Development of Clathrate-type single-cell array chip using thermoresponsive polymer

• Project/Area Number: 21700496
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Toyama Industrial Technology Center,
• Principal Investigator: YOKOYAMA Yoshiyuki 富山県工業技術センター, 機械電子研究所, 主任研究員 (60416154)
• Project Period (FY): 2009 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 温度応答性高分子 / ゲル / 細胞アレイチップ / フォトリソグラフィー / ナノインプリント / バイオレジスト / 微細加工 / 細胞チップ / 1細胞操作 / 細胞モップ

Research Abstract

We developed Bio-resist by providing thermoresponsive polymer with photocrosslinking and thermal cross-linking capabilities. The Bio-resist can be used for micro fabrication by photolithography and thermal-nanoimprint. And small Bio-resist patterns could be changed reversibly by changing temperature. Moreover, we developed a clathrate-type single-cell array chip which consists of many arrays of Bio-resist hole patterns where small objects such as single-cells can be captured and released.

Research Products

• [Journal Article] Micro- and nano-fabrication of stimulus-responsive polymer using nanoimprint lithography (2011)
  • Author(s): Y. Yokoyama, M. Umezaki, T. Kishioka, E. Tamiya and Y. Takamura
  • Journal Title: Journal of Photopolymer Science and Technology Volume: Vol.24 Pages: 63-70
  • NAID: 40019008720
  • Peer Reviewed
• [Journal Article] Micro- and nano-fabrication of stimulus-responsive polymer using nanoimprint lithography (2011)
  • Author(s): Y.Yokoyama, M.Umezaki, T.Kishioka, E.Tamiya, Y.Takamura
  • Journal Title: Journal of Photopolymer Science and Technology Volume: 24 Pages: 63-70
  • NAID: 40019008720
  • Peer Reviewed
• [Presentation] Clathrate-type cell array chip that can freely capture, release and array cells using thermoresponsive gel (2011)
  • Author(s): Y.Yokoyama, M.Umezaki, T.Kishioka, Y.Takamura
  • Organizer: International Conference on Biofabrication 2011 in TOYAMA
  • Place of Presentation: 富山国際会議場
  • Year and Date: 2011-10-06
• [Presentation] Micro- and nano-fabrication of stimulus-responsive polymer using nanoimprint lithography (2011)
  • Author(s): Y.Yokoyama, M.Umezaki, T.Kishioka, E.Tamiya, Y.Takamura
  • Organizer: The 28th International Conference of Photopolymer Science and Technology (ICPST-28)
  • Place of Presentation: 千葉大学けやき会館
  • Year and Date: 2011-06-23
• [Presentation] 温度応答性ポリマーのバイオ応用 (2011)
  • Author(s): 横山義之
  • Organizer: ナノインプリント技術の開発とその応用(主催:応用物理学会 北陸・信越支部)
  • Place of Presentation: 富山国際会議場(富山県)(招待講演)
  • Year and Date: 2011-01-13
• [Presentation] (2011)
  • Organizer: International Conference on Biofabrication 2011
  • Place of Presentation: TOYAMA
• [Presentation] 刺激応答性ポリマーを用いたバイオチップ用レジストの開発 (2010)
  • Author(s): 横山義之
  • Organizer: 第56回高分子討論会
  • Place of Presentation: 北海道大学(北海道)
  • Year and Date: 2010-09-16
• [Presentation] 温度応答性ポリマーのバイォ応用 (2010)
  • Author(s): 横山義之
  • Organizer: 第6回ナノインプリント技術研究会(招待講演)
  • Place of Presentation: 弘済会館(東京都)
  • Year and Date: 2010-03-05
• [Presentation] Development of micro-fabrication technology of thermal-responsive polymer using photolithography and nanoimprint method. (2009)
  • Author(s): 横山義之
  • Organizer: 22nd International Microprocesses and Nanotechnology Conference
  • Place of Presentation: シェラトン札幌(北海道)
  • Year and Date: 2009-11-17
• [Presentation] ナノインプリント法を用いた温度応答性ポリマーの微細加工技術の開発 (2009)
  • Author(s): 横山義之
  • Organizer: 第70回応用物理学会学術講演会
  • Place of Presentation: 富山大学(富山県)
  • Year and Date: 2009-09-09
• [Presentation]
  • Organizer: The 28th International Conference of Photopolymer Science and Technology (ICPST-28)
• [Presentation] ナノインプリント技術の開発とその応用
  • Organizer: 応用物理学会 北陸・信越支部セミナー(招待講演)
• [Presentation]
  • Organizer: 第6回ナノインプリント技術研究会(応用物理学会)(招待講演)
• [Presentation]
  • Organizer: 第56回高分子討論会(依頼講演)
• [Presentation]
  • Organizer: 22nd International Microprocesses and Nanotechnology Conference
• [Presentation]
  • Organizer: 第70回 応用物理学会 学術講演会
• [Book] ナインプリントの開発とデバイス応用 (2011)
  • Author(s): 横山義之、他
  • Publisher: シーエムシー出版
• [Book] コンバーテック (2009)
  • Author(s): 横山義之、他
  • Total Pages: 64
  • Publisher: 加工技術研究会
• [Remarks] (所属機関HP)
  • URL: http://www.itc.pref.toyama.jp/