Development of in vivo i ncubator having an ab i I i ty to enhance both the recruitment and the expansion of stem cells as a tool for basic medical researches

• Project/Area Number: 21680042
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Kyoto University
• Principal Investigator: YAMAMOTO Masaya 京都大学, 再生医科学研究所, 准教授 (10332735)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥20,280,000 (Direct Cost: ¥15,600,000、Indirect Cost: ¥4,680,000); Fiscal Year 2011: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2010: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000); Fiscal Year 2009: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
• Keywords: 幹細胞 / 人工ニッチ核酸アプタマー / 核酸アプタマー / 細胞培養基材 / 細胞培養技術 / 人工ニッチ

Research Abstract

Recently many researches have demonstrated the importance of stem cells with a high potential of proliferation and differentiation to realize cell therapy and regenerative medicine. To this end, new technologies and methodologies to recruit and expand stem cells in the body have been developed to accelerate basic researches on cell therapy and regenerative medicine. In particular, both the controlled release of chemoattractant proteins and the immobilization of growth用romoting proteins for stem cells have been investigated.

Research Products

• [Journal Article] Stromal-derived factor-1 delivered via hydrogel drug-delivery vehicle accelerates wound healing in vivo (2011)
  • Author(s): P. W. Henderson, S. P. Singh, D. D. Kri jgh, M. Yamamoto, D. C. Rafii, J. J. Sung, S. Rafii, S. Y. Rabbany, J. A. Spector
  • Journal Title: Wound Repair Regen Volume: 19 Issue: 3 Pages: 420-425
  • DOI: 10.1111/j.1524-475x.2011.00687.x
  • NAID: 10031181758
  • Peer Reviewed
• [Journal Article] Orientation-regulated immobilization of Jaggedl on glass substrates for ex vivo proliferation of a bone marrow cell population containing hematopoietic stem cells (2011)
  • Author(s): H. Toda, M. Yamamoto, H. Kohara, Y. Tabata
  • Journal Title: Biomaterials Volume: 32 Issue: 29 Pages: 6920-6928
  • DOI: 10.1016/j.biomaterials.2011.05.093
  • Peer Reviewed
• [Journal Article] Stromal-derived factor-1 delivered via hydrogel drug-delivery vehicle accelerates wound healing in vivo (2011)
  • Author(s): P.W.Henderson, S.P.Singh, D.D.Krijgh, M.Yamamoto, 他5名
  • Journal Title: Wound Repair and Regeneration Volume: (印刷中)
  • NAID: 10031181758
  • Peer Reviewed
• [Journal Article] Generation of type I collagen gradient in polyacrylamide hydro gels by a simple diffusion-controlled hydrolysis of amide groups (2010)
  • Author(s): M. Yamamoto, K. Yanase, Y. Tabata
  • Journal Title: Materials Volume: 3 Issue: 4 Pages: 2393-2404
  • DOI: 10.3390/ma3042393
  • Peer Reviewed
• [Journal Article] Generation of stable co-cultures of vascular cells in a honeycomb alginate scaffold (2010)
  • Author(s): M. Yamamoto, D. James, H. Li, J. Butler, S. Rafii, S. Rabbany
  • Journal Title: Tissue Eng Part A Volume: 16 Issue: 1 Pages: 299-308
  • DOI: 10.1089/ten.tea.2009.0010
  • Peer Reviewed
• [Journal Article] Continuous delivery of stromal cell-derived factor-1 from alginate scaffolds accelerates wound healing (2010)
  • Author(s): S. Y. Rabbany, J. Pastore, M. Yamamoto, T. Miller, S. Rafii, R. Aras, M. Penn
  • Journal Title: Cell Transplant Volume: 19 Issue: 4 Pages: 399-408
  • DOI: 10.3727/096368909x481782
  • Peer Reviewed
• [Journal Article] Angiogenesis induced by controlled release of neuropeptide substance (2010)
  • Author(s): P. H. Kohara, S. Ta j ima, M. Yamamoto, Y. Tabata
  • Journal Title: Biomaterials Volume: 31 Issue: 33 Pages: 8617-8625
  • DOI: 10.1016/j.biomaterials.2010.07.079
