
J Cereb Blood Flow Metab 29
Pages: 411-422
Related Report
Peer Reviewed
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[Journal Article] Establishment and characterization of primary adult microglial culture in mice
Author(s)
Ohtaki H, Tsumuraya T, Song D, Sato A, Ohara K, Miyamoto K, Nakano H, Kiriyama K, Dohi K, Hiraizumi Y, Matsunaga M, Shioda S.
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Journal Title
Acta Neurochirurgica Suppl
Volume: (in press)
Related Report
Peer Reviewed
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[Journal Article] Establishment and characterization of primary adult microglial culture in mice
Author(s)
Ohtaki H, Tsumuraya T, Song D, Sato A, Ohara K, Miyamoto K, Nakano H, Kiriyama K, Dohi K, Hiraizumi Y, Matsunaga M, Shioda S
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Journal Title
Acta Neurochirurgica Suppl
Volume: (印刷中)
Related Report
Peer Reviewed
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[Presentation] Suppression of oxidative stress on PACAP might involve in the neuroprotection. Satellite Symposium of the 20th Annual Meeting of the Israel Society for Neuroscience2011
Author(s)
Ohtaki H, Miyamoto K, Tsumuraya T, Kiriyama K, Nakano H, Song D, Nakamachi T, Hiraizumi Y, Matsunaga M, Shioda S
Organizer
Basic and Clinical Research of GPCRs
Place of Presentation
Eilat, Israel
Year and Date
2011-12-13
Related Report
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[Presentation] Suppression of oxidative stress on PACAP might involve in the neuroprotection2011
Author(s)
Ohtaki H, Miyamoto K, Tsumuraya T, Kiriyaia K, Nakano H, Song D, Nakamachi T, Hiraizumi Y, Matsunaga M, Shioda S
Organizer
Satellite Symposium of the 20th Annual Meeting of the Israel Society for Neuroscience Basic and Clinical Research of GPCRs
Place of Presentation
Eilat, Israel
Year and Date
2011-12-13
Related Report
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[Presentation] インターロイキン1の欠損は脊髄損傷のマイクログリア・マクロファージ活性化に影響する2011
Author(s)
大滝博和, 佐藤敦, 小原賢司, 圓谷智海, 加藤慎, 宋丹丹, 小林夏季, 渥美敬, 塩田清二
Organizer
第88回日本生理学会大会,第116回日本解剖学会総会・全国学術集会,合同大会
Place of Presentation
横浜
Related Report
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[Presentation] 職場の腰痛対策2010
Author(s)
平泉裕
Organizer
第12回東京都医師会・昭和大学医師会産業医研修会
Place of Presentation
東京
Year and Date
2010-09-26
Related Report
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[Presentation] The roles of Gp91phox(NOX2) expressed in classical activated microglia after traumatic brain injury2010
Author(s)
Dohi K, Ohtaki H, Kazue S, Nakamachi T, Yofu S, Miyamoto K, Song D, Tsunawaki S, Shioda S, Aruga T
Organizer
10th International Congress of Neuroimmunology(ISNI 2010)
Place of Presentation
Barcelona, Spain
Related Report
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[Presentation] The roles of Gp91phox (NOX2) expressed in classical activated microglia after traumatic brain injury.2010
Author(s)
Dohi K, Ohtaki H, Kazue S, Nakamachi T, Yofu S, Miyamoto K, Song D, Tsunawaki S, Shioda S, Aruga T
Organizer
10th International Congress of Neuroimmunology(ISNI 2010)
Place of Presentation
Barcelona, Spain
Related Report
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