Development of ultrasound and bubble-liposome-mediated drug delivery to ocular tissues

• Project/Area Number: 21659400
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Ophthalmology
• Research Institution: Kagoshima University
• Principal Investigator: SAKAMOTO Taiji 鹿児島大学, 医歯学総合研究科, 教授 (10235179)
• Research Collaborator: TACHIBANA Katsuro 福岡大学, 医学部, 教授 ; MARUYAMA Kazuo 帝京大学, 薬学部, 教授
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,140,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥800,000 (Direct Cost: ¥800,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,300,000)
• Keywords: 超音波 / ナノバブル / リポソーム製剤

Research Abstract

We developed a novel method to deliver genes or drugs to ocular tissues using ultrasound and bubble-liposome. GFP cDNA embedded in bubble-liposome was injected in the vitreous followed by irradiation of ultrasound. GFP was strongly expressed by retina where ultrasound was irradiated. Tissue damage was minimal. We also developed a new ultrasound probe for that.

Research Products

• [Journal Article] Selective gene transfer to the retina using intravitreal ultrasound irradiation (2012)
  • Author(s): Sonoda S, Tachibana K, Yamashita T, Shirasawa M, Terasaki H, Uchino E, Suzuki R, Maruyama K, Sakamoto T.
  • Journal Title: J Ophthalmol Volume: Volume2012 Pages: 5-5
  • DOI: 10.1155/2012/412752
  • Peer Reviewed
• [Journal Article] Combination of ultrasound and bubble liposome enhance the effect of doxorubicin and inhibit murine osteosarcoma growth (2011)
  • Author(s): Ueno Y, Sonoda S, Suzuki R, Yokouchi M, Kawasoe Y, Tachibana K, Maruyama K, Sakamoto T, Komiya S.
  • Journal Title: Cancer Biol Ther Volume: 12(4) Pages: 270-277
  • URL: http://dx.doi.org/10.4161/cbt.12.4.16259
  • Peer Reviewed
• [Journal Article] Atypical presentation of acute macular neuroretinopathy with tiny parafoveal reddish-brown lesions (2011)
  • Author(s): Matsuo Y, Uemura A, Nakano T, Inoue M, Sakamoto T.
  • Journal Title: Jpn J Ophthalmol Volume: 55(4) Issue: 4 Pages: 362-364
  • DOI: 10.1007/s10384-011-0028-0
  • NAID: 10029528107
  • Peer Reviewed
• [Journal Article] Ocular surface molecule after transconjunctival vitrectomy (2011)
  • Author(s): Fujita A, Uchino E, Otsuka H, Arimura N, Noda Y, Ishibashi T, Sakamoto T.
  • Journal Title: Br J Ophthalmol Volume: 95(3) Pages: 419-423
  • Peer Reviewed
• [Journal Article] Incidence of iatrogenic peripheral retinal breaks in 23-gauge vitrectomy for macular diseases (2011)
  • Author(s): Nakano T, Uemura A, Sakamoto T
  • Journal Title: Retina Volume: 31(10) Issue: 10 Pages: 1997-2001
  • DOI: 10.1097/iae.0b013e31820f49ea
  • Peer Reviewed
• [Journal Article] Ocular surface molecule after transconjunctival vitrectomy. (2011)
  • Author(s): Fujita A, Uchino E, Otsuka H, Arimura N, Noda Y, Ishibashi T, Sakamoto T.
  • Journal Title: Br J Ophthalmol. Volume: 95(3) Pages: 419-423
  • Peer Reviewed
• [Journal Article] Stromal cell-derived factor-1 is essential for photoreceptor cell protection in retinal detachment (2010)
  • Author(s): Otsuka H, Arimura N, Sonoda S, Nakamura M, Hashiguchi T, Maruyama I, Nakao S, Hafezi-Moghadam A, Sakamoto T.
  • Journal Title: Am J Pathol Volume: 177(5) Pages: 2268-2277
  • URL: http://dx.doi.org/10.2353/ajpath.2010.100134
  • Peer Reviewed
• [Journal Article] Combination therapy for retinal vein occlusion (2010)
  • Author(s): Shimura M, Yasuda K, Nakazawa T, Takeshita T, Shiono T, Sakamoto T.
