Comprehensive functional screening and application of cytochrome P450 from basidiomycetes

• Project/Area Number: 21688013
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Wood science
• Research Institution: Kyushu University
• Principal Investigator: ICHINOSE Hirofumi 九州大学, 大学院・農学研究院, 助教 (00432948)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥28,340,000 (Direct Cost: ¥21,800,000、Indirect Cost: ¥6,540,000); Fiscal Year 2011: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000); Fiscal Year 2010: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000); Fiscal Year 2009: ¥13,000,000 (Direct Cost: ¥10,000,000、Indirect Cost: ¥3,000,000)
• Keywords: 木材腐朽担子菌・シトクロムP450・リグニン / シトクロムP450 / 微生物 / バイオテクノロジー / バオテクノロジー

Research Abstract

We explored the molecular diversity of basidiomycetous cytochrome P450 and isolated 304 genes encoding full-length cDNA. The isolated P450 genes were transformed into Saccharomyces cerevisiae to construct a functional library. Using the functional library, the catalytic potentials of P450s against a wide variety of compounds were elucidated. A functionomic survey allowed the discovery of novel catalytic potentials. An increased compilation of P450 functions will facilitate a thorough understanding of metabolic diversity in basidiomycetes and provide new insights that could also expedite practical applications in the biotechnology sector.

Research Products

• [Journal Article] 2. Molecular identification and functional characterization of cytochrome P450 monooxygenases from the brown-rot basidiomycete Postia placenta (2012)
  • Author(s): Ide M, Ichinose H, Wariishi H.
  • Journal Title: Wariishi H. Arch Microbiol Volume: 194(4) Pages: 243-53
  • Peer Reviewed
• [Journal Article] Heterologous expression and mechanistic investigation of a fungal cytochrome P450(CYP5150A2): Involvement of alternative redox partners (2012)
  • Author(s): Ichinose H., Wariishi H.
  • Journal Title: Arch Biochem Biophys Volume: 518(1) Pages: 8-15
  • Peer Reviewed
• [Journal Article] Molecular identification and functional characterization of cytochrome P450 monooxygenases from the brown-rot basidiomycete Postia placenta (2012)
  • Author(s): Ide M., et al
  • Journal Title: Archives of Microbiology Volume: 194 Issue: 4 Pages: 243-253
  • DOI: 10.1007/s00203-011-0753-2
  • Peer Reviewed
• [Journal Article] Heterologous expression and mechanistic investigation of a fungal cytochrome P450 (CYP5150A2) : Involvement of alternative redox partners (2012)
  • Author(s): Ichinose H., et al
  • Journal Title: Archives of Biochemistry and Biophisics Volume: 518 Issue: 1 Pages: 8-15
  • DOI: 10.1016/j.abb.2011.12.010
  • Peer Reviewed
• [Journal Article] Molecular and functional diversity of fungal cytochrome P450S (2012)
  • Author(s): Ichinose H
  • Journal Title: Biological and Pharmaceutical Bulletin Volume: 35(in press)
  • NAID: 130001872161
  • Peer Reviewed
• [Journal Article] Construction and application of a functional library of cytochrome P450 monooxygenases from the filamentous fungus Aspergillus oryzae. Nazir KHMNH (2011)
  • Author(s): Nazir KHMNH, Ichinose H, Wariishi H.
  • Journal Title: Appl Environ Microbiol Volume: 77(9) Pages: 3147-50
  • Peer Reviewed
• [Journal Article] Insight into functional diversity of cytochrome P450 in the white-rot basidiomycete Phanerochaete chrysosporium : Involvement of versatile monooxygenase (2011)
  • Author(s): Hirosue S, Tazaki M, Hiratsuka N, Yanai S, Kabumoto H, Shinkyo R, Arisawa A, Sakaki T, Tsunekawa H, Johdo O, Ichinose H, Wariishi H
  • Journal Title: Biochem Biophys Res Commun Volume: 407(1) Pages: 118-23
  • Peer Reviewed
• [Journal Article] Construction and application of a functional library of cytochrome P450 monooxygenases from the filamentous fungus Aspergillus oryzae. (2011)
  • Author(s): Nazir KHMNH, et al.
  • Journal Title: Applied and Environmental Microbiology Volume: (in press)
  • Peer Reviewed
• [Journal Article] Insight into functional diversity of cytochrome P450 in the white-rot basidiomycete Phanerochaete chrysosporium : Involvement of versatile monooxygenase. (2011)
  • Author(s): Hirosue S, et al.
