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Regulation of synaptic plasticity by receptor-like protein tyrosine phosphatase

Research Project

Project/Area Number 21700416
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Neurochemistry/Neuropharmacology
Research InstitutionNational Institute for Basic Biology

Principal Investigator

FUJIKAWA Akihiro  National Institute for Basic Biology, 統合神経生物学研究部門, 特別協力研究員 (50414016)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords神経科学 / 生体分子 / 蛋白質 / 脳・神経
Research Abstract

The extracellular region of Ptprz is shedded by MMP-9 or plasmin, producing its membrane-tethered fragment. Both proteases are reportedly activated when synaptic remodeling is occurring. We revealed that Ptprz fragments are accumulated in the post synaptic density PSD fraction, indicating that this receptor is actually cleaved at central synapses. We identified the dephosphorylation sites in Git1 and Magi1. Alignment of the primary sequences surrounding the target phospho-tyrosine residue in these substrate proteins showed a considerable similarity, suggesting a consensus motif for substrate recognition by Ptprz. We found that the substrate motif thus deduced is present in some proteins, including at Tyr118 of paxillin. Finally, we verified that Ptprz efficiently dephosphorylates paxillin at this site in cultured cells.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (6 results)

All 2011 2010 Other

All Presentation (1 results) Book (2 results) Remarks (1 results) Patent(Industrial Property Rights) (2 results)

  • [Presentation] Consensus substrate sequence for Ptprz2011

    • Author(s)
      藤川顕寛
    • Organizer
      第9回国際プロテインホスファターゼカンファレンス
    • Place of Presentation
      東京大学(東京都)
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Book] 細胞工学2011

    • Author(s)
      藤川顕寛, 野田昌晴
    • Publisher
      秀潤社学研メディカル
    • Related Report
      2010 Final Research Report
  • [Book] 細胞工学2011

    • Author(s)
      藤川顕寛・野田昌晴
    • Total Pages
      5
    • Publisher
      学研メディカル秀潤社
    • Related Report
      2010 Annual Research Report
  • [Remarks] ホームページ等

    • Related Report
      2010 Final Research Report
  • [Patent(Industrial Property Rights)] 受容体型チロシンホスファターゼPtprzの基質モチーフ及びその用途2010

    • Inventor(s)
      藤川顕寛, 野田昌晴
    • Industrial Property Rights Holder
      自然科学研究機構
    • Filing Date
      2010-02-26
    • Related Report
      2010 Final Research Report
  • [Patent(Industrial Property Rights)] 受容体型チロシンホスファターゼPtprzの基質モチーフ及びその用途2010

    • Inventor(s)
      藤川顕寛, 野田昌晴
    • Industrial Property Rights Holder
      自然科学研究機構
    • Filing Date
      2010-02-26
    • Related Report
      2010 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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