| Project/Area Number |
21659356
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Showa University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SHIODA Seiji 昭和大学, 医学部, 教授 (80102375)
OHTAKI Hirokazu 昭和大学, 医学部, 助教 (20349062)
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| Project Period (FY) |
2009 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2009: ¥800,000 (Direct Cost: ¥800,000)
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| Keywords | 骨髄幹細胞 / 神経再生 / 脊髄損傷 / 細胞工学 / 骨髄間葉系幹細胞 |
|---|
| Research Abstract |
Although basic research of MSCs has been studied using fetal calf serum-based medium, it is difficult to apply human therapy. Serum-free medium(SFM) might be suitable to use allogenic transplantation of hMSCs in the future. In the present study, we compared characteristics of hMSCs in different media and determined effect and retention of hMSCs after spinal cord injury(SCI) of mice. although SFM decreased adherent, but increased proliferation. Moreover, SFM increased adipocyte differentiation. Transplanted hMSCs improved SCI and migrated toward injury site 6 days after injection.
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