Establishment of the rabbit pluripotent stem cell systems for compensating for the difference between human and mouse stem cells.

• Project/Area Number: 21700453
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Laboratory animal science
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: HONDA Arata The Institute of Physical and Chemical Research, 遺伝工学基盤技術室, 客員研究員 (10373367)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: ウサギ / 多能性幹細胞 / ES細胞 / iPS細胞 / 核移植クローン / 再生医療

Research Abstract

I identified that bFGF can maintain the undifferentiated status of rabbit ES cells and found that Activin signaling through Smad2/3 activation is necessary to maintain the pluripotent status of rabbit ES cells as those of human ES cells. Moreover, I successfully generated rabbit induced pluripotent stem cells.

Research Products

• [Journal Article] Generation of induced pluripotent stem cells in rabbits : potential experimental models for human regenerative medicine. (2010)
  • Author(s): Honda A, Hirose M, Hatori M, Matoba A, Miyoshi H, Inoue K, Ogura A.
  • Journal Title: J.Biol.Chem. (in press.)
  • Peer Reviewed
• [Journal Article] ウサギ多能性幹細胞とその可能性 (2010)
  • Author(s): 本多新
  • Journal Title: アニテックス 22 Pages: 26-31
• [Journal Article] Generation of induced pluripotent stem cells in rabbits : potential experimental models for human regenerative medicine. (2010)
  • Author(s): Arata Honda
  • Journal Title: Journal of Biological Chemistry Volume: 285 Pages: 31362-31369
  • Peer Reviewed
• [Journal Article] ウサギ多能性幹細胞とその可能性 (2010)
  • Author(s): 本多新
  • Journal Title: アニテックス Volume: 22 Pages: 26-31
• [Journal Article] Basic FGF and Activin/Nodal but not LIF signaling sustain undifferentiated status of rabbit embryonic stem cells. (2009)
  • Author(s): Honda A, Hirose M, Ogura A.
  • Journal Title: Exp.CellRes. 315 Pages: 2033-2042
  • Peer Reviewed
• [Presentation] ウサギ多能性幹細胞とその可能性 (2011)
  • Author(s): 本多新
  • Organizer: CREST/さきがけ「iPS細胞」研究領域合同シンポジウム2010
  • Place of Presentation: 東京都江東区
  • Year and Date: 2011-01-14
• [Presentation] Generation of induced pluripotent stem cells in rabbits (2010)
  • Author(s): Arata Honda
  • Organizer: 第33回日本分子生物学会・第83回日本生化学会大会合同大会
  • Place of Presentation: 兵庫県神戸市
  • Year and Date: 2010-12-10
• [Presentation] ウサギ多能性幹細胞の樹立とその解析 (2010)
  • Author(s): 本多新
  • Organizer: 第四回ウサギフォーラム
  • Place of Presentation: 秋田
  • Year and Date: 2010-07-24
• [Presentation] ウサギ多能性幹細胞の樹立とその解析 (2010)
  • Author(s): 本多新
  • Organizer: 第四回ウサギフォーラム
  • Place of Presentation: 秋田県秋田市
  • Year and Date: 2010-07-24
• [Presentation] ウサギ多能性幹細胞とその可能性 (2010)
  • Author(s): 本多新
  • Organizer: 第36回重粒子医科学センター研究交流会
  • Place of Presentation: 千葉
  • Year and Date: 2010-05-13
• [Presentation] Generation of induced pluripotent stem cells in rabbits. (2010)
  • Author(s): Honda A, Hirose M, Hatori M, Matoba A, Miyoshi H, Inoue K, Ogura A.
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] ウサギES細胞の樹立・維持に影響を及ぼす因子とシグナル伝達経路の解析 (2009)
  • Author(s): 本多新
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜(パシフィコ横浜)
  • Year and Date: 2009-12-09
• [Presentation] Current status of rabbit ES cells : where are we and where can we go? (2009)
  • Author(s): Honda A.
  • Organizer: The Third International Meeting on Rabbit Biotechnology.
  • Place of Presentation: 中国(西安)
  • Year and Date: 2009-06-24
• [Presentation] Current status of rabbit ES cells : where are we and where can we go? (2009)
  • Author(s): 本多新
  • Organizer: The Third International Meeting on Rabbit Biotechnology
  • Place of Presentation: 中国(西安)
  • Year and Date: 2009-06-08
• [Presentation] ウサギES細胞の樹立・維持に影響を及ぼす因子とシグナル伝達経路の解析 (2009)
  • Author(s): 本多新、廣瀬美智子、小倉淳郎
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] ウサギES細胞の樹立と未分化生維持機構の解析 (2009)
  • Author(s): 廣瀬美智子、本多新、小倉淳郎
  • Organizer: 第56回日本実験動物学会総会
  • Place of Presentation: さいたま
• [Remarks] ホームページ等