  • Journal Title: Biochemistry Biophysics Research Communications Volume: 407 Pages: 118-23
  • Peer Reviewed
• [Journal Article] Cytochrome P450 monooxygenases involved in anthracene metabolism by the white-rot basidiomycete Phanerochaete chrysosporium (2010)
  • Author(s): Chigu NL, Hirosue S, Nakamura C, Teramoto H, Ichinose H, Wariishi H
  • Journal Title: Appl Microbiol Biotechnol Volume: 87(5) Pages: 1907-16
  • Peer Reviewed
• [Journal Article] Molecular characterization and isolation of cytochrome P450 genes from the filamentous fungus Aspergillus oryzae (2010)
  • Author(s): Nazir KHMNH, Ichinose H, Wariishi H.
  • Journal Title: Arch Microbiol Volume: 192(5) Pages: 395-408
  • Peer Reviewed
• [Journal Article] Metabolism of mono-and dichloro-dibenzop-dioxins by Phanerochaete chrysosporium cytochromes P450 (2010)
  • Author(s): Kasai N, Ikushiro S, Shinkyo R, Yasuda K, Hirosue S, Arisawa A, Ichinose H, Wariishi H, Sakaki T
  • Journal Title: Appl Microbiol Biotechnol Volume: 86(2) Pages: 773-80
  • Peer Reviewed
• [Journal Article] Atypical kinetics of cytochromes P450 catalysing 3'-hydroxylation of flavone from the white-rot fungus Phanerochaete 0 5 10 15 20 0. 00 0. 02 0. 04 0. 06 0. 08 0. 10[ PBA](μM)! Absorbance! Absorbance Wavelength(nm) 400 550 0. 04 0. 02-0. 02 350 450 500-0. (2010)
  • Author(s): Kasai N, Ikushiro S, Hirosue S, Arisawa A, Ichinose H, Uchida Y, Wariishi H, Ohta M, Sakaki T
  • Journal Title: J Biochem. Volume: 147(1) Pages: 117-25
  • Peer Reviewed
• [Journal Article] Cytochrome P450 monooxygenases involved in anthracene metabolism by the white-rot basidiomycete Phanerochaete chrysosporium. (2010)
  • Author(s): Chigu NL, et al.
  • Journal Title: Applied Microbiology and Biotechnology Volume: 87 Pages: 1907-1916
  • Peer Reviewed
• [Journal Article] Metabolism of mono- and dichloro-dibenzo-p-dioxins by Phanerochaete chrysosporium cytochromes P450 (2010)
  • Author(s): Kasai, et al.
  • Journal Title: Applied Microbiology and Biotechnology 86 Pages: 773-780
  • Peer Reviewed
• [Journal Article] Atypical kinetics of cytochromes P450 catalysing 3'-hydroxylation of flavone from the white-rot funeus Phanerochaete chrysosporium (2010)
  • Author(s): Kasai, et al.
  • Journal Title: Journal of Biochemistry 147 Pages: 117-125
  • Peer Reviewed
• [Journal Article] 10. Enzymatic properties of cytochrome P450 catalyzing 3'-hydroxylation of naringenin from the white-rot fungus Phanerochaete chrysosporium (2009)
  • Author(s): Kasai N, Ikushiro S, Hirosue S, Arisawa A, Ichinose H, Wariishi H, Ohta M, Sakaki T
  • Journal Title: Biochem Biophys Res Commun Volume: 387(1)] Pages: 103-8
  • Peer Reviewed
• [Journal Article] Enzymatic properties of cytochrome P450 catalyzing 3'-hydroxylation of naringenin from the white-rot fungus Phanerochaete chrysosporium (2009)
  • Author(s): Kasai, et al.
  • Journal Title: Biochemical and Biophysical Research Communications 387 Pages: 103-108
  • Peer Reviewed
• [Journal Article] Molecular and functional diversity of fungal cytochrome P450s.
  • Author(s): Ichinose H
  • Journal Title: Biol. Pharm. Bull Volume: (in press)
  • NAID: 130001872161
  • Peer Reviewed
• [Journal Article] Molecular characterization and isolation of cytochrome P450 genes from the filamentous fungus Aspergillus oryzae
  • Author(s): Nazir, et al.
  • Journal Title: Archives of Microbiology (in press)
  • Peer Reviewed
• [Presentation] 真核微生物が有するシトクロムP450の機能スクリーニング (2012)
  • Author(s): 眞田有規, ら
  • Organizer: 第62回日本木材学会大会
  • Place of Presentation: 北海道大学(札幌)
  • Year and Date: 2012-03-15
• [Presentation] Molecular diversity and functional application of cytochrome P450 from basidiomycetes (2011)
  • Author(s): Ichinose H.