  • Peer Reviewed
• [Journal Article] Mineralization, Biodegradation, and Drug Release Behavior of Gelatin/Apatite Composite Microspheres for Bone Regeneration (2010)
  • Author(s): S.C.Leeuwenburgh, J.Jo, H.Wang, M.Yamamoto, J.A.Jansen, Y.Tabata
  • Journal Title: Biomacromolecules Volume: 11 Pages: 2653-2659
  • Peer Reviewed
• [Journal Article] Continuous delivery of stromal cell-derived factor-1 from alginate scaffolds accelerates wound healing. (2010)
  • Author(s): Y.S.Rabbany, J.Pastore, M.Yamamoto, 他4名
  • Journal Title: Cell Transplantation Volume: 19 Pages: 399-408
  • Peer Reviewed
• [Journal Article] Generation of Stable co-cultures of vascular cells in a honeycomb alginate scaffold. (2010)
  • Author(s): M.Yamamoto, D.James, H.Li, J.Butler, S.Rafii, 他1名
  • Journal Title: Tissue Engineering Part A 16 Pages: 299-308
  • Peer Reviewed
• [Journal Article] Generation of Type I Collagen Gradient in Polyacrylamide Hydrogel by a Simple Diffusion-Controlled Hydrolysis of Amide Groups. (2010)
  • Author(s): M.Yamamoto, K.Yanase, Y.Tabata
  • Journal Title: Materials 3 Pages: 2393-2404
  • Peer Reviewed
• [Journal Article] Continuous delivery of stromal cell-derived factor-1 from alginate scaffolds accelerates wound healing. (2010)
  • Author(s): Y.S.Rabbany, J.Pastore, M.Yamamoto, 他4名
  • Journal Title: Cell Transplantation (印刷中)
  • Peer Reviewed
• [Presentation] Functional Hydrogel Biomaterials for modulation of stem cell behaviors (2012)
  • Author(s): M. Yamamoto
  • Organizer: 2011年度第1回西地区英語セミナー(日本薬剤学会国際委員会主作)
  • Place of Presentation: 京都
  • Year and Date: 2012-04-03
• [Presentation] Sugar-responsive water-solubilization of gelatin hydrogel microspheres in cell aggregate (2011)
  • Author(s): Inoo, K., Yamamoto, M., Tabata,Y.
  • Organizer: The 11th US-Japan Symposium on Drug Delivery System
  • Place of Presentation: Maui, USA
  • Year and Date: 2011-12-16
• [Presentation] Orientation-regulated immobilization of Notch ligand on cell substrates for ex vivo proliferation of a cell population containing hematopoietic stem cell (2011)
  • Author(s): Toda, H., Yamamoto, M., Tabata, Y.
  • Organizer: The 11th US-Japan Symposium on Drug Delivery System
  • Place of Presentation: Maui, USA
  • Year and Date: 2011-12-16
• [Presentation] 再生医療のためのバイオマテリアル.日本材料学会 (2011)
  • Author(s): 山本雅哉
  • Organizer: 第34回材料講習会
  • Place of Presentation: 京都
  • Year and Date: 2011-12-02
• [Presentation] 再生医療のためのバイオマテリアル (2011)
  • Author(s): 山本雅哉
  • Organizer: 日本材料学会第34回材料講習会
  • Place of Presentation: 京都(招待講演)
  • Year and Date: 2011-12-02
• [Presentation] 人工細胞外環境としてのバイオマテリアル.大阪大学蛋白質研究所セミナー (2011)
  • Author(s): 山本雅哉
  • Organizer: 幹細胞を制御する環境因子の分子基盤
  • Place of Presentation: 大阪
  • Year and Date: 2011-11-30
• [Presentation] 人工細胞外環境としてのバイオマテリアル (2011)
  • Author(s): 山本雅哉
  • Organizer: 大阪大学蛋白質研究所セミナー幹細胞を制御する環境因子の分子基盤
  • Place of Presentation: 大阪(招待講演)
  • Year and Date: 2011-11-30
• [Presentation] 硬さの異なるハイドロゲル上へのEphシグナルタンパク質の配向固定化 (2011)
  • Author(s): 戸田裕之、山本雅哉、田畑泰彦
  • Organizer: 第32回日本バイオマテリアル学会大会
  • Place of Presentation: 京都
  • Year and Date: 2011-11-28
• [Presentation] 細胞培養基材としての糖応答性水可溶化ハイドロゲルの作製 (2011)
  • Author(s): 稲生佳菜子、山本雅哉、田畑泰彦
  • Organizer: 第32回日本バイオマテリアル学会大会
  • Place of Presentation: 京都
  • Year and Date: 2011-11-28
• [Presentation] バイオマテリアル分野から見た再生医療の現状 (2011)
  • Author(s): 山本雅哉
  • Organizer: 平成23年度再生医療の全体像を見わたせる分かりやすい解説講座
  • Place of Presentation: 京都
  • Year and Date: 2011-11-08
• [Presentation] Fabrication of cell aggregates with gelatin hydrogel microspheres having the property of sugar-responsive water-solubilization (2011)
  • Author(s): Inoo, K., Yamamoto, M., Tabata, Y.
