Development of magnetic and optical nanodiamond implanted with paramagnetic ions

• Project/Area Number: 21700465
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Osaka University
• Principal Investigator: MORITA Masahito Osaka University, 免疫学フロンティア研究センター, 特任助教 (30381594)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: ナノバイオロジー / ナノメディスン / マルチモーダル造影剤 / 低侵襲医療 / ナノダイヤモンド / MRI / イオン注入 / 金ナノ粒子

Research Abstract

We succeeded in the synthesis of multimodal nanodiamond responded to the magnetic field and visible light. The attachment of gold nanoparticles on the multimodal nanodiamond resulted in the stable disperse in a physiological condition. A mouse liver was visualized 6 and 24 hours later after the administration of this PEG-Au-Mn-ND into mouse tail vein. Further TEM analysis revealed that these hybrid nanomaterials were distributed in liver tissue including Kupper cells. These results imply that the paramagnetic-ion implanted nanodimoand have the potential to be used for long-term in vivo imaging experiments, including cell tracking, metabolic change etc. in the future.

Research Products

• [Journal Article] Preparation of Fluorescent Diamond Nanoparticles Stably Dispersed under a Physiological Environment through Multistep Organic Transformations. (2010)
  • Author(s): Takimoto T, Chano T, Shimizu S, Okabe H, Ito M, Morita M, Kimura T, Inubushi T, Komatsu N.
  • Journal Title: Chemistry of Materials 22 Pages: 3462-3471
  • Peer Reviewed
• [Journal Article] Preparation for highly sensitive MRI contrast agents using core/shell type nanoparticles consisting of multiple SPIO cores with thin silica coating. (2010)
  • Author(s): Tanaka K, Narita A, Kitamura N, Uchiyama W, Morita M, Inubushi T, Chujo Y.
  • Journal Title: Langmuir 26 Pages: 11759-11762
  • Peer Reviewed
• [Journal Article] Simple PEG conjugation of SPIO via an Au-S bond improves its tumor targeting potency as a novel MR tumor imaging agent. (2010)
  • Author(s): Kojima H, Mukai Y, Yoshikawa M, Kamei K, Yoshikawa T, Morita M, Inubushi T, Yamamoto TA, Yoshioka Y, Okada N, Seino S, Nakagawa S.
  • Journal Title: Bioconjug Chem. 21 Pages: 1026-1031
  • Peer Reviewed
• [Journal Article] Simple PEG conjugation of SPIO via an Au-S bond improves its tumor targeting potency as a novel MR tumor imaging agent (2010)
  • Author(s): Kojima H, Mukai Y, Yoshikawa M, Kamei K, Yoshikawa T, Morita M, Inubushi T, Yamamoto TA, Yoshioka Y, Okada N, Seino S, Nakagawa S.
  • Journal Title: Bioconjug Chem. Volume: 21 Pages: 1026-1031
  • Peer Reviewed
• [Journal Article] Preparation of Fluorescent Diamond Nanoparticles Stably Dispersed under a Physiological Environment through Multistep Organic Transformations. (2010)
  • Author(s): Takimoto T, Chano T, Shimizu S, Okabe H, Ito M, Morita M, Kimura T, Inbushi T, Komatsu N.
  • Journal Title: Chemistry of Materials Volume: 22 Pages: 3462-3471
  • Peer Reviewed
• [Journal Article] Preparation for Highly Sensitive MRI Contrast Agents Using Core/Shell Type Nanoparticles Consisting of Multiple SPIO Cores with Thin Silica Coating (2010)
  • Author(s): Tanaka K, Narita A, Kitamura N, Uchiyama W, Morita M, Inubushi T, Chujo Y
  • Journal Title: Langmuir Volume: 26 Pages: 11759-11762
  • Peer Reviewed
• [Journal Article] Improving Proton Relaxivity of Dendritic MRI Contrast Agents by Rigid Silsesquioxane Core. (2009)
  • Author(s): Tanaka, K., Kitamura, N., Naka, K., Morita, M., Inubushi, T., Chujo, M.Nagao, M., Chujo, Y
  • Journal Title: Polymer J. 41 Pages: 287-292
  • NAID: 10025091393
  • Peer Reviewed
• [Presentation] Magneto-Optical imaging probes for visualization of dynamic biological responses using MRI/fluorescent imaging. (2010)
  • Author(s): Morita M
  • Organizer: Japan-France Frontiers of Engineering1st JFFoE symposium
  • Place of Presentation: グルノーブル・フランス
  • Year and Date: 2010-10-12
• [Presentation] Mn-doped nanodiamond for MRI imaging probes. (2010)
  • Author(s): Morita M, Y Harada, Y Tateyama, S Shin, M Oshima, S Nagamachi, N Komatsu, T Inubushi, Y Yoshioka
  • Organizer: Diamond2010
  • Place of Presentation: ブダペスト・ハンガリー
  • Year and Date: 2010-09-09
• [Presentation] Mn doped magnetic nanodiamond for MRI imaging probes (2010)
  • Author(s): Morita M
  • Organizer: Daimond2010 international conference
  • Place of Presentation: Budapest, Hungary
  • Year and Date: 2010-09-08
• [Presentation] 分子・細胞イメージングのためのマルチモーダルイメージング技術 (2009)
  • Author(s): 森田将史
  • Organizer: 第82回日本生化学会学会シンポジウム
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-21
• [Presentation] 分子・細胞イメージングのためのマルチモーダルイメージング技術 (2009)
  • Author(s): 森田将史
  • Organizer: 第82回日本生化学会学会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2009-10-21
• [Presentation] MRI and MRS for visualization of immune responses、"Frontier Immuno-Imaging" (2009)
  • Author(s): Morita M
  • Organizer: International Symposium organized by JST & IFReC
  • Place of Presentation: 大阪
  • Year and Date: 2009-05-11
• [Remarks] ホームページ等
  • URL: http://biofunc.ifrec.osaka-u.ac.jp/
• [Patent(Industrial Property Rights)] MRI造影剤 (2010)
  • Inventor(s): 森田将史、清野智史、向洋平、中川貴、中川晋作
  • Industrial Property Rights Holder: 大阪大学
  • Industrial Property Number: 2010-041460
  • Filing Date: 2010-02-26