  • Organizer: The 6th International Medicinal Mushrooms Conference
  • Place of Presentation: Zagreb, Croatia
  • Year and Date: 2011-09-27
• [Presentation] Molecular diversity and functional application of cytochrome P450 from basidiomycetes (2011)
  • Author(s): Ichinose H
  • Organizer: The 6th International Medicinal Mushrooms Conference
  • Place of Presentation: ザグレブ(クロアチア)
  • Year and Date: 2011-09-27
• [Presentation] 真核微生物が有するシトクロムP450 :機能解明と高度利用 (2011)
  • Author(s): 一瀬博文
  • Organizer: 第84回日本生化学会大会(シンポジウム)
  • Place of Presentation: 京都
  • Year and Date: 2011-09-24
• [Presentation] 真核微生物が有するシトクロムP450:機能解明と高度利用 (2011)
  • Author(s): 一瀬博文
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都国際会館(京都)(招待講演)
  • Year and Date: 2011-09-24
• [Presentation] Functionomic survey and catalytic application of cytochrome P450 monooxygenases from fungi (2011)
  • Author(s): Ichinose H., Wariishi H.
  • Organizer: 17th International Conference on Cytochrome P450 Biochemistry, Biophysics and Structure
  • Place of Presentation: Manchester, UK
  • Year and Date: 2011-06-26
• [Presentation] Functionomic survey and catalytic application of cyto chrome P450 monooxygenases from fungi (2011)
  • Author(s): Ichinose H., Warlishi H.
  • Organizer: 17th International Conference on Cytochrome P450 Biochemistry, Biophysics and Structure
  • Place of Presentation: マンチェスター(英国)
  • Year and Date: 2011-06-26
• [Presentation] Functional application of fungal cytochrome P450 for bioindustry (2011)
  • Author(s): Ichinose H
  • Organizer: 33rd Symposium on Biotechnology for Fuels and Chemicals
  • Place of Presentation: シアトル(英国)
  • Year and Date: 2011-05-22
• [Presentation] Functional application of fungal cytochrome P450 for bioindustry (2011)
  • Author(s): Ichinose H.
  • Organizer: 33rd Symposium on Biotechnology for Fuels and Chemicals
  • Place of Presentation: Seattle USA
  • Year and Date: 2011-05-02
• [Presentation] 担子菌が有するシトクロムP450機能の解明と利用 (2011)
  • Author(s): 井手正迪,一瀬博文,割石博之
  • Organizer: 第61回日本木材学会大会
  • Place of Presentation: 京都
  • Year and Date: 2011-03-19
• [Presentation] 真核微生物が有するシトクロムP450の機能スクリーニング (2011)
  • Author(s): 眞田有規,一瀬博文,割石博之
  • Organizer: 第62回日本木材学会大会
  • Place of Presentation: 札幌
  • Year and Date: 2011-03-15
• [Presentation] Functional characterization of Cytochromes P450 from the Filamentous Fungus Aspergillus oryzae (2010)
  • Author(s): Nazir KHMNH, et al.
  • Organizer: 10th International Symposium on Cytochrome P450 Biodiversity and Biotechnology
  • Place of Presentation: 米国・マサチューセッツ
  • Year and Date: 2010-10-03
• [Presentation] Molecular identification and functional characterization of cytochromes P450 from wood-rotting fungi (2010)
  • Author(s): Ide Masamichi, et al
  • Organizer: 10th International Symposium on Cytochrome P450 Biodiversity and Biotechnology
  • Place of Presentation: 米国・マサチューセッツ
  • Year and Date: 2010-10-03
• [Presentation] Molecular and functional characterization of cytochromes P450 from the filamentous fungus Aspergillus oryzae (2010)
  • Author(s): Nazir KHMNH., Ichinose H., Wariishi H.
  • Organizer: IMC9 The Biology of Fungi
  • Place of Presentation: Edinburgh UK
  • Year and Date: 2010-08-01
• [Presentation] Molecular diversity of cytochromes P450 from wood-rotting basidiomycetes (2010)
  • Author(s): Ide M., Ichinose H., Wariishi H.
  • Organizer: IMC9 The Biology of Fungi
  • Place of Presentation: Edinburgh UK
  • Year and Date: 2010-08-01
• [Presentation] Molecular and functional characterization of cytochromes P450 from the filamentous fungus Aspergillus oryzae (2010)
  • Author(s): Nazir KHMNH, et al.
  • Organizer: IMC9 The Biology of Fungi
  • Place of Presentation: 英国・エジンバラ
  • Year and Date: 2010-08-01
• [Presentation] Molecular diversity of cytochromes P450 from wood-rotting basidiomycetes (2010)
  • Author(s): Ide Masamichi, et al.
  • Organizer: IMC9 The Biology of Fungi
  • Place of Presentation: 英国・エジンバラ
  • Year and Date: 2010-08-01
• [Presentation] Functional characterization of cytochromes P450 from the filamentous fungus Aspergillus oryzae (2010)
  • Author(s): Nazir KHMN., Ichinose H., Wariishi H.