  • Organizer: Biofabrication 2011
  • Place of Presentation: 富山
  • Year and Date: 2011-10-12
• [Presentation] Fabrication of hydrogels with elasticity changeable by alkaline phosphatase (2011)
  • Author(s): Toda, H., Yamamoto, M., Tabata, Y.
  • Organizer: BMES2011
  • Place of Presentation: Hartford, USA
  • Year and Date: 2011-10-12
• [Presentation] アルカリホスファターゼ刺激により硬さの変化するハイドロゲル培養基材の作製 (2011)
  • Author(s): 戸田裕之、山本雅哉、田畑泰彦
  • Organizer: 第60回高分子討論会
  • Place of Presentation: 岡山
  • Year and Date: 2011-09-28
• [Presentation] 細胞凝集体構築のための糖添加により水可溶化するゼラチンハイドロゲル足場の作製 (2011)
  • Author(s): 稲生佳菜子、山本雅哉、田畑泰彦
  • Organizer: 第60回高分子討論会
  • Place of Presentation: 岡山
  • Year and Date: 2011-09-28
• [Presentation] Morphological change of epithelial cells by stiffness alteration of culture substrates (2011)
  • Author(s): Yamamoto, M., Itooka, T., Tabata, Y.
  • Organizer: The 3rd Asian Biomaterials Congress
  • Place of Presentation: Busan, Korea
  • Year and Date: 2011-09-15
• [Presentation] Design of hydro gels with different stiffness as a culture substrate to control cell behaviors (2011)
  • Author(s): M. Yamamoto, Y. Tabata
  • Organizer: 14^<th> Asian Chemical Congress
  • Place of Presentation: バンコク(タイ)
  • Year and Date: 2011-09-08
• [Presentation] Design of hydrogels with different stiffness as a culture substrate to control cell behaviors (2011)
  • Author(s): Yamamoto, M., Toda, H., Itooka, T., Tabata, Y.
  • Organizer: 14th Asian Chemical Congress
  • Place of Presentation: Bangkok, Thailand(招待講演)
  • Year and Date: 2011-09-08
• [Presentation] ephrinB2-Fcを配向固定化したポリアクリルアミドハイドロゲル培養基材の作製 (2011)
  • Author(s): 戸田裕之、山本雅哉、田畑泰彦
  • Organizer: 日本バイオマテリアル学会第6回関西若手研究発表会
  • Place of Presentation: 大阪
  • Year and Date: 2011-08-12
• [Presentation] 3次元細胞集合体形成のための糖応答性ハイドロゲル足場材料の作製 (2011)
  • Author(s): 稲生佳菜子、山本雅哉、田畑泰彦
  • Organizer: 日本バイオマテリアル学会第6回関西若手研究発表会
  • Place of Presentation: 大阪
  • Year and Date: 2011-08-12
• [Presentation] Preparation of gelatin hydrogel microspheres with the property of sugar-responsive water-solubilization (2011)
  • Author(s): Yamamoto, M., Inoo, K., Tabata, Y.
  • Organizer: Gordon Research Conference on Biomaterials and Tissue Engineering
  • Place of Presentation: Holderness, USA
  • Year and Date: 2011-07-31
• [Presentation] 糖添加により除去可能な細胞足場材料を利用した細胞凝集体の形成 (2011)
  • Author(s): 山本雅哉、稲生佳菜子、田畑泰彦
  • Organizer: 第32回日本炎症・再生医学会
  • Place of Presentation: 京都
  • Year and Date: 2011-06-03
• [Presentation] 細胞シグナルタンパク質を配向固定化した異なる軟らかさをもつハイドロゲルの作製 (2011)
  • Author(s): 戸田裕之、山本雅哉、小原洋志、田畑泰彦
  • Organizer: 第27回日本炎症・再生医学会
  • Place of Presentation: 京都
  • Year and Date: 2011-06-02
• [Presentation] アルカリホスファターゼに応答し硬さの変化するハイドロゲルの作製 (2011)
  • Author(s): 戸田裕之、山本雅哉、田畑泰彦
  • Organizer: 第60回高分子年次大会
  • Place of Presentation: 大阪
  • Year and Date: 2011-05-27
• [Presentation] 糖に応答して柔らかさの変化するハイドロゲル足場の作製 (2011)
  • Author(s): 糸岡朝樹、山本雅哉、田畑泰彦
  • Organizer: 第60回高分子年次大会
  • Place of Presentation: 大阪
  • Year and Date: 2011-05-27
• [Presentation] 糖に応答して水可溶化するゼラチンハイドロゲル粒子の作製 (2011)
  • Author(s): 稲生佳菜子、山本雅哉、田畑泰彦