  • Journal Title: Ophthalmology Volume: 117(9)
  • URL: http://dx.doi.org/10.1016/j.ophtha.2010.03.077
  • Peer Reviewed
• [Journal Article] Aniridia associated with aphakia and secondary glaucoma (2010)
  • Author(s): Terasaki H, Yamashita T, Tanaka M, Takahashi M, Sakamoto T.
  • Journal Title: Jpn J Ophthalmol Volume: 54(5) Issue: 5 Pages: 504-505
  • DOI: 10.1007/s10384-010-0852-7
  • NAID: 10027666075
  • Peer Reviewed
• [Journal Article] Vitrectomy for exudative age-related m acular degeneration with vitreous hemorrh age (2010)
  • Author(s): Sakamoto T, Sheu SJ, Arimura N, Sames hima S, Shimura M, Uemura A, Kawano H, Wu TT, Kubota T, Sohma R, Noda Y.
  • Journal Title: Retina Volume: 30(6) Issue: 6 Pages: 856-864
  • DOI: 10.1097/iae.0b013e3181c969cb
  • Peer Reviewed
• [Journal Article] Predictable signs of benign course of polypoidal choroidal vasculopathy : based upon the long-term observation of non-treated eyes (2010)
  • Author(s): Okubo A, Arimura N, Abematsu N, Sakamoto T.
  • Journal Title: Acta Ophthalmologica Volume: 88(4) Issue: 4
  • DOI: 10.1111/j.1755-3768.2009.01850.x
  • ISSN: 1755-375X, 1755-3768
  • Peer Reviewed
• [Journal Article] B cell-derived vascular endothelial growth factor A promotes lymphangiogenesis and high endothelial venule expansion in lymph nodes (2010)
  • Author(s): Shrestha B, Hashiguchi T, Ito T, Miura N, Takenouchi K, Oyama Y, Kawahara K, Tancharoen S, Ki-I Y, Arimura N, Yoshinaga N, Noma S, Shrestha C, Nitanda T, Kitajima S, Arimura K, Sato M, Sakamoto T, Maruyama I.
  • Journal Title: J Immunol Volume: 184(9) Issue: 9 Pages: 4819-4826
  • DOI: 10.4049/jimmunol.0903063
  • Peer Reviewed
• [Journal Article] Steroid-induced Glaucoma in Children With Acute Lymphoblastic Leukemia : A Possible Complication (2010)
  • Author(s): Yamashita T, Kodama Y, Tanaka M, Yamakiri K, Kawano Y, Sakamoto T.
  • Journal Title: J Glaucoma Volume: 19(3) Issue: 3 Pages: 188-190
  • DOI: 10.1097/ijg.0b013e3181af321d
  • Peer Reviewed
• [Journal Article] Predictable signs of benign course of polypoidal choroidal vasculopathy : based upon the long-term observation of non-treated eyes. (2010)
  • Author(s): Okubo A, Arimura N, Abematsu N, Sakamoto T.
  • Journal Title: Acta Ophthalmol. Volume: 88(4)
  • Peer Reviewed
• [Journal Article] Vitrectomy for exudative age-related macular degeneration with vitreous hemorrhage. (2010)
  • Author(s): Sakamoto T, Sheu SJ, Arimura N, Sameshima S, Shimura M, Uemura A, Kawano H, Wu TT, Kubota T, Sohma R, Noda Y.
  • Journal Title: Retina. Volume: 30(6) Pages: 856-864
  • Peer Reviewed
• [Journal Article] B cell-derived vascular endothelial growth factor A promotes lymphangiogenesis and high endothelial venule expansion in lymph nodes. (2010)
  • Author(s): Shrestha B, Hashiguchi T, Ito T, Miura N, Takenouchi K, Oyama Y, Kawahara K, Tancharoen S, Ki-I Y, Arimura N, Yoshinaga N, Noma S, Shrestha C, Nitanda T, Kitajima S, Arimura K, Sato M, Sakamoto T, Maruyama I.
