Functional analysis of mechanical stress-induced microRNAs
| Project/Area Number |
21680049
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Sports science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
AKIMOTO Takayuki The University of Tokyo, 大学院・医学系研究科, 講師 (00323460)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥27,950,000 (Direct Cost: ¥21,500,000、Indirect Cost: ¥6,450,000)
Fiscal Year 2010: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
Fiscal Year 2009: ¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
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| Keywords | 筋肉 / 運動 / 細胞内シクナル伝達 / 物理的ストレス / 転写調節 / スモールRNA / 骨格筋 / 関節軟骨 / メカニカルストレス / 物理刺激 / 細胞分化 / 組織成熟 / ノンコーディングRNA / マイクロRNA / 転写因子 |
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| Research Abstract |
The aim of this study is to determine gene regulation related to physical exercise, especially focusing on post-transcriptional control due to mechanical stress-induced microRNA (miRNA)s. We first identified several mechanical stress-induced miRNAs by a comprehensive microarray analysis. Among them, we chose miR-23a to analyze with respect to its function in skeletal muscle by using cultured cells and experimental animals. We found that miR-23a inhibits skeletal muscle atrophy by suppressing the translation of two ubiquitin ligases, atrogin-1 and MuRF1 in their 3'UTR dependent manner.
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Report
(3 results)
Research Products
(14 results)
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[Journal Article] The Mohawk homeobox gene plays a critical role in tendon differentiation by regulating type I collagen production.2010
Author(s)
Y.Ito, N.Toriuchi, T.Yoshitaka, H.Ueno-Kudoh, T.Sato, S.Yokoyama, K.Nishida, T.Akimoto, M.Takahashi, S.Miyaki, H.Asahara.
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Journal Title
Proc Natl Acad Sci USA. 107(23)
Pages: 10538-10542
Related Report
Peer Reviewed
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[Journal Article] The Mohawk homeobox gene plays a critical role in tendon differentiation by regulating type I collagen production.2010
Author(s)
Y.Ito, N.Toriuchi, T.Yoshitaka, H.Ueno-Kudoh, T.Sato, S.Yokoyama, K.Nishida, T.Akimoto, M.Takahashi, S.Miyaki, H.Asahara.
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Journal Title
Proc Natl Acad Sci U S A.
Volume: 107
Pages: 10538-10542
Related Report
Peer Reviewed
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[Presentation] Translational suppression of atrophic regulators by miR-23a integrates resistance to skeletal muscle atrophy.2010
Author(s)
S.Wada, Y.Kato, M.Okutsu, S.Miyaki, K.Suzuki, Z.Yan, S.Schiaffino, H.Asahara, T.Ushida, T.Akimoto.
Organizer
European Muscle Conference
Place of Presentation
Abano-Termae(Padova), Italy
Year and Date
2010-09-14
Related Report
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[Presentation] Translational suppressions of atrophic regulators by a microRNA integrate resistance against skeletal muscle atrophy2010
Author(s)
S.Wada, Y.Kato, M.Okutsu, S.Miyaki, K.Suzuki, Z.Yan, S.Schiaffino, H.Asahara, T.Ushida, T.Akimoto
Organizer
EMC 2010
Place of Presentation
Padova, Italy
Year and Date
2010-09-14
Related Report
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