Clarification of molecular mechanism in prevention of muscular atrophy by transient blood flow restriction.

• Project/Area Number: 21700548
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Rehabilitation science/Welfare engineering
• Research Institution: Juntendo University
• Principal Investigator: KAKEHI Saori Juntendo University, 医学部, 研究員 (00450560)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 筋萎縮 / インスリン抵抗性 / 不活動 / 骨格筋

Research Abstract

Transient blood flow restriction prevents muscular atrophy and weakness induced by chronic unloading. However, it was unclear which molecular mechanism. In cultured C2C12 myotube, the mRNA level of ubiquitin ligase atrogin-1・MuRF1 and the decreasing of diameter were inhibited by hypoxic stimulation at hydrogen peroxide -induced muscle atrophy model. On the other hand, the amount of insulin stimulated glucose uptake was decreased by 24 hours hind limb fixation.

Research Products

• [Journal Article] 長期療養高齢者と高齢者医療を担う医師の役割 (2009)
  • Author(s): 高橋龍太郎、筧佐織
  • Journal Title: 日本老年医学会雑誌 Vol.46 Pages: 134-136
  • NAID: 10024964052
• [Journal Article] Determinants of intramyocellular lipid accumulation after dietary fat loading in non-obese men.
  • Author(s): Sakurai Y, Tamura Y, Takeno K, Kumashiro N, Sato F, Kakehi S, Ikeda S, Katamoto S, Ogura Y, Saga N, Naito H, Fujitani Y, Hirose T, Kawamori R, Watada H
  • Journal Title: J Diabetes Invest. in press
• [Journal Article] Determinants of intramyocellular lipid accumulation after dietary fat loading in non-obese men
  • Author(s): Sakurai Y, Tamura Y, Takeno K, Kumashiro N, Sato F, Kakehi S, Ikeda S, Katamoto S, Ogura Y, Saga N, Naito H, Fujitani Y, Hirose T, Kawamori R, Watada H
  • Journal Title: J Diabetes Invest Volume: (In press)
  • Peer Reviewed
• [Presentation] 筋サイズ並びに筋糖代謝に対するPKCδの関与の検討 (2010)
  • Author(s): 池田真一、田村好史、筧佐織、綿田裕孝、河盛隆造
  • Organizer: 第65回日本体力医学会
• [Presentation] 脂肪負荷感受性の規定因子の解明 (2010)
  • Author(s): 筧佐織、田村好史、池田真一、綿田裕孝、河盛隆造
  • Organizer: 第65回日本体力医学会
• [Presentation] Susceptible Factors for Intramyocellular Lipid Accumulation by Dietary Fat Loading in Non-Obese Men (2010)
  • Author(s): Sakurai Y, Tamura Y, Takeno K, Kumashiro N, Sato F, Ikeda S, Kakehi S, Katamoto S, Naito H, Saga N, Ogura Y, Hirose T, Fujitani Y, Watada H
  • Organizer: American Diabetes Association 70^<th> Scientific sessions
  • Place of Presentation: Orlando
• [Presentation] ヒトにおける高脂肪食負荷が骨格筋細胞内脂質蓄積とインスリン感受性に与える影響とその規定因子の解明 (2010)
  • Author(s): 櫻井裕子, 田村好史, 竹野景海, 熊代尚紀, 佐藤文彦, 池田真一, 筧佐織, 形本静夫, 内藤久士, 小倉裕司, 佐賀典生, 弘世貴久, 綿田裕孝, 河盛隆造
  • Organizer: 第53回日本糖尿病学会年次学術集会
  • Place of Presentation: 岡山県岡山市
• [Presentation] 異所性脂肪蓄積とインスリン抵抗性 (2010)
  • Author(s): 田村好史, 櫻井裕子, 竹野景海, 筧佐織, 池田真一, 川口美奈子, 渡邉隆宏, 佐藤文彦, 河盛隆造, 綿田裕孝
  • Organizer: Advans研究会
  • Place of Presentation: 千葉県木更津市
• [Presentation] 筋インスリン抵抗性に対する高脂肪食と身体活動量の作用 (2009)
  • Author(s): 筧佐織
  • Organizer: 第64回日本体力医学会
  • Place of Presentation: 新潟
  • Year and Date: 2009-09-19
• [Presentation] Effects of high fat diet on intramyoellular lipid and insulin resistance. (2009)
  • Author(s): Tamura Y, Sakurai Y, Takeno K, Kumashiro N, Ikeda S, Kakehi S, Watada H, Kawamori R
  • Organizer: 14^<th> International Congress of Endocrinology
  • Place of Presentation: Kyoto, Japan
• [Presentation] Effects of high fat diet on intramyocellular lipid and insulin resistance. (2009)
  • Author(s): Sakurai Y, Tamura Y, Takeno K, Kumashiro N, Ikeda S, Kakehi S, Watada H, Kawamori R
  • Organizer: The First Asia-Pacific Conference on Health Promotion and Education
  • Place of Presentation: Chiba, Japan
• [Presentation] Diabetes and Exercise : Intramycellular Lipid Accumulation and Insulin Sensitivity. (2009)
  • Author(s): Tamura Y, Sakurai Y, Takeno K, Kakehi S, Ikeda S, Sato F, Hirose T, Watada H, Kawamori R.
  • Organizer: In The 15thKorea-Japan Symposium on Diabetes Mellitus Jeju p.84-85
  • Place of Presentation: Korea
• [Presentation] 廃用性筋萎縮に対するPKCδの関与の検討 (2009)
  • Author(s): 池田真一、田村好史、筧佐織、綿田裕孝、河盛隆造
  • Organizer: 第64回日本体力医学会
• [Presentation] 筋インスリン抵抗性に対する高脂肪食と身体活動量の作用 (2009)
  • Author(s): 筧佐織、田村好史、池田真一、綿田裕孝、河盛隆造
  • Organizer: 第64回日本体力医学会
• [Presentation] ヒトにおける高脂肪食負荷が、骨格筋細胞内脂質蓄積とインスリン抵抗性発生に与える影響とその規定因子 (2009)
  • Author(s): 田村好史、櫻井裕子、筧佐織、池田真一、佐賀典生、小倉裕司、内藤久士、綿田裕孝、河盛隆造
  • Organizer: 第64回日本体力医学会
• [Presentation] ヒトにおける高脂肪食負荷が、骨格筋細胞内脂質蓄積とインスリン抵抗性発症に与える影響とその規定因子 (2009)
  • Author(s): 櫻井裕子, 田村好史, 竹野景海, 熊代尚記, 池田真一, 筧佐織, 綿田裕孝, 河盛隆造
  • Organizer: 第52回日本糖尿病学会年次学術集会
• [Presentation] ヒトにおける高脂肪食負荷が、骨格筋細胞内脂質蓄積とインスリン抵抗性発症に与える影響とその規定因子 (2009)
  • Author(s): 田村好史, 櫻井裕子, 竹野景海, 熊代尚記, 池田真一, 筧佐織, 綿田裕孝, 河盛隆造
  • Organizer: 第59回日本体質医学会総会 東京都千代田区(日本),日本体質医学会