  • Journal Title: J Immunol. Volume: 184(9) Pages: 4819-4826
  • Peer Reviewed
• [Journal Article] 眼内炎症性疾患の新しい治療 (2010)
  • Author(s): 坂本泰二
  • Journal Title: 学術の動向(日本学術会議) Volume: 7月号 Pages: 14-17
  • NAID: 130000450881
  • Peer Reviewed
• [Journal Article] Aniridia associated with aphakia and secondary glaucoma. (2010)
  • Author(s): Terasaki H, Yamashita T, Tanaka M, Takahashi M, Sakamoto T.
  • Journal Title: Jpn J Ophthalmol. Volume: 54(5) Pages: 504-505
  • NAID: 10027666075
  • Peer Reviewed
• [Journal Article] Steroid-induced glaucoma in children with acute lymphoblastic leukemia : apossible complication. (2010)
  • Author(s): Yamashita T, Kodama Y, Tanaka M, Yamakiri K, Kawano Y, Sakamoto T.
  • Journal Title: J Glaucoma. Volume: 19(3) Pages: 188-190
  • Peer Reviewed
• [Journal Article] 眼内長期滞留ガス(SF6,C3F8)ガイドライン (2010)
  • Author(s): 大路正人, 小椋祐一郎, 坂本泰二, 白神史雄, 平形明人, 寺崎浩子, 東範行, 飯島裕幸, 飯田知弘, 池田恒彦, 石橋達朗, 岸章治, 竹内忍, 中澤満, 根木昭, 堀貞夫, 堀口正之, 松村美代, 山下英俊, 山本修一, 湯澤美都子, 吉田晃敏, 吉村長久
  • Journal Title: 日本眼科学会雑誌 Volume: 114 Pages: 110-115
  • Peer Reviewed
• [Journal Article] Aniridia associated with aphakia and secondary glaucoma (2010)
  • Author(s): Terasaki H, Yamashita T, Tanaka M, Takahashi M, Sakamoto T.
  • Journal Title: Jpn J Ophthalmol. Volume: 54(5) Pages: 504-505
  • NAID: 10027666075
  • Peer Reviewed
• [Journal Article] Stromal cell-derived factor-1 is essential for photoreceptor cell protection in retinal detachment. (2010)
  • Author(s): Otsuka H, Arimura N, Sonoda S, Nakamura M, Hashiguchi T, Maruyama I, Nakao S, Hafezi-Moghadam A, Sakamoto T.
  • Journal Title: Am J Pathol. Volume: 177(5) Pages: 2268-2277
  • Peer Reviewed
• [Journal Article] Combination therapy for retinal vein occlusion. (2010)
  • Author(s): Shimura M, Yasuda K, Nakazawa T, Takeshita T, Shiono T, Sakamoto T.
  • Journal Title: Ophthalmology. Volume: 117(9)
  • Peer Reviewed
• [Journal Article] Steroid-induced Glaucoma in Children With Acute Lymphoblastic Leukemia : A Possible Complication (2010)
  • Author(s): Yamashita T, Kodama Y, Tanaka M, Yamakiri K, Kawano Y, Sakamoto T.
  • Journal Title: J Glaucoma 19(8) Pages: 1617-1624
  • Peer Reviewed
• [Journal Article] Panretinal photocoagulation induces pro-inflammatory cytokines and macular thickening in high-risk proliferative diabetic retinopathy (2009)
  • Author(s): Shimura M, Yasuda K, Nakazawa T, Abe T, Shiono T, Iida T, Sakamoto T, Nishida K.
  • Journal Title: Graefes Arch Clin Exp Ophthalmol Volume: 247(12) Issue: 12 Pages: 1617-1624
  • DOI: 10.1007/s00417-009-1147-x
  • Peer Reviewed
• [Journal Article] Sonothrombolysis for intraocular fibrin formation in an animal model (2009)
  • Author(s): Yamashita T, Ohtsuka H, Arimura N, Sonoda S, Kato C, Ushimaru K, Hara N, Tachibana K, Sakamoto T.
  • Journal Title: Ultrasound Med Biol Volume: 35(11) Pages: 1845-1853
  • URL: http://dx.doi.org/10.1016/j.ultrasmedbio.2009.05.023
  • Peer Reviewed
• [Journal Article] Sonothrombolysis for intraocular fibrin formation in an animal model (2009)
  • Author(s): Yamashita T, Ohtsuka H, Arimura N, Sonoda S, Kato C, Ushimaru K, Hara N, Tachibana K, Sakamoto T.
