Functional analysis of mechanical stress-induced microRNAs

• Project/Area Number: 21680049
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Sports science
• Research Institution: The University of Tokyo
• Principal Investigator: AKIMOTO Takayuki The University of Tokyo, 大学院・医学系研究科, 講師 (00323460)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥27,950,000 (Direct Cost: ¥21,500,000、Indirect Cost: ¥6,450,000); Fiscal Year 2010: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000); Fiscal Year 2009: ¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
• Keywords: 筋肉 / 運動 / 細胞内シクナル伝達 / 物理的ストレス / 転写調節 / スモールRNA / 骨格筋 / 関節軟骨 / メカニカルストレス / 物理刺激 / 細胞分化 / 組織成熟 / ノンコーディングRNA / マイクロRNA / 転写因子

Research Abstract

The aim of this study is to determine gene regulation related to physical exercise, especially focusing on post-transcriptional control due to mechanical stress-induced microRNA (miRNA)s. We first identified several mechanical stress-induced miRNAs by a comprehensive microarray analysis. Among them, we chose miR-23a to analyze with respect to its function in skeletal muscle by using cultured cells and experimental animals. We found that miR-23a inhibits skeletal muscle atrophy by suppressing the translation of two ubiquitin ligases, atrogin-1 and MuRF1 in their 3'UTR dependent manner.

Research Products

• [Journal Article] Acute exercise activates local bioactive androgen metabolism in skeletal muscle. (2010)
  • Author(s): K.Aizawa, M.Iemitsu, S.Maeda, T.Otsuki, K.Sato, T.Ushida, N.Mesaki, T.Akimoto.
  • Journal Title: Steroid 75(3) Pages: 219-223
  • Peer Reviewed
• [Journal Article] The Mohawk homeobox gene plays a critical role in tendon differentiation by regulating type I collagen production. (2010)
  • Author(s): Y.Ito, N.Toriuchi, T.Yoshitaka, H.Ueno-Kudoh, T.Sato, S.Yokoyama, K.Nishida, T.Akimoto, M.Takahashi, S.Miyaki, H.Asahara.
  • Journal Title: Proc Natl Acad Sci USA. 107(23) Pages: 10538-10542
  • Peer Reviewed
• [Journal Article] The Mohawk homeobox gene plays a critical role in tendon differentiation by regulating type I collagen production. (2010)
  • Author(s): Y.Ito, N.Toriuchi, T.Yoshitaka, H.Ueno-Kudoh, T.Sato, S.Yokoyama, K.Nishida, T.Akimoto, M.Takahashi, S.Miyaki, H.Asahara.
  • Journal Title: Proc Natl Acad Sci U S A. Volume: 107 Pages: 10538-10542
  • Peer Reviewed
• [Journal Article] Acute exercise activates local bioactive androgen metabolism in skeletal muscle. (2010)
  • Author(s): K.Aizawa, M.Iemitsu, S.Maeda, T.Otsuki, K.Sato, T.Ushida, N.Mesaki, T.Akimoto
  • Journal Title: Steroids Volume: 75 Pages: 219-223
  • Peer Reviewed
• [Journal Article] Exercise training activates local bioactive androgen metabolism in skeletal muscle (2010)
  • Author(s): K.Aizawa, M.Iemitsu, S.Maeda, T.Otsuki, K.Sato, T.Ushida, N.Mesaki, T.Akimoto
  • Journal Title: Steroids 75 Pages: 219-223
  • Peer Reviewed
• [Journal Article] Effects of acute hypoxia on metabolic and hormonal responses to resistance exercise (2010)
  • Author(s): M.Kon, T.Ikeda, T.Homma, T.Akimoto, Y.Suzuki, T.Kawahara
  • Journal Title: Med Sci Sports Exerc (掲載確定(印刷中))
  • Peer Reviewed
• [Journal Article] Endurance exercise training enhances local sex steroidogenesis in skeletal muscle.
  • Author(s): K.Aizawa, M.Iemitsu, S.Maeda, N.Mesaki, T.Ushida, T.Akimoto.
  • Journal Title: Med Sci Sports Exerc,掲載確定
  • Peer Reviewed
• [Presentation] 骨格筋における活性型アンドロゲン産生の調節機序 (2010)
  • Author(s): 相澤勝治, 和田正吾, 目崎登, 牛田多加志, 秋本崇之
  • Organizer: 第65回日本体力医学会学術総会
  • Place of Presentation: 千葉商科大学(千葉)
  • Year and Date: 2010-09-17
• [Presentation] Translational suppression of atrophic regulators by miR-23a integrates resistance to skeletal muscle atrophy. (2010)
  • Author(s): S.Wada, Y.Kato, M.Okutsu, S.Miyaki, K.Suzuki, Z.Yan, S.Schiaffino, H.Asahara, T.Ushida, T.Akimoto.
  • Organizer: European Muscle Conference
  • Place of Presentation: Abano-Termae(Padova), Italy
  • Year and Date: 2010-09-14
• [Presentation] Translational suppressions of atrophic regulators by a microRNA integrate resistance against skeletal muscle atrophy (2010)
  • Author(s): S.Wada, Y.Kato, M.Okutsu, S.Miyaki, K.Suzuki, Z.Yan, S.Schiaffino, H.Asahara, T.Ushida, T.Akimoto
  • Organizer: EMC 2010
  • Place of Presentation: Padova, Italy
  • Year and Date: 2010-09-14
• [Presentation] メカニカルストレスによるPGC-1αアイソフォームの発現 (2009)
  • Author(s): 秋本崇之, 和田正吾, 相澤勝治, 牛田多加志
  • Organizer: 第64回日本体力医学会学術総会
  • Place of Presentation: 朱鷺メッセ(新潟)
  • Year and Date: 2009-09-18
• [Presentation] マイクロRNAによる骨格筋の制御.ンポジウム10「The Myogenesis-遺伝子・細胞・組織から検討した骨格筋細胞の発生・分化・再生-」 (2009)
  • Author(s): 秋本崇之, 和田正吾, 加藤義雄, 浅原弘嗣, 牛田多加志
  • Organizer: 第64回日本体力医学会学術総会
  • Place of Presentation: 朱鷺メッセ(新潟)
  • Year and Date: 2009-09-18
• [Presentation] メカニカルストレスによるPGC-1αアイソフォームの発現 (2009)
  • Author(s): 秋本崇之, 和田正吾, 相澤勝治, 牛田多加志
  • Organizer: 日本体力医学会
  • Place of Presentation: 新潟, 朱鷺メッセ
  • Year and Date: 2009-09-18
• [Book] 物理的刺激と軟骨細胞(運動器慢性疾患に対する運動療法)(分担執筆:黒澤尚編) (2009)
  • Author(s): 秋本崇之, 牛田多加志
  • Publisher: 金原出版,東京