Facile and rapid detection of toxic heavy metal ions using double-stranded DNA-gold nanoparticle conjugates containing mismatch base pair

• Project/Area Number: 21700495
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biomedical engineering/Biological material science
• Research Institution: University of Tsukuba
• Principal Investigator: KANAYAMA Naoki University of Tsukuba, 大学院・数理物質科学研究科, 講師 (80377811)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
• Keywords: DNA / 金ナノ粒子 / 重金属イオン / ミスマッチ塩基対 / 非架橋凝集

Research Abstract

Colorimetric detection of mercury ions (Hg^<2+>) with the naked eye was accomplished by a combination of non-crosslinking aggregation of double-stranded DNA-carrying gold nanoparticles (dsDNA-GNP) and complex formation of thymine-Hg^<2+>-thymine (T-Hg^<2+>-T). The dsDNA-GNP having a T-T mismatch site located near the distal end aggregated spontaneously within 1 min in the presence of Hg(ClO_4)_2 at a sub-micromolar concentration in aqueous media at room temperature, resulting in the distinct color change of the particle's dispersion from red to colorless. This unique colloidal behavior was presumably due to the decrease in the entropic repulsion between the particles induced by the T-Hg^<2+>-T complex formation at the interface between the dsDNA layer and the dispersal medium.

Research Products

• [Journal Article] Rapid naked-eye detection of mercury ion based on non-crosslinking aggregation of double-stranded DNA carrying gold nanoparticles (2011)
  • Author(s): Naoki Kanayama、Tohru Takarada、Mizuo Maeda
  • Journal Title: Chemical Communications Vol.47 Pages: 2077-2079
  • Peer Reviewed
• [Journal Article] Rapid naked-eye detection of mercury ions based on non-crosslinking aggregation of double-stranded DNA-carrying gold nanoparticles (2011)
  • Author(s): Naoki Kanayama, Tohru Takarada, Mizuo Maeda
  • Journal Title: Chemical Communications Volume: 47 Pages: 2077-2079
  • Peer Reviewed
• [Journal Article] Facile creation of biointerface on commodity plastic surface by combination of atmospheric plasma and reactive polymer coating (2010)
  • Author(s): Naoki Kanayama、Swapan Kumar Saha、Naoki Nakayama、Jun Nakanishi、Katsuhisa Kitano、Satoshi Hamaguchi、Yukio Nagasaki
  • Journal Title: Journal of Photopolymer Science and Technology Vol.23 Pages: 579-583
  • NAID: 130004833370
  • Peer Reviewed
• [Journal Article] DNAコンジュゲート材料によるバイオ計測法の開発 (2010)
  • Author(s): 金山直樹、前田瑞夫
  • Journal Title: ファルマシア 46巻 Pages: 317-322
• [Journal Article] DNAコンジュゲート材料によるバイオ計測法の開発 (2010)
  • Author(s): 金山直樹、前田瑞夫
  • Journal Title: ファルマシア(日本薬学会誌) 46 Pages: 317-322
• [Journal Article] Facile Creation of Biointerface on Commodity Plastic Surface by Combination of Atmospheric Plasma and Reactive Polymer Coating (2010)
  • Author(s): Naoki Kanayama, Swapan Kumar Saha, Naoki Nakayama, Jun Nakanishi, Katsuhisa Kitano, Satoshi Hamaguchi, Yukio Nagasaki
  • Journal Title: Journal of Photopolymer Science and Technology (印刷中)
  • NAID: 130004833370
  • Peer Reviewed
• [Presentation] Facile and rapid naked-eye detection of mercury (II) ions using non-crosslinking aggregation behavior of double-stranded DNA carrying gold nanoparticles (2010)
  • Author(s): Naoki Kanayama、Tohru Takarada、Mizuo Maeda
  • Organizer: The 37^<th> International Symposium on Nucleic Acids Chemistry 2010 (ISNAC2010)
  • Place of Presentation: Yokohama
  • Year and Date: 2010-11-10
• [Presentation] Facile and rapid naked eye-detection of mercury(II) ions using non-crosslinking a ggregation behavior of double-stranded DNA carrying gold nanoparticles (2010)
  • Author(s): Naoki Kanayama, Tohru Takarada, Mizuo Maeda
  • Organizer: The 37th International Symposium on Nucleic Acids Chemistry 2010 (ISNAC2010)
  • Place of Presentation: はまぎんホール(横浜市)
  • Year and Date: 2010-11-10
• [Presentation] DNA二重鎖の"柔らかさ"に着目した水銀イオン検出法 (2010)
  • Author(s): 金山直樹、宝田徹、前田瑞夫
  • Organizer: 第25回高分子学会関東支部茨城地区若手の会交流会
  • Place of Presentation: つくば
  • Year and Date: 2010-10-27
• [Presentation] DNA二重鎖末端の"柔らかさ"に着目した水銀イオン検出法 (2010)
  • Author(s): 金山直樹、宝田徹、前田瑞夫
  • Organizer: 第25回高分子学会関東支部茨城地区若手の会交流会
  • Place of Presentation: つくばセミナーハウス(つくば市)
  • Year and Date: 2010-10-27
• [Presentation] DNA鎖で金ナノ粒子を躾る～水銀イオンセンサーへの応用～ (2009)
  • Author(s): 金山直樹、宝田徹、前田瑞夫
  • Organizer: 第24回高分子学会関東支部茨城地区若手の会交流会
  • Place of Presentation: つくば
  • Year and Date: 2009-10-29
• [Presentation] DNA鎖で金ナノ粒子を躾る～水銀イオンセンサーへの応用～ (2009)
  • Author(s): 金山直樹、宝田徹、前田瑞夫
  • Organizer: 高分子学会関東支部第24回茨城地区若手の会交流会
  • Place of Presentation: つくばセミナーハウス
  • Year and Date: 2009-10-29
• [Presentation] ミスマッチペアを導入したDNA二重鎖担持金ナノ粒子を用いる水銀(II)イオンの簡便・迅速検出 (2009)
  • Author(s): 金山直樹、宝田徹、前田瑞夫
  • Organizer: 第58回高分子討論会
  • Place of Presentation: 熊本
  • Year and Date: 2009-09-16
• [Presentation] ミスマッチペアを導入したDNA二重鎖担持金ナノ粒子を用いる水銀(II)イオンの簡便・迅速検出 (2009)
  • Author(s): 金山直樹、宝田徹、前田瑞夫
  • Organizer: 第58回高分子討論会
  • Place of Presentation: 熊本大学
  • Year and Date: 2009-09-16
• [Presentation] DNA二重鎖を担持した金ナノ粒子を用いる水銀(II)イオンの簡便・迅速検出 (2009)
  • Author(s): 金山直樹、宝田徹、前田瑞夫
  • Organizer: 東京コンファレンス
  • Place of Presentation: 幕張
  • Year and Date: 2009-09-04
• [Presentation] DNA二重鎖を担持した金ナノ粒子を用いる水銀(II)イオンの簡便・迅速検出 (2009)
  • Author(s): 金山直樹、宝田徹、前田瑞夫
  • Organizer: 東京コンファレンス2009
  • Place of Presentation: 幕張メッセ国際会議場
  • Year and Date: 2009-09-04