  • Journal Title: Ultrasound Med Biol 35(11) Pages: 1845-1853
  • Peer Reviewed
• [Journal Article] Panretinal photocoagulation induces pro-inflammatory cytokines and macular thickening in high-risk proliferative diabetic retinopathy (2009)
  • Author(s): Shimura M, Yasuda K, Nakazawa T, Abe T, Shiono T, Iida T, Sakamoto T, Nishida K.
  • Journal Title: Graefes Arch Clin Exp Ophthalmol 247(12) Pages: 1617-1624
  • Peer Reviewed
• [Presentation] 黄斑剥離期間と初回復位の違いによる裂孔原性網膜剥離の視力転帰 (2011)
  • Author(s): 山切啓太, 坂本泰二
  • Organizer: TEAM2011
  • Place of Presentation: 東京国際フォーラム(東京都)
  • Year and Date: 2011-12-03
• [Presentation] トリアムシノロンアセトニド硝子体注射後の非感染性眼内炎 (2011)
  • Author(s): 大塚寛樹, 山下敏史, 園田恭志, 山切啓太, 坂本泰二
  • Organizer: TEAM2011
  • Place of Presentation: 東京国際フォーラム(東京都)
  • Year and Date: 2011-12-02
• [Presentation] 原田病の眼科初診時期と初診時診断 (2011)
  • Author(s): 中尾久美子, 〓松徳子, 水島由佳, 坂本泰二
  • Organizer: 第65回日本臨床眼科学会
  • Place of Presentation: 東京国際フォーラム(東京都)
  • Year and Date: 2011-10-09
• [Presentation] 特発性黄斑円孔手術翌日に詳細な黄斑形態が観察できた症例についての検討 (2011)
  • Author(s): 川野浩輝, 山下敏史, 園田恭志, 山切啓太, 坂本泰二
  • Organizer: 第65回日本臨床眼科学会
  • Place of Presentation: 東京国際フォーラム(東京都)
  • Year and Date: 2011-10-09
• [Presentation] ガス充満眼に対する手術翌日の光干渉断層計検査 (2011)
  • Author(s): 山下敏史, 園田恭志, 山切啓太, 坂本泰二
  • Organizer: 第65回日本臨床眼科学会
  • Place of Presentation: 東京国際フォーラム(東京都)
  • Year and Date: 2011-10-09
• [Presentation] コーヌスを含む視神経乳頭周囲網膜神経線維層厚測定 (2011)
  • Author(s): 喜井裕哉, 山下高明, 田中実, 中尾久美子, 坂本泰二
  • Organizer: 第22回日本緑内障学会
  • Place of Presentation: 秋田ビューホテル・アトリオン(秋田市)
  • Year and Date: 2011-09-24
• [Presentation] インターフェロン治療中に発症した脈絡膜新生血管に対し、ベバシズマブ硝子体内注射が著効した1症例 (2011)
  • Author(s): 寺崎寛人, 園田祥三, 坂本泰二
  • Organizer: 第31回日本眼薬理学会
  • Place of Presentation: ホテル一畑(松江市)
  • Year and Date: 2011-09-17
• [Presentation] ガス下OCTの考察 (2011)
  • Author(s): 坂本泰二
  • Organizer: Japan Macula Club第13回総会
  • Place of Presentation: 蒲郡プリンスホテル(蒲郡市)
  • Year and Date: 2011-08-20
• [Presentation] Early detection of macular hole closure in gas-filled eyes, Special Invited Lecture (2011)
  • Author(s): Sakamoto T.
  • Organizer: 2011 International ongress of the Korean Retina Society
  • Place of Presentation: St Mary's hospital, Seoul, Korea
  • Year and Date: 2011-06-18
• [Presentation] SDF1 is essential for photoreceptor protection in retinal detachment (2011)
  • Author(s): Sakamoto T.
  • Organizer: 2011 International ongress of the Korean Retina Society
  • Place of Presentation: St Mary's hospital, Seoul, Korea
  • Year and Date: 2011-06-18
• [Presentation] Optic disc swelling in Vogt-Ko yanagi-Harada disease (2011)
  • Author(s): Nakao K, Abematsu N, Mizushima Y, Sakamoto T.