  • Organizer: 第60回高分子年次大会
  • Place of Presentation: 大阪
  • Year and Date: 2011-05-27
• [Presentation] バイマテリアル・DDSを中心とした再生医療に必要なモノづくり (2011)
  • Author(s): 山本推哉
  • Organizer: 再生医療分野モノづくりシンポジウム
  • Place of Presentation: 京都
  • Year and Date: 2011-02-10
• [Presentation] 細胞周辺環境としての機能性バイオマテリアル足場の開発 (2010)
  • Author(s): 山本雅哉
  • Organizer: 第32回日本バイオマテリアル学会大会平成22年度日本バイオマテリアル学会
  • Place of Presentation: 広島
  • Year and Date: 2010-11-29
• [Presentation] 糖添加により水可溶化するゼラチンハイドロゲルの作製 (2010)
  • Author(s): 稲生佳菜子, 山本雅哉, 田畑泰彦
  • Organizer: 第32回日本バイオマテリアル学会大会
  • Place of Presentation: 広島
  • Year and Date: 2010-11-29
• [Presentation] アルカリホスファターゼにより硬さの変化するハイドロゲルの作製 (2010)
  • Author(s): 戸田裕之, 山本雅哉, 田畑泰彦
  • Organizer: 第32回日本バイオマテリアル学会大会
  • Place of Presentation: 広島
  • Year and Date: 2010-11-29
• [Presentation] 再生医療におけるバイオマテリアルの重要性 (2010)
  • Author(s): 山杢錐哉
  • Organizer: 福井大学ものづくり講演会
  • Place of Presentation: 福井
  • Year and Date: 2010-11-26
• [Presentation] Biomolecules-functionalized scaffolds fo rstem celgrowth and differentiation (2010)
  • Author(s): M. Yamamoto, Y. Tabata
  • Organizer: ISOMRM2010
  • Place of Presentation: 台北(台湾)
  • Year and Date: 2010-11-05
• [Presentation] Morphological change of epithelial cells cultured on substrates with different stiffness (2010)
  • Author(s): M.Yamamoto, Y.Murakami, Y.Tabata
  • Organizer: Annual Meeting of Biomedical Engineering Society
  • Place of Presentation: オースチン
  • Year and Date: 2010-10-06
• [Presentation] 糖応答性をもつ生体吸収性ハイドロゲルの作製 (2010)
  • Author(s): 稲生佳菜子, 山本雅哉, 田畑泰彦
  • Organizer: 日本バイオマテリアル学会第5回関西若手研究発表会
  • Place of Presentation: 京都
  • Year and Date: 2010-08-06
• [Presentation] 軟らかさの異なる糖応答性ハイドロゲル上での上皮細胞の上皮間葉転換 (2010)
  • Author(s): 山本雅哉, 村上祐子, 田畑泰彦
  • Organizer: 第31回日本炎症・再生医学会
  • Place of Presentation: 東京
  • Year and Date: 2010-08-05
• [Presentation] 糖応答性をもつフェニルボロン酸導入ハイドロゲルの調製 (2010)
  • Author(s): 稲生佳菜子, 山本雅哉, 田畑泰彦
  • Organizer: 第56回高分子研究発表会
  • Place of Presentation: 神戸
  • Year and Date: 2010-07-16
• [Presentation] 細胞培養基材としての高分子ハイドロゲルの設計 (2010)
  • Author(s): 山本雅哉, 林健太朗, 村上裕子, 稲生佳菜子, 田畑泰彦
  • Organizer: 第136回ポバール会
  • Place of Presentation: 京都
  • Year and Date: 2010-07-03
• [Presentation] 徐放化骨形成因子を組み込んだ機能性バイオマテリアルを用いた放射線照射骨欠損の再生誘導 (2010)
  • Author(s): 山本雅哉
  • Organizer: 第2回日本再生医療学会YIA受賞者講演
  • Place of Presentation: 広島
  • Year and Date: 2010-03-18
• [Presentation] 機能性足場材料の作製に利用可能な表面ならびに三次元加工技術 (2010)
  • Author(s): 山本雅哉
  • Organizer: 京都リサーチパーク第6回再生医療サポートビジネス懇話会
  • Place of Presentation: 京都
  • Year and Date: 2010-02-19
• [Presentation] 多孔質バイオマテリアルを利用した3次元脈管組織モデルの構築 (2010)
  • Author(s): 山本雅哉
  • Organizer: 第1回Bio-creation研究会
  • Place of Presentation: 松山
  • Year and Date: 2010-01-25
• [Presentation] Osteoblastic differentiation of mesenchymal stem cells in alginate scaffolds with a three-dimensional spatial gradient of biomolecules. (2009)
  • Author(s): Yamamoto, M., Tabata, Y.
  • Organizer: Kyoto University Global COE program Center for Frontier Medicine Retreat 2009
  • Place of Presentation: 淡路島
  • Year and Date: 2009-11-07