Analysis of O-glycosylated α-synuclein

• Project/Area Number: 21700395
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Nerve anatomy/Neuropathology
• Research Institution: Juntendo University
• Principal Investigator: SHIMURA Hideki Juntendo University, 医学部, 准教授 (50286746)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: パーキンソン病 / O結合型糖化αシヌクレイン / α-シヌクレイン / O-結合型糖化α-シヌクレイン / O-結合型糖鎖 / NeuNAc α2-3Ga1 β1-3GalNAc α / agaricus bisporus lectin / 糖鎖 / レクチン / Q-TOF MS / MALDI-TOF MS / 凝集体

Research Abstract

We investigated O-glycosylatedα-synuclein by biochemical analysis. We identified NeuNAcα2-3Galβ1-3GalNAcαattached to 33Thr, 44Thr, 54Thr residues ofα-synuclein.

Research Products

• [Journal Article] A case of cerebral embolism with metastatic chondrosarcoma in the left atrium. (2011)
  • Author(s): Oizumi H, Tanaka R, Shimura H, Sasaki K, Koike H, Hattori N, Tanaka S.
  • Journal Title: J Stroke Cerebrovasc Dis. 20(1) Pages: 79-81
• [Journal Article] Phosphodiesterase III inhibition promotes differentiation and survival of oligodendrocyte progenitors and enhances regeneration of ischemic white matter lesions in the adult mammalian brain. (2010)
  • Author(s): Miyamoto N, Tanaka R, Shimura H, Watanabe T, Mori H, Onodera M, Mochizuki H,Hattori N, Urabe T.
  • Journal Title: J Cereb Blood Flow Metab. 30(2) Pages: 299-310
• [Journal Article] Predominant Vasogenic Edema in a Patient with Fatal Cerebral Air Embolism. (2010)
  • Author(s): Tanaka R, Shimada Y, Shimura H, Oizumi H, Hattori N, Tanaka S.
  • Journal Title: J Stroke Cerebrovasc Dis.
• [Journal Article] Successful treatment with Yokukansan for behavioral and psychological symptoms of Parkinsonian dementia. (2010)
  • Author(s): Kawanabe T, Yoritaka A, Shimura H, Oizumi H, Tanaka S, Hattori N.
  • Journal Title: Prog Neuropsychopharmacol Biol Psychiatry. 34(2) Pages: 284-287
• [Journal Article] Phosphodiesterase III inhibition promotes differentiation and survival of oligodendrocyte progenitors and enhances regeneration of ischemic white matter lesions in the adult mammalian brain. (2010)
  • Author(s): Miyamoto N, Tanaka R, Shimura H, Watanabe T, Mori H, Onodera M, Mochizuki H, Hattori N, Urabe T.
  • Journal Title: J Cereb Blood Flow Metab. 30(2) Pages: 299-310
• [Journal Article] Phosphodiesterase III inhibition promotes differentiation and survival of oligodendrocyte progenitors and enhances regeneration of ischemic white matter lesions in the adult mammalian brain. (2010)
  • Author(s): Miyamoto N, Tanaka R, Shimura H, Watanabe T, Mori H, Onodera M, Mochizuki H, Hattori N, Urabe T., Miyamoto N, Tanaka R, Shimura H, Watanabe T, Mori H, Onodera M, Mochizuki H, Hattori N, Urabe T.
  • Journal Title: J Cereb Blood Flow Metab Volume: 30 Pages: 299-310
  • Peer Reviewed
• [Journal Article] Economy Class Stroke Syndrome after a Long Drive. (2010)
  • Author(s): Tanaka R, Kawanabe T, Yamauchi Y, Shimura H, Tanaka Y, Miyamoto N, Ueno Y, Urabe T, Hattori N, Tanaka S.
  • Journal Title: J Stroke Cerebrovasc Dis.
  • Peer Reviewed
• [Journal Article] A case of cerebral embolism with metastatic chondrosarcoma in the left atrium. (2010)
  • Author(s): Tanaka R, Shimada Y, Shimura H, Oizumi H, Hattori N, Tanaka S.
  • Journal Title: J Stroke Cerebrovasc Dis.
  • Peer Reviewed
• [Journal Article] Excitatory amino acid transporter 2 associates with phosphorylated tau and is localized in neurofibrillary tangles of tauopathic brains. (2009)
  • Author(s): Sasaki K, Shimura H, Itaya M, Tanaka R, Mori H, Mizuno Y, Kosik KS, Tanaka S, Hattori N.
  • Journal Title: FEBS Lett. 583(13) Pages: 2194-2200
• [Journal Article] Crucial role for Ser133-phosphorylated form of cyclic AMP-responsiveelement binding protein signaling in the differentiation and survival of neural progenitors under chronic cerebral hypoperfusion. (2009)
  • Author(s): Miyamoto N, Tanaka R, Zhang N, Shimura H, Onodera M, Mochizuki H, Hattori N, Urabe T.
  • Journal Title: Neuroscience. 162(2) Pages: 525-536
• [Journal Article] Excitatory amino acid transporter 2 associates with phosphorylated tau and is localized in neurofibrillary tangles of tauopathic brains. (2009)
  • Author(s): Sasaki K
  • Journal Title: FEBS Lett 583 Pages: 2194-200
  • Peer Reviewed
• [Journal Article] Crucial role for Ser133-phosphorylated form of cyclic AMP-responsive element binding protein signaling in the differentiation and survival of neural progenitors under chronic cerebral hypoperfusion. (2009)
  • Author(s): Miyamoto N
  • Journal Title: Neuroscience 162 Pages: 525-36
  • Peer Reviewed
• [Journal Article] Economy Class Stroke Syndrome after a Long Drive.
  • Author(s): Tanaka R, Kawanabe T, Yamauchi Y, Shimura H, Tanaka Y, Miyamoto N, Ueno Y,Urabe T, Hattori N, Tanaka S.
  • Journal Title: J Stroke Cerebrovasc Dis. 2010
• [Journal Article] Phosphodiesterase III inhibition promotes differentiation and survival of oligodendrocyte progenitors and enhances regeneration of ischemic white matter lesions in the adult mammalian brain.
  • Author(s): Miyamoto N
  • Journal Title: J Cereb Blood Flow Metab (印刷中)
  • Peer Reviewed
• [Presentation] O-結合型糖化α-synucleinの生化学的解 (2010)
  • Author(s): 志村秀樹、佐々木久美、Schlossmacher Michael、Selkoe Dennis、服部信考
  • Organizer: 日本神経学会総会
  • Place of Presentation: 東京
• [Presentation] 0結合型糖化α-synucleinの生化学的解析 (2009)
  • Author(s): 志村秀樹
  • Organizer: 日本神経学会
  • Place of Presentation: 仙台
  • Year and Date: 2009-05-21
• [Remarks] ホームページ等