  • Organizer: Joint Congress of SOE/AAO
  • Place of Presentation: Geneva, Switzerland
  • Year and Date: 2011-06-04
• [Presentation] Optic disc swelling in Vogt-Koyanagi-Harada disease (2011)
  • Author(s): Nakao K, Abematsu N, Mizushima Y, Sakamoto T
  • Organizer: Joint Congress of SOE/AAO
  • Place of Presentation: Geneva, Switzerland
  • Year and Date: 2011-06-04
• [Presentation] 両眼に発症した頸動脈海綿静脈洞瘻の1例 (2011)
  • Author(s): 喜井裕哉, 田中実, 山下高明, 坂本泰二, 松尾由紀子, 瀬戸弘
  • Organizer: 第81回九州眼科学会
  • Place of Presentation: 沖縄県市町村自治会館(那覇市)
  • Year and Date: 2011-05-28
• [Presentation] NSAIDs点眼による脈絡膜血管新生抑制 (2011)
  • Author(s): 吉永就正, 有村昇, 大塚寛樹, 竹之内和則, 貞村ゆかり, 野間聖, 川原幸一, 園田祥三, 橋口照人, 丸山征郎, 坂本泰二
  • Organizer: 第115回日本眼科学会総会
  • Place of Presentation: 東京国際フォーラム(東京都)
  • Year and Date: 2011-05-13
• [Presentation] 鹿児島県における糖尿病及び糖尿病網膜症に関する疫学調査 (2011)
  • Author(s): 水島崇, 前之原茂穂, 坂本泰二
  • Organizer: 第115回日本眼科学会総会
  • Place of Presentation: 東京国際フォーラム(東京都)
  • Year and Date: 2011-05-13
• [Presentation] マウス脈絡膜新生血管に対する膜蛋白CD9標的薬の血管新生抑制効果 (2011)
  • Author(s): 寺崎寛人, 上笹貫太郎, 小戝健一郎, 坂本泰二
  • Organizer: 第115回日本眼科学会総会
  • Place of Presentation: 東京国際フォーラム(東京都)
  • Year and Date: 2011-05-12
• [Presentation] Assay For Establishment Of Highly Polarized Porcine Retinal Pigment Epithelial Cells (2011)
  • Author(s): Shozo Sonoda, Makoto Shirasawa, Hiroki Otsuka, Toshio Hisatomi, Noboru Arimura, Yasushi Sonoda, Taiji Sakamoto
  • Organizer: ARVO2011
  • Place of Presentation: Fort Lauderdale・U. S. A
  • Year and Date: 2011-05-01
• [Presentation] Assay For Establishment Of Highly Polarized Porcine Retinal Pigment Epithelial Cells (2011)
  • Author(s): Shozo Sonoda, Makoto Shirasawa, Hiroki Otsuka, Toshio Hisatomi, Noboru Arimura, Yasushi Sonoda, Taiji Sakamoto
  • Organizer: ARVO2011
  • Place of Presentation: Fort Lauderdale・U.S.A
  • Year and Date: 2011-05-01
• [Presentation] Chromovitrectomy (2010)
  • Author(s): Taiji Sakamoto
  • Organizer: Yale Eye Center Clinical Conference Series
  • Place of Presentation: U. S. A(New Haven.)
  • Year and Date: 2010-10-22
• [Presentation] Chromovitrectomy. (2010)
  • Author(s): Taiji Sakamoto.
  • Organizer: Yale Eye Center Clinical Conference Series.
  • Place of Presentation: U.S.A(New Haven.)
  • Year and Date: 2010-10-22
• [Presentation] Biology of Vitreous Cavity : Roles of Hyalocytes (2010)
  • Author(s): Taiji Sakamoto
  • Organizer: Yale Eye Center Clinical Conference Series
  • Place of Presentation: U. S. A(New Haven.)
  • Year and Date: 2010-10-21
• [Presentation] Biology of Vitreous Cavity : Roles of Hyalocytes. (2010)
  • Author(s): Taiji Sakamoto.
  • Organizer: Yale Eye Center Clinical Conference Series.
  • Place of Presentation: U.S.A(New Haven.)