  • Organizer: The 10th International Symposium on Cytochrome P450 Biodiversity and Biotechnology
  • Place of Presentation: Boston USA
  • Year and Date: 2010-03-20
• [Presentation] Molecular identification and functional characterization of cytochromes P450 from wood-rotting fungi (2010)
  • Author(s): Ide M., Ichinose H., Wariishi H.
  • Organizer: The 10th International Symposium on Cytochrome P450 Biodiversity and Biotechnolog
  • Place of Presentation: Boston USA
  • Year and Date: 2010-03-20
• [Presentation] 褐色腐朽担子菌シトクロムP450のクローニングおよび異種発現 (2010)
  • Author(s): 井手正迪,一瀬博文,割石博之
  • Organizer: 第60回日本木材学会大会
  • Place of Presentation: 宮崎
  • Year and Date: 2010-03-17
• [Presentation] Phanerochaete chrysosporiumが有するクロロペルオキシダーゼ遺伝子の単離および同定 (2010)
  • Author(s): 嶋岡隆行,一瀬博文,割石博之
  • Organizer: 第60回日本木材学会大会
  • Place of Presentation: 宮崎
  • Year and Date: 2010-03-17
• [Presentation] Functional diversity and enzymatic application of cytochrome P450 from wood-rotting basidiomycetes (2009)
  • Author(s): Ichinose H., Hirosue H., Tazaki M., Arisawa A., Jodo O., Wariishi H.
  • Organizer: Asia pacific biochemical engineering conference
  • Place of Presentation: Kobe Japan
  • Year and Date: 2009-11-25
• [Presentation] Functional diversity and enzymatic application of cytochrome P450 from wood-rotting basidiomycetes (2009)
  • Author(s): Ichinose, et al.
  • Organizer: Asia pacific biochemical engineering conference 2009
  • Place of Presentation: Kobe
  • Year and Date: 2009-11-25
• [Presentation] 木材腐朽担子菌におけるシトクロムP450分子種の多様性 (2009)
  • Author(s): 井手正迪,一瀬博文,割石博之
  • Organizer: 第54回リグニン討論会
  • Place of Presentation: 静岡
  • Year and Date: 2009-10-29
• [Presentation] Aspergillus oryzaeのシトクロムP450モノオキシゲナーゼ多様性 (2009)
  • Author(s): 一瀬博文, ら
  • Organizer: 日本生物工学会61回大会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-09-24
• [Presentation] 15. Molecular characterization and isolation of cytochrome P450 genes from Aspergillus oryzae (2009)
  • Author(s): Nazir KHMN. H., Ichinose H., Wariishi H.
  • Organizer: 6th International Conference on Cytochrome P450
  • Place of Presentation: Okinawa Japan
  • Year and Date: 2009-06-21
• [Presentation] Gene expression profiling and enzymatic function of Phanerochaete chrysosporium cytochrome P450 involved in xenobiotic metabolism of aromatic compounds (2009)
  • Author(s): Chigu N. L., Ichinose H., Wariishi H.
  • Organizer: 16th International Conference on Cytochrome P450
  • Place of Presentation: Okinawa Japan
  • Year and Date: 2009-06-21
• [Presentation] Functional characterization of cytochrome P450 from the white-rot fungus Phanerochaete chrysosporium (2009)
  • Author(s): Ichinose H., Hirosue H., Tazaki H., Arisawa H., Jodo H., Wariishi H.
  • Organizer: 16th International Conference on Cytochrome P450
  • Place of Presentation: Okinawa Japan
  • Year and Date: 2009-06-21
• [Presentation] Functional characterization of cytochrome P450 from the white-rot fungus Phanerochaete chrysosporium (2009)
  • Author(s): Ichinose, et al.
  • Organizer: 16^<th> International conference on cytochrome P450
  • Place of Presentation: Okinawa
  • Year and Date: 2009-06-21
• [Presentation] Gene expression profiling and enzymatic function of Phanerochaete chrysosporium cytochrome P450 involved in xenobiotic metabolism of aromatic compounds (2009)
  • Author(s): Chigu, et al.
  • Organizer: 16^<th> International conference on cytochrome P450
  • Place of Presentation: Okinawa
  • Year and Date: 2009-06-21
• [Presentation] Molecular characterization and isolation of cytochrome P450 genes from Aspergillus oryzae (2009)
  • Author(s): Nazir, et al.
  • Organizer: 16^<th> International conference on cytochrome P450
  • Place of Presentation: Okinawa
  • Year and Date: 2009-06-21
• [Remarks] ホームページ等
  • URL: http://brc.wood.agr.kyushu-u.ac.jp/madlab/