Identification of stem cell niche in periodontal skeleton and its molecular regulatory mechanisms.

• Project/Area Number: 21689051
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Orthodontic/Pediatric dentistry
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: ONO Noriaki 東京医科歯科大学, 大学院・医歯学総合研究科, 非常勤講師 (20451908)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥26,130,000 (Direct Cost: ¥20,100,000、Indirect Cost: ¥6,030,000); Fiscal Year 2011: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000); Fiscal Year 2010: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000); Fiscal Year 2009: ¥11,830,000 (Direct Cost: ¥9,100,000、Indirect Cost: ¥2,730,000)
• Keywords: 幹細胞 / 幹細胞ニッチ / 歯周組織 / 歯根形成 / 内軟骨性骨化 / 傍軟骨膜 / 血管内皮 / 微小血管 / 骨芽細胞 / 間葉系幹細胞 / 骨芽細胞系前駆細胞 / Nestin / Osterix / 骨原基初期発生 / 軟骨膜 / 成長板軟骨 / 組織特異的幹細胞 / 骨格系 / 長期ラベル保持能 / CreERTシステム / Tet-Onシステム / BACトランスジェニック / Msx2 / Runx2

Research Abstract

How putative stem-like cells residing in the periodontal and craniofacial skeleton are generated has been incompletely understood. Compound transgenic mice harboring an inducible Cre recombinase and a fluorescent reporter were used for the purpose of lineage-tracing experiments. The preliminary data suggest that early cells involving skeletogenesis are highly heterogeneous and comprised of a wide spectrum of cells including endothelial cells. In addition, each subset of progenitors is destined for different fate and functionality during development and regeneration.

Research Products

• [Journal Article] Constitutively active PTH/PTHRP receptor specifically expressed in osteoblasts enhances bone formation induced by bone marrow ablation (2012)
  • Author(s): Ono, N., Nakashima, K., Schipani, E., Hayata, T., Ezura, Y., Soma, K., Kronenberg, H. M., Noda, M.
  • Journal Title: Journal of Cellular Physiology Volume: 227(2) Pages: 408-15
  • Peer Reviewed
• [Journal Article] A Novel Population of Cells Expressing Both Hematopoietic and Mesenchymal Markers Is Present in the Normal Adult Bone Marrow and Is Augmented in a Murine Model of Marrow Fibrosis (2012)
  • Author(s): Ohishi M, Ono W, Ono N, Khatri R, Marzia M, Baker EK, Root SH, Wilson TL, Iwamoto Y, Kronenberg HM, Aguila HL, Purton LE, Schipani E.
  • Journal Title: American Journal of Pathology Volume: 180(2) Pages: 811-8
  • Peer Reviewed
• [Journal Article] A novel population of cells expressing both hematopoietic and mesenchymal markers is present in the normal adult bone marrow and is augmented in a murine model of marrow fibrosis (2012)
  • Author(s): Ohishi M, Ono W, Ono N, Khatri R, Marzia M, Baker EK, Root SH, Wilson TL, Iwamoto Y, Kronenberg HM, Aguila HL, Purton LE, Schipani E
  • Journal Title: American Journal of Pathology Volume: 180 Issue: 2 Pages: 811-818
  • DOI: 10.1016/j.ajpath.2011.10.028
  • Peer Reviewed
• [Journal Article] Constitutively active PTH/PTHrP receptor specifically expressed in osteoblasts enhances bone formation induced by bone marrow ablation (2012)
  • Author(s): Ono N, Nakashima K, Schipani E, Hayata T, Ezura Y, Soma K, Kronenberg HM, Noda M
  • Journal Title: Journal of Cellular Physiology Volume: 227 Issue: 2 Pages: 408-415
  • DOI: 10.1002/jcp.22986
  • Peer Reviewed
• [Journal Article] Osteopontin deficiency enhances parathyroid hormone/parathyroid hormone related peptide receptor(PPR) signaling-induced alteration in toothformation and odontoblastic morphology (2011)
  • Author(s): Morishita, M., Ono, N., Miyai, K., Nakagawa, T., Hanyu, R., Nagao, M., Kamolratanakul, P., Notomi, T., Rittling, S. R., Denhardt, D. T., Kronenberg, H. M., Ezura, Y., Hayata, T., Nakamoto, T., Noda, M.
  • Journal Title: Tissue & Cell Volume: 43(3) Pages: 196-200
  • Peer Reviewed
• [Presentation] A Novel Double Fluorescent Strategy to Prospectively Isolate Label Retaining Chondrocytes at the Top of the Growth Plate (2011)
  • Author(s): Ono N
  • Organizer: American Society for Bone and Mineral Research
  • Place of Presentation: サンディエゴ
  • Year and Date: 2011-09-18
• [Presentation] A Novel Double Fluorescent Strategy to Prospectively Isolate Label Retaining Chondrocytes at the Top of the Growth Plate (2011)
  • Author(s): Ono N, Hirai T, Kronenberg HM
  • Organizer: American Society for Bone and Mineral Research, 33th Annual Meeting
  • Place of Presentation: San Diego
• [Presentation] Defining distinct microvasculature-associated cell populations in the craniofacial skeleton (2010)
  • Author(s): Noriaki Ono
  • Organizer: The 36th Annual International Conference on Craniofacial Research
  • Place of Presentation: ミシガン大学米国ミシガン州アナーバー
  • Year and Date: 2010-02-26
• [Presentation] Identification of a Novel Cell Population in the Adult Mouse Bone Marrow (2010)
  • Author(s): Ono W, Ohishi M, Ono N, Khatri R, Kronenberg HM, Aguila H, Purton L, Schipani E
  • Organizer: American Society for Bone and Mineral Research, 32nd Annual Meeting
  • Place of Presentation: Toronto