  • Year and Date: 2010-10-21
• [Presentation] Non-steroidal anti-inflammatory drug(NSAID) in exudative AMD (2010)
  • Author(s): 坂本泰二
  • Organizer: 第114回日本眼科学会総会
  • Place of Presentation: 名古屋国際会議場(名古屋市)
  • Year and Date: 2010-04-16
• [Presentation] Non-steroidal anti-inflammatory drug(NSAID)in exudative AMD. (2010)
  • Author(s): 坂本泰二
  • Organizer: 第114回日本眼科学会総会
  • Place of Presentation: 名古屋国際会議場(名古屋市)
  • Year and Date: 2010-04-16
• [Presentation] 網膜血管閉塞症について (2009)
  • Author(s): 坂本泰二
  • Organizer: 第11回教育フォーラム
  • Place of Presentation: ホテルオークラ福岡(福岡市)
  • Year and Date: 2009-10-31
• [Presentation] 硝子体生体染色について (2009)
  • Author(s): 坂本泰二
  • Organizer: 第64回日本臨床眼科学会
  • Place of Presentation: 福岡サンパレス(福岡市)
  • Year and Date: 2009-10-12
• [Presentation] Effect of Intravitreal Triamcinolone Acetonide for Polypoidal Choroidal Vasculopathy in Japanese (2009)
  • Author(s): Yoshinaga N, Okubo A, Arimura N, Abematsu N, Sakamoto T.
  • Organizer: Association for Research in Vision and Ophthalmology(ARVO) Annual Meeting
  • Place of Presentation: Fort Lauderdale, U. S. A
  • Year and Date: 2009-05-03
• [Presentation] Vitreous SDF-1 and other cytokines in rhegmatogenous retinal detachment (2009)
  • Author(s): Otsuka H, Arimura N, Yamakiri K, Hashiguchi T, Maruyama I, Sakamoto T.
  • Organizer: Association for Research in Vision and Ophthalmology(ARVO) Annual Meeting
  • Place of Presentation: Fort Lauderdale, U. S. A
  • Year and Date: 2009-05-03
• [Presentation] トリアムシノロン硝子体注入直後の中心窩網膜厚の変化 (2009)
  • Author(s): 園田恭志, 坂本泰二
  • Organizer: 第113回日本眼科学会総会
  • Place of Presentation: 東京国際フォーラム(東京都)
  • Year and Date: 2009-04-17
• [Presentation] Transwell を用いた極性ヒト網膜色素上皮細胞の培養及びその評価 (2009)
  • Author(s): 園田祥三, Parameswaran Sreekumar, 加瀬諭, Christine Spee, Stephan Ryan, Ram Kannan, 坂本泰二, David Hinton
  • Organizer: 第113回日本眼科学会総会
  • Place of Presentation: 東京国際フォーラム(東京都)
  • Year and Date: 2009-04-16
• [Presentation] Effect of Intravitreal Triamcinolone Acetonide for Polypoidal Choroidal Vasculopathy in Japanese (2009)
  • Author(s): Yoshinaga N, Okubo A, Arimura N, Abematsu N, Sakamoto T.
  • Organizer: Association for Research in Vision and Ophthalmology(ARVO)Annual Meeting
  • Place of Presentation: Fort Lauderdale, U.SA
• [Presentation] Vitreous SDF-1 and other cytokines in rhegmatogenous retinal detachment (2009)
  • Author(s): Otsuka H, Arimura N, Yamakiri K, Hashiguchi T, Maruyama I, Sakamoto T.
  • Organizer: Association for Research in Vision and Ophthalmology(ARVO)Annual Meeting
  • Place of Presentation: Fort Lauderdale, U.SA
• [Patent(Industrial Property Rights)] 血管新生抑制剤 (2011)
  • Inventor(s): 小戝健一郎,坂本泰二,上笹貫太郎
  • Industrial Property Rights Holder: 鹿児島大学
  • Industrial Property Number: 2011-510400
  • Filing Date: 2011-10-20
• [Patent(Industrial Property Rights)] 血管新生抑制剤 (2010)
  • Inventor(s): 小戝健一郎,坂本泰二,上笹貫太郎
  • Industrial Property Rights Holder: 鹿児島大学
  • Filing Date: 2010-04-23
  • Overseas